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Protein

Actin-like protein ARP8

Gene

ARP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex. Exhibits low basal ATPase activity, and unable to polymerize. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi502 – 505ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • mRNA binding Source: SGD

GO - Biological processi

  • cellular response to DNA damage stimulus Source: SGD
  • chromatin remodeling Source: SGD
  • DNA duplex unwinding Source: GOC
  • double-strand break repair Source: SGD
  • mitotic recombination Source: SGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-like protein ARP8
Gene namesi
Name:ARP8
Ordered Locus Names:YOR141C
ORF Names:YOR3348C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR141C.
SGDiS000005667. ARP8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • Ino80 complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000891281 – 881Actin-like protein ARP8Add BLAST881

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineCombined sources1
Modified residuei70PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12386.
PRIDEiQ12386.

PTM databases

iPTMnetiQ12386.

Interactioni

Subunit structurei

Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Exists as monomers and dimers, but the dimer is most probably the biologically relevant form required for stable interactions with histones that exploits the twofold symmetry of the nucleosome core (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681352EBI-2967,EBI-367540From a different organism.

Protein-protein interaction databases

BioGridi34535. 473 interactors.
DIPiDIP-6706N.
IntActiQ12386. 30 interactors.
MINTiMINT-659970.

Structurei

Secondary structure

1881
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi265 – 267Combined sources3
Beta strandi268 – 272Combined sources5
Beta strandi275 – 281Combined sources7
Beta strandi285 – 287Combined sources3
Beta strandi289 – 293Combined sources5
Beta strandi295 – 298Combined sources4
Helixi299 – 301Combined sources3
Beta strandi305 – 307Combined sources3
Helixi321 – 341Combined sources21
Helixi352 – 354Combined sources3
Beta strandi357 – 360Combined sources4
Beta strandi367 – 370Combined sources4
Helixi386 – 390Combined sources5
Turni394 – 396Combined sources3
Beta strandi397 – 400Combined sources4
Beta strandi402 – 404Combined sources3
Helixi418 – 433Combined sources16
Beta strandi434 – 437Combined sources4
Helixi442 – 447Combined sources6
Beta strandi449 – 454Combined sources6
Helixi460 – 472Combined sources13
Beta strandi477 – 483Combined sources7
Helixi484 – 491Combined sources8
Beta strandi498 – 503Combined sources6
Beta strandi508 – 514Combined sources7
Helixi520 – 522Combined sources3
Beta strandi524 – 527Combined sources4
Helixi530 – 543Combined sources14
Helixi556 – 569Combined sources14
Helixi574 – 576Combined sources3
Beta strandi579 – 586Combined sources8
Beta strandi589 – 591Combined sources3
Beta strandi593 – 602Combined sources10
Helixi603 – 609Combined sources7
Helixi610 – 612Combined sources3
Helixi615 – 619Combined sources5
Helixi628 – 631Combined sources4
Turni640 – 642Combined sources3
Helixi651 – 657Combined sources7
Helixi662 – 664Combined sources3
Helixi668 – 686Combined sources19
Helixi703 – 716Combined sources14
Helixi720 – 722Combined sources3
Helixi723 – 727Combined sources5
Beta strandi730 – 734Combined sources5
Helixi735 – 738Combined sources4
Helixi742 – 753Combined sources12
Turni757 – 759Combined sources3
Helixi763 – 778Combined sources16
Beta strandi786 – 788Combined sources3
Turni793 – 797Combined sources5
Helixi798 – 817Combined sources20
Helixi838 – 840Combined sources3
Helixi841 – 849Combined sources9
Helixi853 – 858Combined sources6
Helixi862 – 868Combined sources7
Helixi869 – 874Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AM6X-ray2.70A/B248-881[»]
4AM7X-ray3.25A/B248-881[»]
ProteinModelPortaliQ12386.
SMRiQ12386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 27Poly-Asp6

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000001763.
HOGENOMiHOG000246531.
InParanoidiQ12386.
KOiK11673.
OMAiERMRYYK.
OrthoDBiEOG092C25I4.

Family and domain databases

InterProiIPR004000. Actin.
IPR027668. Arp8/plant_Arp9.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 4 hits.
PTHR11937:SF13. PTHR11937:SF13. 4 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQEEAESSI IYEEPIDIPL EDDDDEDELE EENSVPLSSQ ADQENAENES
60 70 80 90 100
DDSVDNVVGS ETPRSVTGLS VDPRDVADEE DEDEEGEDED EDEDDNDVDN
110 120 130 140 150
EDENDNDNAN ENENELGSSR DKRAPPAVQT SKRYKKYPKL DPAKAPPGKK
160 170 180 190 200
VPLHLLEKRR LGRIKAAEEF AKTLKKIGIE KVETTTLPAT GLFQPLMLIN
210 220 230 240 250
QKNYSSDYLK KDDQIFALRD RKFLRNNNTS QISSTNTPDV IDLKSLPHSE
260 270 280 290 300
ASAAPLNDEI DLNDPTATIV IHPGSNSIKI GFPKDDHPVV VPNCVAVPKK
310 320 330 340 350
WLDLENSEHV ENVCLQREQS EEFNNIKSEM EKNFRERMRY YKRKVPGNAH
360 370 380 390 400
EQVVSFNENS KPEIISEKND PSPIEWIFDD SKLYYGSDAL RCVDEKFVIR
410 420 430 440 450
KPFRGGSFNV KSPYYKSLAE LISDVTKLLE HALNSETLNV KPTKFNQYKV
460 470 480 490 500
VLVIPDIFKK SHVETFIRVL LTELQFQAVA IIQESLATCY GAGISTSTCV
510 520 530 540 550
VNIGAAETRI ACVDEGTVLE HSAITLDYGG DDITRLFALF LLQSDFPLQD
560 570 580 590 600
WKIDSKHGWL LAERLKKNFT TFQDADVAVQ LYNFMNRSPN QPTEKYEFKL
610 620 630 640 650
FDEVMLAPLA LFFPQIFKLI RTSSHKNSSL EFQLPESRDL FTNELNDWNS
660 670 680 690 700
LSQFESKEGN LYCDLNDDLK ILNRILDAHN IIDQLQDKPE NYGNTLKENF
710 720 730 740 750
APLEKAIVQS IANASITADV TRMNSFYSNI LIVGGSSKIP ALDFILTDRI
760 770 780 790 800
NIWRPSLLSS ASFPQFYKKL TKEIKDLEGH YVNAPDKTED ENKQILQAQI
810 820 830 840 850
KEKIVEELEE QHQNIEHQNG NEHIFPVSII PPPRDMNPAL IIWKGASVLA
860 870 880
QIKLVEELFI TNSDWDVHGS RILQYKCIFT Y
Length:881
Mass (Da):100,209
Last modified:November 1, 1996 - v1
Checksum:i8174851B6B077A19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64058.1.
Z75049 Genomic DNA. Translation: CAA99341.1.
BK006948 Genomic DNA. Translation: DAA10913.1.
PIRiS67026.
RefSeqiNP_014784.1. NM_001183560.1.

Genome annotation databases

EnsemblFungiiYOR141C; YOR141C; YOR141C.
GeneIDi854309.
KEGGisce:YOR141C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64058.1.
Z75049 Genomic DNA. Translation: CAA99341.1.
BK006948 Genomic DNA. Translation: DAA10913.1.
PIRiS67026.
RefSeqiNP_014784.1. NM_001183560.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AM6X-ray2.70A/B248-881[»]
4AM7X-ray3.25A/B248-881[»]
ProteinModelPortaliQ12386.
SMRiQ12386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34535. 473 interactors.
DIPiDIP-6706N.
IntActiQ12386. 30 interactors.
MINTiMINT-659970.

PTM databases

iPTMnetiQ12386.

Proteomic databases

MaxQBiQ12386.
PRIDEiQ12386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR141C; YOR141C; YOR141C.
GeneIDi854309.
KEGGisce:YOR141C.

Organism-specific databases

EuPathDBiFungiDB:YOR141C.
SGDiS000005667. ARP8.

Phylogenomic databases

GeneTreeiENSGT00390000001763.
HOGENOMiHOG000246531.
InParanoidiQ12386.
KOiK11673.
OMAiERMRYYK.
OrthoDBiEOG092C25I4.

Enzyme and pathway databases

BioCyciYEAST:G3O-33662-MONOMER.

Miscellaneous databases

PROiQ12386.

Family and domain databases

InterProiIPR004000. Actin.
IPR027668. Arp8/plant_Arp9.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 4 hits.
PTHR11937:SF13. PTHR11937:SF13. 4 hits.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARP8_YEAST
AccessioniPrimary (citable) accession number: Q12386
Secondary accession number(s): D6W2J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.