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Protein

25S rRNA (uridine(2843)-N(3))-methyltransferase

Gene

BMT6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N3 position of uridine 2843 (m3U2843) in 25S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + uracil(2843) in 25S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(2843) in 25S rRNA.1 Publication

GO - Molecular functioni

  • rRNA (uridine-N3-)-methyltransferase activity Source: SGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: SGD

GO - Biological processi

  • rRNA base methylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciYEAST:G3O-32216-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
25S rRNA (uridine(2843)-N(3))-methyltransferase (EC:2.1.1.3121 Publication)
Alternative name(s):
Base methyltransferase of 25S RNA 6
Gene namesi
Name:BMT6
Ordered Locus Names:YLR063W
ORF Names:L2174
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR063W.
SGDiS000004053. BMT6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi294 – 2941G → R: Abolishes methyltransferase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 36536525S rRNA (uridine(2843)-N(3))-methyltransferasePRO_0000247230Add
BLAST

Proteomic databases

MaxQBiQ12291.

Interactioni

Protein-protein interaction databases

BioGridi31337. 21 interactions.
DIPiDIP-4828N.
IntActiQ12291. 1 interaction.
MINTiMINT-474205.

Structurei

3D structure databases

ProteinModelPortaliQ12291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000001109.
InParanoidiQ12291.
KOiK19308.
OMAiLYEREFA.
OrthoDBiEOG71CFWV.

Family and domain databases

InterProiIPR021463. Methyltransf_34.
[Graphical view]
PfamiPF11312. Methyltransf_34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLMRRFAFL TSSVYFKYIP IYSQYHYSSQ FPINMNPKKV AQLPVHNKST
60 70 80 90 100
LPPQEIIDLF KITFLEELYP KDQDNEKSPL TEQIQAVKSD LYNRDYNAAF
110 120 130 140 150
NNDSKRIAYC CRWSPSRATS YASVFAHFPE LLKIIRCEID DKDSNVLCIG
160 170 180 190 200
GGAGGELVAL ASIFTLSRDF SSKFASALKI DNEVNKKPRN LNIQLVDIAD
210 220 230 240 250
WSTVVEKLTA TIKSKWLYGD SEAESFNVNF THKDCLQMTE PQDIKIYQGL
260 270 280 290 300
DLITLLFTTN ELFTQKKVES IKFLQRLNEN CAPGCHLLIL ESAGSYSHIT
310 320 330 340 350
INNKKFPIQF LIDTILVGNR KDKGTTGPWS LVSENDSIWY RMDPKLDYSI
360
PLENMRFFYR LYVKN
Length:365
Mass (Da):42,041
Last modified:November 1, 1996 - v1
Checksum:iA072FB00FD3A110C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64309.1.
Z73235 Genomic DNA. Translation: CAA97619.1.
BK006945 Genomic DNA. Translation: DAA09380.1.
PIRiS61636.
RefSeqiNP_013164.1. NM_001181950.1.

Genome annotation databases

EnsemblFungiiYLR063W; YLR063W; YLR063W.
GeneIDi850752.
KEGGisce:YLR063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94607 Genomic DNA. Translation: CAA64309.1.
Z73235 Genomic DNA. Translation: CAA97619.1.
BK006945 Genomic DNA. Translation: DAA09380.1.
PIRiS61636.
RefSeqiNP_013164.1. NM_001181950.1.

3D structure databases

ProteinModelPortaliQ12291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31337. 21 interactions.
DIPiDIP-4828N.
IntActiQ12291. 1 interaction.
MINTiMINT-474205.

Proteomic databases

MaxQBiQ12291.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR063W; YLR063W; YLR063W.
GeneIDi850752.
KEGGisce:YLR063W.

Organism-specific databases

EuPathDBiFungiDB:YLR063W.
SGDiS000004053. BMT6.

Phylogenomic databases

HOGENOMiHOG000001109.
InParanoidiQ12291.
KOiK19308.
OMAiLYEREFA.
OrthoDBiEOG71CFWV.

Enzyme and pathway databases

BioCyciYEAST:G3O-32216-MONOMER.

Miscellaneous databases

PROiQ12291.

Family and domain databases

InterProiIPR021463. Methyltransf_34.
[Graphical view]
PfamiPF11312. Methyltransf_34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. "Identification of novel methyltransferases, Bmt5 and Bmt6, responsible for the m3U methylations of 25S rRNA in Saccharomyces cerevisiae."
    Sharma S., Yang J., Duttmann S., Watzinger P., Kotter P., Entian K.D.
    Nucleic Acids Res. 42:3246-3260(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, MUTAGENESIS OF GLY-294.

Entry informationi

Entry nameiBMT6_YEAST
AccessioniPrimary (citable) accession number: Q12291
Secondary accession number(s): D6VY64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.