Reviewed,
UniProtKB/Swiss-Prot Q12404 (MPD1_YEAST)
Last modified
June 16, 2009.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Protein disulfide-isomerase MPD1 EC=5.3.4.1 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 318 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Participates in the folding of proteins containing disulfide bonds. |
| Catalytic activity | Catalyzes the rearrangement of -S-S- bonds in proteins. |
| Subcellular location | Endoplasmic reticulum lumen Potential. |
| Miscellaneous | Present with 830 molecules/cell in log phase SD medium. Ref.4 |
| Sequence similarities | Belongs to the protein disulfide isomerase family. Contains 1 thioredoxin domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum |
| Domain | Redox-active center Signal |
| Molecular function | Isomerase |
| PTM | Disulfide bond Glycoprotein |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | cell redox homeostasis Inferred from electronic annotation. Source: InterPro protein foldingInferred from genetic interaction. Source: SGD |
| Cellular component | endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumenInferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuoleInferred from direct assay. Source: SGD |
| Molecular function | protein disulfide isomerase activity Inferred from direct assay. Source: SGD protein disulfide oxidoreductase activityInferred from direct assay. Source: SGD |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 21 | 21 | Potential | ||||||||
| Chain | 22 – 318 | 297 | Protein disulfide-isomerase MPD1 | PRO_0000034220 | |||||||
Regions | |||||||||||
| Domain | 22 – 158 | 137 | Thioredoxin | ||||||||
| Motif | 315 – 318 | 4 | Prevents secretion from ER Potential | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 47 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 307 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 59 ↔ 62 | Redox-active By similarity | |||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion." Tachikawa H., Takeuchi Y., Funahashi W., Miura T., Gao X.D., Fujimoto D., Mizunaga T., Onodera K. FEBS Lett. 369:212-216(1995) [PubMed: 7649260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae." Cheret G., Bernardi A., Sor F.J. Yeast 12:1059-1064(1996) [PubMed: 8896271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K.Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [4] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D34633 Genomic DNA. Translation: BAA07015.1. X89633 Genomic DNA. Translation: CAA61791.1. Z75196 Genomic DNA. Translation: CAA99515.1. | |||||||||||||
| PIR | S67190. | ||||||||||||
| RefSeq | NP_014931.1. | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP:4085N. | ||||||||||||
| IntAct | Q12404. 1 interaction. | ||||||||||||
Proteomic databases | |||||||||||||
| PeptideAtlas | Q12404. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | YOR288C. Saccharomyces cerevisiae. [Contig view] | ||||||||||||
| GeneID | 854462. | ||||||||||||
| GenomeReviews | Gene locus YOR288C in contig Y13140_GR. | ||||||||||||
| KEGG | sce:YOR288C. | ||||||||||||
| NMPDR | fig|4932.3.peg.6043. | ||||||||||||
Organism-specific databases | |||||||||||||
| CYGD | YOR288c. | ||||||||||||
| SGD | S000005814. MPD1. | ||||||||||||
| Yeast-GFP | Search... | ||||||||||||
Phylogenomic databases | |||||||||||||
| HOGENOM | Q12404. | ||||||||||||
| OMA | Q12404. TTLVEFY. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 5.3.4.1. 250. | ||||||||||||
Gene expression databases | |||||||||||||
| GermOnline | YOR288C. Saccharomyces cerevisiae. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR000886. ER_targeting_sequence. IPR017936. Thioredoxin-like. IPR006662. Thioredoxin-like_subdom. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit. | ||||||||||||
| Pfam | PF00085. Thioredoxin. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00421. THIOREDOXIN. | ||||||||||||
| PROSITE | PS00014. ER_TARGET. 1 hit. PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| NextBio | 976742. | ||||||||||||
Entry information
| Entry name | MPD1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q12404 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


