Q12365 (BBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 82.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Spindle pole component BBP1 Alternative name(s): BFR1-binding protein 1 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 385 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Component of the spindle pole body (SPB) required for insertion of the nascent SPB into the nuclear envelope and for the proper execution of spindle pole body (SPB) duplication. Connects the central plaque of the SPB with the half-bridge. Required for proper localization of CDC5 at the SPB and for proper M-phase progression. Ref.5 Ref.7 |
| Subunit structure | |
| Subcellular location | Cytoplasm › cytoskeleton › spindle pole body. Note: Associates with the periphary of the central plaque. Ref.5 Ref.7 |
| Domain | The C-ter coiled-coil domain is sufficient for localization and homodimerization. Ref.7 |
| Miscellaneous | Present with 922 molecules/cell in log phase SD medium. Ref.6 |
| Sequence similarities | Belongs to the BBP1 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm Cytoskeleton Nucleus |
| Domain | Coiled coil |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | microtubule nucleation Inferred from physical interaction. Source: SGD spindle pole body duplication in nuclear envelopeInferred from mutant phenotype Ref.5. Source: SGD |
| Cellular component | nuclear envelope Inferred from mutant phenotype Ref.5. Source: GOC spindle pole bodyInferred from direct assay. Source: SGD |
| Molecular function | protein binding Inferred from physical interaction Ref.5. Source: IntAct structural constituent of cytoskeletonInferred from physical interaction. Source: SGD |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| MPS2 | P53159 | 6 | EBI-3448,EBI-23834 | |
| SPC29 | P33419 | 3 | EBI-3448,EBI-12041 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 385 | 385 | Spindle pole component BBP1 | PRO_0000064840 | |||||
Regions | |||||||||
| Coiled coil | 229 – 355 | 127 | Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 71 | 1 | Phosphoserine Ref.10 | ||||||
| Modified residue | 73 | 1 | Phosphoserine Ref.8 Ref.10 | ||||||
| Modified residue | 115 | 1 | Phosphoserine Ref.9 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | Xue Z., Shan X., Melese T. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. Hani J.Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication." Schramm C., Elliott S., Shevchenko A., Schiebel E. EMBO J. 19:421-433(2000) [PubMed: 10654940] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR1; MPS2 AND SPC29. |
| [6] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [7] | "Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae." Park C.J., Song S., Giddings T.H. Jr., Ro H.S., Sakchaisri K., Park J.E., Seong Y.S., Winey M., Lee K.S. Mol. Biol. Cell 15:1711-1723(2004) [PubMed: 14767068] [Abstract] Cited for: DOMAIN, SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, INTERACTION WITH CDC5. |
| [8] | "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY. Strain: YAL6B. |
| [9] | "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY. Strain: ADR376. |
| [10] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71 AND SER-73, MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X92658 Genomic DNA. Translation: CAA63347.1. Z73610 Genomic DNA. Translation: CAA97980.1. Z73611 Genomic DNA. Translation: CAA97981.1. AY693019 Genomic DNA. Translation: AAT93038.1. BK006949 Genomic DNA. Translation: DAA11182.1. |
| PIR | S61566. |
| RefSeq | NP_015068.1. NM_001184069.1. |
3D structure databases | |
| ProteinModelPortal | Q12365. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-4418N. |
| IntAct | Q12365. 19 interactions. |
| MINT | MINT-512290. |
| STRING | Q12365. |
Proteomic databases | |
| PRIDE | Q12365. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YPL255W; YPL255W; YPL255W. |
| GeneID | 855820. |
| KEGG | sce:YPL255W. |
| NMPDR | fig|4932.3.peg.6194. |
Organism-specific databases | |
| CYGD | YPL255w. |
| SGD | S000006176. BBP1. |
Phylogenomic databases | |
| eggNOG | fuNOG11882. |
| OMA | LPGKFPS. |
| OrthoDB | EOG41VPCN. |
Gene expression databases | |
| ArrayExpress | Q12365. |
| Genevestigator | Q12365. |
| GermOnline | YPL255W. Saccharomyces cerevisiae. |
Family and domain databases | |
| ProtoNet | Search... |
Other | |
| NextBio | 980362. |
Entry information
| Entry name | BBP1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q12365 Secondary accession number(s): D6W3B6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| SIMILARITY comments Index of protein domains and families |

Clusters with