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Protein

Pre-mRNA-splicing factor PRP46

Gene

PRP46

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M (By similarity).By similarity1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor PRP46
Alternative name(s):
Complexed with CEF1 protein 1
PRP nineteen-associated complex protein 50
PRP19-associated complex protein 50
Pre-mRNA-processing protein 46
Gene namesi
Name:PRP46
Synonyms:CWC1, NTC50
Ordered Locus Names:YPL151C
ORF Names:P2594
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL151C.
SGDiS000006072. PRP46.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Prp19 complex Source: SGD
  • spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451Pre-mRNA-splicing factor PRP46PRO_0000051169Add
BLAST

Proteomic databases

MaxQBiQ12417.
PeptideAtlasiQ12417.

2D gel databases

COMPLUYEAST-2DPAGEQ12417.

PTM databases

iPTMnetiQ12417.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CEF1, CLF1, NTC20, PRP45 and SYF1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP45P280046EBI-710,EBI-20640

Protein-protein interaction databases

BioGridi36032. 56 interactions.
DIPiDIP-3994N.
IntActiQ12417. 37 interactions.
MINTiMINT-389419.

Structurei

3D structure databases

ProteinModelPortaliQ12417.
SMRiQ12417. Positions 125-429.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati137 – 16832WD 1Add
BLAST
Repeati180 – 21031WD 2Add
BLAST
Repeati222 – 25231WD 3Add
BLAST
Repeati264 – 29431WD 4Add
BLAST
Repeati306 – 33530WD 5Add
BLAST
Repeati348 – 37730WD 6Add
BLAST
Repeati397 – 42731WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00520000055597.
HOGENOMiHOG000207595.
InParanoidiQ12417.
KOiK12862.
OMAiWRPELLK.
OrthoDBiEOG75XGWC.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR
60 70 80 90 100
YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK
110 120 130 140 150
IFQPEVAEEL IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV
160 170 180 190 200
DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFSV
210 220 230 240 250
SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA TAGRDSVIKL
260 270 280 290 300
WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT
310 320 330 340 350
MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT
360 370 380 390 400
GIINTLSINQ DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER
410 420 430 440 450
SVLCSTFDKT GLRLITGEAD KSIKIWKQDE TATKESEPGL AWNPNLSAKR

F
Length:451
Mass (Da):50,700
Last modified:November 1, 1996 - v1
Checksum:i0E888F566A5414BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65570.1.
Z73507 Genomic DNA. Translation: CAA97856.1.
BK006949 Genomic DNA. Translation: DAA11284.1.
PIRiS65162.
RefSeqiNP_015174.1. NM_001183965.1.

Genome annotation databases

EnsemblFungiiYPL151C; YPL151C; YPL151C.
GeneIDi855952.
KEGGisce:YPL151C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65570.1.
Z73507 Genomic DNA. Translation: CAA97856.1.
BK006949 Genomic DNA. Translation: DAA11284.1.
PIRiS65162.
RefSeqiNP_015174.1. NM_001183965.1.

3D structure databases

ProteinModelPortaliQ12417.
SMRiQ12417. Positions 125-429.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36032. 56 interactions.
DIPiDIP-3994N.
IntActiQ12417. 37 interactions.
MINTiMINT-389419.

PTM databases

iPTMnetiQ12417.

2D gel databases

COMPLUYEAST-2DPAGEQ12417.

Proteomic databases

MaxQBiQ12417.
PeptideAtlasiQ12417.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL151C; YPL151C; YPL151C.
GeneIDi855952.
KEGGisce:YPL151C.

Organism-specific databases

EuPathDBiFungiDB:YPL151C.
SGDiS000006072. PRP46.

Phylogenomic databases

GeneTreeiENSGT00520000055597.
HOGENOMiHOG000207595.
InParanoidiQ12417.
KOiK12862.
OMAiWRPELLK.
OrthoDBiEOG75XGWC.

Enzyme and pathway databases

BioCyciYEAST:G3O-34048-MONOMER.

Miscellaneous databases

PROiQ12417.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators."
    Purnelle B., Coster F., Goffeau A.
    Yeast 12:1483-1492(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis."
    Pardo M., Ward M., Bains S., Molina M., Blackstock W., Gil C., Nombela C.
    Electrophoresis 21:3396-3410(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY.
  5. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "Characterization of interactions among the Cef1p-Prp19p-associated splicing complex."
    Ohi M.D., Gould K.L.
    RNA 8:798-815(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CEF1; CLF1; NTC20 AND SYF1.
  7. "Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors."
    Albers M., Diment A., Muraru M., Russell C.S., Beggs J.D.
    RNA 9:138-150(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRP45.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRP46_YEAST
AccessioniPrimary (citable) accession number: Q12417
Secondary accession number(s): D6W3L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.