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Q12308

- TFC8_YEAST

UniProt

Q12308 - TFC8_YEAST

Protein

Transcription factor tau 60 kDa subunit

Gene

TFC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei358 – 3581Involved in the interaction with TFC6

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. 5S class rRNA transcription from RNA polymerase III type 1 promoter Source: SGD
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. RNA polymerase III type 1 promoter transcriptional preinitiation complex assembly Source: GOC
    4. RNA polymerase III type 2 promoter transcriptional preinitiation complex assembly Source: GOC
    5. transcription from a RNA polymerase III hybrid type promoter Source: SGD
    6. transcription from RNA polymerase III promoter Source: SGD
    7. transcription from RNA polymerase III type 2 promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33926-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor tau 60 kDa subunit
    Alternative name(s):
    TFIIIC 60 kDa subunit
    Transcription factor C subunit 8
    Gene namesi
    Name:TFC8
    Ordered Locus Names:YPL007C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL007c.
    SGDiS000005928. TFC8.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. transcription factor TFIIIC complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 588588Transcription factor tau 60 kDa subunitPRO_0000252484Add
    BLAST

    Proteomic databases

    MaxQBiQ12308.
    PaxDbiQ12308.
    PeptideAtlasiQ12308.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12308.

    Interactioni

    Subunit structurei

    Heterodimer with TFC6. Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with SPT15 and directly with TFC6.3 Publications

    Protein-protein interaction databases

    BioGridi36170. 15 interactions.
    DIPiDIP-6743N.
    IntActiQ12308. 7 interactions.
    MINTiMINT-642941.
    STRINGi4932.YPL007C.

    Structurei

    Secondary structure

    1
    588
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 93
    Beta strandi15 – 184
    Beta strandi21 – 233
    Beta strandi29 – 324
    Beta strandi34 – 429
    Beta strandi49 – 513
    Helixi52 – 543
    Beta strandi56 – 616
    Turni68 – 714
    Beta strandi88 – 936
    Beta strandi95 – 984
    Beta strandi100 – 1045
    Beta strandi109 – 1135
    Beta strandi116 – 1205
    Turni128 – 1303
    Beta strandi132 – 1376
    Beta strandi139 – 1424
    Beta strandi144 – 1485
    Beta strandi151 – 1577
    Turni162 – 1643
    Beta strandi169 – 1757
    Beta strandi185 – 1917
    Beta strandi194 – 1996
    Beta strandi204 – 2074
    Beta strandi211 – 2144
    Beta strandi218 – 2225
    Beta strandi232 – 2354
    Beta strandi238 – 2425
    Beta strandi244 – 2518
    Turni252 – 2554
    Beta strandi256 – 2616
    Beta strandi270 – 2734
    Beta strandi280 – 2845
    Beta strandi289 – 30113
    Helixi307 – 31610
    Turni317 – 3226
    Beta strandi323 – 3275
    Beta strandi330 – 3389
    Beta strandi342 – 35211
    Beta strandi361 – 3633
    Beta strandi366 – 37510
    Helixi386 – 3949
    Turni395 – 3973
    Helixi412 – 4154
    Helixi423 – 43210
    Helixi434 – 4429
    Turni443 – 4453
    Beta strandi446 – 4483
    Helixi452 – 46413
    Helixi465 – 4684
    Helixi472 – 48413
    Beta strandi495 – 5006
    Beta strandi503 – 5097
    Helixi510 – 5123
    Beta strandi516 – 5205
    Beta strandi526 – 5294
    Beta strandi531 – 5333
    Beta strandi542 – 5443
    Turni546 – 5483
    Beta strandi551 – 5533
    Helixi555 – 5573
    Beta strandi559 – 5613
    Helixi565 – 5739
    Beta strandi575 – 5773
    Turni579 – 5813

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2J04X-ray3.20A/C1-588[»]
    ProteinModelPortaliQ12308.
    SMRiQ12308. Positions 2-588.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12308.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni399 – 588190Sufficient for SPT15-bindingAdd
    BLAST

