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Protein

Mitochondrial ornithine transporter 1

Gene

ORT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for arginine biosynthesis. Transports ornithine synthesized from glutamate in the mitochondrial matrix to the cytosol, where it is converted to arginine.1 Publication

GO - Molecular functioni

  • L-ornithine transmembrane transporter activity Source: SGD

GO - Biological processi

  • arginine biosynthetic process Source: SGD
  • L-ornithine transmembrane transport Source: GOC
  • mitochondrial ornithine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33654-MONOMER.
ReactomeiR-SCE-70635. Urea cycle.

Protein family/group databases

TCDBi2.A.29.9.2. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial ornithine transporter 1
Gene namesi
Name:ORT1
Synonyms:ARG11
Ordered Locus Names:YOR130C
ORF Names:O3299, YOR3299C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR130C.
SGDiS000005656. ORT1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei69 – 8921Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei104 – 12421Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei171 – 18717Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei213 – 23321Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei267 – 28721Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial envelope Source: SGD
  • mitochondrial inner membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292Mitochondrial ornithine transporter 1PRO_0000090684Add
BLAST

Proteomic databases

MaxQBiQ12375.

Interactioni

Protein-protein interaction databases

BioGridi34525. 22 interactions.
DIPiDIP-8953N.
MINTiMINT-2493498.

Structurei

3D structure databases

ProteinModelPortaliQ12375.
SMRiQ12375. Positions 17-292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 9787Solcar 1Add
BLAST
Repeati105 – 19692Solcar 2Add
BLAST
Repeati211 – 29282Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000110966.
HOGENOMiHOG000168307.
InParanoidiQ12375.
KOiK15114.
OMAiYRHEGIR.
OrthoDBiEOG7673MT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSKKKGLI EGAILDIING SIAGACGKVI EFPFDTVKVR LQTQASNVFP
60 70 80 90 100
TTWSCIKFTY QNEGIARGFF QGIASPLVGA CLENATLFVS YNQCSKFLEK
110 120 130 140 150
HTNVSPLGQI LISGGVAGSC ASLVLTPVEL VKCKLQVANL QVASAKTKHT
160 170 180 190 200
KVLPTIKAII TERGLAGLWQ GQSGTFIRES FGGVAWFATY EIVKKSLKDR
210 220 230 240 250
HSLDDPKRDE SKIWELLISG GSAGLAFNAS IFPADTVKSV MQTEHISLTN
260 270 280 290
AVKKIFGKFG LKGFYRGLGI TLFRAVPANA AVFYIFETLS AL
Length:292
Mass (Da):31,520
Last modified:September 21, 2011 - v2
Checksum:i3B80DDCC5875FD66
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051S → F in CAA62118 (PubMed:8904341).Curated
Sequence conflicti105 – 1051S → F in CAA64049 (PubMed:9200815).Curated
Sequence conflicti105 – 1051S → F in CAA99329 (PubMed:9169874).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87414 Genomic DNA. Translation: CAA60862.1.
X90518 Genomic DNA. Translation: CAA62118.1.
X94335 Genomic DNA. Translation: CAA64049.1.
Z75038 Genomic DNA. Translation: CAA99329.1.
BK006948 Genomic DNA. Translation: DAA10904.2.
PIRiS60997.
RefSeqiNP_014773.4. NM_001183549.4.

Genome annotation databases

EnsemblFungiiYOR130C; YOR130C; YOR130C.
GeneIDi854297.
KEGGisce:YOR130C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87414 Genomic DNA. Translation: CAA60862.1.
X90518 Genomic DNA. Translation: CAA62118.1.
X94335 Genomic DNA. Translation: CAA64049.1.
Z75038 Genomic DNA. Translation: CAA99329.1.
BK006948 Genomic DNA. Translation: DAA10904.2.
PIRiS60997.
RefSeqiNP_014773.4. NM_001183549.4.

3D structure databases

ProteinModelPortaliQ12375.
SMRiQ12375. Positions 17-292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34525. 22 interactions.
DIPiDIP-8953N.
MINTiMINT-2493498.

Protein family/group databases

TCDBi2.A.29.9.2. the mitochondrial carrier (mc) family.

Proteomic databases

MaxQBiQ12375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR130C; YOR130C; YOR130C.
GeneIDi854297.
KEGGisce:YOR130C.

Organism-specific databases

EuPathDBiFungiDB:YOR130C.
SGDiS000005656. ORT1.

Phylogenomic databases

GeneTreeiENSGT00730000110966.
HOGENOMiHOG000168307.
InParanoidiQ12375.
KOiK15114.
OMAiYRHEGIR.
OrthoDBiEOG7673MT.

Enzyme and pathway databases

BioCyciYEAST:G3O-33654-MONOMER.
ReactomeiR-SCE-70635. Urea cycle.

Miscellaneous databases

NextBioi976296.
PROiQ12375.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The ARG11 gene of Saccharomyces cerevisiae encodes a mitochondrial integral membrane protein required for arginine biosynthesis."
    Crabeel M., Soetens O., de Rijcke M., Pratiwi R., Pankiewicz R.
    J. Biol. Chem. 271:25011-25018(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "Sequencing and analysis of 51 kb on the right arm of chromosome XV from Saccharomyces cerevisiae reveals 30 open reading frames."
    Wiemann S., Rechmann S., Benes V., Voss H., Schwager C., Vlcek C., Stegemann J., Zimmermann J., Erfle H., Paces V., Ansorge W.
    Yeast 12:281-288(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 105.
    Strain: ATCC 204508 / S288c.
  6. "Identification of the yeast ARG-11 gene as a mitochondrial ornithine carrier involved in arginine biosynthesis."
    Palmieri L., De Marco V., Iacobazzi V., Palmieri F., Runswick M.J., Walker J.E.
    FEBS Lett. 410:447-451(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiORT1_YEAST
AccessioniPrimary (citable) accession number: Q12375
Secondary accession number(s): D6W2I8, Q92273
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 21, 2011
Last modified: May 11, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.