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Protein

Autophagy protein 5

Gene

ATG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy). Also required for mitophagy, which eliminates defective or superfluous mitochondria in order to fulfill cellular energy requirements and prevent excess ROS production. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes. ATG12-ATG5 rearranges the ATG3 catalytic center and enhances its E2 activity. Plays a role in the regulation of filamentous growth and chronological longevity.17 Publications

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.1 Publication
Small amount of ATG5-ATG12 conjugate is enough to perform normal autophagy.

GO - Molecular functioni

  • enzyme activator activity Source: SGD
  • hydrolase activity Source: UniProtKB-KW

GO - Biological processi

  • autophagosome assembly Source: GO_Central
  • autophagy of mitochondrion Source: SGD
  • cellular response to nitrogen starvation Source: GO_Central
  • C-terminal protein lipidation Source: SGD
  • late nucleophagy Source: SGD
  • macroautophagy Source: SGD
  • piecemeal microautophagy of the nucleus Source: SGD
  • protein localization by the Cvt pathway Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processAutophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34046-MONOMER
ReactomeiR-SCE-1632852 Macroautophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy protein 5
Gene namesi
Name:ATG5
Synonyms:APG5
Ordered Locus Names:YPL149W
ORF Names:P2601
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YPL149W
SGDiS000006070 ATG5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi149K → R: Loss of conjugation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190101 – 294Autophagy protein 5Add BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki149Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ATG12)

Post-translational modificationi

Conjugated to ATG12; which is essential for autophagy. Conjugation with ATG12 involves ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ12380
PaxDbiQ12380
PRIDEiQ12380

Interactioni

Subunit structurei

Conjugated with ATG12. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. The ATG12-ATG5 conjugate associates also with ATG3.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36034, 153 interactors
DIPiDIP-1195N
IntActiQ12380, 30 interactors
MINTiQ12380
STRINGi4932.YPL149W

Structurei

Secondary structure

1294
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1 – 9Combined sources9
Beta strandi12 – 19Combined sources8
Helixi21 – 23Combined sources3
Helixi30 – 33Combined sources4
Beta strandi34 – 43Combined sources10
Helixi45 – 48Combined sources4
Helixi49 – 56Combined sources8
Helixi57 – 59Combined sources3
Beta strandi60 – 62Combined sources3
Turni64 – 66Combined sources3
Beta strandi71 – 76Combined sources6
Beta strandi79 – 81Combined sources3
Beta strandi83 – 85Combined sources3
Helixi87 – 95Combined sources9
Helixi96 – 98Combined sources3
Turni105 – 107Combined sources3
Beta strandi115 – 125Combined sources11
Beta strandi136 – 138Combined sources3
Helixi139 – 156Combined sources18
Beta strandi157 – 159Combined sources3
Helixi160 – 163Combined sources4
Helixi167 – 179Combined sources13
Helixi182 – 192Combined sources11
Beta strandi202 – 205Combined sources4
Beta strandi208 – 212Combined sources5
Helixi230 – 232Combined sources3
Helixi233 – 236Combined sources4
Turni239 – 241Combined sources3
Beta strandi248 – 252Combined sources5
Beta strandi255 – 257Combined sources3
Helixi263 – 270Combined sources8
Beta strandi277 – 283Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DYMX-ray2.20A/C/E/G1-294[»]
2DYOX-ray1.97A1-294[»]
3W1SX-ray2.60A1-284[»]
ProteinModelPortaliQ12380
SMRiQ12380
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12380

Family & Domainsi

Sequence similaritiesi

Belongs to the ATG5 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000004766
InParanoidiQ12380
KOiK08339
OMAiWHYPIGV
OrthoDBiEOG092C3XV6

Family and domain databases

InterProiView protein in InterPro
IPR007239 Atg5
PANTHERiPTHR13040 PTHR13040, 1 hit
PfamiView protein in Pfam
PF04106 APG5, 1 hit

Sequencei

Sequence statusi: Complete.

Q12380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDIKQLLWN GELNVLVSID PSFLMKGSPR EIAVLRIRVP RETYLVNYMP
60 70 80 90 100
LIWNKIKSFL SFDPLTDSEK YFWFEHNKTP IPWNYPVGVL FDCLAGKSAT
110 120 130 140 150
FTTSFENQVK DVLTFLRIHL VMGDSLPPTI IPIASSKTQA EKFWFHQWKQ
160 170 180 190 200
VCFILNGSSK AIMSLSVNEA RKFWGSVITR NFQDFIEISN KISSSRPRHI
210 220 230 240 250
PLIIQTSRTS GTFRISQPTI SMTGVNPTLK DIEGDILDVK EGINGNDVMV
260 270 280 290
ICQGIEIPWH MLLYDLYSKL RSFDGFLYIT LVPIKGGDKA SSEL
Length:294
Mass (Da):33,560
Last modified:November 1, 1996 - v1
Checksum:iBE513A631DEAA0A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83519 Genomic DNA Translation: BAA11937.1
X96770 Genomic DNA Translation: CAA65572.1
Z73505 Genomic DNA Translation: CAA97854.1
BK006949 Genomic DNA Translation: DAA11286.1
PIRiS65160
RefSeqiNP_015176.1, NM_001183963.1

Genome annotation databases

EnsemblFungiiYPL149W; YPL149W; YPL149W
GeneIDi855954
KEGGisce:YPL149W

Similar proteinsi

Entry informationi

Entry nameiATG5_YEAST
AccessioniPrimary (citable) accession number: Q12380
Secondary accession number(s): D6W3M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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