Q12338 (RNH2C_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease H2 subunit C
Short name=RNase H2 subunit C
Short name=Rnh2C

Alternative name(s):
RNase H(203)
Ribonuclease HI subunit C

Gene names

Name:	RNH203
Ordered Locus Names:	YLR154C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
Subunit structure	The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 556 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the RNase H2 subunit C family. Highly divergent.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication, removal of RNA primer Inferred by curator Ref.7. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD ribonuclease H2 complex Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 110	110	Ribonuclease H2 subunit C		PRO_0000248387
Experimental info
	Sequence conflict	64	1	F → L in AAS56535. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q12338 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 87A110F6A1754426	FASTA	110	12,482
	10 20 30 40 50 60 MTKDAVNLDA YTVSFMPFYT EYQGPTEEFK DYKFEDTIYF RGKELKREKS ATPSSSDNTT 70 80 90 100 110 SNTFSNGAIL SGNTITGKIV SVNNYEREGT DRNELARLQE LISLIDVINQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose." van den Berg M.A., de Steensma H.Y. Eur. J. Biochem. 231:704-713(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"RNase H2 of Saccharomyces cerevisiae is a complex of three proteins." Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J. Nucleic Acids Res. 32:407-414(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RNH201 AND RNH202.
[8]	Erratum Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J. Nucleic Acids Res. 32:1616-1616(2004)
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S79456 Genomic DNA. Translation: AAB35144.1. Z73326 Genomic DNA. Translation: CAA97726.1. U53879 Genomic DNA. Translation: AAB82388.1. AY558209 Genomic DNA. Translation: AAS56535.1. BK006945 Genomic DNA. Translation: DAA09465.1.
PIR	S65003.
RefSeq	NP_013255.1. NM_001182041.1.
3D structure databases
ProteinModelPortal	Q12338.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1839N.
IntAct	Q12338. 4 interactions.
MINT	MINT-399196.
Proteomic databases
PaxDb	Q12338.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR154C; YLR154C; YLR154C.
GeneID	850847.
KEGG	sce:YLR154C.
Organism-specific databases
CYGD	YLR154c.
SGD	S000004144. RNH203.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32288-MONOMER.
Gene expression databases
Genevestigator	Q12338.
GermOnline	YLR154C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	967141.

Entry information

Entry name

RNH2C_YEAST

Accession

Primary (citable) accession number: Q12338
Secondary accession number(s): D6VYE9, Q6Q5B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program