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Protein

Protein MAM3

Gene

MAM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in metal homeostasis and more specially in manganese sensitivity.1 Publication

GO - Biological processi

  • cellular manganese ion homeostasis Source: SGD
  • mitochondrion organization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33469-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MAM3
Gene namesi
Name:MAM3
Ordered Locus Names:YOL060C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL060C.
SGDiS000005421. MAM3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616VacuolarSequence analysisAdd
BLAST
Transmembranei17 – 3721HelicalSequence analysisAdd
BLAST
Topological domaini38 – 6528CytoplasmicSequence analysisAdd
BLAST
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Topological domaini87 – 12034VacuolarSequence analysisAdd
BLAST
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 1454CytoplasmicSequence analysis
Transmembranei146 – 16621HelicalSequence analysisAdd
BLAST
Topological domaini167 – 17711VacuolarSequence analysisAdd
BLAST
Transmembranei178 – 19821HelicalSequence analysisAdd
BLAST
Topological domaini199 – 706508CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 706706Protein MAM3PRO_0000255960Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei439 – 4391PhosphoserineCombined sources
Modified residuei447 – 4471PhosphoserineCombined sources
Modified residuei527 – 5271PhosphoserineCombined sources
Modified residuei603 – 6031PhosphoserineCombined sources
Modified residuei604 – 6041PhosphotyrosineCombined sources
Modified residuei607 – 6071PhosphothreonineCombined sources
Modified residuei614 – 6141PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12296.
TopDownProteomicsiQ12296.

PTM databases

iPTMnetiQ12296.

Interactioni

Protein-protein interaction databases

BioGridi34341. 16 interactions.
IntActiQ12296. 25 interactions.
MINTiMINT-4501772.

Structurei

3D structure databases

ProteinModelPortaliQ12296.
SMRiQ12296. Positions 239-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 233169DUF21Add
BLAST
Domaini259 – 32062CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini321 – 38666CBS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ACDP family.Curated
Contains 2 CBS domains.PROSITE-ProRule annotation
Contains 1 DUF21 domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000002383.
HOGENOMiHOG000183089.
InParanoidiQ12296.
KOiK16302.
OMAiKRHITSY.
OrthoDBiEOG74N5RB.

Family and domain databases

InterProiIPR000644. CBS_dom.
IPR002550. DUF21.
[Graphical view]
PfamiPF01595. DUF21. 1 hit.
[Graphical view]
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFLPLRSRS RSGAPHWVYI ILYHIFTIPK IYSLPLLSGS HVLNSRDVAD
60 70 80 90 100
SGHSVGDEAS VTTYYIISII LVLLGGVFAG LTLGLMGQDE VYLKVISTSG
110 120 130 140 150
SNSEKKLAKR VLDLISRGKH WVLVTLLLSN VITNETLPIV LDRCLGGGWQ
160 170 180 190 200
AVVSSTILIV IFGEIIPQSV CVKYGLQVGA FFCPFVLVLM YLMYPVAYPI
210 220 230 240 250
ATLLDYMLGE DHGTMYKKSG LKTLVTLHRT MGVERLTKDE VTIISAVLDL
260 270 280 290 300
KAKRVEEIMT PIENVFTMSA DTILDDKTVE KIFNSGFSRI PIFLPNEPNN
310 320 330 340 350
FIGMLLVRVL ISYDPDDCLP ISHFPLATLP ETSPNTSCLN ILNYFQEGKA
360 370 380 390 400
HMCVVSKEPG SSHGAIGVLT LEDVIEELIG EEIVDESDVF VDMHQHIMRQ
410 420 430 440 450
QPGPLSKRHI TSYLHHLYTS SHKEHKAADQ ADESSPLLSP SNSNHPSEHP
460 470 480 490 500
QQDLNNKSWK QKSNDGYDRS NAVLSPTPQV TEHGTIIPSN LASNPLNVNK
510 520 530 540 550
SFVTIKKPAN VPKIITTHTP HSSKEPSPAP HSNDKSLSAE EQQLLSDHAE
560 570 580 590 600
LSRQAVLHTQ RSGQPTQVTT STKTTRNSPD SISIPNSGAN HGNENQNVTI
610 620 630 640 650
STSYQNTKNG IVESVITVKG VPKTIIGPAK DWDESKSEYG NENINQENSN
660 670 680 690 700
RSDDRESSSS NASLFSSIKN KFKNENANNN DRSNFTDSLS RTSNYDANGS

SSTIKR
Length:706
Mass (Da):77,712
Last modified:November 1, 1996 - v1
Checksum:iAAB3EA4CAD8E5F53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91067 Genomic DNA. Translation: CAA62524.1.
Z74802 Genomic DNA. Translation: CAA99069.1.
AY692822 Genomic DNA. Translation: AAT92841.1.
BK006948 Genomic DNA. Translation: DAA10723.1.
PIRiS61717.
RefSeqiNP_014581.1. NM_001183315.1.

Genome annotation databases

EnsemblFungiiYOL060C; YOL060C; YOL060C.
GeneIDi854094.
KEGGisce:YOL060C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91067 Genomic DNA. Translation: CAA62524.1.
Z74802 Genomic DNA. Translation: CAA99069.1.
AY692822 Genomic DNA. Translation: AAT92841.1.
BK006948 Genomic DNA. Translation: DAA10723.1.
PIRiS61717.
RefSeqiNP_014581.1. NM_001183315.1.

3D structure databases

ProteinModelPortaliQ12296.
SMRiQ12296. Positions 239-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34341. 16 interactions.
IntActiQ12296. 25 interactions.
MINTiMINT-4501772.

PTM databases

iPTMnetiQ12296.

Proteomic databases

MaxQBiQ12296.
TopDownProteomicsiQ12296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL060C; YOL060C; YOL060C.
GeneIDi854094.
KEGGisce:YOL060C.

Organism-specific databases

EuPathDBiFungiDB:YOL060C.
SGDiS000005421. MAM3.

Phylogenomic databases

GeneTreeiENSGT00390000002383.
HOGENOMiHOG000183089.
InParanoidiQ12296.
KOiK16302.
OMAiKRHITSY.
OrthoDBiEOG74N5RB.

Enzyme and pathway databases

BioCyciYEAST:G3O-33469-MONOMER.

Miscellaneous databases

NextBioi975755.
PROiQ12296.

Family and domain databases

InterProiIPR000644. CBS_dom.
IPR002550. DUF21.
[Graphical view]
PfamiPF01595. DUF21. 1 hit.
[Graphical view]
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 26 kb region on the left arm of yeast chromosome XV."
    Mannhaupt G., Vetter I., Schwarzlose C., Mitzel S., Feldmann H.
    Yeast 12:67-76(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Manganese toxicity and Saccharomyces cerevisiae Mam3p, a member of the ACDP (ancient conserved domain protein) family."
    Yang M., Jensen L.T., Gardner A.J., Culotta V.C.
    Biochem. J. 386:479-487(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-614, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-439; SER-447; SER-603; TYR-604 AND THR-607, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMAM3_YEAST
AccessioniPrimary (citable) accession number: Q12296
Secondary accession number(s): D6W207
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2270 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.