Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein disulfide-isomerase MPD1

Gene

MPD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in the folding of proteins containing disulfide bonds.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

  • protein disulfide isomerase activity Source: SGD
  • protein disulfide oxidoreductase activity Source: SGD
  • protein-disulfide reductase (glutathione) activity Source: SGD

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • protein folding Source: SGD
  • response to endoplasmic reticulum stress Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciYEAST:YOR288C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase MPD1 (EC:5.3.4.1)
Gene namesi
Name:MPD1
Ordered Locus Names:YOR288C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR288C.
SGDiS000005814. MPD1.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000003422022 – 318Protein disulfide-isomerase MPD1Add BLAST297

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi59 ↔ 62Redox-activePROSITE-ProRule annotation
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ12404.
PRIDEiQ12404.

Interactioni

Subunit structurei

Interacts with CNE1 and EPS1.1 Publication

Protein-protein interaction databases

BioGridi34675. 28 interactors.
DIPiDIP-4085N.
IntActiQ12404. 3 interactors.
MINTiMINT-479800.

Structurei

Secondary structure

1318
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 43Combined sources8
Beta strandi45 – 48Combined sources4
Beta strandi50 – 55Combined sources6
Helixi60 – 63Combined sources4
Helixi66 – 75Combined sources10
Turni76 – 79Combined sources4
Beta strandi80 – 86Combined sources7
Turni90 – 92Combined sources3
Helixi93 – 98Combined sources6
Beta strandi103 – 111Combined sources9
Beta strandi132 – 135Combined sources4
Helixi142 – 150Combined sources9
Beta strandi157 – 159Combined sources3
Helixi163 – 165Combined sources3
Helixi166 – 170Combined sources5
Beta strandi174 – 186Combined sources13
Helixi189 – 197Combined sources9
Beta strandi202 – 208Combined sources7
Helixi209 – 211Combined sources3
Helixi226 – 239Combined sources14
Beta strandi246 – 251Combined sources6
Turni252 – 255Combined sources4
Beta strandi256 – 259Combined sources4
Helixi267 – 278Combined sources12
Beta strandi283 – 285Combined sources3
Helixi289 – 299Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ED3X-ray2.00A/B23-310[»]
ProteinModelPortaliQ12404.
SMRiQ12404.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12404.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 158ThioredoxinPROSITE-ProRule annotationAdd BLAST137

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi315 – 318Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

GeneTreeiENSGT00860000133723.
HOGENOMiHOG000248396.
InParanoidiQ12404.
KOiK09584.
OMAiKSIALDW.
OrthoDBiEOG092C3SRA.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12404-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFLNIIKLL LGLFIMNEVK AQNFYDSDPH ISELTPKSFD KAIHNTNYTS
60 70 80 90 100
LVEFYAPWCG HCKKLSSTFR KAAKRLDGVV QVAAVNCDLN KNKALCAKYD
110 120 130 140 150
VNGFPTLMVF RPPKIDLSKP IDNAKKSFSA HANEVYSGAR TLAPIVDFSL
160 170 180 190 200
SRIRSYVKKF VRIDTLGSLL RKSPKLSVVL FSKQDKISPV YKSIALDWLG
210 220 230 240 250
KFDFYSISNK KLKQLTDMNP TYEKTPEIFK YLQKVIPEQR QSDKSKLVVF
260 270 280 290 300
DADKDKFWEY EGNSINKNDI SKFLRDTFSI TPNEGPFSRR SEYIAYLKTG
310
KKPIKKNHSS SGNKHDEL
Length:318
Mass (Da):36,408
Last modified:November 1, 1996 - v1
Checksum:i3F96477E8182DC5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D34633 Genomic DNA. Translation: BAA07015.1.
X89633 Genomic DNA. Translation: CAA61791.1.
Z75196 Genomic DNA. Translation: CAA99515.1.
BK006948 Genomic DNA. Translation: DAA11052.1.
PIRiS67190.
RefSeqiNP_014931.3. NM_001183707.3.

Genome annotation databases

EnsemblFungiiYOR288C; YOR288C; YOR288C.
GeneIDi854462.
KEGGisce:YOR288C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D34633 Genomic DNA. Translation: BAA07015.1.
X89633 Genomic DNA. Translation: CAA61791.1.
Z75196 Genomic DNA. Translation: CAA99515.1.
BK006948 Genomic DNA. Translation: DAA11052.1.
PIRiS67190.
RefSeqiNP_014931.3. NM_001183707.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ED3X-ray2.00A/B23-310[»]
ProteinModelPortaliQ12404.
SMRiQ12404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34675. 28 interactors.
DIPiDIP-4085N.
IntActiQ12404. 3 interactors.
MINTiMINT-479800.

Proteomic databases

MaxQBiQ12404.
PRIDEiQ12404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR288C; YOR288C; YOR288C.
GeneIDi854462.
KEGGisce:YOR288C.

Organism-specific databases

EuPathDBiFungiDB:YOR288C.
SGDiS000005814. MPD1.

Phylogenomic databases

GeneTreeiENSGT00860000133723.
HOGENOMiHOG000248396.
InParanoidiQ12404.
KOiK09584.
OMAiKSIALDW.
OrthoDBiEOG092C3SRA.

Enzyme and pathway databases

BioCyciYEAST:YOR288C-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12404.
PROiQ12404.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPD1_YEAST
AccessioniPrimary (citable) accession number: Q12404
Secondary accession number(s): D6W2Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.