Q12417 (PRP46_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-mRNA-splicing factor PRP46

Alternative name(s):
Complexed with CEF1 protein 1
PRP nineteen-associated complex protein 50
PRP19-associated complex protein 50
Pre-mRNA-processing protein 46

Gene names

Name:	PRP46
Synonyms:	CWC1, NTC50
Ordered Locus Names:	YPL151C
ORF Names:	P2594

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. May also be required for cell cycle progression at G2/M By similarity. Ref.7
Subunit structure	Belongs to the CWC complex (or PRP19-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2. Interacts with CEF1, CLF1, NTC20, PRP45 and SYF1. Ref.6 Ref.7
Subcellular location	Cytoplasm. Nucleus Ref.8.
Miscellaneous	Present with 1770 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the WD repeat PRL1/PRL2 family. Contains 7 WD repeats.

Ontologies

Keywords
Biological process	Cell cycle mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus Spliceosome
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW nuclear mRNA splicing, via spliceosome Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell spliceosomal complex Inferred from direct assay Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCT6	P39079	2	EBI-710,EBI-19077
PRP45	P28004	6	EBI-710,EBI-20640

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Pre-mRNA-splicing factor PRP46		PRO_0000051169
Regions
	Repeat	137 – 168	32	WD 1
	Repeat	180 – 210	31	WD 2
	Repeat	222 – 252	31	WD 3
	Repeat	264 – 294	31	WD 4
	Repeat	306 – 335	30	WD 5
	Repeat	348 – 377	30	WD 6
	Repeat	397 – 427	31	WD 7
Amino acid modifications
	Modified residue	58	1	Phosphoserine Ref.10
	Modified residue	60	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	Q12417 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0E888F566A5414BD	FASTA	451	50,700
	10 20 30 40 50 60 MDGNDHKVEN LGDVDKFYSR IRWNNQFSYM ATLPPHLQSE MEGQKSLLMR YDTYRKESSS 70 80 90 100 110 120 FSGEGKKVTL QHVPTDFSEA SQAVISKKDH DTHASAFVNK IFQPEVAEEL IVNRYEKLLS 130 140 150 160 170 180 QRPEWHAPWK LSRVINGHLG WVRCVAIDPV DNEWFITGSN DTTMKVWDLA TGKLKTTLAG 190 200 210 220 230 240 HVMTVRDVAV SDRHPYLFSV SEDKTVKCWD LEKNQIIRDY YGHLSGVRTV SIHPTLDLIA 250 260 270 280 290 300 TAGRDSVIKL WDMRTRIPVI TLVGHKGPIN QVQCTPVDPQ VVSSSTDATV RLWDVVAGKT 310 320 330 340 350 360 MKVLTHHKRS VRATALHPKE FSVASACTDD IRSWGLAEGS LLTNFESEKT GIINTLSINQ 370 380 390 400 410 420 DDVLFAGGDN GVLSFYDYKS GHKYQSLATR EMVGSLEGER SVLCSTFDKT GLRLITGEAD 430 440 450 KSIKIWKQDE TATKESEPGL AWNPNLSAKR F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"A proteomic approach for the study of Saccharomyces cerevisiae cell wall biogenesis." Pardo M., Ward M., Bains S., Molina M., Blackstock W., Gil C., Nombela C. Electrophoresis 21:3396-3410(2000) [PubMed: 11079560] [Abstract] Cited for: MASS SPECTROMETRY, PARTIAL PROTEIN SEQUENCE.
[5]	"Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs." Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L. Mol. Cell. Biol. 22:2011-2024(2002) [PubMed: 11884590] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE CWC COMPLEX.
[6]	"Characterization of interactions among the Cef1p-Prp19p-associated splicing complex." Ohi M.D., Gould K.L. RNA 8:798-815(2002) [PubMed: 12088152] [Abstract] Cited for: INTERACTION WITH CEF1; CLF1; NTC20 AND SYF1.
[7]	"Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors." Albers M., Diment A., Muraru M., Russell C.S., Beggs J.D. RNA 9:138-150(2003) [PubMed: 12554883] [Abstract] Cited for: FUNCTION, INTERACTION WITH PRP45.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-58 AND SER-60, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96770 Genomic DNA. Translation: CAA65570.1. Z73507 Genomic DNA. Translation: CAA97856.1. BK006949 Genomic DNA. Translation: DAA11284.1.
PIR	S65162.
RefSeq	NP_015174.1. NM_001183965.1.
3D structure databases
ProteinModelPortal	Q12417.
SMR	Q12417. Positions 93-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3994N.
IntAct	Q12417. 41 interactions.
MINT	MINT-389419.
STRING	Q12417.
2D gel databases
COMPLUYEAST-2DPAGE	Q12417.
Proteomic databases
PeptideAtlas	Q12417.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL151C; YPL151C; YPL151C.
GeneID	855952.
KEGG	sce:YPL151C.
NMPDR	fig|4932.3.peg.6304.
Organism-specific databases
CYGD	YPL151c.
SGD	S000006072. PRP46.
Phylogenomic databases
eggNOG	fuNOG05568.
GeneTree	EFGT00050000000044.
HOGENOM	HBG630928.
OMA	ITAEADK.
OrthoDB	EOG4QZBVP.
Gene expression databases
ArrayExpress	Q12417.
Genevestigator	Q12417.
GermOnline	YPL151C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K12862.
Pfam	PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 4 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980735.

Entry information

Entry name

PRP46_YEAST

Accession

Primary (citable) accession number: Q12417
Secondary accession number(s): D6W3L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program