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Q12297

- TAF3_YEAST

UniProt

Q12297 - TAF3_YEAST

Protein

Transcription initiation factor TFIID subunit 3

Gene

TAF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.6 Publications

    GO - Molecular functioni

    1. chromatin binding Source: SGD

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
    3. transcription from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33930-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor TFIID subunit 3
    Alternative name(s):
    TAFII-47
    Short name:
    TAFII47
    TBP-associated factor 3
    TBP-associated factor 47 kDa
    Gene namesi
    Name:TAF3
    Synonyms:TAF47
    Ordered Locus Names:YPL011C
    ORF Names:LPA6C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL011c.
    SGDiS000005932. TAF3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. transcription factor TFIID complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 353353Transcription initiation factor TFIID subunit 3PRO_0000118868Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei237 – 2371Phosphothreonine1 Publication
    Modified residuei310 – 3101Phosphoserine2 Publications
    Modified residuei346 – 3461Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ12297.
    PaxDbiQ12297.
    PeptideAtlasiQ12297.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12297.

    Interactioni

    Subunit structurei

    TAF3 heterodimerizes with TAF10. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

    Protein-protein interaction databases

    BioGridi36166. 28 interactions.
    DIPiDIP-5328N.
    IntActiQ12297. 18 interactions.
    MINTiMINT-545815.
    STRINGi4932.YPL011C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 7868Histone-foldAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili289 – 30921Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TAF3 family.Curated
    Contains 1 histone-fold domain.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG70559.
    HOGENOMiHOG000141699.
    KOiK14650.
    OMAiEAMINNG.
    OrthoDBiEOG78D7Z5.

    Family and domain databases

    InterProiIPR006565. BTP.
    [Graphical view]
    PfamiPF07524. Bromo_TP. 1 hit.
    [Graphical view]
    SMARTiSM00576. BTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12297-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTNNDFYFA LLRISILQLL KAQGFDRARP SLVDVMTDLY AKFLSLLASE    50
    VSSIAQARCD QDDTIALQDI TLALENLGIV KPTNVLDVYD ENSELSSSRG 100
    MEKFKDWCIY STQLTDARIT ALPTVELLQS EEKESDPLSA IPDYLNQLLQ 150
    NKGAKQKLET KNRKTELIED LINNNGLDDW IKLVIARQRI NMIERASKKE 200
    SQNVPALPHI AGYKSSILSR HHHTTITNED RMPSAMTPRD EDALTEIQEN 250
    PFVTSKLPIM RKENRLENIT LSFEDEELES LGEVEGPNQK SQENNNEESF 300
    KENNKSLTES PHGDDRDISM FQFDSNVDTK WAEQEDMDST FQRRTSLDYG 350
    GYF 353
    Length:353
    Mass (Da):40,296
    Last modified:November 1, 1996 - v1
    Checksum:i98A6B7813A29978F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33335 Genomic DNA. Translation: AAB68094.1.
    Z71255 Genomic DNA. Translation: CAA95030.1.
    Z48483 Genomic DNA. Translation: CAA88375.1.
    BK006949 Genomic DNA. Translation: DAA11417.1.
    PIRiS52520.
    RefSeqiNP_015314.1. NM_001183825.1.

    Genome annotation databases

    EnsemblFungiiYPL011C; YPL011C; YPL011C.
    GeneIDi856096.
    KEGGisce:YPL011C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U33335 Genomic DNA. Translation: AAB68094.1 .
    Z71255 Genomic DNA. Translation: CAA95030.1 .
    Z48483 Genomic DNA. Translation: CAA88375.1 .
    BK006949 Genomic DNA. Translation: DAA11417.1 .
    PIRi S52520.
    RefSeqi NP_015314.1. NM_001183825.1.

    3D structure databases

    ProteinModelPortali Q12297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36166. 28 interactions.
    DIPi DIP-5328N.
    IntActi Q12297. 18 interactions.
    MINTi MINT-545815.
    STRINGi 4932.YPL011C.

    Proteomic databases

    MaxQBi Q12297.
    PaxDbi Q12297.
    PeptideAtlasi Q12297.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL011C ; YPL011C ; YPL011C .
    GeneIDi 856096.
    KEGGi sce:YPL011C.

    Organism-specific databases

    CYGDi YPL011c.
    SGDi S000005932. TAF3.

    Phylogenomic databases

    eggNOGi NOG70559.
    HOGENOMi HOG000141699.
    KOi K14650.
    OMAi EAMINNG.
    OrthoDBi EOG78D7Z5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33930-MONOMER.

    Miscellaneous databases

    NextBioi 981134.

    Gene expression databases

    Genevestigatori Q12297.

    Family and domain databases

    InterProi IPR006565. BTP.
    [Graphical view ]
    Pfami PF07524. Bromo_TP. 1 hit.
    [Graphical view ]
    SMARTi SM00576. BTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M."
      Gerring S.L., Spencer F., Hieter P.
      EMBO J. 9:4347-4358(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain."
      O'Hara P.J., Horowitz H., Eichinger G., Young E.T.
      Nucleic Acids Res. 16:10153-10169(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
      Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
      Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
    6. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
      Sanders S.L., Weil P.A.
      J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    7. "Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
      Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
      Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
    8. "The histone fold is a key structural motif of transcription factor TFIID."
      Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
      Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
    9. "Molecular characterization of Saccharomyces cerevisiae TFIID."
      Sanders S.L., Garbett K.A., Weil P.A.
      Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TFIID STOICHIOMETRY.
    10. "Multi-protein complexes in eukaryotic gene transcription."
      Martinez E.
      Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    13. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
    14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237 AND SER-310, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310 AND SER-346, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTAF3_YEAST
    AccessioniPrimary (citable) accession number: Q12297
    Secondary accession number(s): D6W401
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 16, 2004
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3