Autophagy protein 5 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

Q12380 (ATG5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Autophagy protein 5

Gene names

Name:	ATG5
Synonyms:	APG5
Ordered Locus Names:	YPL149W
ORF Names:	P2601

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cytoplasm to vacuole transport (Cvt) and autophagy vesicles formation. Required for ATG8 association to the vesicle membranes. Ref.1 Ref.5 Ref.6 Ref.7 Ref.8 Ref.10
Subunit structure	Conjugated with ATG12. The ATG5-ATG12 conjugate forms a complex with several units of ATG16.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. Note: Found in pre-autophagosomal structure and other punctate structures. Ref.9
Post-translational modification	Conjugated to ATG12; which is essential for autophagy. Conjugation with ATG12 involves ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme.
Miscellaneous	Present with 606 molecules/cell in log phase SD medium. Ref.13 Small amount of ATG5-ATG12 conjugate is enough to perform normal autophagy.
Sequence similarities	Belongs to the ATG5 family.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm Membrane
Molecular function	Hydrolase
PTM	Isopeptide bond Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	C-terminal protein lipidation Inferred from direct assay. Source: SGD CVT pathway Inferred from mutant phenotype Ref.8. Source: SGD macroautophagy Inferred from mutant phenotype Ref.1. Source: SGD mitochondrion degradation Inferred from mutant phenotype. Source: SGD piecemeal microautophagy of nucleus Inferred from mutant phenotype. Source: SGD
Cellular component	Atg12-Atg5-Atg16 complex Inferred from direct assay Ref.11. Source: SGD autophagic vacuole Inferred from direct assay. Source: SGD cytosol Inferred from direct assay Ref.11. Source: SGD pre-autophagosomal structure membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.7. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATG12	P38316	8	EBI-2664,EBI-2692
ATG16	Q03818	5	EBI-2664,EBI-27344

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 294

294

Autophagy protein 5

PRO_0000219010

Amino acid modifications

Cross-link

149

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)

Experimental info

Mutagenesis

149

K → R: Loss of conjugation. Ref.6

Secondary structure

294

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q12380 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BE513A631DEAA0A1
Show »

FASTA

294

33,560

        10         20         30         40         50         60 
MNDIKQLLWN GELNVLVSID PSFLMKGSPR EIAVLRIRVP RETYLVNYMP LIWNKIKSFL 

        70         80         90        100        110        120 
SFDPLTDSEK YFWFEHNKTP IPWNYPVGVL FDCLAGKSAT FTTSFENQVK DVLTFLRIHL 

       130        140        150        160        170        180 
VMGDSLPPTI IPIASSKTQA EKFWFHQWKQ VCFILNGSSK AIMSLSVNEA RKFWGSVITR 

       190        200        210        220        230        240 
NFQDFIEISN KISSSRPRHI PLIIQTSRTS GTFRISQPTI SMTGVNPTLK DIEGDILDVK 

       250        260        270        280        290 
EGINGNDVMV ICQGIEIPWH MLLYDLYSKL RSFDGFLYIT LVPIKGGDKA SSEL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and functional analyses of APG5, a gene involved in autophagy in yeast." Kametaka S., Matsuura A., Wada Y., Ohsumi Y. Gene 178:139-143(1996) [PubMed: 8921905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae." Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993) [PubMed: 8224160] [Abstract] Cited for: FUNCTION.
[6]	"A protein conjugation system essential for autophagy." Mizushima N., Noda T., Yoshimori T., Tanaka Y., Ishii T., George M.D., Klionsky D.J., Ohsumi M., Ohsumi Y. Nature 395:395-398(1998) [PubMed: 9759731] [Abstract] Cited for: FUNCTION, CONJUGATION TO ATG12, MUTAGENESIS OF LYS-149. Strain: ATCC 26109 / X2180.
[7]	"Apg16p is required for the function of the Apg12p-Apg5p conjugate in the yeast autophagy pathway." Mizushima N., Noda T., Ohsumi Y. EMBO J. 18:3888-3896(1999) [PubMed: 10406794] [Abstract] Cited for: FUNCTION, INTERACTION WITH ATG12 AND ATG16.
[8]	"Apg5p functions in the sequestration step in the cytoplasm-to-vacuole targeting and macroautophagy pathways." George M.D., Baba M., Scott S.V., Mizushima N., Garrison B.S., Ohsumi Y., Klionsky D.J. Mol. Biol. Cell 11:969-982(2000) [PubMed: 10712513] [Abstract] Cited for: FUNCTION.
[9]	"The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation." Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y. EMBO J. 20:5971-5981(2001) [PubMed: 11689437] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex." Kim J., Huang W.-P., Klionsky D.J. J. Cell Biol. 152:51-64(2001) [PubMed: 11149920] [Abstract] Cited for: FUNCTION.
[11]	"Formation of the approximately 350-kDa Apg12-Apg5.Apg16 multimeric complex, mediated by Apg16 oligomerization, is essential for autophagy in yeast." Kuma A., Mizushima N., Ishihara N., Ohsumi Y. J. Biol. Chem. 277:18619-18625(2002) [PubMed: 11897782] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH ATG12 AND ATG16.
[12]	"A unified nomenclature for yeast autophagy-related genes." Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y., Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y. Dev. Cell 5:539-545(2003) [PubMed: 14536056] [Abstract] Cited for: NOMENCLATURE.
[13]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D83519 Genomic DNA. Translation: BAA11937.1.
X96770 Genomic DNA. Translation: CAA65572.1.
Z73505 Genomic DNA. Translation: CAA97854.1.
BK006949 Genomic DNA. Translation: DAA11286.1.

PIR

S65160.

RefSeq

NP_015176.1. NM_001183963.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DYM	X-ray	2.20	A/C/E/G	1-294	[»]
2DYO	X-ray	1.97	A	1-294	[»]

ProteinModelPortal

Q12380.

SMR

Q12380. Positions 1-285.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1195N.

IntAct

Q12380. 9 interactions.

MINT

MINT-388341.

STRING

Q12380.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YPL149W; YPL149W; YPL149W.

GeneID

855954.

KEGG

sce:YPL149W.

NMPDR

fig|4932.3.peg.6306.

Organism-specific databases

CYGD

YPL149w.

SGD

S000006070. ATG5.

Phylogenomic databases

eggNOG

fuNOG11001.

GeneTree

EFGT00050000005329.

OMA

LWNGSIN.

OrthoDB

EOG4NW2V5.

Gene expression databases

ArrayExpress

Q12380.

Genevestigator

Q12380.

GermOnline

YPL149W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR007239. Autophagy-rel_prot_5.
[Graphical view]

K08339.

PANTHER

PTHR13040. APG5. 1 hit.

Pfam

PF04106. APG5. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

980741.

Entry information

Entry name

ATG5_YEAST

Accession

Primary (citable) accession number: Q12380
Secondary accession number(s): D6W3M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents