Reviewed, UniProtKB/Swiss-Prot Q12306 (SMT3_YEAST)

Names and origin

Protein names

Recommended name:
    Ubiquitin-like protein SMT3

Gene names

Name:	SMT3
Ordered Locus Names:	YDR510W
ORF Names:	D9719.15

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Not known; suppressor of MIF2 mutations.
Subunit structure	Activated by a E1 ligase composed of AOS1 and UBA2.
Miscellaneous	Present with 2940 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the ubiquitin family. SUMO subfamily. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
PTM	Isopeptide bond Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protein sumoylation Ref.4 Inferred from direct assay. Source: SGD
Cellular component	condensed nuclear chromosome Inferred from direct assay. Source: SGD septin ring Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.10 Inferred from physical interaction. Source: IntAct protein tag Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P43582	1	EBI-17490,EBI-22766
ATP14	Q12349	1	EBI-17490,EBI-3071
CPR1	P14832	1	EBI-17490,EBI-5463
ECM11	Q04110	1	EBI-17490,EBI-37150
ESS1	P22696	1	EBI-17490,EBI-6679
PHD1	P36093	1	EBI-17490,EBI-13366
RED1	P14291	1	EBI-17490,EBI-14909
SGS1	P35187	1	EBI-17490,EBI-17059
SRP1	Q02821	1	EBI-17490,EBI-1797
TDP1	P38319	1	EBI-17490,EBI-20997
TY2A-C	P25383	1	EBI-17490,EBI-21691
ULP1	Q02724	1	EBI-17490,EBI-20050
URN1	Q06525	1	EBI-17490,EBI-35138

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 98

98

Ubiquitin-like protein SMT3

PRO_0000035963

Propeptide

99 – 101

3

PRO_0000035964

Regions

Domain

22 – 98

77

Ubiquitin-like

Amino acid modifications

Modified residue

2

1

Phosphoserine Ref.6 Ref.7

Modified residue

4

1

Phosphoserine Ref.7

Modified residue

32

1

Phosphoserine Ref.8

Modified residue

33

1

Phosphoserine Ref.8

Cross-link

98

Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

101

Helix Strand Turn

Details...

Beta strand

22 – 32

11

Beta strand

34 – 40

7

Helix

46 – 55

10

Helix

60 – 62

3

Beta strand

63 – 67

5

Turn

78 – 82

5

Beta strand

87 – 93

7

Sequences

	Sequence		Length	Mass (Da)
	Q12306-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A2790DE7F315E1A7	FASTA	101	11,597
	10 20 30 40 50 60 MSDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME AFAKRQGKEM 70 80 90 100 DSLRFLYDGI RIQADQTPED LDMEDNDIIE AHREQIGGAT Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Meluh P.B., Koshland D.E. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YPH1/YNN214.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer." Johnson E.S., Schwienhorst I., Dohmen R.J., Blobel G. EMBO J. 16:5509-5519(1997) [PubMed: 9312010] [Abstract] Cited for: INTERACTION WITH AOS1 AND UBA2.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2 AND SER-4, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32 AND SER-33, MASS SPECTROMETRY.
[9]	"Solution structure of a yeast ubiquitin-like protein Smt3: the role of structurally less defined sequences in protein-protein recognitions." Sheng W., Liao X. Protein Sci. 11:1482-1491(2002) [PubMed: 12021447] [Abstract] Cited for: STRUCTURE BY NMR.
[10]	"Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast." Mossessova E., Lima C.D. Mol. Cell 5:865-876(2000) [PubMed: 10882122] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 13-98 IN COMPLEX WITH ULP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U27233 Genomic DNA. Translation: AAB01675.1.
U33057 Genomic DNA. Translation: AAB64951.1.
AY558174 Genomic DNA. Translation: AAS56500.1.

PIR

S63999.

RefSeq

NP_010798.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EUV	X-ray	1.60	B	13-98	[»]
1L2N	NMR	-	A	1-101	[»]
2EKE	X-ray	1.90	C/D	13-98	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:1364N.

IntAct

Q12306. 30 interactions.

Proteomic databases

PeptideAtlas

Q12306.

Genome annotation databases

Ensembl

YDR510W. Saccharomyces cerevisiae. [Contig view]

GeneID

852122.

GenomeReviews

Gene locus YDR510W in contig Z71256_GR.

KEGG

sce:YDR510W.

NMPDR

fig|4932.3.peg.1571.

Organism-specific databases

CYGD

YDR510w.

SGD

S000002918. SMT3.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

Q12306.

OMA

Q12306. QGKEMDS.

Gene expression databases

GermOnline

YDR510W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000626. Ubiquitin.
IPR019955. Ubiquitin_supergroup.
[Graphical view]

Pfam

PF00240. ubiquitin. 1 hit.
[Graphical view]

SMART

SM00213. UBQ. 1 hit.
[Graphical view]

PROSITE

PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

970504.

PMAP-CutDB

Q12306.

Entry information

Entry name

SMT3_YEAST

Accession

Primary (citable) accession number: Q12306

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)