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Protein

Cell wall mannoprotein PST1

Gene

PST1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a partially redundant function to ECM33 in cell wall integrity. May be involved in a repair mechanism activated in response to cell wall damage.2 Publications

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-29664-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall mannoprotein PST1
Alternative name(s):
Haze protective factor 2
Protoplast secreted protein 1
Gene namesi
Name:PST1
Synonyms:HPF2
Ordered Locus Names:YDR055W
ORF Names:D4214, YD9609.09
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR055W.
SGDiS000002462. PST1.

Subcellular locationi

  • Cell membrane; Lipid-anchorGPI-anchor
  • Secretedcell wall

  • Note: Identified as GPI-anchored plasma membrane protein (GPI-PMP) as well as non-covalently-linked, soluble protein of the cell wall. Secreted by regenerating protoplasts. In budded cells, concentrates at the surface of the buds.

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • extracellular region Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

Pathology & Biotechi

Biotechnological usei

Mannoprotein called haze protective factor from wine that is able to prevent visible wine protein haze formation. This mannoprotein showed haze-protective activity against wine proteins and BSA when either was heated in white wine.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000003319320 – 419Cell wall mannoprotein PST1Add BLAST400
PropeptideiPRO_0000033194420 – 444Removed in mature formSequence analysisAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Glycosylationi76N-linked (GlcNAc...)Sequence analysis1
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Glycosylationi196N-linked (GlcNAc...)Sequence analysis1
Glycosylationi210N-linked (GlcNAc...)1 Publication1
Glycosylationi228N-linked (GlcNAc...)1 Publication1
Glycosylationi235N-linked (GlcNAc...)1 Publication1
Glycosylationi242N-linked (GlcNAc...)1 Publication1
Glycosylationi263N-linked (GlcNAc...)Sequence analysis1
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Glycosylationi280N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)1 Publication1
Lipidationi419GPI-anchor amidated asparagineSequence analysis1

Post-translational modificationi

Extensively N- and O-mannosylated.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ12355.
PRIDEiQ12355.

2D gel databases

COMPLUYEAST-2DPAGEQ12355.

Expressioni

Inductioni

Positively regulated by cell integrity signaling through MPK1 in response to cell wall perturbation. Induction is dependent on transcription factor RLM1.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi32108. 14 interactors.
DIPiDIP-7811N.
MINTiMINT-2779480.

Structurei

3D structure databases

ProteinModelPortaliQ12355.
SMRiQ12355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 26Poly-Ser5
Compositional biasi356 – 416Ser-richAdd BLAST61

Sequence similaritiesi

Belongs to the SPS2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00530000065410.
HOGENOMiHOG000093252.
InParanoidiQ12355.
OMAiTQLNSID.
OrthoDBiEOG092C3DSS.

Family and domain databases

Gene3Di3.80.20.20. 2 hits.
InterProiIPR032675. L_dom-like.
IPR000494. Rcpt_L-dom.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLHSLIAST ALLITSALAA TSSSSSIPSS CTISSHATAT AQSDLDKYSR
60 70 80 90 100
CDTLVGNLTI GGGLKTGALA NVKEINGSLT IFNATNLTSF AADSLESITD
110 120 130 140 150
SLNLQSLTIL TSASFGSLQS VDSIKLITLP AISSFTSNIK SANNIYISDT
160 170 180 190 200
SLQSVDGFSA LKKVNVFNVN NNKKLTSIKS PVETVSDSLQ FSFNGNQTKI
210 220 230 240 250
TFDDLVWANN ISLTDVHSVS FANLQKINSS LGFINNSISS LNFTKLNTIG
260 270 280 290 300
QTFSIVSNDY LKNLSFSNLS TIGGALVVAN NTGLQKIGGL DNLTTIGGTL
310 320 330 340 350
EVVGNFTSLN LDSLKSVKGG ADVESKSSNF SCNALKALQK KGGIKGESFV
360 370 380 390 400
CKNGASSTSV KLSSTSKSQS SQTTAKVSKS SSKAEEKKFT SGDIKAAASA
410 420 430 440
SSVSSSGASS SSSKSSKGNA AIMAPIGQTT PLVGLLTAII MSIM
Length:444
Mass (Da):45,777
Last modified:November 1, 1996 - v1
Checksum:i230F60CACA5921A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58971.1.
Z74351 Genomic DNA. Translation: CAA98873.1.
Z49209 Genomic DNA. Translation: CAA89084.1.
BK006938 Genomic DNA. Translation: DAA11901.1.
PIRiS54039.
RefSeqiNP_010340.1. NM_001180363.1.

Genome annotation databases

EnsemblFungiiYDR055W; YDR055W; YDR055W.
GeneIDi851625.
KEGGisce:YDR055W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84162 Genomic DNA. Translation: CAA58971.1.
Z74351 Genomic DNA. Translation: CAA98873.1.
Z49209 Genomic DNA. Translation: CAA89084.1.
BK006938 Genomic DNA. Translation: DAA11901.1.
PIRiS54039.
RefSeqiNP_010340.1. NM_001180363.1.

3D structure databases

ProteinModelPortaliQ12355.
SMRiQ12355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32108. 14 interactors.
DIPiDIP-7811N.
MINTiMINT-2779480.

2D gel databases

COMPLUYEAST-2DPAGEQ12355.

Proteomic databases

MaxQBiQ12355.
PRIDEiQ12355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR055W; YDR055W; YDR055W.
GeneIDi851625.
KEGGisce:YDR055W.

Organism-specific databases

EuPathDBiFungiDB:YDR055W.
SGDiS000002462. PST1.

Phylogenomic databases

GeneTreeiENSGT00530000065410.
HOGENOMiHOG000093252.
InParanoidiQ12355.
OMAiTQLNSID.
OrthoDBiEOG092C3DSS.

Enzyme and pathway databases

BioCyciYEAST:G3O-29664-MONOMER.

Miscellaneous databases

PROiQ12355.

Family and domain databases

Gene3Di3.80.20.20. 2 hits.
InterProiIPR032675. L_dom-like.
IPR000494. Rcpt_L-dom.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPST1_YEAST
AccessioniPrimary (citable) accession number: Q12355
Secondary accession number(s): D6VS41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11700 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.