Q12379 (SWM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaphase-promoting complex subunit SWM1

Alternative name(s):
Anaphase-promoting complex subunit 13
Spore wall maturation protein 1

Gene names

Name:	SWM1
Synonyms:	APC13
Ordered Locus Names:	YDR260C
ORF Names:	YD9320A.11, YD9320A.11c

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication. SWM1 is required for APC/C activity in meiosis. Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. SWM1 interacts directly with CDC23 and APC5, and is required to tether APC9, CDC16, CDC26 and CDC27 to the complex. Ref.4 Ref.5 Ref.6
Sequence similarities	Belongs to the APC13 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis Ubl conjugation pathway
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from physical interaction Ref.5. Source: SGD ascospore wall assembly Inferred from mutant phenotype. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein ubiquitination Inferred from physical interaction Ref.5. Source: SGD regulation of mitotic metaphase/anaphase transition Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	anaphase-promoting complex Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APC4	Q04601	5	EBI-33330,EBI-32842
APC5	Q08683	4	EBI-33330,EBI-35371
CDC23	P16522	5	EBI-33330,EBI-4216

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	Anaphase-promoting complex subunit SWM1		PRO_0000072352
Regions
	Compositional bias	2 – 5	4	Poly-Ser

Sequences

	Sequence		Length	Mass (Da)
	Q12379 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7DD5FC4CBDE8BF19	FASTA	170	19,356
	10 20 30 40 50 60 MSSSSYRDSY FQYRHLPAPH HILYAEWNQD ILALPDEVAN ITMAMKDNTR TDAEEGRAPQ 70 80 90 100 110 120 DGERNSNVRE SAQGKALMTS EQNSNRYWNS FHDEDDWNLF NGMELESNGV VTFAGQAFDH 130 140 150 160 170 SLNGGTNSRN DGANEPRKET ITGSIFDRRI TQLAYARNNG WHELALPQSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes." Yoon H.-J., Feoktistova A., Wolfe B.A., Jennings J.L., Link A.J., Gould K.L. Curr. Biol. 12:2048-2054(2002) [PubMed: 12477395] [Abstract] Cited for: SUBUNIT.
[5]	"Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex." Hall M.C., Torres M.P., Schroeder G.K., Borchers C.H. J. Biol. Chem. 278:16698-16705(2003) [PubMed: 12609981] [Abstract] Cited for: SUBUNIT, INTERACTION WITH APC5 AND CDC23.
[6]	"Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27." Schwickart M., Havlis J., Habermann B., Bogdanova A., Camasses A., Oelschlaegel T., Shevchenko A., Zachariae W. Mol. Cell. Biol. 24:3562-3576(2004) [PubMed: 15060174] [Abstract] Cited for: FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z70202 Genomic DNA. Translation: CAA94099.1. Z68329 Genomic DNA. Translation: CAA92718.1. AY557727 Genomic DNA. Translation: AAS56053.1. BK006938 Genomic DNA. Translation: DAA12100.1.
PIR	S67318.
RefSeq	NP_010546.1. NM_001180568.1.
3D structure databases
ProteinModelPortal	Q12379.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4818N.
IntAct	Q12379. 21 interactions.
MINT	MINT-514556.
STRING	Q12379.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR260C; YDR260C; YDR260C.
GeneID	851847.
KEGG	sce:YDR260C.
NMPDR	fig|4932.3.peg.1304.
Organism-specific databases
CYGD	YDR260c.
SGD	S000002668. SWM1.
Phylogenomic databases
eggNOG	fuNOG15658.
OMA	VANITMA.
OrthoDB	EOG4ZW8MW.
Gene expression databases
ArrayExpress	Q12379.
Genevestigator	Q12379.
GermOnline	YDR260C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR008401. Apc13p. [Graphical view]
KO	K12416.
Pfam	PF05839. Apc13p. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969763.

Entry information

Entry name

SWM1_YEAST

Accession

Primary (citable) accession number: Q12379
Secondary accession number(s): D6VSP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program