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Q12406

- ARP7_YEAST

UniProt

Q12406 - ARP7_YEAST

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Protein

Actin-related protein 7

Gene

ARP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of the chromatin structure remodeling complex (RSC), which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. This subunit is involved in transcriptional regulation. Heterodimer of ARP7 and ARP9 functions with HMG box proteins to facilitate proper chromatin architecture. Heterodimer formation is necessary for assembly into RSC complex. Part of the SWI/SNF complex, an ATP-dependent chromatin remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.8 Publications

GO - Molecular functioni

  1. structural molecule activity Source: UniProtKB

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP-dependent chromatin remodeling Source: UniProtKB
  3. chromatin organization Source: UniProtKB
  4. nucleosome disassembly Source: SGD
  5. nucleosome mobilization Source: SGD
  6. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  7. regulation of transcription, DNA-templated Source: UniProtKB
  8. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-34193-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 7
Alternative name(s):
Actin-like protein ARP7
Chromatin structure-remodeling complex protein ARP7
SWI/SNF complex component ARP7
Gene namesi
Name:ARP7
Synonyms:SWP61
Ordered Locus Names:YPR034W
ORF Names:YP9367.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR034w.
SGDiS000006238. ARP7.

Subcellular locationi

Nucleus 1 Publication
Note: Localizes to centromeric and flanking chromatin. Association with these loci is dependent on STH1.

GO - Cellular componenti

  1. nucleus Source: SGD
  2. RSC complex Source: UniProtKB
  3. SWI/SNF complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191A → P: Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 2 Publications
Mutagenesisi33 – 331S → F: Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 2 Publications
Mutagenesisi396 – 3961G → V: Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 1 Publication
Mutagenesisi411 – 4111E → K: Impaired heterodimerization with ARP9. Temperature-sensitive phenotype. Moderate suppressor of Ty phenotype. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Actin-related protein 7PRO_0000089122Add
BLAST

Proteomic databases

MaxQBiQ12406.
PaxDbiQ12406.
PeptideAtlasiQ12406.

Expressioni

Gene expression databases

GenevestigatoriQ12406.

Interactioni

Subunit structurei

Forms a heterodimer with ARP9. Interacts with NPL6. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARP9Q051235EBI-2962,EBI-2972

Protein-protein interaction databases

BioGridi36212. 60 interactions.
DIPiDIP-6351N.
IntActiQ12406. 55 interactions.
MINTiMINT-686249.
STRINGi4932.YPR034W.

Structurei

Secondary structure

1
477
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 125
Beta strandi14 – 218
Beta strandi24 – 263
Beta strandi28 – 3710
Beta strandi41 – 433
Beta strandi45 – 484
Helixi51 – 599
Beta strandi66 – 716
Beta strandi75 – 773
Helixi80 – 9314
Turni99 – 1013
Beta strandi104 – 1085
Beta strandi112 – 1143
Helixi116 – 12712
Turni128 – 1314
Beta strandi134 – 1407
Helixi141 – 1477
Turni148 – 1503
Beta strandi152 – 1598
Beta strandi164 – 1707
Helixi176 – 1783
Helixi186 – 19712
Helixi198 – 2003
Helixi217 – 2259
Helixi227 – 2348
Helixi243 – 26119
Helixi283 – 2853
Beta strandi287 – 2926
Helixi293 – 2953
Beta strandi297 – 3026
Helixi303 – 31412
Helixi316 – 3183
Helixi325 – 3273
Helixi329 – 33810
Turni339 – 3424
Helixi344 – 3474
Helixi381 – 3888
Beta strandi391 – 3966
Helixi397 – 4004
Helixi404 – 41512
Beta strandi423 – 4253
Helixi429 – 4335
Helixi435 – 44410
Helixi449 – 4513
Beta strandi454 – 4563
Helixi457 – 4637

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WEEX-ray3.10B1-477[»]
4I6MX-ray2.80A1-477[»]
ProteinModelPortaliQ12406.
SMRiQ12406. Positions 4-468.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiCOG5277.
HOGENOMiHOG000034072.
InParanoidiQ12406.
KOiK11767.
OMAiGMEQRII.
OrthoDBiEOG7X6M98.

Family and domain databases

InterProiIPR004000. Actin-related.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12406-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTLNRKCVVI HNGSHRTVAG FSNVELPQCI IPSSYIKRTD EGGEAEFIFG
60 70 80 90 100
TYNMIDAAAE KRNGDEVYTL VDSQGLPYNW DALEMQWRYL YDTQLKVSPE
110 120 130 140 150
ELPLVITMPA TNGKPDMAIL ERYYELAFDK LNVPVFQIVI EPLAIALSMG
160 170 180 190 200
KSSAFVIDIG ASGCNVTPII DGIVVKNAVV RSKFGGDFLD FQVHERLAPL
210 220 230 240 250
IKEENDMENM ADEQKRSTDV WYEASTWIQQ FKSTMLQVSE KDLFELERYY
260 270 280 290 300
KEQADIYAKQ QEQLKQMDQQ LQYTALTGSP NNPLVQKKNF LFKPLNKTLT
310 320 330 340 350
LDLKECYQFA EYLFKPQLIS DKFSPEDGLG PLMAKSVKKA GASINSMKAN
360 370 380 390 400
TSTNPNGLGT SHINTNVGDN NSTASSSNIS PEQVYSLLLT NVIITGSTSL
410 420 430 440 450
IEGMEQRIIK ELSIRFPQYK LTTFANQVMM DRKIQGWLGA LTMANLPSWS
460 470
LGKWYSKEDY ETLKRDRKQS QATNATN
Length:477
Mass (Da):53,810
Last modified:November 1, 1996 - v1
Checksum:i8E105921576FA213
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71255 Genomic DNA. Translation: CAA94984.1.
Z49274 Genomic DNA. Translation: CAA89288.1.
BK006949 Genomic DNA. Translation: DAA11460.1.
PIRiS54508.
RefSeqiNP_015359.1. NM_001184131.1.

