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Q12386

- ARP8_YEAST

UniProt

Q12386 - ARP8_YEAST

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Protein

Actin-like protein ARP8

Gene

ARP8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably involved in transcription regulation via its interaction with the INO80 complex, a chromatin remodeling complex. Exhibits low basal ATPase activity, and unable to polymerize. Strongly prefer nucleosomes and H3-H4 tetramers over H2A-H2B dimers, suggesting it may act as a nucleosome recognition module within the complex.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi502 – 5054ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. mRNA binding Source: SGD

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: SGD
  2. chromatin remodeling Source: SGD
  3. DNA duplex unwinding Source: GOC
  4. double-strand break repair Source: SGD
  5. mitotic recombination Source: SGD
  6. regulation of transcription, DNA-templated Source: UniProtKB-KW
  7. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-like protein ARP8
Gene namesi
Name:ARP8
Ordered Locus Names:YOR141C
ORF Names:YOR3348C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR141c.
SGDiS000005667. ARP8.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-KW
  3. Ino80 complex Source: SGD
  4. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 881881Actin-like protein ARP8PRO_0000089128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651Phosphoserine3 Publications
Modified residuei70 – 701Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12386.
PaxDbiQ12386.
PeptideAtlasiQ12386.

Expressioni

Gene expression databases

GenevestigatoriQ12386.

Interactioni

Subunit structurei

Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Exists as monomers and dimers, but the dimer is most probably the biologically relevant form required for stable interactions with histones that exploits the twofold symmetry of the nucleosome core (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ACTA1P681352EBI-2967,EBI-367540From a different organism.

Protein-protein interaction databases

BioGridi34535. 469 interactions.
DIPiDIP-6706N.
IntActiQ12386. 30 interactions.
MINTiMINT-659970.
STRINGi4932.YOR141C.

Structurei

Secondary structure

1
881
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi265 – 2673Combined sources
Beta strandi268 – 2725Combined sources
Beta strandi275 – 2817Combined sources
Beta strandi285 – 2873Combined sources
Beta strandi289 – 2935Combined sources
Beta strandi295 – 2984Combined sources
Helixi299 – 3013Combined sources
Beta strandi305 – 3073Combined sources
Helixi321 – 34121Combined sources
Helixi352 – 3543Combined sources
Beta strandi357 – 3604Combined sources
Beta strandi367 – 3704Combined sources
Helixi386 – 3905Combined sources
Turni394 – 3963Combined sources
Beta strandi397 – 4004Combined sources
Beta strandi402 – 4043Combined sources
Helixi418 – 43316Combined sources
Beta strandi434 – 4374Combined sources
Helixi442 – 4476Combined sources
Beta strandi449 – 4546Combined sources
Helixi460 – 47213Combined sources
Beta strandi477 – 4837Combined sources
Helixi484 – 4918Combined sources
Beta strandi498 – 5036Combined sources
Beta strandi508 – 5147Combined sources
Helixi520 – 5223Combined sources
Beta strandi524 – 5274Combined sources
Helixi530 – 54314Combined sources
Helixi556 – 56914Combined sources
Helixi574 – 5763Combined sources
Beta strandi579 – 5868Combined sources
Beta strandi589 – 5913Combined sources
Beta strandi593 – 60210Combined sources
Helixi603 – 6097Combined sources
Helixi610 – 6123Combined sources
Helixi615 – 6195Combined sources
Helixi628 – 6314Combined sources
Turni640 – 6423Combined sources
Helixi651 – 6577Combined sources
Helixi662 – 6643Combined sources
Helixi668 – 68619Combined sources
Helixi703 – 71614Combined sources
Helixi720 – 7223Combined sources
Helixi723 – 7275Combined sources
Beta strandi730 – 7345Combined sources
Helixi735 – 7384Combined sources
Helixi742 – 75312Combined sources
Turni757 – 7593Combined sources
Helixi763 – 77816Combined sources
Beta strandi786 – 7883Combined sources
Turni793 – 7975Combined sources
Helixi798 – 81720Combined sources
Helixi838 – 8403Combined sources
Helixi841 – 8499Combined sources
Helixi853 – 8586Combined sources
Helixi862 – 8687Combined sources
Helixi869 – 8746Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AM6X-ray2.70A/B248-881[»]
4AM7X-ray3.25A/B248-881[»]
ProteinModelPortaliQ12386.
SMRiQ12386. Positions 259-881.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 276Poly-Asp

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiCOG5277.
GeneTreeiENSGT00390000001763.
HOGENOMiHOG000246531.
InParanoidiQ12386.
KOiK11673.
OMAiTFQDADI.
OrthoDBiEOG71RXT9.

