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Q12404

- MPD1_YEAST

UniProt

Q12404 - MPD1_YEAST

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Protein

Protein disulfide-isomerase MPD1

Gene
MPD1, YOR288C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in the folding of proteins containing disulfide bonds.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

GO - Molecular functioni

  1. protein binding Source: SGD
  2. protein disulfide isomerase activity Source: SGD
  3. protein disulfide oxidoreductase activity Source: SGD
  4. protein-disulfide reductase (glutathione) activity Source: SGD

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. oxidation-reduction process Source: GOC
  3. protein folding Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciYEAST:YOR288C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase MPD1 (EC:5.3.4.1)
Gene namesi
Name:MPD1
Ordered Locus Names:YOR288C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR288c.
SGDiS000005814. MPD1.

Subcellular locationi

Endoplasmic reticulum lumen Reviewed prediction

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed predictionAdd
BLAST
Chaini22 – 318297Protein disulfide-isomerase MPD1PRO_0000034220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi59 ↔ 62Redox-active By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ12404.
PaxDbiQ12404.
PeptideAtlasiQ12404.

Expressioni

Gene expression databases

GenevestigatoriQ12404.

Interactioni

Subunit structurei

Interacts with CNE1 and EPS1.1 Publication

Protein-protein interaction databases

BioGridi34675. 25 interactions.
DIPiDIP-4085N.
IntActiQ12404. 3 interactions.
MINTiMINT-479800.
STRINGi4932.YOR288C.

Structurei

Secondary structure

1
318
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 438
Beta strandi45 – 484
Beta strandi50 – 556
Helixi60 – 634
Helixi66 – 7510
Turni76 – 794
Beta strandi80 – 867
Turni90 – 923
Helixi93 – 986
Beta strandi103 – 1119
Beta strandi132 – 1354
Helixi142 – 1509
Beta strandi157 – 1593
Helixi163 – 1653
Helixi166 – 1705
Beta strandi174 – 18613
Helixi189 – 1979
Beta strandi202 – 2087
Helixi209 – 2113
Helixi226 – 23914
Beta strandi246 – 2516
Turni252 – 2554
Beta strandi256 – 2594
Helixi267 – 27812
Beta strandi283 – 2853
Helixi289 – 29911

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ED3X-ray2.00A/B23-310[»]
ProteinModelPortaliQ12404.
SMRiQ12404. Positions 22-301.

Miscellaneous databases

EvolutionaryTraceiQ12404.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 158137ThioredoxinAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi315 – 3184Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Contains 1 thioredoxin domain.

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiCOG0526.
GeneTreeiENSGT00730000110455.
HOGENOMiHOG000248396.
KOiK09584.
OMAiRDENIME.
OrthoDBiEOG71CFZN.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12404-1 [UniParc]FASTAAdd to Basket

« Hide

MLFLNIIKLL LGLFIMNEVK AQNFYDSDPH ISELTPKSFD KAIHNTNYTS    50
LVEFYAPWCG HCKKLSSTFR KAAKRLDGVV QVAAVNCDLN KNKALCAKYD 100
VNGFPTLMVF RPPKIDLSKP IDNAKKSFSA HANEVYSGAR TLAPIVDFSL 150
SRIRSYVKKF VRIDTLGSLL RKSPKLSVVL FSKQDKISPV YKSIALDWLG 200
KFDFYSISNK KLKQLTDMNP TYEKTPEIFK YLQKVIPEQR QSDKSKLVVF 250
DADKDKFWEY EGNSINKNDI SKFLRDTFSI TPNEGPFSRR SEYIAYLKTG 300
KKPIKKNHSS SGNKHDEL 318
Length:318
Mass (Da):36,408
Last modified:November 1, 1996 - v1
Checksum:i3F96477E8182DC5C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D34633 Genomic DNA. Translation: BAA07015.1.
X89633 Genomic DNA. Translation: CAA61791.1.
Z75196 Genomic DNA. Translation: CAA99515.1.
BK006948 Genomic DNA. Translation: DAA11052.1.
PIRiS67190.
RefSeqiNP_014931.3. NM_001183707.3.

Genome annotation databases

EnsemblFungiiYOR288C; YOR288C; YOR288C.
GeneIDi854462.
KEGGisce:YOR288C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D34633 Genomic DNA. Translation: BAA07015.1 .
X89633 Genomic DNA. Translation: CAA61791.1 .
Z75196 Genomic DNA. Translation: CAA99515.1 .
BK006948 Genomic DNA. Translation: DAA11052.1 .
PIRi S67190.
RefSeqi NP_014931.3. NM_001183707.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3ED3 X-ray 2.00 A/B 23-310 [» ]
ProteinModelPortali Q12404.
SMRi Q12404. Positions 22-301.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34675. 25 interactions.
DIPi DIP-4085N.
IntActi Q12404. 3 interactions.
MINTi MINT-479800.
STRINGi 4932.YOR288C.

Proteomic databases

MaxQBi Q12404.
PaxDbi Q12404.
PeptideAtlasi Q12404.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR288C ; YOR288C ; YOR288C .
GeneIDi 854462.
KEGGi sce:YOR288C.

Organism-specific databases

CYGDi YOR288c.
SGDi S000005814. MPD1.

Phylogenomic databases

eggNOGi COG0526.
GeneTreei ENSGT00730000110455.
HOGENOMi HOG000248396.
KOi K09584.
OMAi RDENIME.
OrthoDBi EOG71CFZN.

Enzyme and pathway databases

BioCyci YEAST:YOR288C-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12404.
NextBioi 976742.

Gene expression databases

Genevestigatori Q12404.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 1 hit.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion."
    Tachikawa H., Takeuchi Y., Funahashi W., Miura T., Gao X.D., Fujimoto D., Mizunaga T., Onodera K.
    FEBS Lett. 369:212-216(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
    Cheret G., Bernardi A., Sor F.J.
    Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Interactions among yeast protein-disulfide isomerase proteins and endoplasmic reticulum chaperone proteins influence their activities."
    Kimura T., Hosoda Y., Sato Y., Kitamura Y., Ikeda T., Horibe T., Kikuchi M.
    J. Biol. Chem. 280:31438-31441(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CNE1 AND EPS1.

Entry informationi

Entry nameiMPD1_YEAST
AccessioniPrimary (citable) accession number: Q12404
Secondary accession number(s): D6W2Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 3, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi