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Protein

26S proteasome regulatory subunit RPN6

Gene

RPN6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly.3 Publications

Miscellaneous

Present with 16800 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural molecule activity Source: SGD

GO - Biological processi

  • proteasome assembly Source: UniProtKB
  • ubiquitin-dependent protein catabolic process Source: UniProtKB

Enzyme and pathway databases

BioCyciYEAST:G3O-29500-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-187577 SCF(Skp2)-mediated degradation of p27/p21
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6798695 Neutrophil degranulation
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN6
Alternative name(s):
Proteasome non-ATPase subunit 4
Gene namesi
Name:RPN6
Synonyms:NAS4
Ordered Locus Names:YDL097C
ORF Names:D2381
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YDL097C
SGDiS000002255 RPN6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi132F → L in rpn6-2; temperature-sensitive mutant that shows defects in proteasome assembly when incubated at 37 degrees Celsius; when associated with P-377. 1 Publication1
Mutagenesisi377L → P in rpn6-2; temperature-sensitive mutant that shows defects in proteasome assembly when incubated at 37 degrees Celsius; when associated with L-132. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001738602 – 43426S proteasome regulatory subunit RPN6Add BLAST433

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1 Publication1

Post-translational modificationi

N-acetylated by NAT1.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ12377
PaxDbiQ12377
PRIDEiQ12377

PTM databases

iPTMnetiQ12377

Interactioni

Subunit structurei

Component of the lid subcomplex of the 19S proteasome regulatory particle complex (also named PA700 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31965, 693 interactors
DIPiDIP-1581N
IntActiQ12377, 39 interactors
MINTiQ12377
STRINGi4932.YDL097C

Structurei

Secondary structure

1434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi55 – 57Combined sources3
Helixi59 – 64Combined sources6
Turni65 – 68Combined sources4
Helixi71 – 83Combined sources13
Turni84 – 88Combined sources5
Helixi94 – 105Combined sources12
Beta strandi106 – 109Combined sources4
Helixi112 – 127Combined sources16
Helixi131 – 144Combined sources14
Turni145 – 148Combined sources4
Helixi152 – 168Combined sources17
Helixi171 – 187Combined sources17
Helixi192 – 207Combined sources16
Helixi212 – 228Combined sources17
Helixi232 – 248Combined sources17
Helixi254 – 272Combined sources19
Helixi276 – 281Combined sources6
Turni284 – 287Combined sources4
Helixi288 – 291Combined sources4
Helixi294 – 308Combined sources15
Helixi311 – 316Combined sources6
Turni317 – 321Combined sources5
Helixi322 – 325Combined sources4
Helixi329 – 351Combined sources23
Beta strandi354 – 356Combined sources3
Helixi359 – 366Combined sources8
Helixi370 – 382Combined sources13
Beta strandi388 – 391Combined sources4
Turni392 – 395Combined sources4
Beta strandi396 – 399Combined sources4
Helixi407 – 428Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J47electron microscopy-Q407-431[»]
3JCKelectron microscopy3.50C1-434[»]
3JCOelectron microscopy4.80Q1-434[»]
3JCPelectron microscopy4.60Q1-434[»]
4CR2electron microscopy7.70Q1-434[»]
4CR3electron microscopy9.30Q1-434[»]
4CR4electron microscopy8.80Q1-434[»]
5A5Belectron microscopy9.50Q1-434[»]
5MPBelectron microscopy7.80Q1-434[»]
5MPCelectron microscopy7.70Q1-434[»]
5MPDelectron microscopy4.10Q1-434[»]
5MPEelectron microscopy4.50Q1-434[»]
5WVIelectron microscopy6.30Q1-434[»]
5WVKelectron microscopy4.20Q1-434[»]
ProteinModelPortaliQ12377
SMRiQ12377
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini235 – 404PCIPROSITE-ProRule annotationAdd BLAST170

Sequence similaritiesi

Belongs to the proteasome subunit S9 family.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063301
HOGENOMiHOG000210093
InParanoidiQ12377
KOiK03036
OMAiKDFNTAF
OrthoDBiEOG092C330M

Family and domain databases

InterProiView protein in InterPro
IPR000717 PCI_dom
IPR011990 TPR-like_helical_dom_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48452 SSF48452, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPGSKLEE ARRLVNEKQY NEAEQVYLSL LDKDSSQSSA AAGASVDDKR
60 70 80 90 100
RNEQETSILE LGQLYVTMGA KDKLREFIPH STEYMMQFAK SKTVKVLKTL
110 120 130 140 150
IEKFEQVPDS LDDQIFVCEK SIEFAKREKR VFLKHSLSIK LATLHYQKKQ
160 170 180 190 200
YKDSLALIND LLREFKKLDD KPSLVDVHLL ESKVYHKLRN LAKSKASLTA
210 220 230 240 250
ARTAANSIYC PTQTVAELDL MSGILHCEDK DYKTAFSYFF ESFESYHNLT
260 270 280 290 300
THNSYEKACQ VLKYMLLSKI MLNLIDDVKN ILNAKYTKET YQSRGIDAMK
310 320 330 340 350
AVAEAYNNRS LLDFNTALKQ YEKELMGDEL TRSHFNALYD TLLESNLCKI
360 370 380 390 400
IEPFECVEIS HISKIIGLDT QQVEGKLSQM ILDKIFYGVL DQGNGWLYVY
410 420 430
ETPNQDATYD SALELVGQLN KVVDQLFEKA SVLY
Length:434
Mass (Da):49,774
Last modified:January 23, 2007 - v3
Checksum:iA8B1F7E6F73A4024
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95644 Genomic DNA Translation: CAA64916.1
Z74145 Genomic DNA Translation: CAA98664.1
BK006938 Genomic DNA Translation: DAA11763.1
PIRiS67639
RefSeqiNP_010186.1, NM_001180156.1

Genome annotation databases

EnsemblFungiiYDL097C; YDL097C; YDL097C
GeneIDi851461
KEGGisce:YDL097C

Entry informationi

Entry nameiRPN6_YEAST
AccessioniPrimary (citable) accession number: Q12377
Secondary accession number(s): D6VRQ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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