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Protein

tRNA (guanine(9)-N1)-methyltransferase

Gene

TRM10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent guanine N1-methyltransferase that catalyzes the formation of N1-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs.3 Publications

Miscellaneous

Present with 4090 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + guanine9 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA.2 Publications

Kineticsi

kcat is 0.54 min(-1) for tRNA(Gly GCC) and 0.31 min(-1) for tRNA(Val UAC) (m1G9)-methylation, respectively.1 Publication
  1. KM=2400 nM for tRNA(Gly GCC)1 Publication
  2. KM=1860 nM for tRNA(Val UAC)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei206S-adenosyl-L-methionine; via amide nitrogen1
    Active sitei210Proton acceptor1 Publication1
    Binding sitei218S-adenosyl-L-methionine1
    Binding sitei232S-adenosyl-L-methionine; via amide nitrogen1

    GO - Molecular functioni

    GO - Biological processi

    • tRNA methylation Source: SGD
    • tRNA modification Source: Reactome

    Keywordsi

    Molecular functionMethyltransferase, Transferase
    Biological processtRNA processing
    LigandS-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciMetaCyc:G3O-33493-MONOMER
    YEAST:G3O-33493-MONOMER
    BRENDAi2.1.1.221 984

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine(9)-N1)-methyltransferase1 Publication (EC:2.1.1.2212 Publications)
    Alternative name(s):
    tRNA methyltransferase 101 Publication
    tRNA(m1G9)-methyltransferase1 Publication
    Short name:
    tRNA(m1G9)MTase1 Publication
    Gene namesi
    Name:TRM101 Publication
    Ordered Locus Names:YOL093WImported
    ORF Names:O0926
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOL093W
    SGDiS000005453 TRM10

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi118Q → A: Completely abolishes catalytic activity. 1 Publication1
    Mutagenesisi209V → A: Reduces catalytic activity. 1 Publication1
    Mutagenesisi210D → N: Completely abolishes catalytic activity. 1 Publication1
    Mutagenesisi211K → A: Reduces catalytic activity. 1 Publication1
    Mutagenesisi212N → A: Has weaker affinity for S-adenosyl-L-methionine and reduces catalytic activity by 90%. 1 Publication1
    Mutagenesisi247T → A: Reduces catalytic activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000605201 – 293tRNA (guanine(9)-N1)-methyltransferaseAdd BLAST293

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei16PhosphothreonineCombined sources1
    Modified residuei283PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12400
    PaxDbiQ12400
    PRIDEiQ12400

    PTM databases

    iPTMnetiQ12400

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi34309, 87 interactors
    DIPiDIP-4737N
    IntActiQ12400, 2 interactors
    MINTiQ12400
    STRINGi4932.YOL093W

    Structurei

    Secondary structure

    1293
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi91 – 100Combined sources10
    Helixi104 – 106Combined sources3
    Helixi109 – 128Combined sources20
    Beta strandi129 – 139Combined sources11
    Helixi142 – 150Combined sources9
    Helixi153 – 155Combined sources3
    Helixi157 – 159Combined sources3
    Beta strandi163 – 165Combined sources3
    Beta strandi167 – 169Combined sources3
    Helixi170 – 173Combined sources4
    Beta strandi176 – 178Combined sources3
    Helixi180 – 182Combined sources3
    Beta strandi183 – 186Combined sources4
    Beta strandi191 – 193Combined sources3
    Beta strandi202 – 206Combined sources5
    Helixi217 – 225Combined sources9
    Beta strandi229 – 231Combined sources3
    Helixi235 – 237Combined sources3
    Helixi248 – 258Combined sources11
    Turni259 – 262Combined sources4
    Helixi264 – 271Combined sources8

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4JWJX-ray1.76A/B84-276[»]
    ProteinModelPortaliQ12400
    SMRiQ12400
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini83 – 279SAM-dependent MTase TRM10-typePROSITE-ProRule annotationAdd BLAST197

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni186 – 187S-adenosyl-L-methionine binding2
    Regioni210 – 214S-adenosyl-L-methionine binding5
    Regioni244 – 246S-adenosyl-L-methionine binding3

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili32 – 61Sequence analysisAdd BLAST30

    Sequence similaritiesi

    Belongs to the class IV-like SAM-binding methyltransferase superfamily. TRM10 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    GeneTreeiENSGT00530000063169
    HOGENOMiHOG000195825
    InParanoidiQ12400
    KOiK15445
    OMAiKKNDGWE
    OrthoDBiEOG092C39DJ

    Family and domain databases

    Gene3Di3.40.1280.30, 1 hit
    InterProiView protein in InterPro
    IPR028564 MT_TRM10-typ
    IPR038459 MT_TRM10-typ_sf
    IPR016653 TRM10/TRM10A
    IPR007356 tRNA_m1G_MeTrfase_euk
    IPR016009 tRNA_MeTrfase_TRMD/TRM10
    PANTHERiPTHR13563 PTHR13563, 1 hit
    PTHR13563:SF13 PTHR13563:SF13, 1 hit
    PfamiView protein in Pfam
    PF01746 tRNA_m1G_MT, 1 hit
    PIRSFiPIRSF016323 tRNA_m1G_mtfrase_met, 1 hit
    PROSITEiView protein in PROSITE
    PS51675 SAM_MT_TRM10, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q12400-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSNDEINQNE EKVKRTPPLP PVPEGMSKKQ WKKMCKRQRW EENKAKYNAE
    60 70 80 90 100
    RRVKKKRLRH ERSAKIQEYI DRGEEVPQEL IREPRINVNQ TDSGIEIILD
    110 120 130 140 150
    CSFDELMNDK EIVSLSNQVT RAYSANRRAN HFAEIKVAPF DKRLKQRFET
    160 170 180 190 200
    TLKNTNYENW NHFKFLPDDK IMFGDEHISK DKIVYLTADT EEKLEKLEPG
    210 220 230 240 250
    MRYIVGGIVD KNRYKELCLK KAQKMGIPTR RLPIDEYINL EGRRVLTTTH
    260 270 280 290
    VVQLMLKYFD DHNWKNAFES VLPPRKLDAE AKSASSSPAP KDT
    Length:293
    Mass (Da):34,520
    Last modified:November 1, 1996 - v1
    Checksum:iC3DC7C4BB2FFBE63
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X83121 Genomic DNA Translation: CAA58186.1
    Z74835 Genomic DNA Translation: CAA99105.1
    BK006948 Genomic DNA Translation: DAA10691.1
    PIRiS57376
    RefSeqiNP_014548.1, NM_001183347.1

    Genome annotation databases

    EnsemblFungiiYOL093W; YOL093W; YOL093W
    GeneIDi854060
    KEGGisce:YOL093W

    Similar proteinsi

    Entry informationi

    Entry nameiTRM10_YEAST
    AccessioniPrimary (citable) accession number: Q12400
    Secondary accession number(s): D6W1X5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 1, 1996
    Last modified: March 28, 2018
    This is version 132 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health