Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CTP-dependent diacylglycerol kinase 1

Gene

DGK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-tRNA splicing (By similarity). CTP-dependent diacylglycerol kinase that catalyzes the phosphorylation of diacylglycerol (DAG) to phosphatidate (PA). Controls phosphatidate levels at the nuclear envelope. Counteracts the activity of PAH1/SMP2. Involved in the resistance to nickel chloride and nalidixic acid. May be involved in vesicle trafficking between the endoplasmic reticulum and the Golgi apparatus.By similarity3 Publications

Catalytic activityi

CTP + 1,2-diacyl-sn-glycerol = CDP + 1,2-diacyl-sn-glycerol 3-phosphate.2 Publications

Cofactori

Ca2+1 Publication, Mg2+1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide, dCTP, and sphingoid bases including sphinganine, sphingosine and phytosphingosine. DAG pyrophosphate, cardiolipin, CDP-DAG, and lyso-PA inhibited activity by 23-66%. Also inhibited by Ca2+ concentrations of more than 1 mM, by addition of EDTA or EGTA at 5 mM, and by 5 mM Mn2+ and Zn2+. Stimulated by major membrane phospholipids including phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol, and phosphatidate. Also stimulated to a maximum by addition of TritonX-100 at a concentration of 1 mM, followed by an apparent inhibition of activity at concentrations above 1 mM.1 Publication

Kineticsi

  1. KM=0.3 mM for CTP1 Publication
  2. KM=0.4 mM for dCTP1 Publication
  1. Vmax=0.018 nM/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.0-7.5.1 Publication

Temperature dependencei

Maximum activity at 30 degrees Celsius. Labile above 40 degrees Celsius.1 Publication

GO - Molecular functioni

  • diacylglycerol kinase activity Source: SGD

GO - Biological processi

  • phosphatidic acid biosynthetic process Source: SGD
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

ER-Golgi transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33794-MONOMER.
YEAST:G3O-33794-MONOMER.
BRENDAi2.7.1.B4. 984.
SABIO-RKQ12382.

Chemistry databases

SwissLipidsiSLP:000000046.

Names & Taxonomyi

Protein namesi
Recommended name:
CTP-dependent diacylglycerol kinase 1 (EC:2.7.1.174)
Alternative name(s):
Diglyceride kinase 1
Short name:
DAG kinase 1
High-copy suppressor of SLY1 defect protein 1
Gene namesi
Name:DGK1
Synonyms:HSD1
Ordered Locus Names:YOR311C
ORF Names:O6111
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR311C.
SGDiS000005838. DGK1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 77LumenalSequence analysisAdd BLAST77
Transmembranei78 – 95HelicalSequence analysisAdd BLAST18
Topological domaini96 – 103CytoplasmicSequence analysis8
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Topological domaini125 – 140LumenalSequence analysisAdd BLAST16
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 163CytoplasmicSequence analysis2
Transmembranei164 – 184HelicalSequence analysisAdd BLAST21
Topological domaini185 – 203LumenalSequence analysisAdd BLAST19
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Topological domaini225 – 244CytoplasmicSequence analysisAdd BLAST20
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 290LumenalSequence analysisAdd BLAST25

GO - Cellular componenti

  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76R → A: Loss of kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure. 1 Publication1
Mutagenesisi77K → A: Loss of kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure. 1 Publication1
Mutagenesisi177D → A: No kinase activity. Not temperature-sensitive for growth. Does not trigger nuclear membrane expansion. 2 Publications1
Mutagenesisi184G → A: 70% reduction in kinase activity. Not temperature-sensitive for growth. Regular shaped nuclear membrane structure. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002403821 – 290CTP-dependent diacylglycerol kinase 1Add BLAST290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi11N-linked (GlcNAc...)Sequence analysis1
Modified residuei44PhosphoserineCombined sources1
Modified residuei45PhosphoserineCombined sources1
Modified residuei46PhosphoserineCombined sources1
Glycosylationi197N-linked (GlcNAc...)Sequence analysis1
Glycosylationi270N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ12382.
PRIDEiQ12382.

