Ubiquitin-like protein MDY2 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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Q12285 (MDY2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ubiquitin-like protein MDY2

Alternative name(s):
Golgi to ER traffic protein 5
Mating-deficient protein 2
Translation machinery-associated protein 24

Gene names

Name:	MDY2
Synonyms:	GET5, TMA24
Ordered Locus Names:	YOL111C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos. Ref.4 Ref.7
Subunit structure	Interacts with GET4.
Subcellular location	Cytoplasm › cytosol. Nucleus. Note: Detected in both cytosol and nucleus but is predominantly cytosolic. Ref.5 Ref.7 Ref.8
Miscellaneous	Present with 6510 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell morphogenesis involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.7. Source: SGD posttranslational protein targeting to membrane Inferred from direct assay PubMed 20850366. Source: SGD protein insertion into ER membrane Inferred from genetic interaction PubMed 19325107. Source: SGD
Cellular_component	TRC complex Inferred from direct assay PubMed 20850366. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
CIA1	Q05583	3	EBI-34904,EBI-32145
GET3	Q12154	3	EBI-34904,EBI-2989
GET4	Q12125	8	EBI-34904,EBI-36940
SGT2	Q12118	9	EBI-34904,EBI-31784
YDJ1	P25491	2	EBI-34904,EBI-10420

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 212

212

Ubiquitin-like protein MDY2

PRO_0000235917

Regions

Domain

74 – 152

Ubiquitin-like

Secondary structure

212

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q12285 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: CE6FC4282E0E472C
Show »

FASTA

212

23,732

        10         20         30         40         50         60 
MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY KYKQNRAKKL 

        70         80         90        100        110        120 
KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ IKQHLISEEK ASHISEIKLL 

       130        140        150        160        170        180 
LKGKVLHDNL FLSDLKVTPA NSTITVMIKP NPTISKEPEA EKSTNSPAPA PPQELTVPWD 

       190        200        210 
DIEALLKNNF ENDQAAVRQV MERLQKGWSL AK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element." Vandenbol M., Durand P., Portetelle D., Hilger F. Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w (TRF4), YOL114c, YOL112w (MSB4), YOL111c and YOL072w." Iwanejko L., Smith K.N., Loeillet S., Nicolas A., Fabre F. Yeast 15:1529-1539(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae." Hu Z., Potthoff B., Hollenberg C.P., Ramezani-Rad M. J. Cell Sci. 119:326-338(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1." Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C. J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 1-59 IN COMPLEX WITH GET4, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z48149 Genomic DNA. Translation: CAA88151.1.
Z74853 Genomic DNA. Translation: CAA99130.1.
BK006948 Genomic DNA. Translation: DAA10672.1.

PIR

S51888.

RefSeq

NP_014530.1. NM_001183365.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2LNZ	NMR	-	A/B	152-212	[»]
2LXA	NMR	-	A	74-151	[»]
2LXC	NMR	-	A	74-151	[»]
2WPV	X-ray	1.99	B/D/F/H	1-59	[»]
3LKU	X-ray	2.80	B/D/F	3-56	[»]
3VEJ	X-ray	1.23	A/B	175-212	[»]
4A20	X-ray	1.78	A	70-152	[»]
4ASW	NMR	-	C	70-152	[»]
4GOC	X-ray	2.40	A/B/C	74-148	[»]

ProteinModelPortal

Q12285.

SMR

Q12285. Positions 3-56, 72-151, 173-212.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1982N.

IntAct

Q12285. 8 interactions.

MINT

MINT-411662.

STRING

4932.YOL111C.

Proteomic databases

PaxDb

Q12285.

PeptideAtlas

Q12285.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YOL111C; YOL111C; YOL111C.

GeneID

854038.

KEGG

sce:YOL111C.

Organism-specific databases

CYGD

YOL111c.

SGD

S000005471. MDY2.

Phylogenomic databases

eggNOG

NOG138422.

HOGENOM

HOG000113515.

OMA

GKVLHDN.

OrthoDB

EOG41G6DT.

Enzyme and pathway databases

BioCyc

YEAST:G3O-33508-MONOMER.

Gene expression databases

Genevestigator

Q12285.

GermOnline

YOL111C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000626. Ubiquitin.
IPR019955. Ubiquitin_supergroup.
[Graphical view]

Pfam

PF00240. ubiquitin. 1 hit.
[Graphical view]

SMART

SM00213. UBQ. 1 hit.
[Graphical view]

PROSITE

PS00299. UBIQUITIN_1. False negative.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q12285.

NextBio

975601.

Entry information

Entry name

MDY2_YEAST

Accession

Primary (citable) accession number: Q12285
Secondary accession number(s): D6W1V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents