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Protein

Actin-related protein 7

Gene

ARP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in transcriptional regulation. Heterodimer of ARP7 and ARP9 functions with HMG box proteins to facilitate proper chromatin architecture. Heterodimer formation is necessary for assembly into RSC complex. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.8 Publications

GO - Molecular functioni

  • structural molecule activity Source: UniProtKB

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: UniProtKB
  • chromatin modification Source: UniProtKB-KW
  • chromatin organization Source: UniProtKB
  • nucleosome disassembly Source: SGD
  • nucleosome mobilization Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB
  • transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34193-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 7
Alternative name(s):
Actin-like protein ARP7
Chromatin structure-remodeling complex protein ARP7
SWI/SNF complex component ARP7
Gene namesi
Name:ARP7
Synonyms:SWP61
Ordered Locus Names:YPR034W
ORF Names:YP9367.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR034W.
SGDiS000006238. ARP7.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  • nucleus Source: SGD
  • RSC complex Source: UniProtKB
  • SWI/SNF complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi19A → P: Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 2 Publications1
Mutagenesisi33S → F: Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 2 Publications1
Mutagenesisi396G → V: Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 1 Publication1
Mutagenesisi411E → K: Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000891221 – 477Actin-related protein 7Add BLAST477

Proteomic databases

MaxQBiQ12406.
PRIDEiQ12406.

PTM databases

iPTMnetiQ12406.

Interactioni

Subunit structurei

Forms a heterodimer with ARP9. Interacts with NPL6. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP9Q051235EBI-2962,EBI-2972

Protein-protein interaction databases

BioGridi36212. 58 interactors.
DIPiDIP-6351N.
IntActiQ12406. 56 interactors.
MINTiMINT-686249.

Structurei

Secondary structure

1477
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 12Combined sources5
Beta strandi14 – 21Combined sources8
Beta strandi24 – 26Combined sources3
Beta strandi28 – 37Combined sources10
Beta strandi41 – 43Combined sources3
Beta strandi45 – 48Combined sources4
Helixi51 – 59Combined sources9
Beta strandi66 – 71Combined sources6
Beta strandi75 – 77Combined sources3
Helixi80 – 93Combined sources14
Turni99 – 101Combined sources3
Beta strandi104 – 108Combined sources5
Beta strandi112 – 114Combined sources3
Helixi116 – 127Combined sources12
Turni128 – 131Combined sources4
Beta strandi134 – 140Combined sources7
Helixi141 – 147Combined sources7
Turni148 – 150Combined sources3
Beta strandi152 – 159Combined sources8
Beta strandi164 – 170Combined sources7
Helixi176 – 178Combined sources3
Helixi186 – 197Combined sources12
Helixi198 – 200Combined sources3
Helixi217 – 225Combined sources9
Helixi227 – 234Combined sources8
Helixi243 – 261Combined sources19
Helixi283 – 285Combined sources3
Beta strandi287 – 292Combined sources6
Helixi293 – 295Combined sources3
Beta strandi297 – 302Combined sources6
Helixi303 – 314Combined sources12
Helixi316 – 318Combined sources3
Helixi325 – 327Combined sources3
Helixi329 – 338Combined sources10
Turni339 – 342Combined sources4
Helixi344 – 347Combined sources4
Helixi381 – 388Combined sources8
Beta strandi391 – 396Combined sources6
Helixi397 – 400Combined sources4
Helixi404 – 415Combined sources12
Beta strandi423 – 425Combined sources3
Helixi429 – 433Combined sources5
Helixi435 – 444Combined sources10
Helixi449 – 451Combined sources3
Beta strandi454 – 456Combined sources3
Helixi457 – 463Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WEEX-ray3.10B1-477[»]
4I6MX-ray2.80A1-477[»]
ProteinModelPortaliQ12406.
SMRiQ12406.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000132076.
HOGENOMiHOG000034072.
InParanoidiQ12406.
KOiK11767.
OMAiIRFPQYK.
OrthoDBiEOG092C2NCX.

Family and domain databases

InterProiIPR004000. Actin.
IPR029911. Arp7_fungi.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 4 hits.
PTHR11937:SF233. PTHR11937:SF233. 4 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12406-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNRKCVVI HNGSHRTVAG FSNVELPQCI IPSSYIKRTD EGGEAEFIFG
60 70 80 90 100
TYNMIDAAAE KRNGDEVYTL VDSQGLPYNW DALEMQWRYL YDTQLKVSPE
110 120 130 140 150
ELPLVITMPA TNGKPDMAIL ERYYELAFDK LNVPVFQIVI EPLAIALSMG
160 170 180 190 200
KSSAFVIDIG ASGCNVTPII DGIVVKNAVV RSKFGGDFLD FQVHERLAPL
210 220 230 240 250
IKEENDMENM ADEQKRSTDV WYEASTWIQQ FKSTMLQVSE KDLFELERYY
260 270 280 290 300
KEQADIYAKQ QEQLKQMDQQ LQYTALTGSP NNPLVQKKNF LFKPLNKTLT
310 320 330 340 350
LDLKECYQFA EYLFKPQLIS DKFSPEDGLG PLMAKSVKKA GASINSMKAN
360 370 380 390 400
TSTNPNGLGT SHINTNVGDN NSTASSSNIS PEQVYSLLLT NVIITGSTSL
410 420 430 440 450
IEGMEQRIIK ELSIRFPQYK LTTFANQVMM DRKIQGWLGA LTMANLPSWS
460 470
LGKWYSKEDY ETLKRDRKQS QATNATN
Length:477
Mass (Da):53,810
Last modified:November 1, 1996 - v1
Checksum:i8E105921576FA213
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94984.1.
Z49274 Genomic DNA. Translation: CAA89288.1.
BK006949 Genomic DNA. Translation: DAA11460.1.
PIRiS54508.
RefSeqiNP_015359.1. NM_001184131.1.

Genome annotation databases

EnsemblFungiiYPR034W; YPR034W; YPR034W.
GeneIDi856146.
KEGGisce:YPR034W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94984.1.
Z49274 Genomic DNA. Translation: CAA89288.1.
BK006949 Genomic DNA. Translation: DAA11460.1.
PIRiS54508.
RefSeqiNP_015359.1. NM_001184131.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WEEX-ray3.10B1-477[»]
4I6MX-ray2.80A1-477[»]
ProteinModelPortaliQ12406.
SMRiQ12406.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36212. 58 interactors.
DIPiDIP-6351N.
IntActiQ12406. 56 interactors.
MINTiMINT-686249.

PTM databases

iPTMnetiQ12406.

Proteomic databases

MaxQBiQ12406.
PRIDEiQ12406.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR034W; YPR034W; YPR034W.
GeneIDi856146.
KEGGisce:YPR034W.

Organism-specific databases

EuPathDBiFungiDB:YPR034W.
SGDiS000006238. ARP7.

Phylogenomic databases

GeneTreeiENSGT00840000132076.
HOGENOMiHOG000034072.
InParanoidiQ12406.
KOiK11767.
OMAiIRFPQYK.
OrthoDBiEOG092C2NCX.

Enzyme and pathway databases

BioCyciYEAST:G3O-34193-MONOMER.

Miscellaneous databases

PROiQ12406.

Family and domain databases

InterProiIPR004000. Actin.
IPR029911. Arp7_fungi.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 4 hits.
PTHR11937:SF233. PTHR11937:SF233. 4 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARP7_YEAST
AccessioniPrimary (citable) accession number: Q12406
Secondary accession number(s): D6W444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.