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Protein

Aspartic proteinase yapsin-3

Gene

YPS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves proteins C-terminally to mono- and paired-basic residues. Required for cell wall integrity.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811PROSITE-ProRule annotation
Active sitei288 – 2881PROSITE-ProRule annotation

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
  • protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32266-MONOMER.

Protein family/group databases

MEROPSiA01.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase yapsin-3 (EC:3.4.23.-)
Gene namesi
Name:YPS3
Ordered Locus Names:YLR121C
ORF Names:L2964, L9233.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR121C.
SGDiS000004111. YPS3.

Subcellular locationi

GO - Cellular componenti

  • anchored component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 47271 PublicationPRO_0000025841Add
BLAST
Chaini48 – 483436Aspartic proteinase yapsin-3PRO_0000025842Add
BLAST
Propeptidei484 – 50825Removed in mature formSequence analysisPRO_0000025843Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Glycosylationi160 – 1601N-linked (GlcNAc...)Sequence analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence analysis
Glycosylationi275 – 2751N-linked (GlcNAc...)Sequence analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence analysis
Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence analysis
Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence analysis
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence analysis
Glycosylationi462 – 4621N-linked (GlcNAc...)Sequence analysis
Lipidationi483 – 4831GPI-anchor amidated asparagineSequence analysis

Post-translational modificationi

Can also be processed to start at Phe-54.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

MaxQBiQ12303.

Expressioni

Inductioni

Positively regulated in response to cell wall perturbation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31393. 9 interactions.
DIPiDIP-4569N.
IntActiQ12303. 1 interaction.
MINTiMINT-405669.

Structurei

3D structure databases

ProteinModelPortaliQ12303.
SMRiQ12303. Positions 56-400.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 394332Peptidase A1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00550000075429.
HOGENOMiHOG000248646.
InParanoidiQ12303.
OMAiIGYSANE.
OrthoDBiEOG092C3KPP.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQLAAVAT LAVLTSPAFG RVLPDGKYVK IPFTKKKNGD NGELSKRSNG
60 70 80 90 100
HEKFVLANEQ SFYSVELAIG TPSQNLTVLL DTGSADLWVP GKGNPYCGSV
110 120 130 140 150
MDCDQYGVFD KTKSSTFKAN KSSPFYAAYG DGTYAEGAFG QDKLKYNELD
160 170 180 190 200
LSGLSFAVAN ESNSTFGVLG IGLSTLEVTY SGKVAIMDKR SYEYDNFPLF
210 220 230 240 250
LKHSGAIDAT AYSLFLNDES QSSGSILFGA VDHSKYEGQL YTIPLVNLYK
260 270 280 290 300
SQGYQHPVAF DVTLQGLGLQ TDKRNITLTT TKLPALLDSG TTLTYLPSQA
310 320 330 340 350
VALLAKSLNA SYSKTLGYYE YTCPSSDNKT SVAFDFGGFR INAPLSDFTM
360 370 380 390 400
QTSVGTCVLA IIPQAGNATA ILGDSFLRNA YVVYDLDNYE ISLAQAKYGT
410 420 430 440 450
GKENVEVIKS TVPSAIRAPS YNNTWSNYAS ATSGGNIFTT VRTFNGTSTA
460 470 480 490 500
TTTRSTTTKK TNSTTTAKST HKSKRALQRA ATNSASSIRS TLGLLLVPSL

LILSVFFS
Length:508
Mass (Da):54,570
Last modified:November 1, 1996 - v1
Checksum:i552E4B236FF12ABA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61700.1.
Z73293 Genomic DNA. Translation: CAA97689.1.
U53877 Genomic DNA. Translation: AAB82368.1.
BK006945 Genomic DNA. Translation: DAA09435.1.
PIRiS64958.
RefSeqiNP_013222.1. NM_001182008.1.

Genome annotation databases

EnsemblFungiiYLR121C; YLR121C; YLR121C.
GeneIDi850812.
KEGGisce:YLR121C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89514 Genomic DNA. Translation: CAA61700.1.
Z73293 Genomic DNA. Translation: CAA97689.1.
U53877 Genomic DNA. Translation: AAB82368.1.
BK006945 Genomic DNA. Translation: DAA09435.1.
PIRiS64958.
RefSeqiNP_013222.1. NM_001182008.1.

3D structure databases

ProteinModelPortaliQ12303.
SMRiQ12303. Positions 56-400.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31393. 9 interactions.
DIPiDIP-4569N.
IntActiQ12303. 1 interaction.
MINTiMINT-405669.

Protein family/group databases

MEROPSiA01.035.

Proteomic databases

MaxQBiQ12303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR121C; YLR121C; YLR121C.
GeneIDi850812.
KEGGisce:YLR121C.

Organism-specific databases

EuPathDBiFungiDB:YLR121C.
SGDiS000004111. YPS3.

Phylogenomic databases

GeneTreeiENSGT00550000075429.
HOGENOMiHOG000248646.
InParanoidiQ12303.
OMAiIGYSANE.
OrthoDBiEOG092C3KPP.

Enzyme and pathway databases

BioCyciYEAST:G3O-32266-MONOMER.

Miscellaneous databases

PROiQ12303.

Family and domain databases

Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiYPS3_YEAST
AccessioniPrimary (citable) accession number: Q12303
Secondary accession number(s): D6VYB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.