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Q12328 (TIM22_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM22
Gene names
Name:TIM22
Ordered Locus Names:YDL217C
ORF Names:D0884
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length207 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins, such as mitochondrial carrier family members, into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps. Mediates the insertion of precursor proteins in a 3 step process. After the precursor is tethered to the translocase without losing energy from the Delta(psi), 2 energy-requiring steps are needed. First, Delta(psi) acts on the precursor protein and promotes its docking in the translocase complex. Then, Delta(psi) and an internal signal peptide together induce rapid gating transitions in one pore and closing of the other pore and drive membrane insertion to completion. Ref.5 Ref.10 Ref.11

Subunit structure

Component of the TIM22 complex, whose core is composed of TIM18, TIM22 and TIM54, associated with the peripheral proteins MRS5/TIM12 and the 70 kDa heterohexamer composed of TIM9 and TIM10 (or TIM8 and TIM13). Ref.9 Ref.11

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein. Note: Import into inner membrane protein requires TOM20 function. Ref.5 Ref.8

Sequence similarities

Belongs to the Tim17/Tim22/Tim23 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TIM21P532202EBI-9132,EBI-23128

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 207207Mitochondrial import inner membrane translocase subunit TIM22
PRO_0000210301

Regions

Transmembrane47 – 6721Helical; Potential
Transmembrane152 – 17221Helical; Potential
Transmembrane173 – 19321Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q12328 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 3C94DBF31F413968

FASTA20721,864
        10         20         30         40         50         60 
MVYTGFGLEQ ISPAQKKPYN ELTPEEQGER GAEMIMNFMT SCPGKSVVSG VTGFALGGVL 

        70         80         90        100        110        120 
GLFMASMAYD TPLHTPTPAN TAATATAGNI GVGGISRTVQ QISDLPFRQQ MKLQFTDMGK 

       130        140        150        160        170        180 
KSYSSAKNFG YIGMIYAGVE CVIESLRAKN DIYNGVTAGF FTGAGLAYKA GPQAALMGGA 

       190        200 
GFAAFSAAID LYMKSEDGRP PQNDFKE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one gene for Gly-tRNA."
Bahr A., Moeller-Rieker S., Hankeln T., Kraemer C., Protin U., Schmidt E.R.
Yeast 13:163-169(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22."
Sirrenberg C., Bauer M.D., Guiard B., Neupert W., Brunner M.
Nature 384:582-585(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TIM10 AND TIM12.
[6]"Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5."
Sirrenberg C., Endres M., Foelsch H., Stuart R.A., Neupert W., Brunner M.
Nature 391:912-915(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TIM10 AND TIM12.
[7]"Import of mitochondrial carriers mediated by essential proteins of the intermembrane space."
Koehler C.M., Jarosch E., Tokatlidis K., Schmid K., Schweyen R.J., Schatz G.
Science 279:369-373(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TIM10 AND TIM12.
[8]"Biogenesis of Tim proteins of the mitochondrial carrier import pathway: differential targeting mechanisms and crossing over with the main import pathway."
Kurz M., Martin H., Rassow J., Pfanner N., Ryan M.T.
Mol. Biol. Cell 10:2461-2474(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Tim18p, a new subunit of the TIM22 complex that mediates insertion of imported proteins into the yeast mitochondrial inner membrane."
Koehler C.M., Murphy M.P., Bally N.A., Leuenberger D., Oppliger W., Dolfini L., Junne T., Schatz G., Or E.
Mol. Cell. Biol. 20:1187-1193(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN A THE TIM22 COMPLEX WITH TIM12; TIM18 AND TIM54.
[10]"Tim22, the essential core of the mitochondrial protein insertion complex, forms a voltage-activated and signal-gated channel."
Kovermann P., Truscott K.N., Guiard B., Rehling P., Sepuri N.B., Mueller H., Jensen R.E., Wagner R., Pfanner N.
Mol. Cell 9:363-373(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Protein insertion into the mitochondrial inner membrane by a twin-pore translocase."
Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N.
Science 299:1747-1751(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, IDENTIFICATION IN THE TIM22 COMPLEX WITH TIM10; TIM12; TIM18 AND TIM54.
[12]Erratum
Rehling P., Model K., Brandner K., Kovermann P., Sickmann A., Meyer H.E., Kuehlbrandt W., Wagner R., Truscott K.N., Pfanner N.
Science 300:251-251(2003)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99000 Genomic DNA. Translation: CAA67473.1.
Z74265 Genomic DNA. Translation: CAA98795.1.
AY558171 Genomic DNA. Translation: AAS56497.1.
BK006938 Genomic DNA. Translation: DAA11647.1.
PIRS67776.
RefSeqNP_010064.1. NM_001180277.1.

3D structure databases

ProteinModelPortalQ12328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31828. 60 interactions.
DIPDIP-1142N.
IntActQ12328. 7 interactions.
MINTMINT-513851.
STRING4932.YDL217C.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

PaxDbQ12328.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL217C; YDL217C; YDL217C.
GeneID851309.
KEGGsce:YDL217C.

Organism-specific databases

CYGDYDL217c.
SGDS000002376. TIM22.

Phylogenomic databases

eggNOGCOG5596.
GeneTreeENSGT00390000016067.
HOGENOMHOG000161169.
KOK17790.
OMANFGYIGM.
OrthoDBEOG7F7WN2.

Enzyme and pathway databases

BioCycYEAST:G3O-29598-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).
REACT_85873. Metabolism of proteins.

Gene expression databases

GenevestigatorQ12328.

Family and domain databases

InterProIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamPF02466. Tim17. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968328.
PROQ12328.

Entry information

Entry nameTIM22_YEAST
AccessionPrimary (citable) accession number: Q12328
Secondary accession number(s): D6VRD7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families