ID   MDY2_YEAST              Reviewed;         212 AA.
AC   Q12285; D6W1V6;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 170.
DE   RecName: Full=Ubiquitin-like protein MDY2;
DE   AltName: Full=Golgi to ER traffic protein 5;
DE   AltName: Full=Mating-deficient protein 2;
DE   AltName: Full=Translation machinery-associated protein 24;
GN   Name=MDY2; Synonyms=GET5, TMA24; OrderedLocusNames=YOL111C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=7502582; DOI=10.1002/yea.320111108;
RA   Vandenbol M., Durand P., Portetelle D., Hilger F.;
RT   "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including
RT   the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for
RT   tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element.";
RL   Yeast 11:1069-1075(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169874;
RA   Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA   Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA   Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA   Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA   Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA   Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA   Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA   Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA   Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA   Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA   Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA   Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA   Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA   Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA   Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA   Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL   Nature 387:98-102(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=10514570;
RX   DOI=10.1002/(sici)1097-0061(199910)15:14<1529::aid-yea457>3.0.co;2-y;
RA   Iwanejko L., Smith K.N., Loeillet S., Nicolas A., Fabre F.;
RT   "Disruption and functional analysis of six ORFs on chromosome XV: YOL117w,
RT   YOL115w (TRF4), YOL114c, YOL112w (MSB4), YOL111c and YOL072w.";
RL   Yeast 15:1529-1539(1999).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=16390866; DOI=10.1242/jcs.02754;
RA   Hu Z., Potthoff B., Hollenberg C.P., Ramezani-Rad M.;
RT   "Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient
RT   mating in Saccharomyces cerevisiae.";
RL   J. Cell Sci. 119:326-338(2006).
RN   [8]
RP   X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 1-59 IN COMPLEX WITH GET4, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=20106980; DOI=10.1074/jbc.m109.087098;
RA   Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D.,
RA   Wang C.;
RT   "Crystal structure of Get4-Get5 complex and its interactions with Sgt2,
RT   Get3, and Ydj1.";
RL   J. Biol. Chem. 285:9962-9970(2010).
CC   -!- FUNCTION: Required for efficient mating. Involved in the production of
CC       alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear
CC       migration into pheromone-induced shmoos. {ECO:0000269|PubMed:10514570,
CC       ECO:0000269|PubMed:16390866}.
CC   -!- SUBUNIT: Interacts with GET4. {ECO:0000269|PubMed:20106980}.
CC   -!- INTERACTION:
CC       Q12285; Q12154: GET3; NbExp=10; IntAct=EBI-34904, EBI-2989;
CC       Q12285; Q12125: GET4; NbExp=17; IntAct=EBI-34904, EBI-36940;
CC       Q12285; P31539: HSP104; NbExp=2; IntAct=EBI-34904, EBI-8050;
CC       Q12285; P22214: SEC22; NbExp=3; IntAct=EBI-34904, EBI-16577;
CC       Q12285; Q12118: SGT2; NbExp=15; IntAct=EBI-34904, EBI-31784;
CC       Q12285; P25491: YDJ1; NbExp=2; IntAct=EBI-34904, EBI-10420;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol. Nucleus. Note=Detected in
CC       both cytosol and nucleus but is predominantly cytosolic.
CC   -!- MISCELLANEOUS: Present with 6510 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z48149; CAA88151.1; -; Genomic_DNA.
DR   EMBL; Z74853; CAA99130.1; -; Genomic_DNA.
DR   EMBL; BK006948; DAA10672.1; -; Genomic_DNA.
DR   PIR; S51888; S51888.
DR   RefSeq; NP_014530.1; NM_001183365.1.
DR   PDB; 2LNZ; NMR; -; A/B=152-212.
DR   PDB; 2LXA; NMR; -; A=74-151.
DR   PDB; 2LXC; NMR; -; A=74-151.
DR   PDB; 2WPV; X-ray; 1.99 A; B/D/F/H=1-59.
DR   PDB; 3LKU; X-ray; 2.80 A; B/D/F=3-56.
DR   PDB; 3VEJ; X-ray; 1.23 A; A/B=175-212.
DR   PDB; 3ZDM; X-ray; 1.80 A; C/F=71-151.
DR   PDB; 4A20; X-ray; 1.78 A; A=70-152.
DR   PDB; 4ASW; NMR; -; C=70-152.
DR   PDB; 4GOC; X-ray; 2.40 A; A/B/C=74-148.
DR   PDB; 4PWX; X-ray; 5.40 A; D/F=1-54.
DR   PDB; 5BW8; X-ray; 2.80 A; D=1-54.
DR   PDB; 5BWK; X-ray; 6.00 A; F/H/J/L/R/T/V/X=1-56.
DR   PDBsum; 2LNZ; -.
DR   PDBsum; 2LXA; -.
DR   PDBsum; 2LXC; -.
DR   PDBsum; 2WPV; -.
DR   PDBsum; 3LKU; -.
DR   PDBsum; 3VEJ; -.
DR   PDBsum; 3ZDM; -.
DR   PDBsum; 4A20; -.
