Q12365 (BBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spindle pole component BBP1

Alternative name(s):
BFR1-binding protein 1

Gene names

Name:	BBP1
Ordered Locus Names:	YPL255W
ORF Names:	P0745

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the spindle pole body (SPB) required for insertion of the nascent SPB into the nuclear envelope and for the proper execution of spindle pole body (SPB) duplication. Connects the central plaque of the SPB with the half-bridge. Required for proper localization of CDC5 at the SPB and for proper M-phase progression. Ref.5 Ref.7
Subunit structure	Homodimer. Interacts with KAR1, MPS2 and SPC29. Ref.5 Ref.7
Subcellular location	Cytoplasm › cytoskeleton › spindle pole body. Note: Associates with the periphary of the central plaque. Ref.5 Ref.7
Domain	The C-ter coiled-coil domain is sufficient for localization and homodimerization. Ref.7
Miscellaneous	Present with 922 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the BBP1 family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	microtubule nucleation Inferred from physical interaction. Source: SGD spindle pole body duplication in nuclear envelope Inferred from mutant phenotype Ref.5. Source: SGD
Cellular component	nuclear envelope Inferred from mutant phenotype Ref.5. Source: GOC spindle pole body Inferred from direct assay. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct structural constituent of cytoskeleton Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MPS2	P53159	6	EBI-3448,EBI-23834
SPC29	P33419	3	EBI-3448,EBI-12041

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 385	385	Spindle pole component BBP1		PRO_0000064840
Regions
	Coiled coil	229 – 355	127	Potential
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.10
	Modified residue	73	1	Phosphoserine Ref.8 Ref.10
	Modified residue	115	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q12365 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 3A1A827E7664E4E0	FASTA	385	45,384
	10 20 30 40 50 60 MNQEDNTGGG GIFGLFKWTK DALFGTDISP SMKYKDQEER RDRSRYAQDD TNFSMKFGND 70 80 90 100 110 120 SNRRSTNLSR SNSWSGLDST LHRKYELLPE YNENGFNSIV NGDHHSKERI RSLRSPAPIV 130 140 150 160 170 180 PREPLRNEPT DTFGHRLHTK RRTINELSNS QIPFIPPQED DPLLSKLFNK DGVNEVRRSP 190 200 210 220 230 240 YKLSVKDIPG KFPSPLTKRD EIDNYYVRDE DACHKNREYK KAYFDLFAQM DLNSRDLEDL 250 260 270 280 290 300 CEDVREQREQ FHRNEQTYKQ AYEEMRAELV NELKKSKTLF ENYYSLGQKY KSLKKVLDQT 310 320 330 340 350 360 ISHEAELATS RERLYQEEDL KNFEIQTLKQ RLSDLELKYT NLQIEKDMQR DNYESEIHDL 370 380 LLQLSLRNNE RKDTSAGSNI FSTGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Xue Z., Shan X., Melese T. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication." Schramm C., Elliott S., Shevchenko A., Schiebel E. EMBO J. 19:421-433(2000) [PubMed: 10654940] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR1; MPS2 AND SPC29.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Requirement for Bbp1p in the proper mitotic functions of Cdc5p in Saccharomyces cerevisiae." Park C.J., Song S., Giddings T.H. Jr., Ro H.S., Sakchaisri K., Park J.E., Seong Y.S., Winey M., Lee K.S. Mol. Biol. Cell 15:1711-1723(2004) [PubMed: 14767068] [Abstract] Cited for: DOMAIN, SUBCELLULAR LOCATION, SUBUNIT, FUNCTION, INTERACTION WITH CDC5.
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-73, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY. Strain: ADR376.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71 AND SER-73, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92658 Genomic DNA. Translation: CAA63347.1. Z73610 Genomic DNA. Translation: CAA97980.1. Z73611 Genomic DNA. Translation: CAA97981.1. AY693019 Genomic DNA. Translation: AAT93038.1. BK006949 Genomic DNA. Translation: DAA11182.1.
PIR	S61566.
RefSeq	NP_015068.1. NM_001184069.1.
3D structure databases
ProteinModelPortal	Q12365.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4418N.
IntAct	Q12365. 19 interactions.
MINT	MINT-512290.
STRING	Q12365.
Proteomic databases
PRIDE	Q12365.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL255W; YPL255W; YPL255W.
GeneID	855820.
KEGG	sce:YPL255W.
NMPDR	fig|4932.3.peg.6194.
Organism-specific databases
CYGD	YPL255w.
SGD	S000006176. BBP1.
Phylogenomic databases
eggNOG	fuNOG11882.
OMA	LPGKFPS.
OrthoDB	EOG41VPCN.
Gene expression databases
ArrayExpress	Q12365.
Genevestigator	Q12365.
GermOnline	YPL255W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	980362.

Entry information

Entry name

BBP1_YEAST

Accession

Primary (citable) accession number: Q12365
Secondary accession number(s): D6W3B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program