Reviewed, UniProtKB/Swiss-Prot Q12400 (TRM10_YEAST)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase TRM10
      Short name=tRNA methyltransferase 10
    EC=2.1.1.31

Gene names

Name:	TRM10
Ordered Locus Names:	YOL093W
ORF Names:	O0926

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs. Ref.5 Ref.6
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine.
Subcellular location	Cytoplasm. Nucleus. Ref.3
Miscellaneous	Present with 4090 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the RNA methyltransferase trmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA methylation Ref.5 Inferred from direct assay. Source: SGD
Cellular component	cytoplasm Ref.3 Inferred from direct assay. Source: SGD nucleus Ref.3 Inferred from direct assay. Source: SGD
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-29692,EBI-29692

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	tRNA (guanine-N(1)-)-methyltransferase TRM10		PRO_0000060520
Amino acid modifications
	Modified residue	2	1	Phosphoserine Ref.7
	Modified residue	16	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q12400-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C3DC7C4BB2FFBE63	FASTA	293	34,520
	10 20 30 40 50 60 MSNDEINQNE EKVKRTPPLP PVPEGMSKKQ WKKMCKRQRW EENKAKYNAE RRVKKKRLRH 70 80 90 100 110 120 ERSAKIQEYI DRGEEVPQEL IREPRINVNQ TDSGIEIILD CSFDELMNDK EIVSLSNQVT 130 140 150 160 170 180 RAYSANRRAN HFAEIKVAPF DKRLKQRFET TLKNTNYENW NHFKFLPDDK IMFGDEHISK 190 200 210 220 230 240 DKIVYLTADT EEKLEKLEPG MRYIVGGIVD KNRYKELCLK KAQKMGIPTR RLPIDEYINL 250 260 270 280 290 EGRRVLTTTH VVQLMLKYFD DHNWKNAFES VLPPRKLDAE AKSASSSPAP KDT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames." Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M. Yeast 11:975-986(1995) [PubMed: 8533473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed: 9169874] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Identification of the yeast gene encoding the tRNA m1G methyltransferase responsible for modification at position 9." Jackman J.E., Montange R.K., Malik H.S., Phizicky E.M. RNA 9:574-585(2003) [PubMed: 12702816] [Abstract] Cited for: FUNCTION.
[6]	"Detection and discovery of RNA modifications using microarrays." Hiley S.L., Jackman J.E., Babak T., Trochesset M., Morris Q.D., Phizicky E.M., Hughes T.R. Nucleic Acids Res. 33:E2-E2(2005) [PubMed: 15640439] [Abstract] Cited for: FUNCTION.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND THR-16, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83121 Genomic DNA. Translation: CAA58186.1. Z74835 Genomic DNA. Translation: CAA99105.1.
PIR	S57376.
RefSeq	NP_014548.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4737N.
IntAct	Q12400. 3 interactions.
Genome annotation databases
Ensembl	YOL093W. Saccharomyces cerevisiae. [Contig view]
GeneID	854060.
GenomeReviews	Gene locus YOL093W in contig Y13140_GR.
KEGG	sce:YOL093W.
NMPDR	fig|4932.3.peg.5637.
Organism-specific databases
CYGD	YOL093w.
SGD	S000005453. TRM10.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12400.
OMA	Q12400. RKRIRRD.
Enzyme and pathway databases
BRENDA	2.1.1.31. 250.
Gene expression databases
ArrayExpress	Q12400.
GermOnline	YOL093W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. IPR016653. tRNA_m1G_mtfrase_met. [Graphical view]
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF016323. tRNA_m1G_mtfrase_met. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	975659.

Entry information

Entry name

TRM10_YEAST

Accession

Primary (citable) accession number: Q12400

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)