Reviewed, UniProtKB/Swiss-Prot Q12404 (MPD1_YEAST)

Names and origin

Protein names

Recommended name:
    Protein disulfide-isomerase MPD1
    EC=5.3.4.1

Gene names

Name:	MPD1
Ordered Locus Names:	YOR288C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in the folding of proteins containing disulfide bonds.
Catalytic activity	Catalyzes the rearrangement of -S-S- bonds in proteins.
Subcellular location	Endoplasmic reticulum lumen Potential.
Miscellaneous	Present with 830 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Redox-active center Signal
Molecular function	Isomerase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein folding Inferred from genetic interaction. Source: SGD
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole Inferred from direct assay. Source: SGD
Molecular function	protein disulfide isomerase activity Inferred from direct assay. Source: SGD protein disulfide oxidoreductase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 318	297	Protein disulfide-isomerase MPD1		PRO_0000034220
Regions
	Domain	22 – 158	137	Thioredoxin
	Motif	315 – 318	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	47	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Disulfide bond	59 ↔ 62		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12404-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3F96477E8182DC5C	FASTA	318	36,408
	10 20 30 40 50 60 MLFLNIIKLL LGLFIMNEVK AQNFYDSDPH ISELTPKSFD KAIHNTNYTS LVEFYAPWCG 70 80 90 100 110 120 HCKKLSSTFR KAAKRLDGVV QVAAVNCDLN KNKALCAKYD VNGFPTLMVF RPPKIDLSKP 130 140 150 160 170 180 IDNAKKSFSA HANEVYSGAR TLAPIVDFSL SRIRSYVKKF VRIDTLGSLL RKSPKLSVVL 190 200 210 220 230 240 FSKQDKISPV YKSIALDWLG KFDFYSISNK KLKQLTDMNP TYEKTPEIFK YLQKVIPEQR 250 260 270 280 290 300 QSDKSKLVVF DADKDKFWEY EGNSINKNDI SKFLRDTFSI TPNEGPFSRR SEYIAYLKTG 310 KKPIKKNHSS SGNKHDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion." Tachikawa H., Takeuchi Y., Funahashi W., Miura T., Gao X.D., Fujimoto D., Mizunaga T., Onodera K. FEBS Lett. 369:212-216(1995) [PubMed: 7649260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae." Cheret G., Bernardi A., Sor F.J. Yeast 12:1059-1064(1996) [PubMed: 8896271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D34633 Genomic DNA. Translation: BAA07015.1.
X89633 Genomic DNA. Translation: CAA61791.1.
Z75196 Genomic DNA. Translation: CAA99515.1.

PIR

S67190.

RefSeq

NP_014931.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ED3	X-ray	2.00	A/B	23-310	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4085N.

IntAct

Q12404. 1 interaction.

Proteomic databases

PeptideAtlas

Q12404.

Genome annotation databases

Ensembl

YOR288C. Saccharomyces cerevisiae. [Contig view]

GeneID

854462.

GenomeReviews

Gene locus YOR288C in contig Y13140_GR.

KEGG

sce:YOR288C.

NMPDR

fig|4932.3.peg.6043.

Organism-specific databases

CYGD

YOR288c.

SGD

S000005814. MPD1.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q12404.

OMA

Q12404. TTLVEFY.

Enzyme and pathway databases

BRENDA

5.3.4.1. 250.

Gene expression databases

GermOnline

YOR288C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000886. ER_targeting_sequence.
IPR017936. Thioredoxin-like.
IPR006662. Thioredoxin-like_subdom.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF00085. Thioredoxin. 1 hit.
[Graphical view]

PRINTS

PR00421. THIOREDOXIN.

PROSITE

PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

976742.

Entry information

Entry name

MPD1_YEAST

Accession

Primary (citable) accession number: Q12404

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)