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Protein

Bifunctional protein RIB2

Gene

RIB2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for synthesis of pseudouridine from uracil-32 in cytoplasmic transfer RNAs.
Involved in riboflavin biosynthesis. Converts 2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate.

Miscellaneous

Present with 1540 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

tRNA uridine(32) = tRNA pseudouridine(32).1 Publication
2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H2O = 5-amino-6-(5-phosphoribosylamino)uracil + NH3.1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Pathwayi: riboflavin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. GTP cyclohydrolase-2 (RIB1)
  2. Bifunctional protein RIB2 (RIB2)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-6-(D-ribitylamino)uracil from GTP, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei211By similarity1
Metal bindingi482Zinc; catalyticBy similarity1
Active sitei484Proton donorBy similarity1
Metal bindingi515Zinc; catalyticBy similarity1
Metal bindingi525Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

  • enzyme-directed rRNA pseudouridine synthesis Source: GO_Central
  • riboflavin biosynthetic process Source: SGD
  • tRNA pseudouridine synthesis Source: SGD

Keywordsi

Molecular functionHydrolase, Isomerase, RNA-binding
Biological processRiboflavin biosynthesis, tRNA processing
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YOL066C-MONOMER
YEAST:YOL066C-MONOMER
BRENDAi5.4.99.28 984
UniPathwayiUPA00275; UER00401

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein RIB2
Including the following 2 domains:
tRNA pseudouridine(32) synthase, cytoplasmic (EC:5.4.99.28)
Alternative name(s):
tRNA pseudouridine synthase 8
tRNA pseudouridylate synthase 8
tRNA-uridine isomerase 8
Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC:3.5.4.26)
Short name:
DRAP deaminase
Alternative name(s):
Riboflavin-specific deaminase
Gene namesi
Name:RIB2
Synonyms:PUS8
Ordered Locus Names:YOL066C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL066C
SGDiS000005427 RIB2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001627251 – 591Bifunctional protein RIB2Add BLAST591

Proteomic databases

MaxQBiQ12362
PaxDbiQ12362
PRIDEiQ12362

Interactioni

Protein-protein interaction databases

BioGridi34335, 173 interactors
DIPiDIP-3840N
STRINGi4932.YOL066C

Structurei

3D structure databases

ProteinModelPortaliQ12362
SMRiQ12362
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 168S4 RNA-bindingPROSITE-ProRule annotationAdd BLAST70
Domaini433 – 552CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST120

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 432tRNA pseudouridine synthaseAdd BLAST432
Regioni433 – 591DRAP deaminaseAdd BLAST159

Sequence similaritiesi

In the N-terminal section; belongs to the pseudouridine synthase RluA family.Curated
In the C-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.Curated

Phylogenomic databases

GeneTreeiENSGT00420000029802
HOGENOMiHOG000167783
InParanoidiQ12362
KOiK14655
OMAiQCALEKY
OrthoDBiEOG092C23UC

Family and domain databases

InterProiView protein in InterPro
IPR002125 CMP_dCMP_dom
IPR016193 Cytidine_deaminase-like
IPR020103 PsdUridine_synth_cat_dom_sf
IPR006225 PsdUridine_synth_RluC/D
IPR006224 PsdUridine_synth_RluC/D_CS
IPR006145 PsdUridine_synth_RsuA/RluD
IPR002942 S4_RNA-bd
PfamiView protein in Pfam
PF00383 dCMP_cyt_deam_1, 1 hit
PF00849 PseudoU_synth_2, 1 hit
SUPFAMiSSF53927 SSF53927, 1 hit
SSF55120 SSF55120, 2 hits
TIGRFAMsiTIGR00005 rluA_subfam, 1 hit
PROSITEiView protein in PROSITE
PS51747 CYT_DCMP_DEAMINASES_2, 1 hit
PS01129 PSI_RLU, 1 hit
PS50889 S4, 1 hit

Sequencei

Sequence statusi: Complete.

Q12362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDSNNEASD DFNNLLNKEI ESAKEVKLRK FANRNNNRNE NSSKVKDASG
60 70 80 90 100
FRLRVIQTDG HKTKKTDPDY EVTIDGPLRK IEPYFFTYKT FCKERWRDRK
110 120 130 140 150
LVDVFVSEFR DREPSYYSKT IAEGKVYLND EPANLDTIIR DGDLITHKVH
160 170 180 190 200
RHEPPVTSKP IDIVFEDEDI LVIDKPSSIP VHPTGRYRFN TITKMLERQL
210 220 230 240 250
GYSVHPCNRL DKPTSGLMFL AKTPLGADRM GDQMKAREVT KEYVARVKGE
260 270 280 290 300
FPIGIVEVDK PVRSVNPKVA LNAVCEMSDE NAKHAKTVFQ RVSYDGQTSI
310 320 330 340 350
VKCKPLTGRT HQIRVHLQYL GFPIANDPIY SNPDIWGPDL GRGGLQNYDD
360 370 380 390 400
IVLKLDAIGK TNPAESWIHP HSEGEYLLGR QCEECEAEMY TDPGTNDLDL
410 420 430 440 450
WLHAFRYESL ERNSDTQKPL WSYRTKYPEW ALEPHRRYME MAVKEAGKCG
460 470 480 490 500
PTKTAFSVGA VLVHGTQVLA TGYSRELPGN THAEQCALIK YSQLHPNCPT
510 520 530 540 550
IVPMGTVLYT TMEPCSFRLS GNEPCCDRIL ATQGAIGTVF VGVMEPDTFV
560 570 580 590
KNNTSLNKLE SHGVNYIQIP GYEEECTIIA FKGHDNSDDK A
Length:591
Mass (Da):67,036
Last modified:November 1, 1997 - v1
Checksum:i5C625500C997ED83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z21618 Genomic DNA Translation: CAA79742.1
Z74808 Genomic DNA Translation: CAA99076.1
BK006948 Genomic DNA Translation: DAA10717.1
PIRiS50972
RefSeqiNP_014575.1, NM_001183321.1

Genome annotation databases

EnsemblFungiiYOL066C; YOL066C; YOL066C
GeneIDi854088
KEGGisce:YOL066C

Similar proteinsi

Entry informationi

Entry nameiRIB2_YEAST
AccessioniPrimary (citable) accession number: Q12362
Secondary accession number(s): D6W201
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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