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Q12390 (GST2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase 2

EC=2.5.1.18
Alternative name(s):
GST-II
Gene names
Name:GTT2
Ordered Locus Names:YLL060C
ORF Names:L0560
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length233 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

RX + glutathione = HX + R-S-glutathione. Ref.6

Subunit structure

Homodimer. Ref.6

Sequence similarities

Belongs to the GST superfamily.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Molecular functionTransferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutathione metabolic process

Inferred from direct assay Ref.5. Source: SGD

   Molecular_functionglutathione transferase activity

Inferred from direct assay Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 233233Glutathione S-transferase 2
PRO_0000185988

Regions

Domain17 – 10185GST N-terminal
Domain106 – 233128GST C-terminal
Region85 – 862Glutathione binding

Sites

Binding site291Glutathione; via amide nitrogen
Binding site581Glutathione
Binding site721Glutathione; via amide nitrogen and carbonyl oxygen
Binding site1331Glutathione

Experimental info

Mutagenesis271G → A: Reduced enzyme activity. Ref.6
Mutagenesis271G → C, F or S: Loss of enzyme activity. Ref.6
Mutagenesis1291S → A: Reduced enzyme activity. Ref.6
Mutagenesis1331H → A: Loss of enzyme activity. Ref.6

Secondary structure

...................................... 233
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12390 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: B5EE0E47D5A37175

FASTA23326,340
        10         20         30         40         50         60 
MNGRGFLIYN GGEKMKQKMI IYDTPAGPYP ARVRIALAEK NMLSSVQFVR INLWKGEHKK 

        70         80         90        100        110        120 
PEFLAKNYSG TVPVLELDDG TLIAECTAIT EYIDALDGTP TLTGKTPLEK GVIHMMNKRA 

       130        140        150        160        170        180 
ELELLDPVSV YFHHATPGLG PEVELYQNKE WGLRQRDKAL HGMHYFDTVL RERPYVAGDS 

       190        200        210        220        230 
FSMADITVIA GLIFAAIVKL QVPEECEALR AWYKRMQQRP SVKKLLEIRS KSS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a 37 kb DNA fragment from chromosome XII of Saccharomyces cerevisiae including the subtelomeric region of the left arm."
Wedler H., Wambutt R.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae."
Choi J.H., Lou W., Vancura A.
J. Biol. Chem. 273:29915-29922(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Structures of yeast glutathione-S-transferase Gtt2 reveal a new catalytic type of GST family."
Ma X.X., Jiang Y.L., He Y.X., Bao R., Chen Y., Zhou C.Z.
EMBO Rep. 10:1320-1326(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE, CATALYTIC ACTIVITY, MUTAGENESIS OF GLY-27; SER-129 AND HIS-133, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z47973 Genomic DNA. Translation: CAA87997.1.
Z73165 Genomic DNA. Translation: CAA97513.1.
AY557940 Genomic DNA. Translation: AAS56266.1.
BK006945 Genomic DNA. Translation: DAA09265.1.
PIRS50960.
RefSeqNP_013040.1. NM_001181880.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3ERFX-ray2.23A1-233[»]
3ERGX-ray2.20A/B1-233[»]
3IBHX-ray2.10A1-233[»]
ProteinModelPortalQ12390.
SMRQ12390. Positions 19-226.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31256. 19 interactions.
DIPDIP-2981N.
IntActQ12390. 1 interaction.
MINTMINT-2785201.
STRING4932.YLL060C.

Proteomic databases

PaxDbQ12390.
PeptideAtlasQ12390.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLL060C; YLL060C; YLL060C.
GeneID850666.
KEGGsce:YLL060C.

Organism-specific databases

CYGDYLL060c.
SGDS000003983. GTT2.

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125751.
OMAPYPARVR.
OrthoDBEOG7QNVXT.

Enzyme and pathway databases

BioCycYEAST:YLL060C-MONOMER.
SABIO-RKQ12390.

Gene expression databases

GenevestigatorQ12390.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ12390.
NextBio966644.
PROQ12390.

Entry information

Entry nameGST2_YEAST
AccessionPrimary (citable) accession number: Q12390
Secondary accession number(s): D6VXU9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references