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Q12390

- GST2_YEAST

UniProt

Q12390 - GST2_YEAST

Protein

Glutathione S-transferase 2

Gene

GTT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei29 – 291Glutathione; via amide nitrogen1 Publication
    Binding sitei58 – 581Glutathione1 Publication
    Binding sitei72 – 721Glutathione; via amide nitrogen and carbonyl oxygen1 Publication
    Binding sitei133 – 1331Glutathione1 Publication

    GO - Molecular functioni

    1. glutathione transferase activity Source: SGD

    GO - Biological processi

    1. glutathione metabolic process Source: SGD

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YLL060C-MONOMER.
    SABIO-RKQ12390.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase 2 (EC:2.5.1.18)
    Alternative name(s):
    GST-II
    Gene namesi
    Name:GTT2
    Ordered Locus Names:YLL060C
    ORF Names:L0560
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLL060c.
    SGDiS000003983. GTT2.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi27 – 271G → A: Reduced enzyme activity. 1 Publication
    Mutagenesisi27 – 271G → C, F or S: Loss of enzyme activity. 1 Publication
    Mutagenesisi129 – 1291S → A: Reduced enzyme activity. 1 Publication
    Mutagenesisi133 – 1331H → A: Loss of enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 233233Glutathione S-transferase 2PRO_0000185988Add
    BLAST

    Proteomic databases

    PaxDbiQ12390.
    PeptideAtlasiQ12390.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12390.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi31256. 19 interactions.
    DIPiDIP-2981N.
    IntActiQ12390. 1 interaction.
    MINTiMINT-2785201.
    STRINGi4932.YLL060C.

    Structurei

    Secondary structure

    1
    233
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi20 – 234
    Helixi28 – 3912
    Helixi43 – 453
    Beta strandi47 – 504
    Helixi53 – 553
    Helixi57 – 593
    Helixi61 – 666
    Beta strandi74 – 763
    Beta strandi82 – 854
    Helixi86 – 9611
    Beta strandi101 – 1033
    Helixi107 – 12317
    Helixi125 – 13511
    Turni141 – 1433
    Helixi149 – 17022
    Beta strandi178 – 1803
    Helixi183 – 19715
    Helixi207 – 21812
    Helixi220 – 2256

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3ERFX-ray2.23A1-233[»]
    3ERGX-ray2.20A/B1-233[»]
    3IBHX-ray2.10A1-233[»]
    ProteinModelPortaliQ12390.
    SMRiQ12390. Positions 19-226.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12390.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 10185GST N-terminalAdd
    BLAST
    Domaini106 – 233128GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni85 – 862Glutathione binding

    Sequence similaritiesi

    Belongs to the GST superfamily.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    HOGENOMiHOG000125751.
    OMAiPYPARVR.
    OrthoDBiEOG7QNVXT.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12390-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNGRGFLIYN GGEKMKQKMI IYDTPAGPYP ARVRIALAEK NMLSSVQFVR    50
    INLWKGEHKK PEFLAKNYSG TVPVLELDDG TLIAECTAIT EYIDALDGTP 100
    TLTGKTPLEK GVIHMMNKRA ELELLDPVSV YFHHATPGLG PEVELYQNKE 150
    WGLRQRDKAL HGMHYFDTVL RERPYVAGDS FSMADITVIA GLIFAAIVKL 200
    QVPEECEALR AWYKRMQQRP SVKKLLEIRS KSS 233
    Length:233
    Mass (Da):26,340
    Last modified:November 1, 1996 - v1
    Checksum:iB5EE0E47D5A37175
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47973 Genomic DNA. Translation: CAA87997.1.
    Z73165 Genomic DNA. Translation: CAA97513.1.
    AY557940 Genomic DNA. Translation: AAS56266.1.
    BK006945 Genomic DNA. Translation: DAA09265.1.
    PIRiS50960.
    RefSeqiNP_013040.1. NM_001181880.1.

    Genome annotation databases

    EnsemblFungiiYLL060C; YLL060C; YLL060C.
    GeneIDi850666.
    KEGGisce:YLL060C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z47973 Genomic DNA. Translation: CAA87997.1 .
    Z73165 Genomic DNA. Translation: CAA97513.1 .
    AY557940 Genomic DNA. Translation: AAS56266.1 .
    BK006945 Genomic DNA. Translation: DAA09265.1 .
    PIRi S50960.
    RefSeqi NP_013040.1. NM_001181880.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3ERF X-ray 2.23 A 1-233 [» ]
    3ERG X-ray 2.20 A/B 1-233 [» ]
    3IBH X-ray 2.10 A 1-233 [» ]
    ProteinModelPortali Q12390.
    SMRi Q12390. Positions 19-226.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31256. 19 interactions.
    DIPi DIP-2981N.
    IntActi Q12390. 1 interaction.
    MINTi MINT-2785201.
    STRINGi 4932.YLL060C.

    Proteomic databases

    PaxDbi Q12390.
    PeptideAtlasi Q12390.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLL060C ; YLL060C ; YLL060C .
    GeneIDi 850666.
    KEGGi sce:YLL060C.

    Organism-specific databases

    CYGDi YLL060c.
    SGDi S000003983. GTT2.

    Phylogenomic databases

    eggNOGi COG0625.
    HOGENOMi HOG000125751.
    OMAi PYPARVR.
    OrthoDBi EOG7QNVXT.

    Enzyme and pathway databases

    BioCyci YEAST:YLL060C-MONOMER.
    SABIO-RK Q12390.

    Miscellaneous databases

    EvolutionaryTracei Q12390.
    NextBioi 966644.
    PROi Q12390.

    Gene expression databases

    Genevestigatori Q12390.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a 37 kb DNA fragment from chromosome XII of Saccharomyces cerevisiae including the subtelomeric region of the left arm."
      Wedler H., Wambutt R.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "A novel membrane-bound glutathione S-transferase functions in the stationary phase of the yeast Saccharomyces cerevisiae."
      Choi J.H., Lou W., Vancura A.
      J. Biol. Chem. 273:29915-29922(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. "Structures of yeast glutathione-S-transferase Gtt2 reveal a new catalytic type of GST family."
      Ma X.X., Jiang Y.L., He Y.X., Bao R., Chen Y., Zhou C.Z.
      EMBO Rep. 10:1320-1326(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE, CATALYTIC ACTIVITY, MUTAGENESIS OF GLY-27; SER-129 AND HIS-133, SUBUNIT.

    Entry informationi

    Entry nameiGST2_YEAST
    AccessioniPrimary (citable) accession number: Q12390
    Secondary accession number(s): D6VXU9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3