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Q12297

- TAF3_YEAST

UniProt

Q12297 - TAF3_YEAST

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Protein

Transcription initiation factor TFIID subunit 3

Gene

TAF3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.6 Publications

GO - Molecular functioni

  1. chromatin binding Source: SGD

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. RNA polymerase II transcriptional preinitiation complex assembly Source: SGD
  3. transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-33930-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 3
Alternative name(s):
TAFII-47
Short name:
TAFII47
TBP-associated factor 3
TBP-associated factor 47 kDa
Gene namesi
Name:TAF3
Synonyms:TAF47
Ordered Locus Names:YPL011C
ORF Names:LPA6C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL011c.
SGDiS000005932. TAF3.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. transcription factor TFIID complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Transcription initiation factor TFIID subunit 3PRO_0000118868Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei237 – 2371Phosphothreonine1 Publication
Modified residuei310 – 3101Phosphoserine2 Publications
Modified residuei346 – 3461Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12297.
PaxDbiQ12297.
PeptideAtlasiQ12297.

Expressioni

Gene expression databases

GenevestigatoriQ12297.

Interactioni

Subunit structurei

TAF3 heterodimerizes with TAF10. The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

Protein-protein interaction databases

BioGridi36166. 28 interactions.
DIPiDIP-5328N.
IntActiQ12297. 18 interactions.
MINTiMINT-545815.
STRINGi4932.YPL011C.

Structurei

3D structure databases

ProteinModelPortaliQ12297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7868Histone-foldAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili289 – 30921Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the TAF3 family.Curated
Contains 1 histone-fold domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG70559.
HOGENOMiHOG000141699.
InParanoidiQ12297.
KOiK14650.
OMAiEAMINNG.
OrthoDBiEOG78D7Z5.

Family and domain databases

InterProiIPR006565. BTP.
[Graphical view]
PfamiPF07524. Bromo_TP. 1 hit.
[Graphical view]
SMARTiSM00576. BTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12297-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTNNDFYFA LLRISILQLL KAQGFDRARP SLVDVMTDLY AKFLSLLASE
60 70 80 90 100
VSSIAQARCD QDDTIALQDI TLALENLGIV KPTNVLDVYD ENSELSSSRG
110 120 130 140 150
MEKFKDWCIY STQLTDARIT ALPTVELLQS EEKESDPLSA IPDYLNQLLQ
160 170 180 190 200
NKGAKQKLET KNRKTELIED LINNNGLDDW IKLVIARQRI NMIERASKKE
210 220 230 240 250
SQNVPALPHI AGYKSSILSR HHHTTITNED RMPSAMTPRD EDALTEIQEN
260 270 280 290 300
PFVTSKLPIM RKENRLENIT LSFEDEELES LGEVEGPNQK SQENNNEESF
310 320 330 340 350
KENNKSLTES PHGDDRDISM FQFDSNVDTK WAEQEDMDST FQRRTSLDYG

GYF
Length:353
Mass (Da):40,296
Last modified:November 1, 1996 - v1
Checksum:i98A6B7813A29978F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68094.1.
Z71255 Genomic DNA. Translation: CAA95030.1.
Z48483 Genomic DNA. Translation: CAA88375.1.
BK006949 Genomic DNA. Translation: DAA11417.1.
PIRiS52520.
RefSeqiNP_015314.1. NM_001183825.1.

Genome annotation databases

EnsemblFungiiYPL011C; YPL011C; YPL011C.
GeneIDi856096.
KEGGisce:YPL011C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U33335 Genomic DNA. Translation: AAB68094.1 .
Z71255 Genomic DNA. Translation: CAA95030.1 .
Z48483 Genomic DNA. Translation: CAA88375.1 .
BK006949 Genomic DNA. Translation: DAA11417.1 .
PIRi S52520.
RefSeqi NP_015314.1. NM_001183825.1.

3D structure databases

ProteinModelPortali Q12297.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36166. 28 interactions.
DIPi DIP-5328N.
IntActi Q12297. 18 interactions.
MINTi MINT-545815.
STRINGi 4932.YPL011C.

Proteomic databases

MaxQBi Q12297.
PaxDbi Q12297.
PeptideAtlasi Q12297.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL011C ; YPL011C ; YPL011C .
GeneIDi 856096.
KEGGi sce:YPL011C.

Organism-specific databases

CYGDi YPL011c.
SGDi S000005932. TAF3.

Phylogenomic databases

eggNOGi NOG70559.
HOGENOMi HOG000141699.
InParanoidi Q12297.
KOi K14650.
OMAi EAMINNG.
OrthoDBi EOG78D7Z5.

Enzyme and pathway databases

BioCyci YEAST:G3O-33930-MONOMER.

Miscellaneous databases

NextBioi 981134.

Gene expression databases

Genevestigatori Q12297.

Family and domain databases

InterProi IPR006565. BTP.
[Graphical view ]
Pfami PF07524. Bromo_TP. 1 hit.
[Graphical view ]
SMARTi SM00576. BTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M."
    Gerring S.L., Spencer F., Hieter P.
    EMBO J. 9:4347-4358(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The yeast ADR6 gene encodes homopolymeric amino acid sequences and a potential metal-binding domain."
    O'Hara P.J., Horowitz H., Eichinger G., Young E.T.
    Nucleic Acids Res. 16:10153-10169(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Human TAF(II)28 and TAF(II)18 interact through a histone fold encoded by atypical evolutionary conserved motifs also found in the SPT3 family."
    Birck C., Poch O., Romier C., Ruff M., Mengus G., Lavigne A.C., Davidson I., Moras D.
    Cell 94:239-249(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TAF-TAF INTERACTION THROUGH HISTONE-FOLD DOMAIN.
  6. "Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex."
    Sanders S.L., Weil P.A.
    J. Biol. Chem. 275:13895-13900(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  7. "Histone folds mediate selective heterodimerization of yeast TAF(II)25 with TFIID components yTAF(II)47 and yTAF(II)65 and with SAGA component ySPT7."
    Gangloff Y.G., Sanders S.L., Romier C., Kirschner D.B., Weil P.A., Tora L., Davidson I.
    Mol. Cell. Biol. 21:1841-1853(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION IN TFIID AND SAGA.
  8. "The histone fold is a key structural motif of transcription factor TFIID."
    Gangloff Y.G., Romier C., Thuault S., Werten S., Davidson I.
    Trends Biochem. Sci. 26:250-257(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, HISTONE-FOLD DOMAIN CHARACTERIZATION.
  9. "Molecular characterization of Saccharomyces cerevisiae TFIID."
    Sanders S.L., Garbett K.A., Weil P.A.
    Mol. Cell. Biol. 22:6000-6013(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TFIID STOICHIOMETRY.
  10. "Multi-protein complexes in eukaryotic gene transcription."
    Martinez E.
    Plant Mol. Biol. 50:925-947(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  13. Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-237 AND SER-310, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-310 AND SER-346, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTAF3_YEAST
AccessioniPrimary (citable) accession number: Q12297
Secondary accession number(s): D6W401
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3