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Protein

High-affinity glucose transporter RGT2

Gene

RGT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Low-affinity glucose transporter. Can function as a sensor that generates an intracellular signal in the presence of high level of glucose. Required for maximal expression of the high glucose-induced HXT1 protein.

GO - Molecular functioni

  • glucose binding Source: SGD
  • receptor activity Source: SGD
  • substrate-specific transmembrane transporter activity Source: InterPro

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • detection of glucose Source: SGD
  • signal transduction Source: SGD
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-29537-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.19. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity glucose transporter RGT2
Gene namesi
Name:RGT2
Ordered Locus Names:YDL138W
ORF Names:D2160
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDL138w.
EuPathDBiFungiDB:YDL138W.
SGDiS000002297. RGT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei100 – 12021Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini121 – 14424ExtracellularSequence AnalysisAdd
BLAST
Transmembranei145 – 16521Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini166 – 17510CytoplasmicSequence Analysis
Transmembranei176 – 19621Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini197 – 1971ExtracellularSequence Analysis
Transmembranei198 – 21821Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini219 – 23113CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei232 – 25221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini253 – 26614ExtracellularSequence AnalysisAdd
BLAST
Transmembranei267 – 28721Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini288 – 35770CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei358 – 37821Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini379 – 39315ExtracellularSequence AnalysisAdd
BLAST
Transmembranei394 – 41421Helical; Name=8Sequence AnalysisAdd
BLAST
Topological domaini415 – 4217CytoplasmicSequence Analysis
Transmembranei422 – 44221Helical; Name=9Sequence AnalysisAdd
BLAST
Topological domaini443 – 45210ExtracellularSequence Analysis
Transmembranei453 – 47321Helical; Name=10Sequence AnalysisAdd
BLAST
Topological domaini474 – 49118CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei492 – 51221Helical; Name=11Sequence AnalysisAdd
BLAST
Topological domaini513 – 52412ExtracellularSequence AnalysisAdd
BLAST
Transmembranei525 – 54521Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini546 – 763218CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi231 – 2311R → K in RGT2-1; constitutively signaling glucose receptor.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 763763High-affinity glucose transporter RGT2PRO_0000050389Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriQ12300.

Interactioni

Protein-protein interaction databases

BioGridi31923. 13 interactions.
DIPiDIP-2865N.
IntActiQ12300. 15 interactions.
MINTiMINT-508420.
STRINGi4932.YDL138W.

Structurei

3D structure databases

ProteinModelPortaliQ12300.
SMRiQ12300. Positions 102-559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiQ12300.
KOiK08139.
OMAiIMIAFIC.
OrthoDBiEOG73JM4H.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12300-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDSQNCLRQ REENSHLNPG NDFGHHQGAE CTINHNNMPH RNAYTESTND
60 70 80 90 100
TEAKSIVMCD DPNAYQISYT NNEPAGDGAI ETTSILLSQP LPLRSNVMSV
110 120 130 140 150
LVGIFVAVGG FLFGYDTGLI NSITDMPYVK TYIAPNHSYF TTSQIAILVS
160 170 180 190 200
FLSLGTFFGA LIAPYISDSY GRKPTIMFST AVIFSIGNSL QVASGGLVLL
210 220 230 240 250
IVGRVISGIG IGIISAVVPL YQAEAAQKNL RGAIISSYQW AITIGLLVSS
260 270 280 290 300
AVSQGTHSKN GPSSYRIPIG LQYVWSSILA VGMIFLPESP RYYVLKDELN
310 320 330 340 350
KAAKSLSFLR GLPIEDPRLL EELVEIKATY DYEASFGPST LLDCFKTSEN
360 370 380 390 400
RPKQILRIFT GIAIQAFQQA SGINFIFYYG VNFFNNTGVD NSYLVSFISY
410 420 430 440 450
AVNVAFSIPG MYLVDRIGRR PVLLAGGVIM AIANLVIAIV GVSEGKTVVA
460 470 480 490 500
SKIMIAFICL FIAAFSATWG GVVWVVSAEL YPLGVRSKCT AICAAANWLV
510 520 530 540 550
NFTCALITPY IVDVGSHTSS MGPKIFFIWG GLNVVAVIVV YFAVYETRGL
560 570 580 590 600
TLEEIDELFR KAPNSVISSK WNKKIRKRCL AFPISQQIEM KTNIKNAGKL
610 620 630 640 650
DNNNSPIVQD DSHNIIDVDG FLENQIQSND HMIAADKGSG SLVNIIDTAP
660 670 680 690 700
LTSTEFKPVE HPPVNYVDLG NGLGLNTYNR GPPSIISDST DEFYEENDSS
710 720 730 740 750
YYNNNTERNG ANSVNTYMAQ LINSSSTTSN DTSFSPSHNS NARTSSNWTS
760
DLASKHSQYT SPQ
Length:763
Mass (Da):83,159
Last modified:November 1, 1997 - v1
Checksum:iDF6E2F1C337F106E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96876 Genomic DNA. Translation: CAA65621.1.
Z74186 Genomic DNA. Translation: CAA98711.1.
BK006938 Genomic DNA. Translation: DAA11720.1.
PIRiS67684.
RefSeqiNP_010143.1. NM_001180198.1.

Genome annotation databases

EnsemblFungiiYDL138W; YDL138W; YDL138W.
GeneIDi851417.
KEGGisce:YDL138W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96876 Genomic DNA. Translation: CAA65621.1.
Z74186 Genomic DNA. Translation: CAA98711.1.
BK006938 Genomic DNA. Translation: DAA11720.1.
PIRiS67684.
RefSeqiNP_010143.1. NM_001180198.1.

3D structure databases

ProteinModelPortaliQ12300.
SMRiQ12300. Positions 102-559.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31923. 13 interactions.
DIPiDIP-2865N.
IntActiQ12300. 15 interactions.
MINTiMINT-508420.
STRINGi4932.YDL138W.

Protein family/group databases

TCDBi2.A.1.1.19. the major facilitator superfamily (mfs).

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL138W; YDL138W; YDL138W.
GeneIDi851417.
KEGGisce:YDL138W.

Organism-specific databases

CYGDiYDL138w.
EuPathDBiFungiDB:YDL138W.
SGDiS000002297. RGT2.

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiQ12300.
KOiK08139.
OMAiIMIAFIC.
OrthoDBiEOG73JM4H.

Enzyme and pathway databases

BioCyciYEAST:G3O-29537-MONOMER.

Miscellaneous databases

NextBioi968612.
PROiQ12300.

Gene expression databases

GenevestigatoriQ12300.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
    Woelfl S., Haneman V., Saluz H.P.
    Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression."
    Oezcan S., Dover J., Rosenwald A.G., Woelfl S., Johnston M.
    Proc. Natl. Acad. Sci. U.S.A. 93:12428-12432(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiRGT2_YEAST
AccessioniPrimary (citable) accession number: Q12300
Secondary accession number(s): D6VRL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 29, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.