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Protein

Ubiquitin-like protein SMT3

Gene

SMT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Not known; suppressor of MIF2 mutations.

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein tag Source: SGD

GO - Biological processi

  • mitotic sister chromatid segregation Source: GO_Central
  • protein sumoylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-30030-MONOMER.
ReactomeiR-SCE-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-SCE-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).
R-SCE-3065679. SUMO is proteolytically processed.
R-SCE-3108214. SUMOylation of DNA damage response and repair proteins.
R-SCE-4570464. SUMOylation of RNA binding proteins.
R-SCE-4615885. SUMOylation of DNA replication proteins.
R-SCE-5696395. Formation of Incision Complex in GG-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein SMT3
Gene namesi
Name:SMT3
Ordered Locus Names:YDR510W
ORF Names:D9719.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR510W.
SGDiS000002918. SMT3.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome Source: SGD
  • nucleus Source: SGD
  • septin ring Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 9897Ubiquitin-like protein SMT3PRO_0000035963Add
BLAST
Propeptidei99 – 1013PRO_0000035964

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei2 – 21PhosphoserineCombined sources
Modified residuei4 – 41PhosphoserineCombined sources
Cross-linki98 – 98Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein

Proteomic databases

MaxQBiQ12306.

PTM databases

iPTMnetiQ12306.

Miscellaneous databases

PMAP-CutDBQ12306.

Interactioni

Subunit structurei

Activated by a E1 ligase composed of AOS1 and UBA2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-17490,EBI-17490
ELG1Q1205011EBI-17490,EBI-32195
POL30P158736EBI-17490,EBI-12993
RED1P142916EBI-17490,EBI-14909
SGS1P351873EBI-17490,EBI-17059
SRS2P129544EBI-17490,EBI-18110

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi32561. 433 interactions.
DIPiDIP-1364N.
IntActiQ12306. 50 interactions.
MINTiMINT-390217.

Structurei

Secondary structure

1
101
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 298Combined sources
Beta strandi34 – 407Combined sources
Helixi46 – 5611Combined sources
Helixi60 – 623Combined sources
Beta strandi64 – 674Combined sources
Beta strandi70 – 723Combined sources
Turni78 – 825Combined sources
Beta strandi87 – 926Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUVX-ray1.60B13-98[»]
1L2NNMR-A1-101[»]
2EKEX-ray1.90C/D13-98[»]
3PGEX-ray2.80A1-98[»]
3QHTX-ray2.40A/B2-98[»]
3TIXX-ray2.90A/C1-98[»]
3UF8X-ray1.50A13-98[»]
3UQAX-ray1.55A13-98[»]
3UQBX-ray1.90A13-98[»]
3V60X-ray2.60A20-98[»]
3V61X-ray2.80A20-98[»]
3V62X-ray2.90A/D20-98[»]
3VAWX-ray1.55A13-98[»]
4FN2X-ray1.95A/B13-98[»]
4G50X-ray1.75A/B13-98[»]
4GGQX-ray1.95A/B/C/D13-98[»]
4GIVX-ray2.45A/B13-98[»]
5D6JX-ray2.25B21-94[»]
ProteinModelPortaliQ12306.
SMRiQ12306. Positions 20-98.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12306.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 9877Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin family. SUMO subfamily.Curated
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000018808.
HOGENOMiHOG000207495.
InParanoidiQ12306.
KOiK12160.
OMAiHREQVGG.
OrthoDBiEOG092C5C59.

Family and domain databases

InterProiIPR022617. Rad60/SUMO-like_dom.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSEVNQEA KPEVKPEVKP ETHINLKVSD GSSEIFFKIK KTTPLRRLME
60 70 80 90 100
AFAKRQGKEM DSLRFLYDGI RIQADQTPED LDMEDNDIIE AHREQIGGAT

Y
Length:101
Mass (Da):11,597
Last modified:November 1, 1996 - v1
Checksum:iA2790DE7F315E1A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27233 Genomic DNA. Translation: AAB01675.1.
U33057 Genomic DNA. Translation: AAB64951.1.
AY558174 Genomic DNA. Translation: AAS56500.1.
BK006938 Genomic DNA. Translation: DAA12341.1.
PIRiS63999.
RefSeqiNP_010798.1. NM_001180818.1.

Genome annotation databases

EnsemblFungiiYDR510W; YDR510W; YDR510W.
GeneIDi852122.
KEGGisce:YDR510W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27233 Genomic DNA. Translation: AAB01675.1.
U33057 Genomic DNA. Translation: AAB64951.1.
AY558174 Genomic DNA. Translation: AAS56500.1.
BK006938 Genomic DNA. Translation: DAA12341.1.
PIRiS63999.
RefSeqiNP_010798.1. NM_001180818.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUVX-ray1.60B13-98[»]
1L2NNMR-A1-101[»]
2EKEX-ray1.90C/D13-98[»]
3PGEX-ray2.80A1-98[»]
3QHTX-ray2.40A/B2-98[»]
3TIXX-ray2.90A/C1-98[»]
3UF8X-ray1.50A13-98[»]
3UQAX-ray1.55A13-98[»]
3UQBX-ray1.90A13-98[»]
3V60X-ray2.60A20-98[»]
3V61X-ray2.80A20-98[»]
3V62X-ray2.90A/D20-98[»]
3VAWX-ray1.55A13-98[»]
4FN2X-ray1.95A/B13-98[»]
4G50X-ray1.75A/B13-98[»]
4GGQX-ray1.95A/B/C/D13-98[»]
4GIVX-ray2.45A/B13-98[»]
5D6JX-ray2.25B21-94[»]
ProteinModelPortaliQ12306.
SMRiQ12306. Positions 20-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32561. 433 interactions.
DIPiDIP-1364N.
IntActiQ12306. 50 interactions.
MINTiMINT-390217.

PTM databases

iPTMnetiQ12306.

Proteomic databases

MaxQBiQ12306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR510W; YDR510W; YDR510W.
GeneIDi852122.
KEGGisce:YDR510W.

Organism-specific databases

EuPathDBiFungiDB:YDR510W.
SGDiS000002918. SMT3.

Phylogenomic databases

GeneTreeiENSGT00390000018808.
HOGENOMiHOG000207495.
InParanoidiQ12306.
KOiK12160.
OMAiHREQVGG.
OrthoDBiEOG092C5C59.

Enzyme and pathway databases

BioCyciYEAST:G3O-30030-MONOMER.
ReactomeiR-SCE-3065676. SUMO is conjugated to E1 (UBA2:SAE1).
R-SCE-3065678. SUMO is transferred from E1 to E2 (UBE2I, UBC9).
R-SCE-3065679. SUMO is proteolytically processed.
R-SCE-3108214. SUMOylation of DNA damage response and repair proteins.
R-SCE-4570464. SUMOylation of RNA binding proteins.
R-SCE-4615885. SUMOylation of DNA replication proteins.
R-SCE-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

EvolutionaryTraceiQ12306.
PMAP-CutDBQ12306.
PROiQ12306.

Family and domain databases

InterProiIPR022617. Rad60/SUMO-like_dom.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF11976. Rad60-SLD. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSMT3_YEAST
AccessioniPrimary (citable) accession number: Q12306
Secondary accession number(s): D6VTD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2940 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.