PTM databases

iPTMnetiQ12382.

Interactioni

Protein-protein interaction databases

BioGridi34699. 221 interactors.
DIPiDIP-5021N.
MINTiMINT-561696.

Structurei

3D structure databases

ProteinModelPortaliQ12382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DGK1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000198978.
InParanoidiQ12382.
KOiK16368.
OMAiHLKSHEW.
OrthoDBiEOG092C3R29.

Sequencei

Sequence statusi: Complete.

Q12382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTEDAIALP NSTLEPRTEA KQRLSSKSHQ VSAKVTIPAK EEISSSDDDA
60 70 80 90 100
HVPVTEIHLK SHEWFGDFIT KHEIPRKVFH SSIGFITLYL YTQGINYKNV
110 120 130 140 150
LWPLIYAFII LFILDLIRLN WPFFNMLYCR TVGALMRKKE IHTYNGVLWY
160 170 180 190 200
ILGLIFSFNF FSKDVTLISL FLLSWSDTAA ATIGRKYGHL TPKVARNKSL
210 220 230 240 250
AGSIAAFTVG VITCWVFYGY FVPAYSYVNK PGEIQWSPET SRLSLNMLSL
260 270 280 290
LGGVVAALSE GIDLFNWDDN FTIPVLSSLF MNAVIKTFKK
Length:290
Mass (Da):32,840
Last modified:November 1, 1996 - v1
Checksum:iF8A0EE6F1018025B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90565 Genomic DNA. Translation: CAA62166.1.
Z75219 Genomic DNA. Translation: CAA99631.1.
BK006948 Genomic DNA. Translation: DAA11076.1.
PIRiS58323.
RefSeqiNP_014956.3. NM_001183731.3.

Genome annotation databases

EnsemblFungiiYOR311C; YOR311C; YOR311C.
GeneIDi854488.
KEGGisce:YOR311C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X90565 Genomic DNA. Translation: CAA62166.1.
Z75219 Genomic DNA. Translation: CAA99631.1.
BK006948 Genomic DNA. Translation: DAA11076.1.
PIRiS58323.
RefSeqiNP_014956.3. NM_001183731.3.

3D structure databases

ProteinModelPortaliQ12382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34699. 221 interactors.
DIPiDIP-5021N.
MINTiMINT-561696.

Chemistry databases

SwissLipidsiSLP:000000046.

PTM databases

iPTMnetiQ12382.

Proteomic databases

MaxQBiQ12382.
PRIDEiQ12382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR311C; YOR311C; YOR311C.
GeneIDi854488.
KEGGisce:YOR311C.

Organism-specific databases

EuPathDBiFungiDB:YOR311C.
SGDiS000005838. DGK1.

Phylogenomic databases

HOGENOMiHOG000198978.
InParanoidiQ12382.
KOiK16368.
OMAiHLKSHEW.
OrthoDBiEOG092C3R29.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33794-MONOMER.
YEAST:G3O-33794-MONOMER.
BRENDAi2.7.1.B4. 984.
SABIO-RKQ12382.

Miscellaneous databases

PROiQ12382.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiDGK1_YEAST
AccessioniPrimary (citable) accession number: Q12382
Secondary accession number(s): D6W310
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.1 Publication
Rescues the lethality of dephosphorylated PAH1/SMP2. Overexpression causes the appearance of phosphatidate-enriched membranes around the nucleus, leading to expansion of the nuclear membrane without proliferation of the cortical endoplasmic reticulum membrane. Deletion restores normal nuclear structure in PAH1/SMP2 deleted cells and returns the level of INO1 mRNA to normal. Deletion does not affect the abnormal levels of phosphatidylinositol and major neutral lipid triacylglycerol seen in the PAH1/SMP2 deletion mutant.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.