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Q12284

- ERV2_YEAST

UniProt

Q12284 - ERV2_YEAST

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Protein

FAD-linked sulfhydryl oxidase ERV2

Gene

ERV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1.2 PublicationsPROSITE-ProRule annotation

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD.2 PublicationsPROSITE-ProRule annotation

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: SGD
  2. thiol oxidase activity Source: SGD

GO - Biological processi

  1. oxidation-reduction process Source: SGD
  2. protein folding Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-34195-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ERV2 (EC:1.8.3.2)
Gene namesi
Name:ERV2
Ordered Locus Names:YPR037C
ORF Names:YP3085.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR037c.
SGDiS000006241. ERV2.

Subcellular locationi

Endoplasmic reticulum membrane 2 Publications; Single-pass type III membrane protein 2 Publications; Lumenal side 2 Publications

GO - Cellular componenti

  1. integral component of endoplasmic reticulum membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi121 – 1211C → A: Loss of function. 1 Publication
Mutagenesisi124 – 1241C → A: Loss of function. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196FAD-linked sulfhydryl oxidase ERV2PRO_0000001188Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi121 ↔ 124Redox-activePROSITE-ProRule annotation
Disulfide bondi150 ↔ 1671 PublicationPROSITE-ProRule annotation
Disulfide bondi176 ↔ 1781 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ12284.
PaxDbiQ12284.
PeptideAtlasiQ12284.

Expressioni

Gene expression databases

GenevestigatoriQ12284.

Interactioni

Subunit structurei

Homodimer. Interacts with the substrate protein PDI1, forming transient intermolecular disulfide bridges.2 Publications

Protein-protein interaction databases

BioGridi36216. 18 interactions.
DIPiDIP-3906N.
IntActiQ12284. 1 interaction.
MINTiMINT-560963.
STRINGi4932.YPR037C.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi75 – 9420
Helixi102 – 11817
Helixi122 – 13413
Helixi142 – 15918
Helixi170 – 1734

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JR8X-ray1.50A/B71-187[»]
1JRAX-ray2.00A/B/C/D71-187[»]
ProteinModelPortaliQ12284.
SMRiQ12284. Positions 74-179.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12284.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini36 – 196161LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3523Helical; Signal-anchorSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini72 – 174103ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 869FAD-binding
Regioni153 – 17422FAD-bindingAdd
BLAST

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5054.
HOGENOMiHOG000180458.
InParanoidiQ12284.
KOiK17891.
OMAiGRASWKY.
OrthoDBiEOG7WDNCT.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12284-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQIVKRSHA IRIVAALGII GLWMFFSSNE LSIATPGLIK AKSGIDEVQG
60 70 80 90 100
AAAEKNDARL KEIEKQTIMP LMGDDKVKKE VGRASWKYFH TLLARFPDEP
110 120 130 140 150
TPEEREKLHT FIGLYAELYP CGECSYHFVK LIEKYPVQTS SRTAAAMWGC
160 170 180 190
HIHNKVNEYL KKDIYDCATI LEDYDCGCSD SDGKRVSLEK EAKQHG
Length:196
Mass (Da):22,141
Last modified:November 1, 1997 - v1
Checksum:i7FEE76B5F01D3D65
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71255 Genomic DNA. Translation: CAA94987.1.
Z68111 Genomic DNA. Translation: CAA92143.1.
BK006949 Genomic DNA. Translation: DAA11464.1.
PIRiS61060.
RefSeqiNP_015362.1. NM_001184134.1.

Genome annotation databases

EnsemblFungiiYPR037C; YPR037C; YPR037C.
GeneIDi856152.
KEGGisce:YPR037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z71255 Genomic DNA. Translation: CAA94987.1 .
Z68111 Genomic DNA. Translation: CAA92143.1 .
BK006949 Genomic DNA. Translation: DAA11464.1 .
PIRi S61060.
RefSeqi NP_015362.1. NM_001184134.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JR8 X-ray 1.50 A/B 71-187 [» ]
1JRA X-ray 2.00 A/B/C/D 71-187 [» ]
ProteinModelPortali Q12284.
SMRi Q12284. Positions 74-179.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36216. 18 interactions.
DIPi DIP-3906N.
IntActi Q12284. 1 interaction.
MINTi MINT-560963.
STRINGi 4932.YPR037C.

Proteomic databases

MaxQBi Q12284.
PaxDbi Q12284.
PeptideAtlasi Q12284.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR037C ; YPR037C ; YPR037C .
GeneIDi 856152.
KEGGi sce:YPR037C.

Organism-specific databases

CYGDi YPR037c.
SGDi S000006241. ERV2.

Phylogenomic databases

eggNOGi COG5054.
HOGENOMi HOG000180458.
InParanoidi Q12284.
KOi K17891.
OMAi GRASWKY.
OrthoDBi EOG7WDNCT.

Enzyme and pathway databases

BioCyci YEAST:G3O-34195-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q12284.
NextBioi 981280.

Gene expression databases

Genevestigatori Q12284.

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view ]
Pfami PF04777. Evr1_Alr. 1 hit.
[Graphical view ]
SUPFAMi SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Functional comparison of the yeast scERV1 and scERV2 genes."
    Stein G., Lisowsky T.
    Yeast 14:171-180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Yeast ERV2p is the first microsomal FAD-linked sulfhydryl oxidase of the Erv1p/Alrp protein family."
    Gerber J., Muehlenhoff U., Hofhaus G., Lill R., Lisowsky T.
    J. Biol. Chem. 276:23486-23491(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, FAD-BINDING, SUBUNIT.
  5. "A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation."
    Sevier C.S., Cuozzo J.W., Vala A., Aaslund F., Kaiser C.A.
    Nat. Cell Biol. 3:874-882(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, FAD-BINDING, MUTAGENESIS OF CYS-121 AND CYS-124, SUBUNIT, INTERACTION WITH PDI1.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p."
    Gross E., Sevier C.S., Vala A., Kaiser C.A., Fass D.
    Nat. Struct. Biol. 9:61-67(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 71-187, DISULFIDE BONDS.

Entry informationi

Entry nameiERV2_YEAST
AccessioniPrimary (citable) accession number: Q12284
Secondary accession number(s): D6W448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3