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Protein

Protein EMP46

Gene

EMP46

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi177Potassium1

GO - Molecular functioni

  • glycoprotein binding Source: SGD
  • mannose binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • endoplasmic reticulum organization Source: GO_Central
  • ER to Golgi vesicle-mediated transport Source: SGD
  • Golgi organization Source: GO_Central
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lectin, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciYEAST:G3O-32231-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein EMP46
Alternative name(s):
46 kDa endomembrane protein
Gene namesi
Name:EMP46
Ordered Locus Names:YLR080W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR080W.
SGDiS000004070. EMP46.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini47 – 408LumenalSequence analysisAdd BLAST362
Transmembranei409 – 429HelicalSequence analysisAdd BLAST21
Topological domaini430 – 444CytoplasmicSequence analysisAdd BLAST15

GO - Cellular componenti

  • endoplasmic reticulum-Golgi intermediate compartment Source: GO_Central
  • endoplasmic reticulum membrane Source: GO_Central
  • ER to Golgi transport vesicle Source: SGD
  • Golgi membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi177Y → F: Impairs potassium-binding. 1 Publication1
Mutagenesisi429Y → A: Impairs interaction with COP1, SEC21 and SEC23 and loss of reticulum endoplasmic exit. 1 Publication1
Mutagenesisi432F → A: Impairs interaction with COP1, SEC21 and SEC23 and loss of reticulum endoplasmic exit. 1 Publication1
Mutagenesisi440 – 444Missing : Loss of endoplasmic reticulum exit. 1 Publication5
Mutagenesisi440 – 442KVK → SVS or RVR: Impairs interaction with COP1 and SEC21 and mislocalizes to the vacuole. 1 Publication3
Mutagenesisi443 – 444LL → AA: Loss of endoplasmic reticulum exit. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 461 PublicationAdd BLAST46
ChainiPRO_000023964547 – 444Protein EMP46Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi196 ↔ 230

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ12396.
PRIDEiQ12396.

Interactioni

Subunit structurei

Interacts with EMP47 in the endoplasmic reticulum membrane in order to be transported to the Golgi apparatus. Interacts with the coatomer proteins COP1, SEC21 and SEC23.2 Publications

Protein-protein interaction databases

BioGridi31353. 23 interactors.
DIPiDIP-4209N.
IntActiQ12396. 1 interactor.
MINTiMINT-497123.

Structurei

Secondary structure

1444
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi58 – 60Combined sources3
Turni65 – 67Combined sources3
Helixi71 – 74Combined sources4
Turni75 – 77Combined sources3
Beta strandi78 – 82Combined sources5
Beta strandi85 – 87Combined sources3
Beta strandi90 – 93Combined sources4
Beta strandi100 – 107Combined sources8
Beta strandi115 – 125Combined sources11
Beta strandi133 – 139Combined sources7
Turni150 – 152Combined sources3
Beta strandi158 – 167Combined sources10
Turni168 – 170Combined sources3
Beta strandi171 – 183Combined sources13
Turni187 – 189Combined sources3
Beta strandi193 – 197Combined sources5
Beta strandi207 – 214Combined sources8
Helixi215 – 217Combined sources3
Beta strandi219 – 225Combined sources7
Beta strandi228 – 234Combined sources7
Helixi238 – 240Combined sources3
Beta strandi244 – 251Combined sources8
Beta strandi258 – 269Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A6VX-ray1.52A/B52-275[»]
2A6WX-ray1.75A/B52-275[»]
2A6XX-ray1.55A/B52-275[»]
ProteinModelPortaliQ12396.
SMRiQ12396.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12396.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 269L-type lectin-likePROSITE-ProRule annotationAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni429 – 432Mediates the interactions with COPI and COPII coat complexes4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi440 – 444Di-lysine motif5

Domaini

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.By similarity

Sequence similaritiesi

Belongs to the EMP46/EMP47 family.Curated
Contains 1 L-type lectin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000053646.
HOGENOMiHOG000112365.
InParanoidiQ12396.
KOiK10080.
OMAiIEWTFRS.
OrthoDBiEOG092C4CI2.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016710. Emp46/Emp47.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
PIRSFiPIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12396-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRKTASSL QLLGKITGTK AGTKQKKMNF INGLIWLYMC VWMVHGKVTQ
60 70 80 90 100
KDELKWNKGY SLPNLLEVTD QQKELSQWTL GDKVKLEEGR FVLTPGKNTK
110 120 130 140 150
GSLWLKPEYS IKDAMTIEWT FRSFGFRGST KGGLAFWLKQ GNEGDSTELF
160 170 180 190 200
GGSSKKFNGL MILLRLDDKL GESVTAYLND GTKDLDIESS PYFASCLFQY
210 220 230 240 250
QDSMVPSTLR LTYNPLDNHL LKLQMDNRVC FQTRKVKFMG SSPFRIGTSA
260 270 280 290 300
INDASKESFE ILKMKLYDGV IEDSLIPNVN PMGQPRVVTK VINSQTGEES
310 320 330 340 350
FREKMPFSDK EESITSNELF EKMNKLEGKI MANDIDPLLR KMNKIVENER
360 370 380 390 400
ELIQRLRPLL DLKKTAISDD SFQDFLSMNA NLDRLIKEQE KIRQDAKLYG
410 420 430 440
KQTKGHDEIF SKISVWLALL IFIMITLAYY MFRINQDIKK VKLL
Length:444
Mass (Da):50,947
Last modified:November 1, 1996 - v1
Checksum:i9B83B61667814DBE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73252 Genomic DNA. Translation: CAA97639.1.
U53880 Genomic DNA. Translation: AAB67584.1.
BK006945 Genomic DNA. Translation: DAA09396.1.
PIRiS64912.
RefSeqiNP_013181.1. NM_001181967.1.

Genome annotation databases

EnsemblFungiiYLR080W; YLR080W; YLR080W.
GeneIDi850769.
KEGGisce:YLR080W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73252 Genomic DNA. Translation: CAA97639.1.
U53880 Genomic DNA. Translation: AAB67584.1.
BK006945 Genomic DNA. Translation: DAA09396.1.
PIRiS64912.
RefSeqiNP_013181.1. NM_001181967.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2A6VX-ray1.52A/B52-275[»]
2A6WX-ray1.75A/B52-275[»]
2A6XX-ray1.55A/B52-275[»]
ProteinModelPortaliQ12396.
SMRiQ12396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31353. 23 interactors.
DIPiDIP-4209N.
IntActiQ12396. 1 interactor.
MINTiMINT-497123.

Proteomic databases

MaxQBiQ12396.
PRIDEiQ12396.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR080W; YLR080W; YLR080W.
GeneIDi850769.
KEGGisce:YLR080W.

Organism-specific databases

EuPathDBiFungiDB:YLR080W.
SGDiS000004070. EMP46.

Phylogenomic databases

GeneTreeiENSGT00510000053646.
HOGENOMiHOG000112365.
InParanoidiQ12396.
KOiK10080.
OMAiIEWTFRS.
OrthoDBiEOG092C4CI2.

Enzyme and pathway databases

BioCyciYEAST:G3O-32231-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12396.
PROiQ12396.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016710. Emp46/Emp47.
IPR005052. Lectin_leg.
[Graphical view]
PfamiPF03388. Lectin_leg-like. 1 hit.
[Graphical view]
PIRSFiPIRSF018136. L-type_lectin_fungi. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS51328. L_LECTIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMP46_YEAST
AccessioniPrimary (citable) accession number: Q12396
Secondary accession number(s): D6VY80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.