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Protein

Ubiquitin-like protein MDY2

Gene

MDY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.2 Publications

GO - Biological processi

  • cell morphogenesis involved in conjugation with cellular fusion Source: SGD
  • posttranslational protein targeting to membrane Source: SGD
  • protein insertion into ER membrane Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33508-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein MDY2
Alternative name(s):
Golgi to ER traffic protein 5
Mating-deficient protein 2
Translation machinery-associated protein 24
Gene namesi
Name:MDY2
Synonyms:GET5, TMA24
Ordered Locus Names:YOL111C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL111C.
SGDiS000005471. MDY2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • nucleus Source: SGD
  • TRC complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002359171 – 212Ubiquitin-like protein MDY2Add BLAST212

Proteomic databases

MaxQBiQ12285.
PRIDEiQ12285.

Interactioni

Subunit structurei

Interacts with GET4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121543EBI-34904,EBI-2989
GET4Q121258EBI-34904,EBI-36940
SGT2Q121189EBI-34904,EBI-31784
YDJ1P254912EBI-34904,EBI-10420

Protein-protein interaction databases

BioGridi34289. 86 interactors.
DIPiDIP-1982N.
IntActiQ12285. 9 interactors.
MINTiMINT-411662.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Helixi8 – 20Combined sources13
Helixi35 – 37Combined sources3
Beta strandi74 – 80Combined sources7
Beta strandi82 – 84Combined sources3
Beta strandi86 – 92Combined sources7
Helixi98 – 107Combined sources10
Helixi114 – 116Combined sources3
Beta strandi117 – 121Combined sources5
Beta strandi124 – 126Combined sources3
Helixi132 – 134Combined sources3
Beta strandi139 – 141Combined sources3
Beta strandi143 – 148Combined sources6
Helixi179 – 189Combined sources11
Turni190 – 192Combined sources3
Helixi194 – 210Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LNZNMR-A/B152-212[»]
2LXANMR-A74-151[»]
2LXCNMR-A74-151[»]
2WPVX-ray1.99B/D/F/H1-59[»]
3LKUX-ray2.80B/D/F3-56[»]
3VEJX-ray1.23A/B175-212[»]
3ZDMX-ray1.80C/F71-151[»]
4A20X-ray1.78A70-152[»]
4ASWNMR-C70-152[»]
4GOCX-ray2.40A/B/C74-148[»]
4PWXX-ray5.40D/F1-54[»]
5BW8X-ray2.80D1-54[»]
5BWKX-ray6.00F/H/J/L/R/T/V/X1-56[»]
ProteinModelPortaliQ12285.
SMRiQ12285.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12285.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 152Ubiquitin-likePROSITE-ProRule annotationAdd BLAST79

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000113515.
InParanoidiQ12285.
OMAiPEHEFVS.
OrthoDBiEOG092C4PDD.

Family and domain databases

InterProiIPR031765. Mdy2_get4-bd.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF16843. Get5_bdg. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12285-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY
60 70 80 90 100
KYKQNRAKKL KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ
110 120 130 140 150
IKQHLISEEK ASHISEIKLL LKGKVLHDNL FLSDLKVTPA NSTITVMIKP
160 170 180 190 200
NPTISKEPEA EKSTNSPAPA PPQELTVPWD DIEALLKNNF ENDQAAVRQV
210
MERLQKGWSL AK
Length:212
Mass (Da):23,732
Last modified:November 1, 1996 - v1
Checksum:iCE6FC4282E0E472C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88151.1.
Z74853 Genomic DNA. Translation: CAA99130.1.
BK006948 Genomic DNA. Translation: DAA10672.1.
PIRiS51888.
RefSeqiNP_014530.1. NM_001183365.1.

Genome annotation databases

EnsemblFungiiYOL111C; YOL111C; YOL111C.
GeneIDi854038.
KEGGisce:YOL111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA. Translation: CAA88151.1.
Z74853 Genomic DNA. Translation: CAA99130.1.
BK006948 Genomic DNA. Translation: DAA10672.1.
PIRiS51888.
RefSeqiNP_014530.1. NM_001183365.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LNZNMR-A/B152-212[»]
2LXANMR-A74-151[»]
2LXCNMR-A74-151[»]
2WPVX-ray1.99B/D/F/H1-59[»]
3LKUX-ray2.80B/D/F3-56[»]
3VEJX-ray1.23A/B175-212[»]
3ZDMX-ray1.80C/F71-151[»]
4A20X-ray1.78A70-152[»]
4ASWNMR-C70-152[»]
4GOCX-ray2.40A/B/C74-148[»]
4PWXX-ray5.40D/F1-54[»]
5BW8X-ray2.80D1-54[»]
5BWKX-ray6.00F/H/J/L/R/T/V/X1-56[»]
ProteinModelPortaliQ12285.
SMRiQ12285.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34289. 86 interactors.
DIPiDIP-1982N.
IntActiQ12285. 9 interactors.
MINTiMINT-411662.

Proteomic databases

MaxQBiQ12285.
PRIDEiQ12285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL111C; YOL111C; YOL111C.
GeneIDi854038.
KEGGisce:YOL111C.

Organism-specific databases

EuPathDBiFungiDB:YOL111C.
SGDiS000005471. MDY2.

Phylogenomic databases

HOGENOMiHOG000113515.
InParanoidiQ12285.
OMAiPEHEFVS.
OrthoDBiEOG092C4PDD.

Enzyme and pathway databases

BioCyciYEAST:G3O-33508-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ12285.
PROiQ12285.

Family and domain databases

InterProiIPR031765. Mdy2_get4-bd.
IPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF16843. Get5_bdg. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDY2_YEAST
AccessioniPrimary (citable) accession number: Q12285
Secondary accession number(s): D6W1V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6510 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.