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Protein

ATP-dependent RNA helicase DBP10

Gene

DBP10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi181 – 188ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • identical protein binding Source: IntAct
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • assembly of large subunit precursor of preribosome Source: SGD
  • maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29457-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DBP10 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 10
Gene namesi
Name:DBP10
Ordered Locus Names:YDL031W
ORF Names:D2770
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL031W
SGDiS000002189 DBP10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550401 – 995ATP-dependent RNA helicase DBP10Add BLAST995

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineCombined sources1
Modified residuei398PhosphoserineCombined sources1
Modified residuei400PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12389
PaxDbiQ12389
PRIDEiQ12389

PTM databases

iPTMnetiQ12389

Interactioni

Subunit structurei

Interacts with RRP1 and associates with pre-ribosomal particles.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi32025, 243 interactors
DIPiDIP-6387N
IntActiQ12389, 33 interactors
MINTiQ12389
STRINGi4932.YDL031W

Structurei

3D structure databases

ProteinModelPortaliQ12389
SMRiQ12389
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 340Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST173
Domaini418 – 568Helicase C-terminalPROSITE-ProRule annotationAdd BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi137 – 165Q motifAdd BLAST29
Motifi288 – 291DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075100
HOGENOMiHOG000246455
InParanoidiQ12389
KOiK14808
OMAiLHLCVEM
OrthoDBiEOG092C1VLP

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR012541 DBP10_C
IPR033517 DDX54/DBP10
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PANTHERiPTHR24031:SF292 PTHR24031:SF292, 1 hit
PfamiView protein in Pfam
PF08147 DBP10CT, 1 hit
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD024971 DBP10CT, 1 hit
SMARTiView protein in SMART
SM01123 DBP10CT, 1 hit
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 3 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

Q12389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVQKRKRD LEDQDDNGSE EDDIAFDIAN EIALNDSESD ANDSDSEVEA
60 70 80 90 100
DYGPNDVQDV IEYSSDEEEG VNNKKKAENK DIKKKKNSKK EIAAFPMLEM
110 120 130 140 150
SDDENNASGK TQTGDDEDDV NEYFSTNNLE KTKHKKGSFP SFGLSKIVLN
160 170 180 190 200
NIKRKGFRQP TPIQRKTIPL ILQSRDIVGM ARTGSGKTAA FILPMVEKLK
210 220 230 240 250
SHSGKIGARA VILSPSRELA MQTFNVFKDF ARGTELRSVL LTGGDSLEEQ
260 270 280 290 300
FGMMMTNPDV IIATPGRFLH LKVEMNLDLK SVEYVVFDEA DRLFEMGFQE
310 320 330 340 350
QLNELLASLP TTRQTLLFSA TLPNSLVDFV KAGLVNPVLV RLDAETKVSE
360 370 380 390 400
NLEMLFLSSK NADREANLLY ILQEIIKIPL ATSEQLQKLQ NSNNEADSDS
410 420 430 440 450
DDENDRQKKR RNFKKEKFRK QKMPAANELP SEKATILFVP TRHHVEYISQ
460 470 480 490 500
LLRDCGYLIS YIYGTLDQHA RKRQLYNFRA GLTSILVVTD VAARGVDIPM
510 520 530 540 550
LANVINYTLP GSSKIFVHRV GRTARAGNKG WAYSIVAENE LPYLLDLELF
560 570 580 590 600
LGKKILLTPM YDSLVDVMKK RWIDEGKPEY QFQPPKLSYT KRLVLGSCPR
610 620 630 640 650
LDVEGLGDLY KNLMSSNFDL QLAKKTAMKA EKLYYRTRTS ASPESLKRSK
660 670 680 690 700
EIISSGWDAQ NAFFGKNEEK EKLDFLAKLQ NRRNKETVFE FTRNPDDEMA
710 720 730 740 750
VFMKRRRKQL APIQRKATER RELLEKERMA GLSHSIEDEI LKGDDGETGY
760 770 780 790 800
TVSEDALKEF EDADQLLEAQ ENENKKKKKP KSFKDPTFFL SHYAPAGDIQ
810 820 830 840 850
DKQLQITNGF ANDAAQAAYD LNSDDKVQVH KQTATVKWDK KRKKYVNTQG
860 870 880 890 900
IDNKKYIIGE SGQKIAASFR SGRFDDWSKA RNLKPLKVGS RETSIPSNLL
910 920 930 940 950
EDPSQGPAAN GRTVRGKFKH KQMKAPKMPD KHRDNYYSQK KKVEKALQSG
960 970 980 990
ISVKGYNNAP GLRSELKSTE QIRKDRIIAE KKRAKNARPS KKRKF
Length:995
Mass (Da):112,946
Last modified:July 27, 2011 - v2
Checksum:i0533013C276095DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti733 – 741SHSIEDEIL → HILSKMKFW in CAA96458 (PubMed:9046088).Curated9
Sequence conflicti733 – 741SHSIEDEIL → HILSKMKFW in CAA98590 (PubMed:9169867).Curated9
Sequence conflicti746G → V in CAA96458 (PubMed:9046088).Curated1
Sequence conflicti746G → V in CAA98590 (PubMed:9169867).Curated1
Sequence conflicti764D → H in CAA96458 (PubMed:9046088).Curated1
Sequence conflicti764D → H in CAA98590 (PubMed:9169867).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71781 Genomic DNA Translation: CAA96458.1
Z74079 Genomic DNA Translation: CAA98590.1
BK006938 Genomic DNA Translation: DAA11821.2
PIRiS67564
RefSeqiNP_010253.2, NM_001180090.2

Genome annotation databases

EnsemblFungiiYDL031W; YDL031W; YDL031W
GeneIDi851530
KEGGisce:YDL031W

Similar proteinsi

Entry informationi

Entry nameiDBP10_YEAST
AccessioniPrimary (citable) accession number: Q12389
Secondary accession number(s): D6VRW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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