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Protein

Mitochondrial import inner membrane translocase subunit TIM22

Gene

TIM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins, such as mitochondrial carrier family members, into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps. Mediates the insertion of precursor proteins in a 3 step process. After the precursor is tethered to the translocase without losing energy from the Delta(psi), 2 energy-requiring steps are needed. First, Delta(psi) acts on the precursor protein and promotes its docking in the translocase complex. Then, Delta(psi) and an internal signal peptide together induce rapid gating transitions in one pore and closing of the other pore and drive membrane insertion to completion.3 Publications

GO - Molecular functioni

  • mitochondrion targeting sequence binding Source: SGD
  • protein channel activity Source: SGD

GO - Biological processi

  • protein import into mitochondrial inner membrane Source: SGD
  • protein targeting to mitochondrion Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29598-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM22
Gene namesi
Name:TIM22
Ordered Locus Names:YDL217C
ORF Names:D0884
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL217C.
SGDiS000002376. TIM22.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei47 – 67HelicalSequence analysisAdd BLAST21
Transmembranei152 – 172HelicalSequence analysisAdd BLAST21
Transmembranei173 – 193HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103011 – 207Mitochondrial import inner membrane translocase subunit TIM22Add BLAST207

Proteomic databases

MaxQBiQ12328.
PRIDEiQ12328.

Interactioni

Subunit structurei

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIM21P532202EBI-9132,EBI-23128

Protein-protein interaction databases

BioGridi31828. 65 interactors.
DIPiDIP-1142N.
IntActiQ12328. 7 interactors.
MINTiMINT-513851.

Structurei

3D structure databases

ProteinModelPortaliQ12328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim17/Tim22/Tim23 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000016067.
HOGENOMiHOG000161169.
InParanoidiQ12328.
KOiK17790.
OMAiNFGYIGM.
OrthoDBiEOG092C5C6F.

Family and domain databases

InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYTGFGLEQ ISPAQKKPYN ELTPEEQGER GAEMIMNFMT SCPGKSVVSG
60 70 80 90 100
VTGFALGGVL GLFMASMAYD TPLHTPTPAN TAATATAGNI GVGGISRTVQ
110 120 130 140 150
QISDLPFRQQ MKLQFTDMGK KSYSSAKNFG YIGMIYAGVE CVIESLRAKN
160 170 180 190 200
DIYNGVTAGF FTGAGLAYKA GPQAALMGGA GFAAFSAAID LYMKSEDGRP

PQNDFKE
Length:207
Mass (Da):21,864
Last modified:November 1, 1997 - v1
Checksum:i3C94DBF31F413968
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99000 Genomic DNA. Translation: CAA67473.1.
Z74265 Genomic DNA. Translation: CAA98795.1.
AY558171 Genomic DNA. Translation: AAS56497.1.
BK006938 Genomic DNA. Translation: DAA11647.1.
PIRiS67776.
RefSeqiNP_010064.1. NM_001180277.1.

Genome annotation databases

EnsemblFungiiYDL217C; YDL217C; YDL217C.
GeneIDi851309.
KEGGisce:YDL217C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99000 Genomic DNA. Translation: CAA67473.1.
Z74265 Genomic DNA. Translation: CAA98795.1.
AY558171 Genomic DNA. Translation: AAS56497.1.
BK006938 Genomic DNA. Translation: DAA11647.1.
PIRiS67776.
RefSeqiNP_010064.1. NM_001180277.1.

3D structure databases

ProteinModelPortaliQ12328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31828. 65 interactors.
DIPiDIP-1142N.
IntActiQ12328. 7 interactors.
MINTiMINT-513851.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBiQ12328.
PRIDEiQ12328.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL217C; YDL217C; YDL217C.
GeneIDi851309.
KEGGisce:YDL217C.

Organism-specific databases

EuPathDBiFungiDB:YDL217C.
SGDiS000002376. TIM22.

Phylogenomic databases

GeneTreeiENSGT00390000016067.
HOGENOMiHOG000161169.
InParanoidiQ12328.
KOiK17790.
OMAiNFGYIGM.
OrthoDBiEOG092C5C6F.

Enzyme and pathway databases

BioCyciYEAST:G3O-29598-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiQ12328.

Family and domain databases

InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIM22_YEAST
AccessioniPrimary (citable) accession number: Q12328
Secondary accession number(s): D6VRD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.