Q12421 (ATG31_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autophagy-related protein 31

Alternative name(s):
CIK1 suppressor protein 1
Protein CIS1

Gene names

Name:	ATG31
Synonyms:	CIS1
Ordered Locus Names:	YDR022C
ORF Names:	PZD196, YD9335.08c

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in starvation-induced autophagy. Involved in mitophagy. Functions with ATG17 and ATG29 at the preautophagosomal structure (PAS) in order to form normal autophagosomes under starvation conditions. May be involved in microtubule function, such as chromosome segregation and karyogamy. Ref.5 Ref.8 Ref.9 Ref.12
Subunit structure	Forms a stable complex with ATG17 and ATG29. Interacts directly with ATG29. The ATG17-ATG29-ATG31 complex interacts with the ATG1-ATG13 complex. Note=The interaction with the ATG1-ATG13 complex is induced by starvation. Ref.8 Ref.9 Ref.10 Ref.11
Subcellular location	Cytoplasm › cytoskeleton Probable. Preautophagosomal structure Ref.6 Ref.8 Ref.9.
Post-translational modification	Phosphorylated. Ref.11
Miscellaneous	Present with 486 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Autophagy Chromosome partition Karyogamy Protein transport Transport
Cellular component	Cytoplasm Cytoskeleton Microtubule
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW karyogamy Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion degradation Inferred from mutant phenotype Ref.12. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype PubMed 18701704. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW pre-autophagosomal structure Inferred from direct assay Ref.8PubMed 18497569. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG17	Q06410	2	EBI-34768,EBI-30856

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Autophagy-related protein 31		PRO_0000076210

Sequences

	Sequence		Length	Mass (Da)
	Q12421 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: CC2396854F29D250	FASTA	196	22,191
	10 20 30 40 50 60 MNVTVTVYDK NVKYRLEENI KNNKGPSNDD QPAYNNESKS TDGSDYAMFP TNIKYIFEDN 70 80 90 100 110 120 NDELVDSSDA ALTAGIDKVG DELENVIIVQ LDESGSLEDI TLISDQYELL SHRTNSLSLE 130 140 150 160 170 180 ENQMRTLSSH GDDKSNDEEE ELSVDSDRFR VDSDIELDVI SQFCDLSPFL RDLSLNDLIK 190 LYVTQNEQLQ MLSNSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 35.4 kb region on the right arm of chromosome IV from Saccharomyces cerevisiae reveal 23 open reading frames." Eide L.G., Sander C., Prydz H. Yeast 12:1085-1090(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The Rho-GEF Rom2p localizes to sites of polarized cell growth and participates in cytoskeletal functions in Saccharomyces cerevisiae." Manning B.D., Padmanabha R., Snyder M. Mol. Biol. Cell 8:1829-1844(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Cis1/Atg31 is required for autophagosome formation in Saccharomyces cerevisiae." Kabeya Y., Kawamata T., Suzuki K., Ohsumi Y. Biochem. Biophys. Res. Commun. 356:405-410(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG17.
[9]	"Organization of the pre-autophagosomal structure responsible for autophagosome formation." Kawamata T., Kamada Y., Kabeya Y., Sekito T., Ohsumi Y. Mol. Biol. Cell 19:2039-2050(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ATG1.
[10]	"A multiple ATG gene knockout strain for yeast two-hybrid analysis." Cao Y., Nair U., Yasumura-Yorimitsu K., Klionsky D.J. Autophagy 5:699-705(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ATG17 AND ATG29.
[11]	"Characterization of the Atg17-Atg29-Atg31 complex specifically required for starvation-induced autophagy in Saccharomyces cerevisiae." Kabeya Y., Noda N.N., Fujioka Y., Suzuki K., Inagaki F., Ohsumi Y. Biochem. Biophys. Res. Commun. 389:612-615(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE ATG17-ATG29-ATG31 COMPLEX, INTERACTION WITH THE ATG1-ATG13 COMPLEX, PHOSPHORYLATION.
[12]	"A genomic screen for yeast mutants defective in selective mitochondria autophagy." Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J. Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X95966 Genomic DNA. Translation: CAA65214.1. Z74318 Genomic DNA. Translation: CAA98843.1. Z49770 Genomic DNA. Translation: CAA89847.1. AY557645 Genomic DNA. Translation: AAS55971.1. BK006938 Genomic DNA. Translation: DAA11868.1.
PIR	S63429.
RefSeq	NP_010305.1. NM_001180330.1.
3D structure databases
ProteinModelPortal	Q12421.
SMR	Q12421. Positions 2-196.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2667N.
IntAct	Q12421. 6 interactions.
MINT	MINT-536673.
Proteomic databases
PaxDb	Q12421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR022C; YDR022C; YDR022C.
GeneID	851585.
KEGG	sce:YDR022C.
Organism-specific databases
CYGD	YDR022c.
SGD	S000002429. CIS1.
Phylogenomic databases
eggNOG	NOG81313.
HOGENOM	HOG000001031.
OMA	IKYIFED.
OrthoDB	EOG40GH1P.
Gene expression databases
Genevestigator	Q12421.
GermOnline	YDR022C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018621. Autophagy-rel_p31. [Graphical view]
Pfam	PF09795. Atg31. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969058.

Entry information

Entry name

ATG31_YEAST

Accession

Primary (citable) accession number: Q12421
Secondary accession number(s): D6VS08, Q03939

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program