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Protein

Hydroxyacylglutathione hydrolase, mitochondrial

Gene

GLO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLO1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (GLO4), Hydroxyacylglutathione hydrolase, cytoplasmic isozyme (GLO2)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1; via tele nitrogenBy similarity
Metal bindingi71 – 711Zinc 1; via pros nitrogenBy similarity
Metal bindingi73 – 731Zinc 2By similarity
Metal bindingi74 – 741Zinc 2; via tele nitrogenBy similarity
Metal bindingi131 – 1311Zinc 1; via tele nitrogenBy similarity
Metal bindingi154 – 1541Zinc 1By similarity
Metal bindingi154 – 1541Zinc 2By similarity
Metal bindingi198 – 1981Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YOR040W-MONOMER.
BRENDAi3.1.2.6. 984.
ReactomeiR-SCE-70268. Pyruvate metabolism.
SABIO-RKQ12320.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:GLO4
Synonyms:GER1
Ordered Locus Names:YOR040W
ORF Names:O2758, OR26.33
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR040W.
SGDiS000005566. GLO4.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 285Hydroxyacylglutathione hydrolase, mitochondrialPRO_0000012285
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiQ12320.

Interactioni

Protein-protein interaction databases

BioGridi34442. 49 interactions.
DIPiDIP-4183N.
IntActiQ12320. 1 interaction.
MINTiMINT-528877.

Structurei

3D structure databases

ProteinModelPortaliQ12320.
SMRiQ12320. Positions 26-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
InParanoidiQ12320.
KOiK01069.
OMAiMIVERIW.
OrthoDBiEOG7RRFJR.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR032282. HAGH_C.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLQQIRN MHVKPIKMRW LTGGVNYSYL LSTEDRRNSW LIDPAEPLEV
60 70 80 90 100
SPKLSAEEKK SIDAIVNTHH HYDHSGGNLA LYSILCQENS GHDIKIIGGS
110 120 130 140 150
KSSPGVTEVP DNLQQYHLGN LRVTCIRTPC HTKDSICYYI KDLETGEQCI
160 170 180 190 200
FTGDTLFIAG CGRFFEGTGR DMDMALNQIM LRAVGETNWN KVKIYPGHEY
210 220 230 240 250
TKGNVSFIRA KIYSDIGQNK EFDALEQYCK SNECTTGHFT LRDELGYNPF
260 270 280
MRLDDRAVRL AVGDTAGTYP RSVVMQELRK LKNAM
Length:285
Mass (Da):32,339
Last modified:November 1, 1996 - v1
Checksum:i09DF2E4B5B9C0E52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82893 Genomic DNA. Translation: CAA58065.1.
X87331 Genomic DNA. Translation: CAA60759.1.
Z74948 Genomic DNA. Translation: CAA99230.1.
AY692965 Genomic DNA. Translation: AAT92984.1.
BK006948 Genomic DNA. Translation: DAA10823.1.
PIRiS62179.
RefSeqiNP_014683.1. NM_001183459.1.

Genome annotation databases

EnsemblFungiiYOR040W; YOR040W; YOR040W.
GeneIDi854205.
KEGGisce:YOR040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82893 Genomic DNA. Translation: CAA58065.1.
X87331 Genomic DNA. Translation: CAA60759.1.
Z74948 Genomic DNA. Translation: CAA99230.1.
AY692965 Genomic DNA. Translation: AAT92984.1.
BK006948 Genomic DNA. Translation: DAA10823.1.
PIRiS62179.
RefSeqiNP_014683.1. NM_001183459.1.

3D structure databases

ProteinModelPortaliQ12320.
SMRiQ12320. Positions 26-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34442. 49 interactions.
DIPiDIP-4183N.
IntActiQ12320. 1 interaction.
MINTiMINT-528877.

Proteomic databases

MaxQBiQ12320.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR040W; YOR040W; YOR040W.
GeneIDi854205.
KEGGisce:YOR040W.

Organism-specific databases

EuPathDBiFungiDB:YOR040W.
SGDiS000005566. GLO4.

Phylogenomic databases

GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
InParanoidiQ12320.
KOiK01069.
OMAiMIVERIW.
OrthoDBiEOG7RRFJR.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BioCyciYEAST:YOR040W-MONOMER.
BRENDAi3.1.2.6. 984.
ReactomeiR-SCE-70268. Pyruvate metabolism.
SABIO-RKQ12320.

Miscellaneous databases

NextBioi976050.
PROiQ12320.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR032282. HAGH_C.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and phenotypic analysis of two glyoxalase II encoding genes from Saccharomyces cerevisiae, GLO2 and GLO4, and intracellular localization of the corresponding proteins."
    Bito A., Haider M., Hadler I., Breitenbach M.
    J. Biol. Chem. 272:21509-21519(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AP3.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiGLO4_YEAST
AccessioniPrimary (citable) accession number: Q12320
Secondary accession number(s): D6W2A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.