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Protein

Ubiquitin-like protein MDY2

Gene

MDY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.2 Publications

GO - Biological processi

  1. cell morphogenesis involved in conjugation with cellular fusion Source: SGD
  2. posttranslational protein targeting to membrane Source: SGD
  3. protein insertion into ER membrane Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33508-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein MDY2
Alternative name(s):
Golgi to ER traffic protein 5
Mating-deficient protein 2
Translation machinery-associated protein 24
Gene namesi
Name:MDY2
Synonyms:GET5, TMA24
Ordered Locus Names:YOL111C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOL111c.
EuPathDBiFungiDB:YOL111C.
SGDiS000005471. MDY2.

Subcellular locationi

  1. Cytoplasmcytosol
  2. Nucleus

  3. Note: Detected in both cytosol and nucleus but is predominantly cytosolic.

GO - Cellular componenti

  1. nucleus Source: SGD
  2. TRC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Ubiquitin-like protein MDY2PRO_0000235917Add
BLAST

Proteomic databases

MaxQBiQ12285.
PaxDbiQ12285.
PeptideAtlasiQ12285.

Expressioni

Gene expression databases

GenevestigatoriQ12285.

Interactioni

Subunit structurei

Interacts with GET4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121543EBI-34904,EBI-2989
GET4Q121258EBI-34904,EBI-36940
SGT2Q121189EBI-34904,EBI-31784
YDJ1P254912EBI-34904,EBI-10420

Protein-protein interaction databases

BioGridi34289. 83 interactions.
DIPiDIP-1982N.
IntActiQ12285. 9 interactions.
MINTiMINT-411662.
STRINGi4932.YOL111C.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Helixi8 – 2013Combined sources
Helixi35 – 373Combined sources
Beta strandi74 – 807Combined sources
Beta strandi82 – 843Combined sources
Beta strandi86 – 927Combined sources
Helixi98 – 10710Combined sources
Helixi114 – 1163Combined sources
Beta strandi117 – 1215Combined sources
Beta strandi124 – 1263Combined sources
Helixi132 – 1343Combined sources
Beta strandi139 – 1413Combined sources
Beta strandi143 – 1486Combined sources
Helixi179 – 18911Combined sources
Turni190 – 1923Combined sources
Helixi194 – 21017Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LNZNMR-A/B152-212[»]
2LXANMR-A74-151[»]
2LXCNMR-A74-151[»]
2WPVX-ray1.99B/D/F/H1-59[»]
3LKUX-ray2.80B/D/F3-56[»]
3VEJX-ray1.23A/B175-212[»]
3ZDMX-ray1.80C/F71-151[»]
4A20X-ray1.78A70-152[»]
4ASWNMR-C70-152[»]
4GOCX-ray2.40A/B/C74-148[»]
4PWXX-ray5.40D/F1-54[»]
ProteinModelPortaliQ12285.
SMRiQ12285. Positions 3-56, 72-151, 173-212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12285.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 15279Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG138422.
HOGENOMiHOG000113515.
InParanoidiQ12285.
OMAiGKVLHDN.
OrthoDBiEOG7XH723.

Family and domain databases

InterProiIPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY
60 70 80 90 100
KYKQNRAKKL KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ
110 120 130 140 150
IKQHLISEEK ASHISEIKLL LKGKVLHDNL FLSDLKVTPA NSTITVMIKP
160 170 180 190 200
NPTISKEPEA EKSTNSPAPA PPQELTVPWD DIEALLKNNF ENDQAAVRQV
210
MERLQKGWSL AK
Length:212
Mass (Da):23,732
Last modified:November 1, 1996 - v1
Checksum:iCE6FC4282E0E472C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88151.1.
Z74853 Genomic DNA. Translation: CAA99130.1.
BK006948 Genomic DNA. Translation: DAA10672.1.
PIRiS51888.
RefSeqiNP_014530.1. NM_001183365.1.

Genome annotation databases

EnsemblFungiiYOL111C; YOL111C; YOL111C.
GeneIDi854038.
KEGGisce:YOL111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88151.1.
Z74853 Genomic DNA. Translation: CAA99130.1.
BK006948 Genomic DNA. Translation: DAA10672.1.
PIRiS51888.
RefSeqiNP_014530.1. NM_001183365.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LNZNMR-A/B152-212[»]
2LXANMR-A74-151[»]
2LXCNMR-A74-151[»]
2WPVX-ray1.99B/D/F/H1-59[»]
3LKUX-ray2.80B/D/F3-56[»]
3VEJX-ray1.23A/B175-212[»]
3ZDMX-ray1.80C/F71-151[»]
4A20X-ray1.78A70-152[»]
4ASWNMR-C70-152[»]
4GOCX-ray2.40A/B/C74-148[»]
4PWXX-ray5.40D/F1-54[»]
ProteinModelPortaliQ12285.
SMRiQ12285. Positions 3-56, 72-151, 173-212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34289. 83 interactions.
DIPiDIP-1982N.
IntActiQ12285. 9 interactions.
MINTiMINT-411662.
STRINGi4932.YOL111C.

Proteomic databases

MaxQBiQ12285.
PaxDbiQ12285.
PeptideAtlasiQ12285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL111C; YOL111C; YOL111C.
GeneIDi854038.
KEGGisce:YOL111C.

Organism-specific databases

CYGDiYOL111c.
EuPathDBiFungiDB:YOL111C.
SGDiS000005471. MDY2.

Phylogenomic databases

eggNOGiNOG138422.
HOGENOMiHOG000113515.
InParanoidiQ12285.
OMAiGKVLHDN.
OrthoDBiEOG7XH723.

Enzyme and pathway databases

BioCyciYEAST:G3O-33508-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12285.
NextBioi975601.
PROiQ12285.

Gene expression databases

GenevestigatoriQ12285.

Family and domain databases

InterProiIPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w (TRF4), YOL114c, YOL112w (MSB4), YOL111c and YOL072w."
    Iwanejko L., Smith K.N., Loeillet S., Nicolas A., Fabre F.
    Yeast 15:1529-1539(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae."
    Hu Z., Potthoff B., Hollenberg C.P., Ramezani-Rad M.
    J. Cell Sci. 119:326-338(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1."
    Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C.
    J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 1-59 IN COMPLEX WITH GET4, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMDY2_YEAST
AccessioniPrimary (citable) accession number: Q12285
Secondary accession number(s): D6W1V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.