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Protein

Pre-mRNA-splicing factor PRP46

Gene

PRP46

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M (By similarity).By similarity1 Publication

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-34048-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor PRP46
Alternative name(s):
Complexed with CEF1 protein 1
PRP nineteen-associated complex protein 50
PRP19-associated complex protein 50
Pre-mRNA-processing protein 46
Gene namesi
Name:PRP46
Synonyms:CWC1, NTC50
Ordered Locus Names:YPL151C
ORF Names:P2594
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL151C.
SGDiS000006072. PRP46.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • Cul4-RING E3 ubiquitin ligase complex Source: GO_Central
  • cytoplasm Source: UniProtKB-SubCell
  • precatalytic spliceosome Source: GO_Central
  • Prp19 complex Source: SGD
  • spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511691 – 451Pre-mRNA-splicing factor PRP46Add BLAST451

Proteomic databases

MaxQBiQ12417.
PRIDEiQ12417.

2D gel databases

COMPLUYEAST-2DPAGEQ12417.

PTM databases

iPTMnetiQ12417.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CEF1, CLF1, NTC20, PRP45 and SYF1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP45P280046EBI-710,EBI-20640

Protein-protein interaction databases

BioGridi36032. 56 interactors.
DIPiDIP-3994N.
IntActiQ12417. 37 interactors.
MINTiMINT-389419.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50O1-451[»]
5GMKelectron microscopy3.40O1-451[»]
5LJ3electron microscopy3.80J1-451[»]
5LJ5electron microscopy3.80J1-451[»]
5LQWelectron microscopy5.80K1-451[»]
ProteinModelPortaliQ12417.
SMRiQ12417.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati137 – 168WD 1Add BLAST32
Repeati180 – 210WD 2Add BLAST31
Repeati222 – 252WD 3Add BLAST31
Repeati264 – 294WD 4Add BLAST31
Repeati306 – 335WD 5Add BLAST30
Repeati348 – 377WD 6Add BLAST30
Repeati397 – 427WD 7Add BLAST31

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

GeneTreeiENSGT00860000133801.
HOGENOMiHOG000207595.
InParanoidiQ12417.
KOiK12862.
OMAiWRPELLK.
OrthoDBiEOG092C2MHF.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12417-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR
60 70 80 90 100
YDTYRKESSS FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK
110 120 130 140 150
IFQPEVAEEL IVNRYEKLLS QRPEWHAPWK LSRVINGHLG WVRCVAIDPV
160 170 180 190 200
DNEWFITGSN DTTMKVWDLA TGKLKTTLAG HVMTVRDVAV SDRHPYLFSV
210 220 230 240 250
SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA TAGRDSVIKL
260 270 280 290 300
WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT
310 320 330 340 350
MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT
360 370 380 390 400
GIINTLSINQ DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER
410 420 430 440 450
SVLCSTFDKT GLRLITGEAD KSIKIWKQDE TATKESEPGL AWNPNLSAKR

F
Length:451
Mass (Da):50,700
Last modified:November 1, 1996 - v1
Checksum:i0E888F566A5414BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65570.1.
Z73507 Genomic DNA. Translation: CAA97856.1.
BK006949 Genomic DNA. Translation: DAA11284.1.
PIRiS65162.
RefSeqiNP_015174.1. NM_001183965.1.

Genome annotation databases

EnsemblFungiiYPL151C; YPL151C; YPL151C.
GeneIDi855952.
KEGGisce:YPL151C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96770 Genomic DNA. Translation: CAA65570.1.
Z73507 Genomic DNA. Translation: CAA97856.1.
BK006949 Genomic DNA. Translation: DAA11284.1.
PIRiS65162.
RefSeqiNP_015174.1. NM_001183965.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50O1-451[»]
5GMKelectron microscopy3.40O1-451[»]
5LJ3electron microscopy3.80J1-451[»]
5LJ5electron microscopy3.80J1-451[»]
5LQWelectron microscopy5.80K1-451[»]
ProteinModelPortaliQ12417.
SMRiQ12417.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36032. 56 interactors.
DIPiDIP-3994N.
IntActiQ12417. 37 interactors.
MINTiMINT-389419.

PTM databases

iPTMnetiQ12417.

2D gel databases

COMPLUYEAST-2DPAGEQ12417.

Proteomic databases

MaxQBiQ12417.
PRIDEiQ12417.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL151C; YPL151C; YPL151C.
GeneIDi855952.
KEGGisce:YPL151C.

Organism-specific databases

EuPathDBiFungiDB:YPL151C.
SGDiS000006072. PRP46.

Phylogenomic databases

GeneTreeiENSGT00860000133801.
HOGENOMiHOG000207595.
InParanoidiQ12417.
KOiK12862.
OMAiWRPELLK.
OrthoDBiEOG092C2MHF.

Enzyme and pathway databases

BioCyciYEAST:G3O-34048-MONOMER.

Miscellaneous databases

PROiQ12417.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP46_YEAST
AccessioniPrimary (citable) accession number: Q12417
Secondary accession number(s): D6W3L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.