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Q12308

- TFC8_YEAST

UniProt

Q12308 - TFC8_YEAST

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Protein
Transcription factor tau 60 kDa subunit
Gene
TFC8, YPL007C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei358 – 3581Involved in the interaction with TFC6

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. 5S class rRNA transcription from RNA polymerase III type 1 promoter Source: SGD
  2. RNA polymerase III type 1 promoter transcriptional preinitiation complex assembly Source: GOC
  3. RNA polymerase III type 2 promoter transcriptional preinitiation complex assembly Source: GOC
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription from RNA polymerase III promoter Source: SGD
  6. transcription from RNA polymerase III type 2 promoter Source: SGD
  7. transcription from a RNA polymerase III hybrid type promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33926-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor tau 60 kDa subunit
Alternative name(s):
TFIIIC 60 kDa subunit
Transcription factor C subunit 8
Gene namesi
Name:TFC8
Ordered Locus Names:YPL007C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL007c.
SGDiS000005928. TFC8.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. transcription factor TFIIIC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 588588Transcription factor tau 60 kDa subunit
PRO_0000252484Add
BLAST

Proteomic databases

MaxQBiQ12308.
PaxDbiQ12308.
PeptideAtlasiQ12308.

Expressioni

Gene expression databases

GenevestigatoriQ12308.

Interactioni

Subunit structurei

Heterodimer with TFC6. Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with SPT15 and directly with TFC6.3 Publications

Protein-protein interaction databases

BioGridi36170. 15 interactions.
DIPiDIP-6743N.
IntActiQ12308. 7 interactions.
MINTiMINT-642941.
STRINGi4932.YPL007C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93
Beta strandi15 – 184
Beta strandi21 – 233
Beta strandi29 – 324
Beta strandi34 – 429
Beta strandi49 – 513
Helixi52 – 543
Beta strandi56 – 616
Turni68 – 714
Beta strandi88 – 936
Beta strandi95 – 984
Beta strandi100 – 1045
Beta strandi109 – 1135
Beta strandi116 – 1205
Turni128 – 1303
Beta strandi132 – 1376
Beta strandi139 – 1424
Beta strandi144 – 1485
Beta strandi151 – 1577
Turni162 – 1643
Beta strandi169 – 1757
Beta strandi185 – 1917
Beta strandi194 – 1996
Beta strandi204 – 2074
Beta strandi211 – 2144
Beta strandi218 – 2225
Beta strandi232 – 2354
Beta strandi238 – 2425
Beta strandi244 – 2518
Turni252 – 2554
Beta strandi256 – 2616
Beta strandi270 – 2734
Beta strandi280 – 2845
Beta strandi289 – 30113
Helixi307 – 31610
Turni317 – 3226
Beta strandi323 – 3275
Beta strandi330 – 3389
Beta strandi342 – 35211
Beta strandi361 – 3633
Beta strandi366 – 37510
Helixi386 – 3949
Turni395 – 3973
Helixi412 – 4154
Helixi423 – 43210
Helixi434 – 4429
Turni443 – 4453
Beta strandi446 – 4483
Helixi452 – 46413
Helixi465 – 4684
Helixi472 – 48413
Beta strandi495 – 5006
Beta strandi503 – 5097
Helixi510 – 5123
Beta strandi516 – 5205
Beta strandi526 – 5294
Beta strandi531 – 5333
Beta strandi542 – 5443
Turni546 – 5483
Beta strandi551 – 5533
Helixi555 – 5573
Beta strandi559 – 5613
Helixi565 – 5739
Beta strandi575 – 5773
Turni579 – 5813

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J04X-ray3.20A/C1-588[»]
ProteinModelPortaliQ12308.
SMRiQ12308. Positions 2-588.

Miscellaneous databases

EvolutionaryTraceiQ12308.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni399 – 588190Sufficient for SPT15-binding
Add
BLAST

Phylogenomic databases

eggNOGiNOG39185.
HOGENOMiHOG000074727.
KOiK15207.
OMAiKICPVSK.
OrthoDBiEOG71GB4Q.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024764. TFIIIC_Znf.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12660. zf-TFIIIC. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequencei

Sequence statusi: Complete.

