Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q12338 (RNH2C_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease H2 subunit C

Short name=RNase H2 subunit C
Short name=Rnh2C
Alternative name(s):
RNase H(203)
Ribonuclease HI subunit C
Gene names
Name:RNH203
Ordered Locus Names:YLR154C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length110 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Subunit structure

The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 556 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the RNase H2 subunit C family. Highly divergent.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RNH201P539422EBI-33805,EBI-15663
RNH202Q056352EBI-33805,EBI-33940

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 110110Ribonuclease H2 subunit C
PRO_0000248387

Experimental info

Sequence conflict641F → L in AAS56535. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q12338 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 87A110F6A1754426

FASTA11012,482
        10         20         30         40         50         60 
MTKDAVNLDA YTVSFMPFYT EYQGPTEEFK DYKFEDTIYF RGKELKREKS ATPSSSDNTT 

        70         80         90        100        110 
SNTFSNGAIL SGNTITGKIV SVNNYEREGT DRNELARLQE LISLIDVINQ 

« Hide

References

« Hide 'large scale' references
[1]"ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose."
van den Berg M.A., de Steensma H.Y.
Eur. J. Biochem. 231:704-713(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"RNase H2 of Saccharomyces cerevisiae is a complex of three proteins."
Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
Nucleic Acids Res. 32:407-414(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RNH201 AND RNH202.
[8]Erratum
Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
Nucleic Acids Res. 32:1616-1616(2004)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S79456 Genomic DNA. Translation: AAB35144.1.
Z73326 Genomic DNA. Translation: CAA97726.1.
U53879 Genomic DNA. Translation: AAB82388.1.
AY558209 Genomic DNA. Translation: AAS56535.1.
BK006945 Genomic DNA. Translation: DAA09465.1.
PIRS65003.
RefSeqNP_013255.1. NM_001182041.1.

3D structure databases

ProteinModelPortalQ12338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31423. 32 interactions.
DIPDIP-1839N.
IntActQ12338. 2 interactions.
MINTMINT-399196.

Proteomic databases

MaxQBQ12338.
PaxDbQ12338.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR154C; YLR154C; YLR154C.
GeneID850847.
KEGGsce:YLR154C.

Organism-specific databases

CYGDYLR154c.
SGDS000004144. RNH203.

Phylogenomic databases

OMAXALESAP.
OrthoDBEOG754J2P.

Enzyme and pathway databases

BioCycYEAST:G3O-32288-MONOMER.

Gene expression databases

GenevestigatorQ12338.

Family and domain databases

ProtoNetSearch...

Other

NextBio967141.

Entry information

Entry nameRNH2C_YEAST
AccessionPrimary (citable) accession number: Q12338
Secondary accession number(s): D6VYE9, Q6Q5B9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families