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Q12285

- MDY2_YEAST

UniProt

Q12285 - MDY2_YEAST

Protein

Ubiquitin-like protein MDY2

Gene

MDY2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell morphogenesis involved in conjugation with cellular fusion Source: SGD
    2. posttranslational protein targeting to membrane Source: SGD
    3. protein insertion into ER membrane Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33508-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-like protein MDY2
    Alternative name(s):
    Golgi to ER traffic protein 5
    Mating-deficient protein 2
    Translation machinery-associated protein 24
    Gene namesi
    Name:MDY2
    Synonyms:GET5, TMA24
    Ordered Locus Names:YOL111C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL111c.
    SGDiS000005471. MDY2.

    Subcellular locationi

    Cytoplasmcytosol. Nucleus
    Note: Detected in both cytosol and nucleus but is predominantly cytosolic.

    GO - Cellular componenti

    1. nucleus Source: SGD
    2. TRC complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 212212Ubiquitin-like protein MDY2PRO_0000235917Add
    BLAST

    Proteomic databases

    MaxQBiQ12285.
    PaxDbiQ12285.
    PeptideAtlasiQ12285.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12285.

    Interactioni

    Subunit structurei

    Interacts with GET4.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GET3Q121543EBI-34904,EBI-2989
    GET4Q121258EBI-34904,EBI-36940
    SGT2Q121189EBI-34904,EBI-31784
    YDJ1P254912EBI-34904,EBI-10420

    Protein-protein interaction databases

    BioGridi34289. 81 interactions.
    DIPiDIP-1982N.
    IntActiQ12285. 9 interactions.
    MINTiMINT-411662.
    STRINGi4932.YOL111C.

    Structurei

    Secondary structure

    1
    212
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 63
    Helixi8 – 2013
    Helixi35 – 373
    Beta strandi74 – 807
    Beta strandi82 – 843
    Beta strandi86 – 927
    Helixi98 – 10710
    Helixi114 – 1163
    Beta strandi117 – 1215
    Beta strandi124 – 1263
    Helixi132 – 1343
    Beta strandi139 – 1413
    Beta strandi143 – 1486
    Helixi179 – 18911
    Turni190 – 1923
    Helixi194 – 21017

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2LNZNMR-A/B152-212[»]
    2LXANMR-A74-151[»]
    2LXCNMR-A74-151[»]
    2WPVX-ray1.99B/D/F/H1-59[»]
    3LKUX-ray2.80B/D/F3-56[»]
    3VEJX-ray1.23A/B175-212[»]
    3ZDMX-ray1.80C/F71-151[»]
    4A20X-ray1.78A70-152[»]
    4ASWNMR-C70-152[»]
    4GOCX-ray2.40A/B/C74-148[»]
    4PWXX-ray5.40D/F1-54[»]
    ProteinModelPortaliQ12285.
    SMRiQ12285. Positions 3-56, 72-151, 173-212.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12285.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini74 – 15279Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG138422.
    HOGENOMiHOG000113515.
    OMAiEWHARLL.
    OrthoDBiEOG7XH723.

    Family and domain databases

    InterProiIPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00240. ubiquitin. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS50053. UBIQUITIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12285-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTSASGPEH EFVSKFLTLA TLTEPKLPKS YTKPLKDVTN LGVPLPTLKY    50
    KYKQNRAKKL KLHQDQQGQD NAAVHLTLKK IQAPKFSIEH DFSPSDTILQ 100
    IKQHLISEEK ASHISEIKLL LKGKVLHDNL FLSDLKVTPA NSTITVMIKP 150
    NPTISKEPEA EKSTNSPAPA PPQELTVPWD DIEALLKNNF ENDQAAVRQV 200
    MERLQKGWSL AK 212
    Length:212
    Mass (Da):23,732
    Last modified:November 1, 1996 - v1
    Checksum:iCE6FC4282E0E472C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48149 Genomic DNA. Translation: CAA88151.1.
    Z74853 Genomic DNA. Translation: CAA99130.1.
    BK006948 Genomic DNA. Translation: DAA10672.1.
    PIRiS51888.
    RefSeqiNP_014530.1. NM_001183365.1.

    Genome annotation databases

    EnsemblFungiiYOL111C; YOL111C; YOL111C.
    GeneIDi854038.
    KEGGisce:YOL111C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48149 Genomic DNA. Translation: CAA88151.1 .
    Z74853 Genomic DNA. Translation: CAA99130.1 .
    BK006948 Genomic DNA. Translation: DAA10672.1 .
    PIRi S51888.
    RefSeqi NP_014530.1. NM_001183365.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2LNZ NMR - A/B 152-212 [» ]
    2LXA NMR - A 74-151 [» ]
    2LXC NMR - A 74-151 [» ]
    2WPV X-ray 1.99 B/D/F/H 1-59 [» ]
    3LKU X-ray 2.80 B/D/F 3-56 [» ]
    3VEJ X-ray 1.23 A/B 175-212 [» ]
    3ZDM X-ray 1.80 C/F 71-151 [» ]
    4A20 X-ray 1.78 A 70-152 [» ]
    4ASW NMR - C 70-152 [» ]
    4GOC X-ray 2.40 A/B/C 74-148 [» ]
    4PWX X-ray 5.40 D/F 1-54 [» ]
    ProteinModelPortali Q12285.
    SMRi Q12285. Positions 3-56, 72-151, 173-212.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34289. 81 interactions.
    DIPi DIP-1982N.
    IntActi Q12285. 9 interactions.
    MINTi MINT-411662.
    STRINGi 4932.YOL111C.

    Proteomic databases

    MaxQBi Q12285.
    PaxDbi Q12285.
    PeptideAtlasi Q12285.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL111C ; YOL111C ; YOL111C .
    GeneIDi 854038.
    KEGGi sce:YOL111C.

    Organism-specific databases

    CYGDi YOL111c.
    SGDi S000005471. MDY2.

    Phylogenomic databases

    eggNOGi NOG138422.
    HOGENOMi HOG000113515.
    OMAi EWHARLL.
    OrthoDBi EOG7XH723.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33508-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12285.
    NextBioi 975601.

    Gene expression databases

    Genevestigatori Q12285.

    Family and domain databases

    InterProi IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00240. ubiquitin. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS50053. UBIQUITIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
      Vandenbol M., Durand P., Portetelle D., Hilger F.
      Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w (TRF4), YOL114c, YOL112w (MSB4), YOL111c and YOL072w."
      Iwanejko L., Smith K.N., Loeillet S., Nicolas A., Fabre F.
      Yeast 15:1529-1539(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae."
      Hu Z., Potthoff B., Hollenberg C.P., Ramezani-Rad M.
      J. Cell Sci. 119:326-338(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1."
      Chang Y.W., Chuang Y.C., Ho Y.C., Cheng M.Y., Sun Y.J., Hsiao C.D., Wang C.
      J. Biol. Chem. 285:9962-9970(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS) OF 1-59 IN COMPLEX WITH GET4, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiMDY2_YEAST
    AccessioniPrimary (citable) accession number: Q12285
    Secondary accession number(s): D6W1V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2006
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3