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Q12328

- TIM22_YEAST

UniProt

Q12328 - TIM22_YEAST

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Protein
Mitochondrial import inner membrane translocase subunit TIM22
Gene
TIM22, YDL217C, D0884
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins, such as mitochondrial carrier family members, into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps. Mediates the insertion of precursor proteins in a 3 step process. After the precursor is tethered to the translocase without losing energy from the Delta(psi), 2 energy-requiring steps are needed. First, Delta(psi) acts on the precursor protein and promotes its docking in the translocase complex. Then, Delta(psi) and an internal signal peptide together induce rapid gating transitions in one pore and closing of the other pore and drive membrane insertion to completion.3 Publications

GO - Molecular functioni

  1. mitochondrion targeting sequence binding Source: SGD
  2. protein binding Source: IntAct
  3. protein channel activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. protein import into mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29598-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM22
Gene namesi
Name:TIM22
Ordered Locus Names:YDL217C
ORF Names:D0884
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL217c.
SGDiS000002376. TIM22.

Subcellular locationi

Mitochondrion inner membrane; Multi-pass membrane protein
Note: Import into inner membrane protein requires TOM20 function.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei47 – 6721Helical; Reviewed prediction
Add
BLAST
Transmembranei152 – 17221Helical; Reviewed prediction
Add
BLAST
Transmembranei173 – 19321Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrial inner membrane Source: Reactome
  4. mitochondrial inner membrane protein insertion complex Source: SGD
  5. mitochondrial intermembrane space Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Mitochondrial import inner membrane translocase subunit TIM22
PRO_0000210301Add
BLAST

Proteomic databases

MaxQBiQ12328.
PaxDbiQ12328.

Expressioni

Gene expression databases

GenevestigatoriQ12328.

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM21P532202EBI-9132,EBI-23128

Protein-protein interaction databases

BioGridi31828. 60 interactions.
DIPiDIP-1142N.
IntActiQ12328. 7 interactions.
MINTiMINT-513851.
STRINGi4932.YDL217C.

Structurei

3D structure databases

ProteinModelPortaliQ12328.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim17/Tim22/Tim23 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5596.
GeneTreeiENSGT00390000016067.
HOGENOMiHOG000161169.
KOiK17790.
OMAiNFGYIGM.
OrthoDBiEOG7F7WN2.

Family and domain databases

InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12328-1 [UniParc]FASTAAdd to Basket

« Hide

MVYTGFGLEQ ISPAQKKPYN ELTPEEQGER GAEMIMNFMT SCPGKSVVSG    50
VTGFALGGVL GLFMASMAYD TPLHTPTPAN TAATATAGNI GVGGISRTVQ 100
QISDLPFRQQ MKLQFTDMGK KSYSSAKNFG YIGMIYAGVE CVIESLRAKN 150
DIYNGVTAGF FTGAGLAYKA GPQAALMGGA GFAAFSAAID LYMKSEDGRP 200
PQNDFKE 207
Length:207
Mass (Da):21,864
Last modified:November 1, 1997 - v1
Checksum:i3C94DBF31F413968
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99000 Genomic DNA. Translation: CAA67473.1.
Z74265 Genomic DNA. Translation: CAA98795.1.
AY558171 Genomic DNA. Translation: AAS56497.1.
BK006938 Genomic DNA. Translation: DAA11647.1.
PIRiS67776.
RefSeqiNP_010064.1. NM_001180277.1.

Genome annotation databases

EnsemblFungiiYDL217C; YDL217C; YDL217C.
GeneIDi851309.
KEGGisce:YDL217C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99000 Genomic DNA. Translation: CAA67473.1 .
Z74265 Genomic DNA. Translation: CAA98795.1 .
AY558171 Genomic DNA. Translation: AAS56497.1 .
BK006938 Genomic DNA. Translation: DAA11647.1 .
PIRi S67776.
RefSeqi NP_010064.1. NM_001180277.1.

3D structure databases

ProteinModelPortali Q12328.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31828. 60 interactions.
DIPi DIP-1142N.
IntActi Q12328. 7 interactions.
MINTi MINT-513851.
STRINGi 4932.YDL217C.

Protein family/group databases

TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBi Q12328.
PaxDbi Q12328.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL217C ; YDL217C ; YDL217C .
GeneIDi 851309.
KEGGi sce:YDL217C.

Organism-specific databases

CYGDi YDL217c.
SGDi S000002376. TIM22.

Phylogenomic databases

eggNOGi COG5596.
GeneTreei ENSGT00390000016067.
HOGENOMi HOG000161169.
KOi K17790.
OMAi NFGYIGM.
OrthoDBi EOG7F7WN2.

Enzyme and pathway databases

BioCyci YEAST:G3O-29598-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 968328.
PROi Q12328.

Gene expression databases

Genevestigatori Q12328.

Family and domain databases

InterProi IPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view ]
Pfami PF02466. Tim17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA."
    Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R.
    Yeast 13:163-169(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22."
    Sirrenberg C., Bauer M.D., Guiard B., Neupert W., Brunner M.
    Nature 384:582-585(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TIM10 AND TIM12.
  6. "Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5."
    Sirrenberg C., Endres M., Foelsch H., Stuart R.A., Neupert W., Brunner M.
    Nature 391:912-915(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIM10 AND TIM12.
  7. "Import of mitochondrial carriers mediated by essential proteins of the intermembrane space."
    Koehler C.M., Jarosch E., Tokatlidis K., Schmid K., Schweyen R.J., Schatz G.
    Science 279:369-373(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIM10 AND TIM12.
  8. "Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway."
    Kurz M., Martin H., Rassow J., Pfanner N., Ryan M.T.
    Mol. Biol. Cell 10:2461-2474(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane."
    Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E.
    Mol. Cell. Biol. 20:1187-1193(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A THE TIM22 COMPLEX WITH TIM12; TIM18 AND TIM54.
  10. "Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel."
    Kovermann P., Truscott K.N., Guiard B., Rehling P., Sepuri N.B., Mueller H., Jensen R.E., Wagner R., Pfanner N.
    Mol. Cell 9:363-373(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Protein insertion into the mitochondrial inner membrane by a twin-pore translocase."
    Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N.
    Science 299:1747-1751(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM12; TIM18 AND TIM54.

Entry informationi

Entry nameiTIM22_YEAST
AccessioniPrimary (citable) accession number: Q12328
Secondary accession number(s): D6VRD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi