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Q12404

- MPD1_YEAST

UniProt

Q12404 - MPD1_YEAST

Protein

Protein disulfide-isomerase MPD1

Gene

MPD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Participates in the folding of proteins containing disulfide bonds.1 Publication

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    GO - Molecular functioni

    1. protein binding Source: SGD
    2. protein disulfide isomerase activity Source: SGD
    3. protein disulfide oxidoreductase activity Source: SGD
    4. protein-disulfide reductase (glutathione) activity Source: SGD

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. oxidation-reduction process Source: GOC
    3. protein folding Source: SGD

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciYEAST:YOR288C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase MPD1 (EC:5.3.4.1)
    Gene namesi
    Name:MPD1
    Ordered Locus Names:YOR288C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR288c.
    SGDiS000005814. MPD1.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 318297Protein disulfide-isomerase MPD1PRO_0000034220Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi59 ↔ 62Redox-activePROSITE-ProRule annotation
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ12404.
    PaxDbiQ12404.
    PeptideAtlasiQ12404.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12404.

    Interactioni

    Subunit structurei

    Interacts with CNE1 and EPS1.1 Publication

    Protein-protein interaction databases

    BioGridi34675. 25 interactions.
    DIPiDIP-4085N.
    IntActiQ12404. 3 interactions.
    MINTiMINT-479800.
    STRINGi4932.YOR288C.

    Structurei

    Secondary structure

    1
    318
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi36 – 438
    Beta strandi45 – 484
    Beta strandi50 – 556
    Helixi60 – 634
    Helixi66 – 7510
    Turni76 – 794
    Beta strandi80 – 867
    Turni90 – 923
    Helixi93 – 986
    Beta strandi103 – 1119
    Beta strandi132 – 1354
    Helixi142 – 1509
    Beta strandi157 – 1593
    Helixi163 – 1653
    Helixi166 – 1705
    Beta strandi174 – 18613
    Helixi189 – 1979
    Beta strandi202 – 2087
    Helixi209 – 2113
    Helixi226 – 23914
    Beta strandi246 – 2516
    Turni252 – 2554
    Beta strandi256 – 2594
    Helixi267 – 27812
    Beta strandi283 – 2853
    Helixi289 – 29911

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ED3X-ray2.00A/B23-310[»]
    ProteinModelPortaliQ12404.
    SMRiQ12404. Positions 22-301.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12404.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 158137ThioredoxinPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi315 – 3184Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 1 thioredoxin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Signal

    Phylogenomic databases

    eggNOGiCOG0526.
    GeneTreeiENSGT00730000110455.
    HOGENOMiHOG000248396.
    KOiK09584.
    OMAiRDENIME.
    OrthoDBiEOG71CFZN.

    Family and domain databases

    Gene3Di3.40.30.10. 1 hit.
    InterProiIPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 1 hit.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 1 hit.
    PS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q12404-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLFLNIIKLL LGLFIMNEVK AQNFYDSDPH ISELTPKSFD KAIHNTNYTS    50
    LVEFYAPWCG HCKKLSSTFR KAAKRLDGVV QVAAVNCDLN KNKALCAKYD 100
    VNGFPTLMVF RPPKIDLSKP IDNAKKSFSA HANEVYSGAR TLAPIVDFSL 150
    SRIRSYVKKF VRIDTLGSLL RKSPKLSVVL FSKQDKISPV YKSIALDWLG 200
    KFDFYSISNK KLKQLTDMNP TYEKTPEIFK YLQKVIPEQR QSDKSKLVVF 250
    DADKDKFWEY EGNSINKNDI SKFLRDTFSI TPNEGPFSRR SEYIAYLKTG 300
    KKPIKKNHSS SGNKHDEL 318
    Length:318
    Mass (Da):36,408
    Last modified:November 1, 1996 - v1
    Checksum:i3F96477E8182DC5C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D34633 Genomic DNA. Translation: BAA07015.1.
    X89633 Genomic DNA. Translation: CAA61791.1.
    Z75196 Genomic DNA. Translation: CAA99515.1.
    BK006948 Genomic DNA. Translation: DAA11052.1.
    PIRiS67190.
    RefSeqiNP_014931.3. NM_001183707.3.

    Genome annotation databases

    EnsemblFungiiYOR288C; YOR288C; YOR288C.
    GeneIDi854462.
    KEGGisce:YOR288C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D34633 Genomic DNA. Translation: BAA07015.1 .
    X89633 Genomic DNA. Translation: CAA61791.1 .
    Z75196 Genomic DNA. Translation: CAA99515.1 .
    BK006948 Genomic DNA. Translation: DAA11052.1 .
    PIRi S67190.
    RefSeqi NP_014931.3. NM_001183707.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ED3 X-ray 2.00 A/B 23-310 [» ]
    ProteinModelPortali Q12404.
    SMRi Q12404. Positions 22-301.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34675. 25 interactions.
    DIPi DIP-4085N.
    IntActi Q12404. 3 interactions.
    MINTi MINT-479800.
    STRINGi 4932.YOR288C.

    Proteomic databases

    MaxQBi Q12404.
    PaxDbi Q12404.
    PeptideAtlasi Q12404.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR288C ; YOR288C ; YOR288C .
    GeneIDi 854462.
    KEGGi sce:YOR288C.

    Organism-specific databases

    CYGDi YOR288c.
    SGDi S000005814. MPD1.

    Phylogenomic databases

    eggNOGi COG0526.
    GeneTreei ENSGT00730000110455.
    HOGENOMi HOG000248396.
    KOi K09584.
    OMAi RDENIME.
    OrthoDBi EOG71CFZN.

    Enzyme and pathway databases

    BioCyci YEAST:YOR288C-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12404.
    NextBioi 976742.

    Gene expression databases

    Genevestigatori Q12404.

    Family and domain databases

    Gene3Di 3.40.30.10. 1 hit.
    InterProi IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 1 hit.
    PS51352. THIOREDOXIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion."
      Tachikawa H., Takeuchi Y., Funahashi W., Miura T., Gao X.D., Fujimoto D., Mizunaga T., Onodera K.
      FEBS Lett. 369:212-216(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
      Cheret G., Bernardi A., Sor F.J.
      Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities."
      Kimura T., Hosoda Y., Sato Y., Kitamura Y., Ikeda T., Horibe T., Kikuchi M.
      J. Biol. Chem. 280:31438-31441(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CNE1 AND EPS1.

    Entry informationi

    Entry nameiMPD1_YEAST
    AccessioniPrimary (citable) accession number: Q12404
    Secondary accession number(s): D6W2Y6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 830 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3