Genome annotation databases

EnsemblFungiiYPR034W; YPR034W; YPR034W.
GeneIDi856146.
KEGGisce:YPR034W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71255 Genomic DNA. Translation: CAA94984.1 .
Z49274 Genomic DNA. Translation: CAA89288.1 .
BK006949 Genomic DNA. Translation: DAA11460.1 .
PIRi S54508.
RefSeqi NP_015359.1. NM_001184131.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3WEE X-ray 3.10 B 1-477 [» ]
4I6M X-ray 2.80 A 1-477 [» ]
ProteinModelPortali Q12406.
SMRi Q12406. Positions 4-468.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36212. 60 interactions.
DIPi DIP-6351N.
IntActi Q12406. 55 interactions.
MINTi MINT-686249.
STRINGi 4932.YPR034W.

Proteomic databases

MaxQBi Q12406.
PaxDbi Q12406.
PeptideAtlasi Q12406.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR034W ; YPR034W ; YPR034W .
GeneIDi 856146.
KEGGi sce:YPR034W.

Organism-specific databases

CYGDi YPR034w.
SGDi S000006238. ARP7.

Phylogenomic databases

eggNOGi COG5277.
HOGENOMi HOG000034072.
InParanoidi Q12406.
KOi K11767.
OMAi GMEQRII.
OrthoDBi EOG7X6M98.

Enzyme and pathway databases

BioCyci YEAST:G3O-34193-MONOMER.

Miscellaneous databases

NextBioi 981268.

Gene expression databases

Genevestigatori Q12406.

Family and domain databases

InterProi IPR004000. Actin-related.
[Graphical view ]
PANTHERi PTHR11937. PTHR11937. 1 hit.
Pfami PF00022. Actin. 1 hit.
[Graphical view ]
SMARTi SM00268. ACTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF."
    Cairns B.R., Erdjument-Bromage H., Tempst P., Winston F., Kornberg R.D.
    Mol. Cell 2:639-651(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 184-196; 217-232 AND 289-297, FUNCTION, IDENTIFICATION IN THE RSC AND SWI/SNF COMPLEXES, MUTAGENESIS OF ALA-19; SER-33; GLY-396 AND GLU-411.
  4. Cited for: FUNCTION OF THE RSC COMPLEX, COMPOSITION OF THE RSC COMPLEX.
  5. "Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family."
    Poch O., Winsor B.
    Yeast 13:1053-1058(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  6. "Histone octamer transfer by a chromatin-remodeling complex."
    Lorch Y., Zhang M., Kornberg R.D.
    Cell 96:389-392(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  7. "Transcriptional repression of the yeast CHA1 gene requires the chromatin-remodeling complex RSC."
    Moreira J.M.A., Holmberg S.
    EMBO J. 18:2836-2844(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  8. "Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains."
    Cairns B.R., Schlichter A., Erdjument-Bromage H., Tempst P., Kornberg R.D., Winston F.
    Mol. Cell 4:715-723(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPOSITION OF THE RSC COMPLEX.
  9. "Chromatin remodeling by RSC involves ATP-dependent DNA translocation."
    Saha A., Wittmeyer J., Cairns B.R.
    Genes Dev. 16:2120-2134(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  10. "Yeast RSC function is required for organization of the cellular cytoskeleton via an alternative PKC1 pathway."
    Chai B., Hsu J.-M., Du J., Laurent B.C.
    Genetics 161:575-584(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX.
  11. "The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling."
    Szerlong H., Saha A., Cairns B.R.
    EMBO J. 22:3175-3187(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HETERODIMERIC COMPLEX FORMATION WITH ARP9 WITHIN THE RSC COMPLEX, MUTAGENESIS OF ALA-19; SER-33 AND GLU-411.
  12. "The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation."
    Hsu J.-M., Huang J., Meluh P.B., Laurent B.C.
    Mol. Cell. Biol. 23:3202-3215(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE RSC COMPLEX, SUBCELLULAR LOCATION, INTERACTION OF THE RSC COMPLEX WITH HISTONES.
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Structural analysis of the yeast SWI/SNF chromatin remodeling complex."
    Smith C.L., Horowitz-Scherer R., Flanagan J.F., Woodcock C.L., Peterson C.L.
    Nat. Struct. Biol. 10:141-145(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF THE SWI/SNF COMPLEX, ELECTRON MICROSCOPY OF THE SWI/SNF COMPLEX.
  15. "The RSC chromatin remodeling complex bears an essential fungal-specific protein module with broad functional roles."
    Wilson B., Erdjument-Bromage H., Tempst P., Cairns B.R.
    Genetics 172:795-809(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NPL6.

Entry informationi

Entry nameiARP7_YEAST
AccessioniPrimary (citable) accession number: Q12406
Secondary accession number(s): D6W444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3