ID   TRM10_YEAST             Reviewed;         293 AA.
AC   Q12400;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=tRNA (guanine-N(1)-)-methyltransferase TRM10;
DE            Short=tRNA methyltransferase 10;
DE            EC=2.1.1.31;
GN   Name=TRM10; OrderedLocusNames=YOL093W; ORFNames=O0926;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=96021609; PubMed=8533473; DOI=10.1002/yea.320111009;
RA   Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.;
RT   "A 29.425 kb segment on the left arm of yeast chromosome XV contains
RT   more than twice as many unknown as known open reading frames.";
RL   Yeast 11:975-986(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   MEDLINE=97313270; PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W.,
RA   Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R.,
RA   Boyer J., Camasses A., Casamayor A., Casas C., Cheret G.,
RA   Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H.,
RA   Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F.,
RA   Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A.,
RA   Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J.,
RA   Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P.,
RA   Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M.,
RA   Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R.,
RA   Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S.,
RA   Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A.,
RA   Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M.,
RA   Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C.,
RA   Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S.,
RA   Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [5]
RP   FUNCTION.
RX   MEDLINE=22588906; PubMed=12702816; DOI=10.1261/rna.5070303;
RA   Jackman J.E., Montange R.K., Malik H.S., Phizicky E.M.;
RT   "Identification of the yeast gene encoding the tRNA m1G
RT   methyltransferase responsible for modification at position 9.";
RL   RNA 9:574-585(2003).
RN   [6]
RP   FUNCTION.
RX   PubMed=15640439; DOI=10.1093/nar/gni002;
RA   Hiley S.L., Jackman J.E., Babak T., Trochesset M., Morris Q.D.,
RA   Phizicky E.M., Hughes T.R.;
RT   "Detection and discovery of RNA modifications using microarrays.";
RL   Nucleic Acids Res. 33:E2-E2(2005).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND THR-16, AND MASS
RP   SPECTROMETRY.
RX   PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth
RT   phosphoproteome analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
CC   -!- FUNCTION: Catalyzes the formation of N(1)-methylguanine at
CC       position 9 (m1G9) in cytoplasmic tRNAs.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(1)-methylguanine.
CC   -!- INTERACTION:
CC       Self; NbExp=1; IntAct=EBI-29692, EBI-29692;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC   -!- MISCELLANEOUS: Present with 4090 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the RNA methyltransferase trmD family.
CC       TRM10 subfamily.
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DR   EMBL; X83121; CAA58186.1; -; Genomic_DNA.
DR   EMBL; Z74835; CAA99105.1; -; Genomic_DNA.
DR   PIR; S57376; S57376.
DR   RefSeq; NP_014548.1; -.
DR   DIP; DIP:4737N; -.
DR   IntAct; Q12400; 3.
DR   Ensembl; YOL093W; Saccharomyces cerevisiae.
DR   GeneID; 854060; -.
DR   GenomeReviews; Y13140_GR; YOL093W.
DR   KEGG; sce:YOL093W; -.
DR   NMPDR; fig|4932.3.peg.5637; -.
DR   CYGD; YOL093w; -.
DR   SGD; S000005453; TRM10.
DR   HOGENOM; Q12400; -.
DR   OMA; Q12400; RKRIRRD.
DR   BRENDA; 2.1.1.31; 250.
DR   NextBio; 975659; -.
DR   ArrayExpress; Q12400; -.
DR   GermOnline; YOL093W; Saccharomyces cerevisiae.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0009019; F:tRNA (guanine-N1-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0030488; P:tRNA methylation; IDA:SGD.
DR   InterPro; IPR016009; tRNA_m1G_MeTrfase.
DR   InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR   InterPro; IPR016653; tRNA_m1G_mtfrase_met.
DR   PANTHER; PTHR13563; DUF425; 1.
DR   Pfam; PF01746; tRNA_m1G_MT; 1.
DR   PIRSF; PIRSF016323; tRNA_m1G_mtfrase_met; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Cytoplasm; Methyltransferase; Nucleus;
KW   Phosphoprotein; S-adenosyl-L-methionine; Transferase; tRNA processing.
FT   CHAIN         1    293       tRNA (guanine-N(1)-)-methyltransferase
FT                                TRM10.
FT                                /FTId=PRO_0000060520.
FT   MOD_RES       2      2       Phosphoserine.
FT   MOD_RES      16     16       Phosphothreonine.
SQ   SEQUENCE   293 AA;  34520 MW;  C3DC7C4BB2FFBE63 CRC64;
     MSNDEINQNE EKVKRTPPLP PVPEGMSKKQ WKKMCKRQRW EENKAKYNAE RRVKKKRLRH
     ERSAKIQEYI DRGEEVPQEL IREPRINVNQ TDSGIEIILD CSFDELMNDK EIVSLSNQVT
     RAYSANRRAN HFAEIKVAPF DKRLKQRFET TLKNTNYENW NHFKFLPDDK IMFGDEHISK
     DKIVYLTADT EEKLEKLEPG MRYIVGGIVD KNRYKELCLK KAQKMGIPTR RLPIDEYINL
     EGRRVLTTTH VVQLMLKYFD DHNWKNAFES VLPPRKLDAE AKSASSSPAP KDT
//