Q12308-1 [UniParc]FASTAAdd to Basket

« Hide

MKLLKDLLVD RKEFEDWKNN LTWARDGTLY LTTFPDISIG QPKYAKDINC    50
NSKNLFHVKE FPLEFENKLD FELAQQNGLL NSQPVCYPRV CKPSPIDDWM 100
AVLSNNGNVS VFKDNKMLTN LDSKGNLSSR TYHCFEWNPI ESSIVVGNED 150
GELQFFSIRK NSENTPEFYF ESSIRLSDAG SKDWVTHIVW YEDVLVAALS 200
NNSVFSMTVS ASSHQPVSRM IQNASRRKIT DLKIVDYKVV LTCPGYVHKI 250
DLKNYSISSL KTGSLENFHI IPLNHEKEST ILLMSNKTSY KVLLEDELHV 300
TADNIIAPYL EKKFKKWSTI WNEFNNYETT LVIHGISLSP DGYSIAIVYD 350
MERVAFKYKI ASEQSFNIMF APLYHTWTIS ERAVGLAWYQ TYQIYNQSLP 400
KLPENFSMNK KLLNGNYPIS LDFQSYLNAL MKSEEMRIIM FLNMTIDKPS 450
ILSFLEALYE YAINKKSELT NSFDLACVLS IAAILKREAP IYNGTLLMKN 500
SFLEETFNLE SFTADPETVT STTNNTWKRC GVTLLPILTT HVKICPVSKQ 550
RVIDIKRDDL NDYGWFTRGL LERFNEISVY CGTTLEVM 588
Length:588
Mass (Da):67,683
Last modified:November 1, 1996 - v1
Checksum:iBDBD7BBE947BAC63
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361D → T AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71255 Genomic DNA. Translation: CAA95034.1.
Z48483 Genomic DNA. Translation: CAA88379.1.
U33335 Genomic DNA. Translation: AAB68098.1.
BK006949 Genomic DNA. Translation: DAA11421.1.
PIRiS52524.
RefSeqiNP_015318.1. NM_001183821.1.

Genome annotation databases

EnsemblFungiiYPL007C; YPL007C; YPL007C.
GeneIDi856100.
KEGGisce:YPL007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71255 Genomic DNA. Translation: CAA95034.1 .
Z48483 Genomic DNA. Translation: CAA88379.1 .
U33335 Genomic DNA. Translation: AAB68098.1 .
BK006949 Genomic DNA. Translation: DAA11421.1 .
PIRi S52524.
RefSeqi NP_015318.1. NM_001183821.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2J04 X-ray 3.20 A/C 1-588 [» ]
ProteinModelPortali Q12308.
SMRi Q12308. Positions 2-588.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36170. 15 interactions.
DIPi DIP-6743N.
IntActi Q12308. 7 interactions.
MINTi MINT-642941.
STRINGi 4932.YPL007C.

Proteomic databases

MaxQBi Q12308.
PaxDbi Q12308.
PeptideAtlasi Q12308.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL007C ; YPL007C ; YPL007C .
GeneIDi 856100.
KEGGi sce:YPL007C.

Organism-specific databases

CYGDi YPL007c.
SGDi S000005928. TFC8.

Phylogenomic databases

eggNOGi NOG39185.
HOGENOMi HOG000074727.
KOi K15207.
OMAi KICPVSK.
OrthoDBi EOG71GB4Q.

Enzyme and pathway databases

BioCyci YEAST:G3O-33926-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12308.
NextBioi 981146.

Gene expression databases

Genevestigatori Q12308.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR024764. TFIIIC_Znf.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF12660. zf-TFIIIC. 1 hit.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Expression, proteolytic analysis, reconstitution, and crystallization of the tau60/tau91 subcomplex of yeast TFIIIC."
    Mylona A., Acker J., Fernandez-Tornero C., Sentenac A., Mueller C.W.
    Protein Expr. Purif. 45:255-261(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF N-TERMINUS, CRYSTALLIZATION, INTERACTION WITH TFC6.
  4. "A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein."
    Deprez E., Arrebola R., Conesa C., Sentenac A.
    Mol. Cell. Biol. 19:8042-8051(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-36, FUNCTION, IDENTIFICATION IN THE TFIIIC COMPLEX, INTERACTION WITH SPT15.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly."
    Mylona A., Fernandez-Tornero C., Legrand P., Haupt M., Sentenac A., Acker J., Mueller C.W.
    Mol. Cell 24:221-232(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) IN COMPLEX WITH TFC6, INTERACTION WITH SPT15, SITE.

Entry informationi

Entry nameiTFC8_YEAST
AccessioniPrimary (citable) accession number: Q12308
Secondary accession number(s): D6W405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

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