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Protein

Transcription factor tau 60 kDa subunit

Gene

TFC8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei358Involved in the interaction with TFC61

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33926-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor tau 60 kDa subunit
Alternative name(s):
TFIIIC 60 kDa subunit
Transcription factor C subunit 8
Gene namesi
Name:TFC8
Ordered Locus Names:YPL007C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL007C.
SGDiS000005928. TFC8.

Subcellular locationi

GO - Cellular componenti

  • transcription factor TFIIIC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002524841 – 588Transcription factor tau 60 kDa subunitAdd BLAST588

Proteomic databases

MaxQBiQ12308.
PRIDEiQ12308.
TopDownProteomicsiQ12308.

Interactioni

Subunit structurei

Heterodimer with TFC6. Component of the TFIIIC complex composed of TFC1, TFC3, TFC4, TFC6, TFC7 and TFC8. The subunits are organized in two globular domains, tauA and tauB, connected by a proteolysis-sensitive and flexible linker. Interacts with SPT15 and directly with TFC6.3 Publications

Protein-protein interaction databases

BioGridi36170. 15 interactors.
DIPiDIP-6743N.
IntActiQ12308. 7 interactors.
MINTiMINT-642941.

Structurei

Secondary structure

1588
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Beta strandi15 – 18Combined sources4
Beta strandi21 – 23Combined sources3
Beta strandi29 – 32Combined sources4
Beta strandi34 – 42Combined sources9
Beta strandi49 – 51Combined sources3
Helixi52 – 54Combined sources3
Beta strandi56 – 61Combined sources6
Turni68 – 71Combined sources4
Beta strandi88 – 93Combined sources6
Beta strandi95 – 98Combined sources4
Beta strandi100 – 104Combined sources5
Beta strandi109 – 113Combined sources5
Beta strandi116 – 120Combined sources5
Turni128 – 130Combined sources3
Beta strandi132 – 137Combined sources6
Beta strandi139 – 142Combined sources4
Beta strandi144 – 148Combined sources5
Beta strandi151 – 157Combined sources7
Turni162 – 164Combined sources3
Beta strandi169 – 175Combined sources7
Beta strandi185 – 191Combined sources7
Beta strandi194 – 199Combined sources6
Beta strandi204 – 207Combined sources4
Beta strandi211 – 214Combined sources4
Beta strandi218 – 222Combined sources5
Beta strandi232 – 235Combined sources4
Beta strandi238 – 242Combined sources5
Beta strandi244 – 251Combined sources8
Turni252 – 255Combined sources4
Beta strandi256 – 261Combined sources6
Beta strandi270 – 273Combined sources4
Beta strandi280 – 284Combined sources5
Beta strandi289 – 301Combined sources13
Helixi307 – 316Combined sources10
Turni317 – 322Combined sources6
Beta strandi323 – 327Combined sources5
Beta strandi330 – 338Combined sources9
Beta strandi342 – 352Combined sources11
Beta strandi361 – 363Combined sources3
Beta strandi366 – 375Combined sources10
Helixi386 – 394Combined sources9
Turni395 – 397Combined sources3
Helixi412 – 415Combined sources4
Helixi423 – 432Combined sources10
Helixi434 – 442Combined sources9
Turni443 – 445Combined sources3
Beta strandi446 – 448Combined sources3
Helixi452 – 464Combined sources13
Helixi465 – 468Combined sources4
Helixi472 – 484Combined sources13
Beta strandi495 – 500Combined sources6
Beta strandi503 – 509Combined sources7
Helixi510 – 512Combined sources3
Beta strandi516 – 520Combined sources5
Beta strandi526 – 529Combined sources4
Beta strandi531 – 533Combined sources3
Beta strandi542 – 544Combined sources3
Turni546 – 548Combined sources3
Beta strandi551 – 553Combined sources3
Helixi555 – 557Combined sources3
Beta strandi559 – 561Combined sources3
Helixi565 – 573Combined sources9
Beta strandi575 – 577Combined sources3
Turni579 – 581Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J04X-ray3.20A/C1-588[»]
ProteinModelPortaliQ12308.
SMRiQ12308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12308.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni399 – 588Sufficient for SPT15-bindingAdd BLAST190

Phylogenomic databases

HOGENOMiHOG000074727.
InParanoidiQ12308.
KOiK15207.
OMAiGLAWYQT.
OrthoDBiEOG092C131N.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024764. TFIIIC_Znf.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12660. zf-TFIIIC. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequencei

Sequence statusi: Complete.

