Q12306 (SMT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like protein SMT3

Gene names

Name:	SMT3
Ordered Locus Names:	YDR510W
ORF Names:	D9719.15

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not known; suppressor of MIF2 mutations.
Subunit structure	Activated by a E1 ligase composed of AOS1 and UBA2.
Miscellaneous	Present with 2940 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
PTM	Isopeptide bond Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein sumoylation Inferred from direct assay Ref.5. Source: SGD
Cellular_component	condensed nuclear chromosome Inferred from direct assay PubMed 16782016. Source: SGD septin ring Inferred from direct assay PubMed 10364461PubMed 10579719. Source: SGD
Molecular_function	protein tag Inferred from direct assay PubMed 10364461PubMed 10579719. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SGS1	P35187	3	EBI-17490,EBI-17059

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 98

98

Ubiquitin-like protein SMT3

PRO_0000035963

Propeptide

99 – 101

3

PRO_0000035964

Regions

Domain

22 – 98

77

Ubiquitin-like

Amino acid modifications

Modified residue

2

1

Phosphoserine Ref.7 Ref.8

Modified residue

4

1

Phosphoserine Ref.8

Modified residue

32

1

Phosphoserine Ref.9

Modified residue

33

1

Phosphoserine Ref.9

Cross-link

98

Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Beta strand

22 – 29

8

Beta strand

34 – 40

7

Helix

46 – 56

11

Helix

60 – 62

3

Beta strand

64 – 67

4

Beta strand

70 – 72

3

Turn

78 – 82

5

Beta strand

87 – 92

6

Sequences

	Sequence		Length	Mass (Da)
	Q12306 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A2790DE7F315E1A7	FASTA	101	11,597
	10 20 30 40 50 60 MSDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME AFAKRQGKEM 70 80 90 100 DSLRFLYDGI RIQADQTPED LDMEDNDIIE AHREQIGGAT Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Meluh P.B., Koshland D.E. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPH1/YNN214.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer." Johnson E.S., Schwienhorst I., Dohmen R.J., Blobel G. EMBO J. 16:5509-5519(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AOS1 AND UBA2.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY. Strain: ADR376.
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-4, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32 AND SER-33, MASS SPECTROMETRY.
[10]	"Solution structure of a yeast ubiquitin-like protein Smt3: the role of structurally less defined sequences in protein-protein recognitions." Sheng W., Liao X. Protein Sci. 11:1482-1491(2002) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
[11]	"Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast." Mossessova E., Lima C.D. Mol. Cell 5:865-876(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 13-98 IN COMPLEX WITH ULP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U27233 Genomic DNA. Translation: AAB01675.1.
U33057 Genomic DNA. Translation: AAB64951.1.
AY558174 Genomic DNA. Translation: AAS56500.1.
BK006938 Genomic DNA. Translation: DAA12341.1.

PIR

S63999.

RefSeq

NP_010798.1. NM_001180818.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EUV	X-ray	1.60	B	13-98	[»]
1L2N	NMR	-	A	1-101	[»]
2EKE	X-ray	1.90	C/D	13-98	[»]
3PGE	X-ray	2.80	A	1-98	[»]
3QHT	X-ray	2.40	A/B	2-98	[»]
3TIX	X-ray	2.90	A/C	3-98	[»]
3UF8	X-ray	1.50	A	13-98	[»]
3UQA	X-ray	1.55	A	13-98	[»]
3UQB	X-ray	1.90	A	13-98	[»]
3V60	X-ray	2.60	A	20-98	[»]
3V61	X-ray	2.80	A	20-98	[»]
3V62	X-ray	2.90	A/D	20-98	[»]
3VAW	X-ray	1.55	A	13-98	[»]
4FN2	X-ray	1.95	A/B	13-98	[»]
4G50	X-ray	1.75	A/B	13-98	[»]
4GGQ	X-ray	1.95	A/B/C/D	13-98	[»]
4GIV	X-ray	2.45	A/B	13-98	[»]

ProteinModelPortal

Q12306.

SMR

Q12306. Positions 20-98.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1364N.

IntAct

Q12306. 50 interactions.

MINT

MINT-390217.

STRING

4932.YDR510W.

Proteomic databases

PaxDb

Q12306.

PeptideAtlas

Q12306.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDR510W; YDR510W; YDR510W.

GeneID

852122.

KEGG

sce:YDR510W.

Organism-specific databases

CYGD

YDR510w.

SGD

S000002918. SMT3.

Phylogenomic databases

eggNOG

COG5227.

GeneTree

ENSGT00390000018808.

HOGENOM

HOG000207495.

KO

K12160.

OMA

DFNSIAF.

OrthoDB

EOG4K6KF1.

Enzyme and pathway databases

BioCyc

YEAST:G3O-30030-MONOMER.

Gene expression databases

Genevestigator

Q12306.

GermOnline

YDR510W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000626. Ubiquitin.
IPR019955. Ubiquitin_supergroup.
[Graphical view]

Pfam

PF00240. ubiquitin. 1 hit.
[Graphical view]

SMART

SM00213. UBQ. 1 hit.
[Graphical view]

PROSITE

PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q12306.

NextBio

970504.

PMAP-CutDB

Q12306.

Entry information

Entry name

SMT3_YEAST

Accession

Primary (citable) accession number: Q12306
Secondary accession number(s): D6VTD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 119 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program