Family and domain databases

InterProiIPR004000. Actin-related.
IPR027668. Arp8/plant_Arp9.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF13. PTHR11937:SF13. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12386-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQEEAESSI IYEEPIDIPL EDDDDEDELE EENSVPLSSQ ADQENAENES
60 70 80 90 100
DDSVDNVVGS ETPRSVTGLS VDPRDVADEE DEDEEGEDED EDEDDNDVDN
110 120 130 140 150
EDENDNDNAN ENENELGSSR DKRAPPAVQT SKRYKKYPKL DPAKAPPGKK
160 170 180 190 200
VPLHLLEKRR LGRIKAAEEF AKTLKKIGIE KVETTTLPAT GLFQPLMLIN
210 220 230 240 250
QKNYSSDYLK KDDQIFALRD RKFLRNNNTS QISSTNTPDV IDLKSLPHSE
260 270 280 290 300
ASAAPLNDEI DLNDPTATIV IHPGSNSIKI GFPKDDHPVV VPNCVAVPKK
310 320 330 340 350
WLDLENSEHV ENVCLQREQS EEFNNIKSEM EKNFRERMRY YKRKVPGNAH
360 370 380 390 400
EQVVSFNENS KPEIISEKND PSPIEWIFDD SKLYYGSDAL RCVDEKFVIR
410 420 430 440 450
KPFRGGSFNV KSPYYKSLAE LISDVTKLLE HALNSETLNV KPTKFNQYKV
460 470 480 490 500
VLVIPDIFKK SHVETFIRVL LTELQFQAVA IIQESLATCY GAGISTSTCV
510 520 530 540 550
VNIGAAETRI ACVDEGTVLE HSAITLDYGG DDITRLFALF LLQSDFPLQD
560 570 580 590 600
WKIDSKHGWL LAERLKKNFT TFQDADVAVQ LYNFMNRSPN QPTEKYEFKL
610 620 630 640 650
FDEVMLAPLA LFFPQIFKLI RTSSHKNSSL EFQLPESRDL FTNELNDWNS
660 670 680 690 700
LSQFESKEGN LYCDLNDDLK ILNRILDAHN IIDQLQDKPE NYGNTLKENF
710 720 730 740 750
APLEKAIVQS IANASITADV TRMNSFYSNI LIVGGSSKIP ALDFILTDRI
760 770 780 790 800
NIWRPSLLSS ASFPQFYKKL TKEIKDLEGH YVNAPDKTED ENKQILQAQI
810 820 830 840 850
KEKIVEELEE QHQNIEHQNG NEHIFPVSII PPPRDMNPAL IIWKGASVLA
860 870 880
QIKLVEELFI TNSDWDVHGS RILQYKCIFT Y
Length:881
Mass (Da):100,209
Last modified:November 1, 1996 - v1
Checksum:i8174851B6B077A19
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64058.1.
Z75049 Genomic DNA. Translation: CAA99341.1.
BK006948 Genomic DNA. Translation: DAA10913.1.
PIRiS67026.
RefSeqiNP_014784.1. NM_001183560.1.

Genome annotation databases

EnsemblFungiiYOR141C; YOR141C; YOR141C.
GeneIDi854309.
KEGGisce:YOR141C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64058.1 .
Z75049 Genomic DNA. Translation: CAA99341.1 .
BK006948 Genomic DNA. Translation: DAA10913.1 .
PIRi S67026.
RefSeqi NP_014784.1. NM_001183560.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AM6 X-ray 2.70 A/B 248-881 [» ]
4AM7 X-ray 3.25 A/B 248-881 [» ]
ProteinModelPortali Q12386.
SMRi Q12386. Positions 259-881.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34535. 469 interactions.
DIPi DIP-6706N.
IntActi Q12386. 30 interactions.
MINTi MINT-659970.
STRINGi 4932.YOR141C.

Proteomic databases

MaxQBi Q12386.
PaxDbi Q12386.
PeptideAtlasi Q12386.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR141C ; YOR141C ; YOR141C .
GeneIDi 854309.
KEGGi sce:YOR141C.

Organism-specific databases

CYGDi YOR141c.
SGDi S000005667. ARP8.

Phylogenomic databases

eggNOGi COG5277.
GeneTreei ENSGT00390000001763.
HOGENOMi HOG000246531.
InParanoidi Q12386.
KOi K11673.
OMAi TFQDADI.
OrthoDBi EOG71RXT9.

Enzyme and pathway databases

BioCyci YEAST:G3O-33662-MONOMER.

Miscellaneous databases

NextBioi 976325.
PROi Q12386.

Gene expression databases

Genevestigatori Q12386.

Family and domain databases

InterProi IPR004000. Actin-related.
IPR027668. Arp8/plant_Arp9.
[Graphical view ]
PANTHERi PTHR11937. PTHR11937. 1 hit.
PTHR11937:SF13. PTHR11937:SF13. 1 hit.
Pfami PF00022. Actin. 1 hit.
[Graphical view ]
SMARTi SM00268. ACTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family."
    Poch O., Winsor B.
    Yeast 13:1053-1058(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  5. "Involvement of actin-related proteins in ATP-dependent chromatin remodeling."
    Shen X., Ranallo R., Choi E., Wu C.
    Mol. Cell 12:147-155(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE INO80 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65 AND SER-70, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Interactions between the nucleosome histone core and Arp8 in the INO80 chromatin remodeling complex."
    Saravanan M., Wuerges J., Bose D., McCormack E.A., Cook N.J., Zhang X., Wigley D.B.
    Proc. Natl. Acad. Sci. U.S.A. 109:20883-20888(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 248-881, ELECTRON MICROSCOPY, FUNCTION, SUBUNIT.

Entry informationi

Entry nameiARP8_YEAST
AccessioniPrimary (citable) accession number: Q12386
Secondary accession number(s): D6W2J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3