Q12308-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLKDLLVD RKEFEDWKNN LTWARDGTLY LTTFPDISIG QPKYAKDINC
60 70 80 90 100
NSKNLFHVKE FPLEFENKLD FELAQQNGLL NSQPVCYPRV CKPSPIDDWM
110 120 130 140 150
AVLSNNGNVS VFKDNKMLTN LDSKGNLSSR TYHCFEWNPI ESSIVVGNED
160 170 180 190 200
GELQFFSIRK NSENTPEFYF ESSIRLSDAG SKDWVTHIVW YEDVLVAALS
210 220 230 240 250
NNSVFSMTVS ASSHQPVSRM IQNASRRKIT DLKIVDYKVV LTCPGYVHKI
260 270 280 290 300
DLKNYSISSL KTGSLENFHI IPLNHEKEST ILLMSNKTSY KVLLEDELHV
310 320 330 340 350
TADNIIAPYL EKKFKKWSTI WNEFNNYETT LVIHGISLSP DGYSIAIVYD
360 370 380 390 400
MERVAFKYKI ASEQSFNIMF APLYHTWTIS ERAVGLAWYQ TYQIYNQSLP
410 420 430 440 450
KLPENFSMNK KLLNGNYPIS LDFQSYLNAL MKSEEMRIIM FLNMTIDKPS
460 470 480 490 500
ILSFLEALYE YAINKKSELT NSFDLACVLS IAAILKREAP IYNGTLLMKN
510 520 530 540 550
SFLEETFNLE SFTADPETVT STTNNTWKRC GVTLLPILTT HVKICPVSKQ
560 570 580
RVIDIKRDDL NDYGWFTRGL LERFNEISVY CGTTLEVM
Length:588
Mass (Da):67,683
Last modified:November 1, 1996 - v1
Checksum:iBDBD7BBE947BAC63
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36D → T AA sequence (PubMed:10567530).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95034.1.
Z48483 Genomic DNA. Translation: CAA88379.1.
U33335 Genomic DNA. Translation: AAB68098.1.
BK006949 Genomic DNA. Translation: DAA11421.1.
PIRiS52524.
RefSeqiNP_015318.1. NM_001183821.1.

Genome annotation databases

EnsemblFungiiYPL007C; YPL007C; YPL007C.
GeneIDi856100.
KEGGisce:YPL007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA95034.1.
Z48483 Genomic DNA. Translation: CAA88379.1.
U33335 Genomic DNA. Translation: AAB68098.1.
BK006949 Genomic DNA. Translation: DAA11421.1.
PIRiS52524.
RefSeqiNP_015318.1. NM_001183821.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2J04X-ray3.20A/C1-588[»]
ProteinModelPortaliQ12308.
SMRiQ12308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36170. 15 interactors.
DIPiDIP-6743N.
IntActiQ12308. 7 interactors.
MINTiMINT-642941.

Proteomic databases

MaxQBiQ12308.
PRIDEiQ12308.
TopDownProteomicsiQ12308.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL007C; YPL007C; YPL007C.
GeneIDi856100.
KEGGisce:YPL007C.

Organism-specific databases

EuPathDBiFungiDB:YPL007C.
SGDiS000005928. TFC8.

Phylogenomic databases

HOGENOMiHOG000074727.
InParanoidiQ12308.
KOiK15207.
OMAiGLAWYQT.
OrthoDBiEOG092C131N.

Enzyme and pathway databases

BioCyciYEAST:G3O-33926-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12308.
PROiQ12308.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024764. TFIIIC_Znf.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12660. zf-TFIIIC. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTFC8_YEAST
AccessioniPrimary (citable) accession number: Q12308
Secondary accession number(s): D6W405
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.