Q12415 (TFC7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor tau 55 kDa subunit

Alternative name(s):
TFIIIC 55 kDa subunit
Transcription factor C subunit 7

Gene names

Name:	TFC7
Ordered Locus Names:	YOR110W
ORF Names:	YOR3234w, O3234

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	435 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauA domain of TFIIIC that binds boxA DNA promoter sites of tRNA and similar genes. Ref.1
Subunit structure	Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker.
Subcellular location	Nucleus Ref.7.
Miscellaneous	Present with 2660 molecules/cell in log phase SD medium. Ref.8

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase III promoter Traceable author statement. Source: SGD
Cellular component	transcription factor TFIIIC complex Traceable author statement. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 435	435	Transcription factor tau 55 kDa subunit		PRO_0000072499
Amino acid modifications
	Modified residue	224	1	Phosphothreonine Ref.12
	Modified residue	225	1	Phosphoserine Ref.11 Ref.12
	Modified residue	279	1	Phosphoserine Ref.12
	Modified residue	280	1	Phosphoserine Ref.12
	Modified residue	365	1	Phosphoserine Ref.9 Ref.10 Ref.12
	Modified residue	367	1	Phosphothreonine Ref.12
Experimental info
	Sequence conflict	9	1	A → V in AAT92929. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q12415 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D778B81F31B83C45	FASTA	435	49,146
	10 20 30 40 50 60 MVVNTIYIAR HGYRSNWLPE GPYPDPLTGI DSDVPLAEHG VQQAKELAHY LLSLDNQPEA 70 80 90 100 110 120 AFASPFYRCL ETVQPIAKLL EIPVYLERGI GEWYRPDRKP VIPVPAGYEI LSKFFPGVIS 130 140 150 160 170 180 QEWDSTLTPN EKGETEQEMY MRFKKFWPLF IERVEKEYPN VECILLVTHA ASKIALGMSL 190 200 210 220 230 240 LGYDNPRMSL NENGDKIRSG SCSLDKYEIL KKSYDTIDET DDQTSFTYIP FSDRKWVLTM 250 260 270 280 290 300 NGNTEFLSSG EEMNWNFDCV AEAGSDADIK KRQMTKKTSS PIPEADDQTE VETVYISVDI 310 320 330 340 350 360 PSGNYKERTE IAKSAILQYS GLETDAPLFR IGNRLYEGSW ERLVGTELAF PNAAHVHKKT 370 380 390 400 410 420 AGLLSPTEEN ETTNAGQSKG SSTANDPNIQ IQEEDVGLPD STNTSRDHTG DKEEVQSEKI 430 YRIKERIVLS NVRPM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A chimeric subunit of yeast transcription factor IIIC forms a subcomplex with tau95." Manaud N., Arrebola R., Buffin-Meyer B., Lefebvre O., Voss H., Riva M., Conesa C., Sentenac A. Mol. Cell. Biol. 18:3191-3200(1998) [PubMed: 9584160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 62-66; 81-85; 183-188; 227-237; 341-346 AND 347-352, FUNCTION, IDENTIFICATION IN TFIIIC.
[2]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed: 9200815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames." Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W. Yeast 12:281-288(1996) [PubMed: 8904341] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 222-435.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-365, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-224; SER-225; SER-279; SER-280; SER-365 AND THR-367, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94335 Genomic DNA. Translation: CAA64030.1. Z75018 Genomic DNA. Translation: CAA99308.1. AY692910 Genomic DNA. Translation: AAT92929.1. X90518 Genomic DNA. Translation: CAA62104.1. BK006948 Genomic DNA. Translation: DAA10885.1.
PIR	S61668.
RefSeq	NP_014753.1. NM_001183529.1.
3D structure databases
ProteinModelPortal	Q12415.
SMR	Q12415. Positions 4-212.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2309N.
IntAct	Q12415. 17 interactions.
MINT	MINT-512728.
STRING	Q12415.
Proteomic databases
PeptideAtlas	Q12415.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR110W; YOR110W; YOR110W.
GeneID	854277.
KEGG	sce:YOR110W.
NMPDR	fig|4932.3.peg.5856.
Organism-specific databases
CYGD	YOR110w.
SGD	S000005636. TFC7.
Phylogenomic databases
eggNOG	fuNOG04484.
GeneTree	EFGT00050000004868.
HOGENOM	HBG756784.
OMA	GACSLDK.
OrthoDB	EOG4T7CC9.
Gene expression databases
ArrayExpress	Q12415.
Genevestigator	Q12415.
GermOnline	YOR110W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013078. His_Pase_superF_clade-1. IPR014623. Tau55_TFC7. IPR019481. TFIIIC_tau55-rel. [Graphical view]
KO	K15206.
Pfam	PF00300. His_Phos_1. 1 hit. PF10419. TFIIIC_sub6. 1 hit. [Graphical view]
PIRSF	PIRSF036802. Tau55_TFC7. 1 hit.
SMART	SM00855. PGAM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976239.

Entry information

Entry name

TFC7_YEAST

Accession

Primary (citable) accession number: Q12415
Secondary accession number(s): D6W2G9, Q07347, Q6B220

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program