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Q12338

- RNH2C_YEAST

UniProt

Q12338 - RNH2C_YEAST

Protein

Ribonuclease H2 subunit C

Gene

RNH203

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. DNA replication, removal of RNA primer Source: SGD
    2. RNA catabolic process Source: SGD
    3. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32288-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease H2 subunit C
    Short name:
    RNase H2 subunit C
    Short name:
    Rnh2C
    Alternative name(s):
    RNase H(203)
    Ribonuclease HI subunit C
    Gene namesi
    Name:RNH203
    Ordered Locus Names:YLR154C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    CYGDiYLR154c.
    SGDiS000004144. RNH203.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. ribonuclease H2 complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 110110Ribonuclease H2 subunit CPRO_0000248387Add
    BLAST

    Proteomic databases

    MaxQBiQ12338.
    PaxDbiQ12338.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12338.

    Interactioni

    Subunit structurei

    The RNase 2 complex is a heterotrimer composed of the catalytic subunit RNH201 and of the non-catalytic subunits RNH202 and RNH203.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    RNH201P539422EBI-33805,EBI-15663
    RNH202Q056352EBI-33805,EBI-33940

    Protein-protein interaction databases

    BioGridi31423. 32 interactions.
    DIPiDIP-1839N.
    IntActiQ12338. 2 interactions.
    MINTiMINT-399196.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    OMAiXALESAP.
    OrthoDBiEOG754J2P.

    Sequencei

    Sequence statusi: Complete.

    Q12338-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKDAVNLDA YTVSFMPFYT EYQGPTEEFK DYKFEDTIYF RGKELKREKS    50
    ATPSSSDNTT SNTFSNGAIL SGNTITGKIV SVNNYEREGT DRNELARLQE 100
    LISLIDVINQ 110
    Length:110
    Mass (Da):12,482
    Last modified:November 1, 1996 - v1
    Checksum:i87A110F6A1754426
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641F → L in AAS56535. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79456 Genomic DNA. Translation: AAB35144.1.
    Z73326 Genomic DNA. Translation: CAA97726.1.
    U53879 Genomic DNA. Translation: AAB82388.1.
    AY558209 Genomic DNA. Translation: AAS56535.1.
    BK006945 Genomic DNA. Translation: DAA09465.1.
    PIRiS65003.
    RefSeqiNP_013255.1. NM_001182041.1.

    Genome annotation databases

    EnsemblFungiiYLR154C; YLR154C; YLR154C.
    GeneIDi850847.
    KEGGisce:YLR154C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S79456 Genomic DNA. Translation: AAB35144.1 .
    Z73326 Genomic DNA. Translation: CAA97726.1 .
    U53879 Genomic DNA. Translation: AAB82388.1 .
    AY558209 Genomic DNA. Translation: AAS56535.1 .
    BK006945 Genomic DNA. Translation: DAA09465.1 .
    PIRi S65003.
    RefSeqi NP_013255.1. NM_001182041.1.

    3D structure databases

    ProteinModelPortali Q12338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31423. 32 interactions.
    DIPi DIP-1839N.
    IntActi Q12338. 2 interactions.
    MINTi MINT-399196.

    Proteomic databases

    MaxQBi Q12338.
    PaxDbi Q12338.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR154C ; YLR154C ; YLR154C .
    GeneIDi 850847.
    KEGGi sce:YLR154C.

    Organism-specific databases

    CYGDi YLR154c.
    SGDi S000004144. RNH203.

    Phylogenomic databases

    OMAi XALESAP.
    OrthoDBi EOG754J2P.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32288-MONOMER.

    Miscellaneous databases

    NextBioi 967141.

    Gene expression databases

    Genevestigatori Q12338.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "ACS2, a Saccharomyces cerevisiae gene encoding acetyl-coenzyme A synthetase, essential for growth on glucose."
      van den Berg M.A., de Steensma H.Y.
      Eur. J. Biochem. 231:704-713(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "RNase H2 of Saccharomyces cerevisiae is a complex of three proteins."
      Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
      Nucleic Acids Res. 32:407-414(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH RNH201 AND RNH202.
    8. Erratum
      Jeong H.-S., Backlund P.S., Chen H.-C., Karavanov A.A., Crouch R.J.
      Nucleic Acids Res. 32:1616-1616(2004)

    Entry informationi

    Entry nameiRNH2C_YEAST
    AccessioniPrimary (citable) accession number: Q12338
    Secondary accession number(s): D6VYE9, Q6Q5B9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 556 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3