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Q12297 (TAF3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor TFIID subunit 3
Alternative name(s):
TAFII-47
Short name=TAFII47
TBP-associated factor 3
TBP-associated factor 47 kDa
Gene names
Name:TAF3
Synonyms:TAF47
Ordered Locus Names:YPL011C
ORF Names:LPA6C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subunit structure

TAF3 heterodimerizes with TAF10. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Ref.6

Subcellular location

Nucleus Ref.11.

Miscellaneous

Present with 3300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the TAF3 family.

Contains 1 histone-fold domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Transcription initiation factor TFIID subunit 3
PRO_0000118868

Regions

Domain11 – 7868Histone-fold
Coiled coil289 – 30921 Potential

Amino acid modifications

Modified residue2371Phosphothreonine Ref.15
Modified residue3101Phosphoserine Ref.15 Ref.16
Modified residue3461Phosphoserine Ref.16

Sequences

Sequence LengthMass (Da)Tools
Q12297 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 98A6B7813A29978F

FASTA35340,296
        10         20         30         40         50         60 
MTTNNDFYFA LLRISILQLL KAQGFDRARP SLVDVMTDLY AKFLSLLASE VSSIAQARCD 

        70         80         90        100        110        120 
QDDTIALQDI TLALENLGIV KPTNVLDVYD ENSELSSSRG MEKFKDWCIY STQLTDARIT 

       130        140        150        160        170        180 
ALPTVELLQS EEKESDPLSA IPDYLNQLLQ NKGAKQKLET KNRKTELIED LINNNGLDDW 

       190        200        210        220        230        240 
IKLVIARQRI NMIERASKKE SQNVPALPHI AGYKSSILSR HHHTTITNED RMPSAMTPRD 

       250        260        270        280        290        300 
EDALTEIQEN PFVTSKLPIM RKENRLENIT LSFEDEELES LGEVEGPNQK SQENNNEESF 

       310        320        330        340        350 
KENNKSLTES PHGDDRDISM FQFDSNVDTK WAEQEDMDST FQRRTSLDYG GYF 

« Hide

References

« Hide 'large scale' references
[1]"The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M."
Gerring S.L., Spencer F., Hieter P.
EMBO J. 9:4347-4358(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain."
O'Hara P.J., Horowitz H., Eichinger G., Young E.T.
Nucleic Acids Res. 16:10153-10169(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
[6]"Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
Sanders S.L., Weil P.A.
J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[7]"Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
[8]"The histone fold is a key structural motif of transcription factor TFIID."
Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
[9]"Molecular characterization of Saccharomyces cerevisiae TFIID."
Sanders S.L., Garbett K.A., Weil P.A.
Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TFIID STOICHIOMETRY.
[10]"Multi-protein complexes in eukaryotic gene transcription."
Martinez E.
Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]"Mapping histone fold TAFs within yeast TFIID."
Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P.
EMBO J. 21:3424-3433(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[14]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[15]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237 AND SER-310, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310 AND SER-346, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33335 Genomic DNA. Translation: AAB68094.1.
Z71255 Genomic DNA. Translation: CAA95030.1.
Z48483 Genomic DNA. Translation: CAA88375.1.
BK006949 Genomic DNA. Translation: DAA11417.1.
PIRS52520.
RefSeqNP_015314.1. NM_001183825.1.

3D structure databases

ProteinModelPortalQ12297.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36166. 28 interactions.
DIPDIP-5328N.
IntActQ12297. 18 interactions.
MINTMINT-545815.
STRING4932.YPL011C.

Proteomic databases

PaxDbQ12297.
PeptideAtlasQ12297.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL011C; YPL011C; YPL011C.
GeneID856096.
KEGGsce:YPL011C.

Organism-specific databases

CYGDYPL011c.
SGDS000005932. TAF3.

Phylogenomic databases

eggNOGNOG70559.
HOGENOMHOG000141699.
KOK14650.
OMAEAMINNG.
OrthoDBEOG78D7Z5.

Enzyme and pathway databases

BioCycYEAST:G3O-33930-MONOMER.

Gene expression databases

GenevestigatorQ12297.

Family and domain databases

InterProIPR006565. BTP.
[Graphical view]
PfamPF07524. Bromo_TP. 1 hit.
[Graphical view]
SMARTSM00576. BTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981134.

Entry information

Entry nameTAF3_YEAST
AccessionPrimary (citable) accession number: Q12297
Secondary accession number(s): D6W401
Entry history
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families