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Protein

Pre-mRNA-splicing factor PRP46

Gene

PRP46

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M (By similarity).By similarity1 Publication

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34048-MONOMER
ReactomeiR-SCE-8951664 Neddylation
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor PRP46
Alternative name(s):
Complexed with CEF1 protein 1
PRP nineteen-associated complex protein 50
PRP19-associated complex protein 50
Pre-mRNA-processing protein 46
Gene namesi
Name:PRP46
Synonyms:CWC1, NTC50
Ordered Locus Names:YPL151C
ORF Names:P2594
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL151C
SGDiS000006072 PRP46

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511691 – 451Pre-mRNA-splicing factor PRP46Add BLAST451

Proteomic databases

MaxQBiQ12417
PaxDbiQ12417
PRIDEiQ12417

2D gel databases

COMPLUYEAST-2DPAGEiQ12417

PTM databases

iPTMnetiQ12417

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CEF1, CLF1, NTC20, PRP45 and SYF1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP45P280044EBI-710,EBI-20640

Protein-protein interaction databases

BioGridi36032, 223 interactors
DIPiDIP-3994N
IntActiQ12417, 45 interactors
MINTiQ12417
STRINGi4932.YPL151C

Structurei

Secondary structure

1451
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi94 – 102Combined sources9
Turni107 – 109Combined sources3
Helixi111 – 119Combined sources9
Beta strandi130 – 135Combined sources6
Beta strandi144 – 147Combined sources4
Beta strandi149 – 151Combined sources3
Beta strandi154 – 158Combined sources5
Beta strandi160 – 168Combined sources9
Turni169 – 171Combined sources3
Beta strandi174 – 179Combined sources6
Beta strandi185 – 190Combined sources6
Beta strandi192 – 201Combined sources10
Beta strandi202 – 204Combined sources3
Beta strandi206 – 210Combined sources5
Turni211 – 214Combined sources4
Beta strandi215 – 220Combined sources6
Beta strandi227 – 232Combined sources6
Beta strandi234 – 236Combined sources3
Beta strandi238 – 243Combined sources6
Beta strandi246 – 255Combined sources10
Beta strandi258 – 262Combined sources5
Beta strandi269 – 274Combined sources6
Beta strandi276 – 279Combined sources4
Beta strandi281 – 285Combined sources5
Beta strandi290 – 293Combined sources4
Turni295 – 297Combined sources3
Beta strandi298 – 304Combined sources7
Beta strandi313 – 316Combined sources4
Turni318 – 320Combined sources3
Beta strandi321 – 326Combined sources6
Beta strandi332 – 335Combined sources4
Turni336 – 338Combined sources3
Beta strandi353 – 358Combined sources6
Beta strandi362 – 368Combined sources7
Beta strandi371 – 380Combined sources10
Beta strandi383 – 388Combined sources6
Helixi397 – 399Combined sources3
Beta strandi402 – 407Combined sources6
Beta strandi409 – 418Combined sources10
Turni419 – 421Combined sources3
Beta strandi422 – 427Combined sources6
Turni434 – 436Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50O1-451[»]
5GMKelectron microscopy3.40O1-451[»]
5LJ3electron microscopy3.80J1-451[»]
5LJ5electron microscopy3.80J1-451[»]
5LQWelectron microscopy5.80K1-451[»]
5MPSelectron microscopy3.85J1-451[»]
5MQ0electron microscopy4.17J1-451[»]
5WSGelectron microscopy4.00O1-451[»]
6BK8electron microscopy3.30D1-451[»]
6EXNelectron microscopy3.70J1-451[»]
ProteinModelPortaliQ12417
SMRiQ12417
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati137 – 168WD 1Add BLAST32
Repeati180 – 210WD 2Add BLAST31
Repeati222 – 252WD 3Add BLAST31
Repeati264 – 294WD 4Add BLAST31
Repeati306 – 335WD 5Add BLAST30
Repeati348 – 377WD 6Add BLAST30
Repeati397 – 427WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00910000144137
HOGENOMiHOG000207595
InParanoidiQ12417
KOiK12862
OMAiFAMCFDQ
OrthoDBiEOG092C2MHF

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 5 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 4 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q12417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR
60 70 80 90 100
YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK
110 120 130 140 150
IFQPEVAEEL IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV
160 170 180 190 200
DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFSV
210 220 230 240 250
SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA TAGRDSVIKL
260 270 280 290 300
WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT
310 320 330 340 350
MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT
360 370 380 390 400
GIINTLSINQ DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER
410 420 430 440 450
SVLCSTFDKT GLRLITGEAD KSIKIWKQDE TATKESEPGL AWNPNLSAKR

F
Length:451
Mass (Da):50,700
Last modified:November 1, 1996 - v1
Checksum:i0E888F566A5414BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA Translation: CAA65570.1
Z73507 Genomic DNA Translation: CAA97856.1
BK006949 Genomic DNA Translation: DAA11284.1
PIRiS65162
RefSeqiNP_015174.1, NM_001183965.1

Genome annotation databases

EnsemblFungiiYPL151C; YPL151C; YPL151C
GeneIDi855952
KEGGisce:YPL151C

Similar proteinsi

Entry informationi

Entry nameiPRP46_YEAST
AccessioniPrimary (citable) accession number: Q12417
Secondary accession number(s): D6W3L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 177 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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