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Q12300 (RGT2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High-affinity glucose transporter RGT2
Gene names
Name:RGT2
Ordered Locus Names:YDL138W
ORF Names:D2160
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Low-affinity glucose transporter. Can function as a sensor that generates an intracellular signal in the presence of high level of glucose. Required for maximal expression of the high glucose-induced HXT1 protein.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 763763High-affinity glucose transporter RGT2
PRO_0000050389

Regions

Topological domain1 – 9999Cytoplasmic Potential
Transmembrane100 – 12021Helical; Name=1; Potential
Topological domain121 – 14424Extracellular Potential
Transmembrane145 – 16521Helical; Name=2; Potential
Topological domain166 – 17510Cytoplasmic Potential
Transmembrane176 – 19621Helical; Name=3; Potential
Topological domain1971Extracellular Potential
Transmembrane198 – 21821Helical; Name=4; Potential
Topological domain219 – 23113Cytoplasmic Potential
Transmembrane232 – 25221Helical; Name=5; Potential
Topological domain253 – 26614Extracellular Potential
Transmembrane267 – 28721Helical; Name=6; Potential
Topological domain288 – 35770Cytoplasmic Potential
Transmembrane358 – 37821Helical; Name=7; Potential
Topological domain379 – 39315Extracellular Potential
Transmembrane394 – 41421Helical; Name=8; Potential
Topological domain415 – 4217Cytoplasmic Potential
Transmembrane422 – 44221Helical; Name=9; Potential
Topological domain443 – 45210Extracellular Potential
Transmembrane453 – 47321Helical; Name=10; Potential
Topological domain474 – 49118Cytoplasmic Potential
Transmembrane492 – 51221Helical; Name=11; Potential
Topological domain513 – 52412Extracellular Potential
Transmembrane525 – 54521Helical; Name=12; Potential
Topological domain546 – 763218Cytoplasmic Potential

Amino acid modifications

Glycosylation1361N-linked (GlcNAc...) Potential
Glycosylation3851N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2311R → K in RGT2-1; constitutively signaling glucose receptor.

Sequences

Sequence LengthMass (Da)Tools
Q12300 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: DF6E2F1C337F106E

FASTA76383,159
        10         20         30         40         50         60 
MNDSQNCLRQ REENSHLNPG NDFGHHQGAE CTINHNNMPH RNAYTESTND TEAKSIVMCD 

        70         80         90        100        110        120 
DPNAYQISYT NNEPAGDGAI ETTSILLSQP LPLRSNVMSV LVGIFVAVGG FLFGYDTGLI 

       130        140        150        160        170        180 
NSITDMPYVK TYIAPNHSYF TTSQIAILVS FLSLGTFFGA LIAPYISDSY GRKPTIMFST 

       190        200        210        220        230        240 
AVIFSIGNSL QVASGGLVLL IVGRVISGIG IGIISAVVPL YQAEAAQKNL RGAIISSYQW 

       250        260        270        280        290        300 
AITIGLLVSS AVSQGTHSKN GPSSYRIPIG LQYVWSSILA VGMIFLPESP RYYVLKDELN 

       310        320        330        340        350        360 
KAAKSLSFLR GLPIEDPRLL EELVEIKATY DYEASFGPST LLDCFKTSEN RPKQILRIFT 

       370        380        390        400        410        420 
GIAIQAFQQA SGINFIFYYG VNFFNNTGVD NSYLVSFISY AVNVAFSIPG MYLVDRIGRR 

       430        440        450        460        470        480 
PVLLAGGVIM AIANLVIAIV GVSEGKTVVA SKIMIAFICL FIAAFSATWG GVVWVVSAEL 

       490        500        510        520        530        540 
YPLGVRSKCT AICAAANWLV NFTCALITPY IVDVGSHTSS MGPKIFFIWG GLNVVAVIVV 

       550        560        570        580        590        600 
YFAVYETRGL TLEEIDELFR KAPNSVISSK WNKKIRKRCL AFPISQQIEM KTNIKNAGKL 

       610        620        630        640        650        660 
DNNNSPIVQD DSHNIIDVDG FLENQIQSND HMIAADKGSG SLVNIIDTAP LTSTEFKPVE 

       670        680        690        700        710        720 
HPPVNYVDLG NGLGLNTYNR GPPSIISDST DEFYEENDSS YYNNNTERNG ANSVNTYMAQ 

       730        740        750        760 
LINSSSTTSN DTSFSPSHNS NARTSSNWTS DLASKHSQYT SPQ 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
Woelfl S., Haneman V., Saluz H.P.
Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression."
Oezcan S., Dover J., Rosenwald A.G., Woelfl S., Johnston M.
Proc. Natl. Acad. Sci. U.S.A. 93:12428-12432(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X96876 Genomic DNA. Translation: CAA65621.1.
Z74186 Genomic DNA. Translation: CAA98711.1.
BK006938 Genomic DNA. Translation: DAA11720.1.
PIRS67684.
RefSeqNP_010143.1. NM_001180198.1.

3D structure databases

ProteinModelPortalQ12300.
SMRQ12300. Positions 102-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31923. 13 interactions.
DIPDIP-2865N.
IntActQ12300. 15 interactions.
MINTMINT-508420.
STRING4932.YDL138W.

Protein family/group databases

TCDB2.A.1.1.19. the major facilitator superfamily (mfs).

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL138W; YDL138W; YDL138W.
GeneID851417.
KEGGsce:YDL138W.

Organism-specific databases

CYGDYDL138w.
SGDS000002297. RGT2.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00720000108754.
HOGENOMHOG000202870.
KOK08139.
OMAWLVNFVC.
OrthoDBEOG73JM4H.

Enzyme and pathway databases

BioCycYEAST:G3O-29537-MONOMER.

Gene expression databases

GenevestigatorQ12300.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR00171. SUGRTRNSPORT.
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968612.
PROQ12300.

Entry information

Entry nameRGT2_YEAST
AccessionPrimary (citable) accession number: Q12300
Secondary accession number(s): D6VRL0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 19, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families