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Q12285

- MDY2_YEAST

UniProt

Q12285 - MDY2_YEAST

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Protein
Ubiquitin-like protein MDY2
Gene
MDY2, GET5, TMA24, YOL111C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.2 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. cell morphogenesis involved in conjugation with cellular fusion Source: SGD
  2. posttranslational protein targeting to membrane Source: SGD
  3. protein insertion into ER membrane Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-33508-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein MDY2
Alternative name(s):
Golgi to ER traffic protein 5
Mating-deficient protein 2
Translation machinery-associated protein 24
Gene namesi
Name:MDY2
Synonyms:GET5, TMA24
Ordered Locus Names:YOL111C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL111c.
SGDiS000005471. MDY2.

Subcellular locationi

Cytoplasmcytosol. Nucleus
Note: Detected in both cytosol and nucleus but is predominantly cytosolic.3 Publications

GO - Cellular componenti

  1. TRC complex Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Ubiquitin-like protein MDY2
PRO_0000235917Add
BLAST

Proteomic databases

MaxQBiQ12285.
PaxDbiQ12285.
PeptideAtlasiQ12285.

Expressioni

Gene expression databases

GenevestigatoriQ12285.

Interactioni

Subunit structurei

Interacts with GET4.

Binary interactionsi

WithEntry#Exp.IntActNotes
GET3Q121543EBI-34904,EBI-2989
GET4Q121258EBI-34904,EBI-36940
SGT2Q121189EBI-34904,EBI-31784
YDJ1P254912EBI-34904,EBI-10420

Protein-protein interaction databases

BioGridi34289. 81 interactions.
DIPiDIP-1982N.
IntActiQ12285. 9 interactions.
MINTiMINT-411662.
STRINGi4932.YOL111C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Helixi8 – 2013
Helixi35 – 373
Beta strandi74 – 807
Beta strandi82 – 843
Beta strandi86 – 927
Helixi98 – 10710
Helixi114 – 1163
Beta strandi117 – 1215
Beta strandi124 – 1263
Helixi132 – 1343
Beta strandi139 – 1413
Beta strandi143 – 1486
Helixi179 – 18911
Turni190 – 1923
Helixi194 – 21017

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LNZNMR-A/B152-212[»]
2LXANMR-A74-151[»]
2LXCNMR-A74-151[»]
2WPVX-ray1.99B/D/F/H1-59[»]
3LKUX-ray2.80B/D/F3-56[»]
3VEJX-ray1.23A/B175-212[»]
3ZDMX-ray1.80C/F71-151[»]
4A20X-ray1.78A70-152[»]
4ASWNMR-C70-152[»]
4GOCX-ray2.40A/B/C74-148[»]
4PWXX-ray5.40D/F1-54[»]
ProteinModelPortaliQ12285.
SMRiQ12285. Positions 3-56, 72-151, 173-212.

Miscellaneous databases

EvolutionaryTraceiQ12285.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 15279Ubiquitin-like
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG138422.
HOGENOMiHOG000113515.
OMAiEWHARLL.
OrthoDBiEOG7XH723.

Family and domain databases

InterProiIPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12285-1 [UniParc]FASTAAdd to Basket

« Hide

MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY    50
KYKQNRAKKL KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ 100
IKQHLISEEK ASHISEIKLL LKGKVLHDNL FLSDLKVTPA NSTITVMIKP 150
NPTISKEPEA EKSTNSPAPA PPQELTVPWD DIEALLKNNF ENDQAAVRQV 200
MERLQKGWSL AK 212
Length:212
Mass (Da):23,732
Last modified:November 1, 1996 - v1
Checksum:iCE6FC4282E0E472C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48149 Genomic DNA. Translation: CAA88151.1.
Z74853 Genomic DNA. Translation: CAA99130.1.
BK006948 Genomic DNA. Translation: DAA10672.1.
PIRiS51888.
RefSeqiNP_014530.1. NM_001183365.1.

Genome annotation databases

EnsemblFungiiYOL111C; YOL111C; YOL111C.
GeneIDi854038.
KEGGisce:YOL111C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48149 Genomic DNA. Translation: CAA88151.1 .
Z74853 Genomic DNA. Translation: CAA99130.1 .
BK006948 Genomic DNA. Translation: DAA10672.1 .
PIRi S51888.
RefSeqi NP_014530.1. NM_001183365.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LNZ NMR - A/B 152-212 [» ]
2LXA NMR - A 74-151 [» ]
2LXC NMR - A 74-151 [» ]
2WPV X-ray 1.99 B/D/F/H 1-59 [» ]
3LKU X-ray 2.80 B/D/F 3-56 [» ]
3VEJ X-ray 1.23 A/B 175-212 [» ]
3ZDM X-ray 1.80 C/F 71-151 [» ]
4A20 X-ray 1.78 A 70-152 [» ]
4ASW NMR - C 70-152 [» ]
4GOC X-ray 2.40 A/B/C 74-148 [» ]
4PWX X-ray 5.40 D/F 1-54 [» ]
ProteinModelPortali Q12285.
SMRi Q12285. Positions 3-56, 72-151, 173-212.
ModBasei Search...

Protein-protein interaction databases

BioGridi 34289. 81 interactions.
DIPi DIP-1982N.
IntActi Q12285. 9 interactions.
MINTi MINT-411662.
STRINGi 4932.YOL111C.

Proteomic databases

MaxQBi Q12285.
PaxDbi Q12285.
PeptideAtlasi Q12285.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL111C ; YOL111C ; YOL111C .
GeneIDi 854038.
KEGGi sce:YOL111C.

Organism-specific databases

CYGDi YOL111c.
SGDi S000005471. MDY2.

Phylogenomic databases

eggNOGi NOG138422.
HOGENOMi HOG000113515.
OMAi EWHARLL.
OrthoDBi EOG7XH723.

Enzyme and pathway databases

BioCyci YEAST:G3O-33508-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12285.
NextBioi 975601.

Gene expression databases

Genevestigatori Q12285.

Family and domain databases

InterProi IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00240. ubiquitin. 1 hit.
[Graphical view ]
SMARTi SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
PROSITEi PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w (TRF4), YOL114c, YOL112w (MSB4), YOL111c and YOL072w."
    Iwanejko L., Smith K.N., Loeillet S., Nicolas A., Fabre F.
    Yeast 15:1529-1539(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae."
    Hu Z., Potthoff B., Hollenberg C.P., Ramezani-Rad M.
    J. Cell Sci. 119:326-338(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1."
    Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C.
    J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 1-59 IN COMPLEX WITH GET4, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiMDY2_YEAST
AccessioniPrimary (citable) accession number: Q12285
Secondary accession number(s): D6W1V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6510 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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