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Protein

Ammonia transport outward protein 3

Gene

ATO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transporter protein required for ammonia export. Induced in rho0 cells, probably to eliminate the excess ammonia that arises because of a potential defect in ammonia assimilation in those cells.1 Publication

GO - Molecular functioni

  • acetate transmembrane transporter activity Source: GO_Central
  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD
  • plasma membrane acetate transport Source: GO_Central
  • transmembrane transport Source: SGD

Keywordsi

Biological processAmmonia transport, Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29932-MONOMER.

Protein family/group databases

TCDBi2.A.96.1.5. the acetate uptake transporter (acetr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonia transport outward protein 3
Gene namesi
Name:ATO3
Ordered Locus Names:YDR384C
ORF Names:D9481.20, D9509.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR384C.
SGDiS000002792. ATO3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 84ExtracellularSequence analysisAdd BLAST84
Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
Topological domaini106 – 120CytoplasmicSequence analysisAdd BLAST15
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 181ExtracellularSequence analysisAdd BLAST40
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
Topological domaini203 – 207CytoplasmicSequence analysis5
Transmembranei208 – 228HelicalSequence analysisAdd BLAST21
Topological domaini229 – 236ExtracellularSequence analysis8
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 275CytoplasmicSequence analysisAdd BLAST18

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001357071 – 275Ammonia transport outward protein 3Add BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ12359.
TopDownProteomicsiQ12359.

PTM databases

iPTMnetiQ12359.

Expressioni

Inductioni

Expression is under the control of the ADR1, CAT8, and GCN4 transcription factors. Also induced by external ammonia and in rho0 cells.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi32445. 20 interactors.
DIPiDIP-4513N.
IntActiQ12359. 2 interactors.
MINTiMINT-565107.
STRINGi4932.YDR384C.

Structurei

3D structure databases

ProteinModelPortaliQ12359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000006887.
HOGENOMiHOG000224167.
InParanoidiQ12359.
KOiK07034.
OMAiMTVFSSF.
OrthoDBiEOG092C3O7T.

Family and domain databases

InterProiView protein in InterPro
IPR000791. Grp1/Fun34/SatP.
PfamiView protein in Pfam
PF01184. Grp1_Fun34_YaaH. 1 hit.
PROSITEiView protein in PROSITE
PS01114. GPR1_FUN34_YAAH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q12359-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSSASSPQD LEKGVNTLEN IETLPQQGSI AGVSQGFPNI QEIYSDRDFI
60 70 80 90 100
TLGSSTYRRR DLLNALDRGD GEEGNCAKYT PHQFANPVPL GLASFSLSCL
110 120 130 140 150
VLSLINANVR GVTDGKWALS LFMFFGGAIE LFAGLLCFVI GDTYAMTVFS
160 170 180 190 200
SFGGFWICYG YGLTDTDNLV SGYTDPTMLN NVIGFFLAGW TVFTFLMLMC
210 220 230 240 250
TLKSTWGLFL LLTFLDLTFL LLCIGTFIDN NNLKMAGGYF GILSSCCGWY
260 270
SLYCSVVSPS NSYLAFRAHT MPNAP
Length:275
Mass (Da):30,028
Last modified:November 1, 1996 - v1
Checksum:iCB4E91128403C6CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28373 Genomic DNA. Translation: AAB64820.1.
U32274 Genomic DNA. Translation: AAB64826.1.
BK006938 Genomic DNA. Translation: DAA12228.1.
PIRiS61179.
RefSeqiNP_010672.1. NM_001180692.1.

Genome annotation databases

EnsemblFungiiYDR384C; YDR384C; YDR384C.
GeneIDi851992.
KEGGisce:YDR384C.

Similar proteinsi

Entry informationi

Entry nameiATO3_YEAST
AccessioniPrimary (citable) accession number: Q12359
Secondary accession number(s): D6VT18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: August 30, 2017
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names