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Protein

1-acylglycerol-3-phosphate O-acyltransferase ICT1

Gene

ICT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Lysophosphatidic acid acyltransferase involved in membrane remodeling leading to increased organic solvent tolerance. Involved in resistance to azoles and copper.4 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

GO - Molecular functioni

GO - Biological processi

  • phosphatidic acid biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism, Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-32249-MONOMER.
ReactomeiR-SCE-1483152. Hydrolysis of LPE.

Protein family/group databases

ESTHERiyeast-ict1. CGI-58_ABHD5_ABHD4.
MEROPSiS33.A43.

Names & Taxonomyi

Protein namesi
Recommended name:
1-acylglycerol-3-phosphate O-acyltransferase ICT1 (EC:2.3.1.51)
Alternative name(s):
Increased copper tolerance protein 1
Lysophosphatidic acid acyltransferase ICT1
Short name:
LPAAT
Gene namesi
Name:ICT1
Ordered Locus Names:YLR099C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR099C.
SGDiS000004089. ICT1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3943941-acylglycerol-3-phosphate O-acyltransferase ICT1PRO_0000080854Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi31372. 9 interactions.
DIPiDIP-5245N.
IntActiQ12385. 1 interaction.
MINTiMINT-554368.

Structurei

3D structure databases

ProteinModelPortaliQ12385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi374 – 3796HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016277.
HOGENOMiHOG000065957.
InParanoidiQ12385.
OMAiDAGHNLF.
OrthoDBiEOG793BKF.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 2 hits.

Sequencei

Sequence statusi: Complete.

Q12385-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWTNTFKWCS KTEKETTTAD AKVCASVQGL KALQQQIMDS TTVRGSVNNT
60 70 80 90 100
MTPGGINQWH FHNKRANKVC TPTVLIHGYA ASSMAFYRTF ENLSDNIKDL
110 120 130 140 150
YAIDLPANGA SEAPALQVNK TKKIKSLRFK HIEDDVVIPV IEKRPPAEDI
160 170 180 190 200
KSHLEQYESY FVDRIEQWRK DNKLRKINVV GHSFGGYISF KYALKYPDSI
210 220 230 240 250
EKLCLISPLG VENSIHAITH KWEPNTTYPL TFTDPSSRYY TRKLNVPRFI
260 270 280 290 300
FENQLNVLKW MGPIGSKLCS NYISTAYVKV PDQIYKDYLL HSFVGKNQTV
310 320 330 340 350
QPQTIKVFTH LFERNLIARD PIINNVRFLN PATPVMFMYG EHDWMDKYAG
360 370 380 390
YLTTESMLKN KAKASYVEVP DAGHNLFLDN PQHFASSLVS FLSK
Length:394
Mass (Da):45,141
Last modified:November 1, 1996 - v1
Checksum:i7B686E611E81C380
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73271 Genomic DNA. Translation: CAA97663.1.
Z73272 Genomic DNA. Translation: CAA97666.1.
U53876 Genomic DNA. Translation: AAB67543.1.
BK006945 Genomic DNA. Translation: DAA09415.1.
PIRiS64933.
RefSeqiNP_013200.1. NM_001181986.1.

Genome annotation databases

EnsemblFungiiYLR099C; YLR099C; YLR099C.
GeneIDi850788.
KEGGisce:YLR099C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73271 Genomic DNA. Translation: CAA97663.1.
Z73272 Genomic DNA. Translation: CAA97666.1.
U53876 Genomic DNA. Translation: AAB67543.1.
BK006945 Genomic DNA. Translation: DAA09415.1.
PIRiS64933.
RefSeqiNP_013200.1. NM_001181986.1.

3D structure databases

ProteinModelPortaliQ12385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31372. 9 interactions.
DIPiDIP-5245N.
IntActiQ12385. 1 interaction.
MINTiMINT-554368.

Protein family/group databases

ESTHERiyeast-ict1. CGI-58_ABHD5_ABHD4.
MEROPSiS33.A43.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR099C; YLR099C; YLR099C.
GeneIDi850788.
KEGGisce:YLR099C.

Organism-specific databases

EuPathDBiFungiDB:YLR099C.
SGDiS000004089. ICT1.

Phylogenomic databases

GeneTreeiENSGT00390000016277.
HOGENOMiHOG000065957.
InParanoidiQ12385.
OMAiDAGHNLF.
OrthoDBiEOG793BKF.

Enzyme and pathway databases

BioCyciYEAST:G3O-32249-MONOMER.
ReactomeiR-SCE-1483152. Hydrolysis of LPE.

Miscellaneous databases

PROiQ12385.

Family and domain databases

Gene3Di3.40.50.1820. 3 hits.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Drug-induced phenotypes provide a tool for the functional analysis of yeast genes."
    Launhardt H., Hinnen A., Munder T.
    Yeast 14:935-942(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: FUNCTION.
  5. "Screening of genes involved in isooctane tolerance in Saccharomyces cerevisiae by using mRNA differential display."
    Miura S., Zou W., Ueda M., Tanaka A.
    Appl. Environ. Microbiol. 66:4883-4889(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "YLR099C (ICT1) encodes a soluble Acyl-CoA-dependent lysophosphatidic acid acyltransferase responsible for enhanced phospholipid synthesis on organic solvent stress in Saccharomyces cerevisiae."
    Ghosh A.K., Ramakrishnan G., Rajasekharan R.
    J. Biol. Chem. 283:9768-9775(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOMAIN.

Entry informationi

Entry nameiICT1_YEAST
AccessioniPrimary (citable) accession number: Q12385
Secondary accession number(s): D6VY99, Q7LGX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The isooctane tolerance of organic-solvent tolerant strain KK-21 may result from the alteration of the expression of several genes including ICT1, due to the loss of their proper regulation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.