    Phylogenomic databases

    eggNOGiNOG39185.
    HOGENOMiHOG000074727.
    KOiK15207.
    OMAiKICPVSK.
    OrthoDBiEOG71GB4Q.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR024764. TFIIIC_Znf.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF12660. zf-TFIIIC. 1 hit.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q12308-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLKDLLVD RKEFEDWKNN LTWARDGTLY LTTFPDISIG QPKYAKDINC    50
    NSKNLFHVKE FPLEFENKLD FELAQQNGLL NSQPVCYPRV CKPSPIDDWM 100
    AVLSNNGNVS VFKDNKMLTN LDSKGNLSSR TYHCFEWNPI ESSIVVGNED 150
    GELQFFSIRK NSENTPEFYF ESSIRLSDAG SKDWVTHIVW YEDVLVAALS 200
    NNSVFSMTVS ASSHQPVSRM IQNASRRKIT DLKIVDYKVV LTCPGYVHKI 250
    DLKNYSISSL KTGSLENFHI IPLNHEKEST ILLMSNKTSY KVLLEDELHV 300
    TADNIIAPYL EKKFKKWSTI WNEFNNYETT LVIHGISLSP DGYSIAIVYD 350
    MERVAFKYKI ASEQSFNIMF APLYHTWTIS ERAVGLAWYQ TYQIYNQSLP 400
    KLPENFSMNK KLLNGNYPIS LDFQSYLNAL MKSEEMRIIM FLNMTIDKPS 450
    ILSFLEALYE YAINKKSELT NSFDLACVLS IAAILKREAP IYNGTLLMKN 500
    SFLEETFNLE SFTADPETVT STTNNTWKRC GVTLLPILTT HVKICPVSKQ 550
    RVIDIKRDDL NDYGWFTRGL LERFNEISVY CGTTLEVM 588
    Length:588
    Mass (Da):67,683
    Last modified:November 1, 1996 - v1
    Checksum:iBDBD7BBE947BAC63
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti36 – 361D → T AA sequence (PubMed:10567530)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71255 Genomic DNA. Translation: CAA95034.1.
    Z48483 Genomic DNA. Translation: CAA88379.1.
    U33335 Genomic DNA. Translation: AAB68098.1.
    BK006949 Genomic DNA. Translation: DAA11421.1.
    PIRiS52524.
    RefSeqiNP_015318.1. NM_001183821.1.

    Genome annotation databases

    EnsemblFungiiYPL007C; YPL007C; YPL007C.
    GeneIDi856100.
    KEGGisce:YPL007C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71255 Genomic DNA. Translation: CAA95034.1 .
    Z48483 Genomic DNA. Translation: CAA88379.1 .
    U33335 Genomic DNA. Translation: AAB68098.1 .
    BK006949 Genomic DNA. Translation: DAA11421.1 .
    PIRi S52524.
    RefSeqi NP_015318.1. NM_001183821.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2J04 X-ray 3.20 A/C 1-588 [» ]
    ProteinModelPortali Q12308.
    SMRi Q12308. Positions 2-588.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36170. 15 interactions.
    DIPi DIP-6743N.
    IntActi Q12308. 7 interactions.
    MINTi MINT-642941.
    STRINGi 4932.YPL007C.

    Proteomic databases

    MaxQBi Q12308.
    PaxDbi Q12308.
    PeptideAtlasi Q12308.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL007C ; YPL007C ; YPL007C .
    GeneIDi 856100.
    KEGGi sce:YPL007C.

    Organism-specific databases

    CYGDi YPL007c.
    SGDi S000005928. TFC8.

    Phylogenomic databases

    eggNOGi NOG39185.
    HOGENOMi HOG000074727.
    KOi K15207.
    OMAi KICPVSK.
    OrthoDBi EOG71GB4Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33926-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12308.
    NextBioi 981146.

    Gene expression databases

    Genevestigatori Q12308.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR024764. TFIIIC_Znf.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF12660. zf-TFIIIC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Expression, proteolytic analysis, reconstitution, and crystallization of the tau60/tau91 subcomplex of yeast TFIIIC."
      Mylona A., Acker J., Fernandez-Tornero C., Sentenac A., Mueller C.W.
      Protein Expr. Purif. 45:255-261(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CRYSTALLIZATION, INTERACTION WITH TFC6.
    4. "A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein."
      Deprez E., Arrebola R., Conesa C., Sentenac A.
      Mol. Cell. Biol. 19:8042-8051(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-36, FUNCTION, IDENTIFICATION IN THE TFIIIC COMPLEX, INTERACTION WITH SPT15.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly."
      Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W.
      Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) IN COMPLEX WITH TFC6, INTERACTION WITH SPT15, SITE.

    Entry informationi

    Entry nameiTFC8_YEAST
    AccessioniPrimary (citable) accession number: Q12308
    Secondary accession number(s): D6W405
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2070 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3