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Protein

Autophagy-related protein 34

Gene

ATG34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cargo-receptor protein involved in the cytoplasm to vacuole transport (Cvt) and in autophagy. Recognizes cargo proteins, such as AMS1 and delivers them to the pre-autophagosomal structure for eventual engulfment by the autophagosome and targeting to the vacuole.2 Publications

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • protein complex assembly Source: SGD
  • protein complex localization Source: SGD
  • protein targeting to vacuole involved in autophagy Source: SGD
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33486-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 34
Gene namesi
Name:ATG34
Synonyms:ATG19-B
Ordered Locus Names:YOL083W
ORF Names:O0957
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL083W.
SGDiS000005443. ATG34.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Autophagy-related protein 34PRO_0000235927Add
BLAST

Proteomic databases

MaxQBiQ12292.
PeptideAtlasiQ12292.

PTM databases

iPTMnetiQ12292.

Interactioni

Subunit structurei

Interacts with AMS1, ATG8 and ATG11.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AMS1P228553EBI-36362,EBI-10398
HRR25P292952EBI-36362,EBI-8536

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi34319. 21 interactions.
DIPiDIP-1598N.
IntActiQ12292. 17 interactions.
MINTiMINT-389176.

Structurei

Secondary structure

1
412
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi251 – 2544Combined sources
Beta strandi263 – 2664Combined sources
Beta strandi269 – 2713Combined sources
Beta strandi278 – 2814Combined sources
Beta strandi284 – 2863Combined sources
Beta strandi303 – 3053Combined sources
Beta strandi322 – 3243Combined sources
Beta strandi330 – 3334Combined sources
Beta strandi338 – 3403Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KZKNMR-A246-348[»]
ProteinModelPortaliQ12292.
SMRiQ12292. Positions 246-348.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12292.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni246 – 348103AMS1-bindingAdd
BLAST

Phylogenomic databases

GeneTreeiENSGT00530000068761.
InParanoidiQ12292.
OrthoDBiEOG7WX0JR.

Family and domain databases

InterProiIPR024543. Atg19/Atg34_C.
[Graphical view]
PfamiPF12744. ATG19_autophagy. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12292-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIAVETTLF DFFVIDQFKK STFSAPNTKV DTIKGCINKF IEQFNVYDEQ
60 70 80 90 100
HIFWQPPGKS NVRLLSNAND FGQLGNFLHK KIKCNIFIGE EALRKYDLNI
110 120 130 140 150
CGPYDKFVEN SDPSVKKVVN RDDVMLSRKC LNIISEQLSI LEKSISKAQN
160 170 180 190 200
QVLQSSEVEG KKCIILPEDK PELIKFFSKF ETSVQLQEVY EGYKVYEKLL
210 220 230 240 250
QKFGGQKKRM ESFLNENTPM SGAEAIKQIN ISEELKEKGE RLTTPNDPLL
260 270 280 290 300
HVEVSNEDNS LHFILYNKTN IIIPGNCTFE FSSQISEVFS IKMGPHEIGI
310 320 330 340 350
KGQKELWFFP SLPTPLSNYT MKVVNQDGET ILVGKCADSN EITLKSPLAS
360 370 380 390 400
FSTGSFQTGS FHTLQDPTNV FRADALSSPD ESSIMSTPFL GETDEVYNSG
410
STLSRPFTWE EI
Length:412
Mass (Da):46,655
Last modified:November 1, 1996 - v1
Checksum:i5F339CC6AC2824D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83121 Genomic DNA. Translation: CAA58197.1.
Z74825 Genomic DNA. Translation: CAA99095.1.
BK006948 Genomic DNA. Translation: DAA10701.1.
PIRiS57387.
RefSeqiNP_014558.1. NM_001183337.1.

Genome annotation databases

EnsemblFungiiYOL083W; YOL083W; YOL083W.
GeneIDi854071.
KEGGisce:YOL083W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83121 Genomic DNA. Translation: CAA58197.1.
Z74825 Genomic DNA. Translation: CAA99095.1.
BK006948 Genomic DNA. Translation: DAA10701.1.
PIRiS57387.
RefSeqiNP_014558.1. NM_001183337.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KZKNMR-A246-348[»]
ProteinModelPortaliQ12292.
SMRiQ12292. Positions 246-348.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34319. 21 interactions.
DIPiDIP-1598N.
IntActiQ12292. 17 interactions.
MINTiMINT-389176.

PTM databases

iPTMnetiQ12292.

Proteomic databases

MaxQBiQ12292.
PeptideAtlasiQ12292.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL083W; YOL083W; YOL083W.
GeneIDi854071.
KEGGisce:YOL083W.

Organism-specific databases

EuPathDBiFungiDB:YOL083W.
SGDiS000005443. ATG34.

Phylogenomic databases

GeneTreeiENSGT00530000068761.
InParanoidiQ12292.
OrthoDBiEOG7WX0JR.

Enzyme and pathway databases

BioCyciYEAST:G3O-33486-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12292.
PROiQ12292.

Family and domain databases

InterProiIPR024543. Atg19/Atg34_C.
[Graphical view]
PfamiPF12744. ATG19_autophagy. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 29.425 kb segment on the left arm of yeast chromosome XV contains more than twice as many unknown as known open reading frames."
    Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.
    Yeast 11:975-986(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "Selective transport of alpha-mannosidase by autophagic pathways: identification of a novel receptor, Atg34p."
    Suzuki K., Kondo C., Morimoto M., Ohsumi Y.
    J. Biol. Chem. 285:30019-30025(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH AMS1; ATG8 AND ATG11.
  6. "Selective transport of alpha-mannosidase by autophagic pathways: structural basis for cargo recognition by Atg19 and Atg34."
    Watanabe Y., Noda N.N., Kumeta H., Suzuki K., Ohsumi Y., Inagaki F.
    J. Biol. Chem. 285:30026-30033(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 246-348, FUNCTION, DOMAIN AMS1-BINDING, INTERACTION WITH AMS1.

Entry informationi

Entry nameiATG34_YEAST
AccessioniPrimary (citable) accession number: Q12292
Secondary accession number(s): D6W1Y5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.