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Q12300

- RGT2_YEAST

UniProt

Q12300 - RGT2_YEAST

Protein

High-affinity glucose transporter RGT2

Gene

RGT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Low-affinity glucose transporter. Can function as a sensor that generates an intracellular signal in the presence of high level of glucose. Required for maximal expression of the high glucose-induced HXT1 protein.

    GO - Molecular functioni

    1. glucose binding Source: SGD
    2. receptor activity Source: SGD
    3. substrate-specific transmembrane transporter activity Source: InterPro

    GO - Biological processi

    1. carbohydrate transport Source: UniProtKB-KW
    2. detection of glucose Source: SGD
    3. signal transduction Source: SGD

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29537-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.1.19. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High-affinity glucose transporter RGT2
    Gene namesi
    Name:RGT2
    Ordered Locus Names:YDL138W
    ORF Names:D2160
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL138w.
    SGDiS000002297. RGT2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi231 – 2311R → K in RGT2-1; constitutively signaling glucose receptor.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 763763High-affinity glucose transporter RGT2PRO_0000050389Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriQ12300.

    Interactioni

    Protein-protein interaction databases

    BioGridi31923. 13 interactions.
    DIPiDIP-2865N.
    IntActiQ12300. 15 interactions.
    MINTiMINT-508420.
    STRINGi4932.YDL138W.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12300.
    SMRiQ12300. Positions 102-559.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini121 – 14424ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini166 – 17510CytoplasmicSequence Analysis
    Topological domaini197 – 1971ExtracellularSequence Analysis
    Topological domaini219 – 23113CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini253 – 26614ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini288 – 35770CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini379 – 39315ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini415 – 4217CytoplasmicSequence Analysis
    Topological domaini443 – 45210ExtracellularSequence Analysis
    Topological domaini474 – 49118CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini513 – 52412ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini546 – 763218CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei100 – 12021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei145 – 16521Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei176 – 19621Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei198 – 21821Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei358 – 37821Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei394 – 41421Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei422 – 44221Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei453 – 47321Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei492 – 51221Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei525 – 54521Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00720000108754.
    HOGENOMiHOG000202870.
    KOiK08139.
    OMAiWLVNFVC.
    OrthoDBiEOG73JM4H.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12300-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDSQNCLRQ REENSHLNPG NDFGHHQGAE CTINHNNMPH RNAYTESTND    50
    TEAKSIVMCD DPNAYQISYT NNEPAGDGAI ETTSILLSQP LPLRSNVMSV 100
    LVGIFVAVGG FLFGYDTGLI NSITDMPYVK TYIAPNHSYF TTSQIAILVS 150
    FLSLGTFFGA LIAPYISDSY GRKPTIMFST AVIFSIGNSL QVASGGLVLL 200
    IVGRVISGIG IGIISAVVPL YQAEAAQKNL RGAIISSYQW AITIGLLVSS 250
    AVSQGTHSKN GPSSYRIPIG LQYVWSSILA VGMIFLPESP RYYVLKDELN 300
    KAAKSLSFLR GLPIEDPRLL EELVEIKATY DYEASFGPST LLDCFKTSEN 350
    RPKQILRIFT GIAIQAFQQA SGINFIFYYG VNFFNNTGVD NSYLVSFISY 400
    AVNVAFSIPG MYLVDRIGRR PVLLAGGVIM AIANLVIAIV GVSEGKTVVA 450
    SKIMIAFICL FIAAFSATWG GVVWVVSAEL YPLGVRSKCT AICAAANWLV 500
    NFTCALITPY IVDVGSHTSS MGPKIFFIWG GLNVVAVIVV YFAVYETRGL 550
    TLEEIDELFR KAPNSVISSK WNKKIRKRCL AFPISQQIEM KTNIKNAGKL 600
    DNNNSPIVQD DSHNIIDVDG FLENQIQSND HMIAADKGSG SLVNIIDTAP 650
    LTSTEFKPVE HPPVNYVDLG NGLGLNTYNR GPPSIISDST DEFYEENDSS 700
    YYNNNTERNG ANSVNTYMAQ LINSSSTTSN DTSFSPSHNS NARTSSNWTS 750
    DLASKHSQYT SPQ 763
    Length:763
    Mass (Da):83,159
    Last modified:November 1, 1997 - v1
    Checksum:iDF6E2F1C337F106E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96876 Genomic DNA. Translation: CAA65621.1.
    Z74186 Genomic DNA. Translation: CAA98711.1.
    BK006938 Genomic DNA. Translation: DAA11720.1.
    PIRiS67684.
    RefSeqiNP_010143.1. NM_001180198.1.

    Genome annotation databases

    EnsemblFungiiYDL138W; YDL138W; YDL138W.
    GeneIDi851417.
    KEGGisce:YDL138W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X96876 Genomic DNA. Translation: CAA65621.1 .
    Z74186 Genomic DNA. Translation: CAA98711.1 .
    BK006938 Genomic DNA. Translation: DAA11720.1 .
    PIRi S67684.
    RefSeqi NP_010143.1. NM_001180198.1.

    3D structure databases

    ProteinModelPortali Q12300.
    SMRi Q12300. Positions 102-559.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31923. 13 interactions.
    DIPi DIP-2865N.
    IntActi Q12300. 15 interactions.
    MINTi MINT-508420.
    STRINGi 4932.YDL138W.

    Protein family/group databases

    TCDBi 2.A.1.1.19. the major facilitator superfamily (mfs).

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL138W ; YDL138W ; YDL138W .
    GeneIDi 851417.
    KEGGi sce:YDL138W.

    Organism-specific databases

    CYGDi YDL138w.
    SGDi S000002297. RGT2.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00720000108754.
    HOGENOMi HOG000202870.
    KOi K08139.
    OMAi WLVNFVC.
    OrthoDBi EOG73JM4H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29537-MONOMER.

    Miscellaneous databases

    NextBioi 968612.
    PROi Q12300.

    Gene expression databases

    Genevestigatori Q12300.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    PRINTSi PR00171. SUGRTRNSPORT.
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00879. SP. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
      Woelfl S., Haneman V., Saluz H.P.
      Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression."
      Oezcan S., Dover J., Rosenwald A.G., Woelfl S., Johnston M.
      Proc. Natl. Acad. Sci. U.S.A. 93:12428-12432(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiRGT2_YEAST
    AccessioniPrimary (citable) accession number: Q12300
    Secondary accession number(s): D6VRL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3