Q12373 (HIF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HAT1-interacting factor 1

Gene names

Name:	HIF1
Ordered Locus Names:	YLL022C
ORF Names:	L1205

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone H3 and H4 specific chaperone component of the nuclear histone acetyltransferase B (HAT-B) complex. Involved in chromatin assembly and telomere silencing. Ref.6 Ref.7
Subunit structure	Component of the nuclear histone acetyltransferase B (HAT-B) complex composed of at least HAT1, HAT2 and HIF1. Interacts with histones H3 and H4 in a HAT1/HAT2 dependent manner. Ref.6 Ref.7
Subcellular location	Nucleus Ref.4 Ref.6 Ref.7.
Miscellaneous	Present with 4550 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the NASP family. Contains 2 TPR repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat TPR repeat
Molecular function	Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histone acetylation Inferred from direct assay Ref.6. Source: SGD nucleosome assembly Inferred from direct assay Ref.7. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	histone acetyltransferase complex Inferred from direct assay Ref.6Ref.7. Source: SGD
Molecular function	histone binding Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HAT2	P39984	5	EBI-31911,EBI-8185
HHF1	P02309	5	EBI-31911,EBI-8113

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	HAT1-interacting factor 1		PRO_0000227731
Regions
	Repeat	186 – 220	35	TPR 1
	Repeat	229 – 262	34	TPR 2
	Compositional bias	97 – 184	88	Glu-rich
Amino acid modifications
	Modified residue	174	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q12373 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: B280CFCCB6896506	FASTA	385	43,428
	10 20 30 40 50 60 MKLRAEDVLA NGTSRHKVQI DMERQVQIAK DLLAQKKFLE AAKRCQQTLD SLPKDGLLPD 70 80 90 100 110 120 PELFTIFAQA VYNMEVQNSG NLFGDALLAG DDGSGSESES EPESDVSNGE EGNENGQTEI 130 140 150 160 170 180 PNSRMFQFDQ EEEDLTGDVD SGDSEDSGEG SEEEEENVEK EEERLALHEL ANFSPANEHD 190 200 210 220 230 240 DEIEDVSQLR KSGFHIYFEN DLYENALDLL AQALMLLGRP TADGQSLTEN SRLRIGDVYI 250 260 270 280 290 300 LMGDIEREAE MFSRAIHHYL KALGYYKTLK PAEQVTEKVI QAEFLVCDAL RWVDQVPAKD 310 320 330 340 350 360 KLKRFKHAKA LLEKHMTTRP KDSELQQARL AQIQDDIDEV QENQQHGSKR PLSQPTTSIG 370 380 FPALEKPLGD FNDLSQLVKK KPRRH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of 32kb on the left arm of yeast chromosome XII reveals six known genes, a new member of the seripauperins family and a new ABC transporter homologous to the human multidrug resistance protein." Purnelle B., Goffeau A. Yeast 13:183-188(1997) [PubMed: 9046100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Hif1 is a component of yeast histone acetyltransferase B, a complex mainly localized in the nucleus." Poveda A., Pamblanco M., Tafrov S., Tordera V., Sternglanz R., Sendra R. J. Biol. Chem. 279:16033-16043(2004) [PubMed: 14761951] [Abstract] Cited for: IDENTIFICATION IN THE NUCLEAR HAT-B COMPLEX, FUNCTION OF THE HAT-B COMPLEX, INTERACTION WITH HISTONE H4, SUBCELLULAR LOCATION.
[7]	"The nuclear Hat1p/Hat2p complex: a molecular link between type B histone acetyltransferases and chromatin assembly." Ai X., Parthun M.R. Mol. Cell 14:195-205(2004) [PubMed: 15099519] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE NUCLEAR HAT-B COMPLEX, MASS SPECTROMETRY, INTERACTION WITH HISTONES H3 AND H4, SUBCELLULAR LOCATION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-174, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97560 Genomic DNA. Translation: CAA66169.1. Z73127 Genomic DNA. Translation: CAA97470.1. BK006945 Genomic DNA. Translation: DAA09298.1.
PIR	S64770.
RefSeq	NP_013078.1. NM_001181842.1.
3D structure databases
ProteinModelPortal	Q12373.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6442N.
IntAct	Q12373. 8 interactions.
MINT	MINT-473929.
STRING	Q12373.
Proteomic databases
PeptideAtlas	Q12373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLL022C; YLL022C; YLL022C.
GeneID	850638.
KEGG	sce:YLL022C.
NMPDR	fig|4932.3.peg.4069.
Organism-specific databases
CYGD	YLL022c.
SGD	S000003945. HIF1.
Phylogenomic databases
OrthoDB	EOG4P8JTZ.
Gene expression databases
ArrayExpress	Q12373.
Genevestigator	Q12373.
GermOnline	YLL022C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019544. Tetratricopeptide_SHNi-TPR_dom. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 2 hits.
KO	K11372.
Pfam	PF10516. SHNi-TPR. 1 hit. [Graphical view]
PROSITE	PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	966567.

Entry information

Entry name

HIF1_YEAST

Accession

Primary (citable) accession number: Q12373
Secondary accession number(s): D6VXY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program