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Q12308 (TFC8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor tau 60 kDa subunit
Alternative name(s):
TFIIIC 60 kDa subunit
Transcription factor C subunit 8
Gene names
Name:TFC8
Ordered Locus Names:YPL007C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length588 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability. Ref.4

Subunit structure

Heterodimer with TFC6. Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with SPT15 and directly with TFC6. Ref.3 Ref.4 Ref.8

Subcellular location

Nucleus Ref.5.

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 588588Transcription factor tau 60 kDa subunit
PRO_0000252484

Regions

Region399 – 588190Sufficient for SPT15-binding

Sites

Site3581Involved in the interaction with TFC6

Experimental info

Sequence conflict361D → T AA sequence Ref.4

Secondary structure

......................................................................................................................... 588
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q12308 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BDBD7BBE947BAC63

FASTA58867,683
        10         20         30         40         50         60 
MKLLKDLLVD RKEFEDWKNN LTWARDGTLY LTTFPDISIG QPKYAKDINC NSKNLFHVKE 

        70         80         90        100        110        120 
FPLEFENKLD FELAQQNGLL NSQPVCYPRV CKPSPIDDWM AVLSNNGNVS VFKDNKMLTN 

       130        140        150        160        170        180 
LDSKGNLSSR TYHCFEWNPI ESSIVVGNED GELQFFSIRK NSENTPEFYF ESSIRLSDAG 

       190        200        210        220        230        240 
SKDWVTHIVW YEDVLVAALS NNSVFSMTVS ASSHQPVSRM IQNASRRKIT DLKIVDYKVV 

       250        260        270        280        290        300 
LTCPGYVHKI DLKNYSISSL KTGSLENFHI IPLNHEKEST ILLMSNKTSY KVLLEDELHV 

       310        320        330        340        350        360 
TADNIIAPYL EKKFKKWSTI WNEFNNYETT LVIHGISLSP DGYSIAIVYD MERVAFKYKI 

       370        380        390        400        410        420 
ASEQSFNIMF APLYHTWTIS ERAVGLAWYQ TYQIYNQSLP KLPENFSMNK KLLNGNYPIS 

       430        440        450        460        470        480 
LDFQSYLNAL MKSEEMRIIM FLNMTIDKPS ILSFLEALYE YAINKKSELT NSFDLACVLS 

       490        500        510        520        530        540 
IAAILKREAP IYNGTLLMKN SFLEETFNLE SFTADPETVT STTNNTWKRC GVTLLPILTT 

       550        560        570        580 
HVKICPVSKQ RVIDIKRDDL NDYGWFTRGL LERFNEISVY CGTTLEVM 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Expression, proteolytic analysis, reconstitution, and crystallization of the tau60/tau91 subcomplex of yeast TFIIIC."
Mylona A., Acker J., Fernandez-Tornero C., Sentenac A., Mueller C.W.
Protein Expr. Purif. 45:255-261(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CRYSTALLIZATION, INTERACTION WITH TFC6.
[4]"A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein."
Deprez E., Arrebola R., Conesa C., Sentenac A.
Mol. Cell. Biol. 19:8042-8051(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-36, FUNCTION, IDENTIFICATION IN THE TFIIIC COMPLEX, INTERACTION WITH SPT15.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly."
Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W.
Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) IN COMPLEX WITH TFC6, INTERACTION WITH SPT15, SITE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z71255 Genomic DNA. Translation: CAA95034.1.
Z48483 Genomic DNA. Translation: CAA88379.1.
U33335 Genomic DNA. Translation: AAB68098.1.
BK006949 Genomic DNA. Translation: DAA11421.1.
PIRS52524.
RefSeqNP_015318.1. NM_001183821.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2J04X-ray3.20A/C1-588[»]
ProteinModelPortalQ12308.
SMRQ12308. Positions 2-588.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36170. 15 interactions.
DIPDIP-6743N.
IntActQ12308. 7 interactions.
MINTMINT-642941.
STRING4932.YPL007C.

Proteomic databases

PaxDbQ12308.
PeptideAtlasQ12308.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL007C; YPL007C; YPL007C.
GeneID856100.
KEGGsce:YPL007C.

Organism-specific databases

CYGDYPL007c.
SGDS000005928. TFC8.

Phylogenomic databases

eggNOGNOG39185.
HOGENOMHOG000074727.
KOK15207.
OMAKICPVSK.
OrthoDBEOG71GB4Q.

Enzyme and pathway databases

BioCycYEAST:G3O-33926-MONOMER.

Gene expression databases

GenevestigatorQ12308.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
InterProIPR024764. TFIIIC_Znf.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamPF12660. zf-TFIIIC. 1 hit.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceQ12308.
NextBio981146.

Entry information

Entry nameTFC8_YEAST
AccessionPrimary (citable) accession number: Q12308
Secondary accession number(s): D6W405
Entry history
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references