Reviewed, UniProtKB/Swiss-Prot Q12349 (ATP14_YEAST)

Names and origin

Protein names

Recommended name:
    ATP synthase subunit H, mitochondrial

Gene names

Name:	ATP14
Ordered Locus Names:	YLR295C
ORF Names:	L8003.20

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.
Subunit structure	F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. In yeast, the dimeric form of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta, epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j and k.
Subcellular location	Mitochondrion. Mitochondrion inner membrane.
Miscellaneous	Present with 6140 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	ATP synthesis Hydrogen ion transport Ion transport Transport
Cellular component	CF(0) Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	ATP synthesis coupled proton transport Ref.1 Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) Ref.1 Inferred from mutant phenotype. Source: SGD
Molecular function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P87271	1	EBI-3071,EBI-37156
	Q07535	1	EBI-3071,EBI-37019
	Q08222	1	EBI-3071,EBI-32700
ACF4	P47129	1	EBI-3071,EBI-25556
BIT61	P47041	1	EBI-3071,EBI-25889
MED8	P38304	1	EBI-3071,EBI-20932
SMT3	Q12306	1	EBI-3071,EBI-17490

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion
	Chain	33 – 124	92	ATP synthase subunit H, mitochondrial		PRO_0000002525

Sequences

	Sequence		Length	Mass (Da)
	Q12349-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7AE196CFB250ACBB	FASTA	124	14,128
	10 20 30 40 50 60 MFPIASRRIL LNASVLPLRL CNRNFTTTRI SYNVIQDLYL RELKDTKLAP STLQDAEGNV 70 80 90 100 110 120 KPWNPPQKPN LPELELQGPE ALKAYTEQNV ETAHVAKESE EGESEPIEED WLVLDDAEET KESH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATP synthase of yeast mitochondria. Isolation of the subunit h and disruption of the ATP14 gene." Arselin G., Vaillier J., Graves P.-V., Velours J. J. Biol. Chem. 271:20284-20290(1996) [PubMed: 8702761] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: D273-10B/A/H/U.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51673 Genomic DNA. Translation: AAC49436.1. U17243 Genomic DNA. Translation: AAB67340.1. AY558215 Genomic DNA. Translation: AAS56541.1.
PIR	S50379.
RefSeq	NP_013398.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3030N.
IntAct	Q12349. 125 interactions.
Protein family/group databases
TCDB	3.A.2.1.3. H+- or Na+-translocating F-type, V-type and A-type ATPase (F-ATPase) superfamily.
Proteomic databases
PeptideAtlas	Q12349.
PRIDE	Q12349.
Genome annotation databases
Ensembl	YLR295C. Saccharomyces cerevisiae. [Contig view]
GeneID	851002.
GenomeReviews	Gene locus YLR295C in contig Y13138_GR.
KEGG	sce:YLR295C.
NMPDR	fig|4932.3.peg.4415.
Organism-specific databases
CYGD	YLR295c.
SGD	S000004286. ATP14.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	Q12349.
OMA	Q12349. EEDWFVF.
Gene expression databases
ArrayExpress	Q12349.
GermOnline	YLR295C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019711. ATPase_F0-cplx_hsu. [Graphical view]
Pfam	PF10775. ATP_sub_h. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	967546.

Entry information

Entry name

ATP14_YEAST

Accession

Primary (citable) accession number: Q12349

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)