DR   PDBsum; 4ASW; -.
DR   PDBsum; 4GOC; -.
DR   PDBsum; 4PWX; -.
DR   PDBsum; 5BW8; -.
DR   PDBsum; 5BWK; -.
DR   AlphaFoldDB; Q12285; -.
DR   BMRB; Q12285; -.
DR   SMR; Q12285; -.
DR   BioGRID; 34289; 350.
DR   ComplexPortal; CPX-1861; GET4-GET5 transmembrane domain recognition complex.
DR   DIP; DIP-1982N; -.
DR   IntAct; Q12285; 13.
DR   MINT; Q12285; -.
DR   STRING; 4932.YOL111C; -.
DR   TCDB; 3.A.21.1.1; the c-terminal tail-anchored membrane protein biogenesis/ insertion complex (tamp-b) family.
DR   MaxQB; Q12285; -.
DR   PaxDb; 4932-YOL111C; -.
DR   PeptideAtlas; Q12285; -.
DR   EnsemblFungi; YOL111C_mRNA; YOL111C; YOL111C.
DR   GeneID; 854038; -.
DR   KEGG; sce:YOL111C; -.
DR   AGR; SGD:S000005471; -.
DR   SGD; S000005471; MDY2.
DR   VEuPathDB; FungiDB:YOL111C; -.
DR   eggNOG; KOG0011; Eukaryota.
DR   HOGENOM; CLU_1294717_0_0_1; -.
DR   InParanoid; Q12285; -.
DR   OMA; NCMVSAP; -.
DR   OrthoDB; 2019738at2759; -.
DR   BioCyc; YEAST:G3O-33508-MONOMER; -.
DR   BioGRID-ORCS; 854038; 0 hits in 10 CRISPR screens.
DR   ChiTaRS; MDY2; yeast.
DR   EvolutionaryTrace; Q12285; -.
DR   PRO; PR:Q12285; -.
DR   Proteomes; UP000002311; Chromosome XV.
DR   RNAct; Q12285; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0072380; C:TRC complex; IDA:SGD.
DR   GO; GO:0000753; P:cell morphogenesis involved in conjugation with cellular fusion; IMP:SGD.
DR   GO; GO:0006620; P:post-translational protein targeting to endoplasmic reticulum membrane; IDA:SGD.
DR   GO; GO:0045048; P:protein insertion into ER membrane; HGI:SGD.
DR   CDD; cd01805; Ubl_Rad23; 1.
DR   Gene3D; 1.10.286.70; Get5 dimerization domain; 1.
DR   Gene3D; 1.20.5.510; Single helix bin; 1.
DR   InterPro; IPR040474; Get5_C.
DR   InterPro; IPR031765; Mdy2_get4-bd.
DR   InterPro; IPR000626; Ubiquitin-like_dom.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR46555; UBIQUITIN-LIKE PROTEIN 4A; 1.
DR   PANTHER; PTHR46555:SF1; UBIQUITIN-LIKE PROTEIN 4A; 1.
DR   Pfam; PF16843; Get5_bdg; 1.
DR   Pfam; PF18514; Get5_C; 1.
DR   Pfam; PF00240; ubiquitin; 1.
DR   SMART; SM00213; UBQ; 1.
DR   SUPFAM; SSF54236; Ubiquitin-like; 1.
DR   PROSITE; PS50053; UBIQUITIN_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Nucleus; Reference proteome.
FT   CHAIN           1..212
FT                   /note="Ubiquitin-like protein MDY2"
FT                   /id="PRO_0000235917"
FT   DOMAIN          74..152
FT                   /note="Ubiquitin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT   REGION          150..177
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           4..6
FT                   /evidence="ECO:0007829|PDB:3LKU"
FT   HELIX           8..20
FT                   /evidence="ECO:0007829|PDB:2WPV"
FT   HELIX           35..37
FT                   /evidence="ECO:0007829|PDB:2WPV"
FT   STRAND          74..80
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   STRAND          82..84
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   STRAND          86..92
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   HELIX           98..107
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   HELIX           114..116
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   STRAND          117..121
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   STRAND          124..126
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   HELIX           132..134
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   STRAND          139..141
FT                   /evidence="ECO:0007829|PDB:3ZDM"
FT   STRAND          143..148
FT                   /evidence="ECO:0007829|PDB:4A20"
FT   HELIX           179..189
FT                   /evidence="ECO:0007829|PDB:3VEJ"
FT   TURN            190..192
FT                   /evidence="ECO:0007829|PDB:3VEJ"
FT   HELIX           194..210
FT                   /evidence="ECO:0007829|PDB:3VEJ"
SQ   SEQUENCE   212 AA;  23732 MW;  CE6FC4282E0E472C CRC64;
     MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY KYKQNRAKKL
     KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ IKQHLISEEK ASHISEIKLL
     LKGKVLHDNL FLSDLKVTPA NSTITVMIKP NPTISKEPEA EKSTNSPAPA PPQELTVPWD
     DIEALLKNNF ENDQAAVRQV MERLQKGWSL AK
//