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Protein

Hydroxyacylglutathione hydrolase, mitochondrial

Gene

GLO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLO1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (GLO4), Hydroxyacylglutathione hydrolase, cytoplasmic isozyme (GLO2)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1; via tele nitrogenBy similarity1
Metal bindingi71Zinc 1; via pros nitrogenBy similarity1
Metal bindingi73Zinc 2By similarity1
Metal bindingi74Zinc 2; via tele nitrogenBy similarity1
Metal bindingi131Zinc 1; via tele nitrogenBy similarity1
Metal bindingi154Zinc 1By similarity1
Metal bindingi154Zinc 2By similarity1
Metal bindingi198Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YOR040W-MONOMER
BRENDAi3.1.2.6 984
ReactomeiR-SCE-70268 Pyruvate metabolism
SABIO-RKiQ12320
UniPathwayiUPA00619; UER00676

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:GLO4
Synonyms:GER1
Ordered Locus Names:YOR040W
ORF Names:O2758, OR26.33
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR040W
SGDiS000005566 GLO4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000012285? – 285Hydroxyacylglutathione hydrolase, mitochondrial
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiQ12320
PaxDbiQ12320
PRIDEiQ12320

Interactioni

Protein-protein interaction databases

BioGridi34442, 113 interactors
DIPiDIP-4183N
IntActiQ12320, 1 interactor
MINTiQ12320
STRINGi4932.YOR040W

Structurei

3D structure databases

ProteinModelPortaliQ12320
SMRiQ12320
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00530000063033
HOGENOMiHOG000058041
InParanoidiQ12320
KOiK01069
OrthoDBiEOG092C4YMK

Family and domain databases

CDDicd07723 hydroxyacylglutathione_hydrola, 1 hit
Gene3Di3.60.15.10, 1 hit
InterProiView protein in InterPro
IPR035680 Clx_II_MBL
IPR032282 HAGH_C
IPR001279 Metallo-B-lactamas
IPR036866 RibonucZ/Hydroxyglut_hydro
PfamiView protein in Pfam
PF16123 HAGH_C, 1 hit
PF00753 Lactamase_B, 1 hit
SMARTiView protein in SMART
SM00849 Lactamase_B, 1 hit
SUPFAMiSSF56281 SSF56281, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12320-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLLQQIRN MHVKPIKMRW LTGGVNYSYL LSTEDRRNSW LIDPAEPLEV
60 70 80 90 100
SPKLSAEEKK SIDAIVNTHH HYDHSGGNLA LYSILCQENS GHDIKIIGGS
110 120 130 140 150
KSSPGVTEVP DNLQQYHLGN LRVTCIRTPC HTKDSICYYI KDLETGEQCI
160 170 180 190 200
FTGDTLFIAG CGRFFEGTGR DMDMALNQIM LRAVGETNWN KVKIYPGHEY
210 220 230 240 250
TKGNVSFIRA KIYSDIGQNK EFDALEQYCK SNECTTGHFT LRDELGYNPF
260 270 280
MRLDDRAVRL AVGDTAGTYP RSVVMQELRK LKNAM
Length:285
Mass (Da):32,339
Last modified:November 1, 1996 - v1
Checksum:i09DF2E4B5B9C0E52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82893 Genomic DNA Translation: CAA58065.1
X87331 Genomic DNA Translation: CAA60759.1
Z74948 Genomic DNA Translation: CAA99230.1
AY692965 Genomic DNA Translation: AAT92984.1
BK006948 Genomic DNA Translation: DAA10823.1
PIRiS62179
RefSeqiNP_014683.1, NM_001183459.1

Genome annotation databases

EnsemblFungiiYOR040W; YOR040W; YOR040W
GeneIDi854205
KEGGisce:YOR040W

Similar proteinsi

Entry informationi

Entry nameiGLO4_YEAST
AccessioniPrimary (citable) accession number: Q12320
Secondary accession number(s): D6W2A7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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