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Protein

FAD-linked sulfhydryl oxidase ERV2

Gene

ERV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1.PROSITE-ProRule annotation2 Publications

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

  • oxidation-reduction process Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-34195-MONOMER
BRENDAi1.8.3.2 984

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ERV2 (EC:1.8.3.2)
Gene namesi
Name:ERV2
Ordered Locus Names:YPR037C
ORF Names:YP3085.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR037C
SGDiS000006241 ERV2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchorSequence analysisAdd BLAST23
Topological domaini36 – 196LumenalSequence analysisAdd BLAST161

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi121C → A: Loss of function. 1 Publication1
Mutagenesisi124C → A: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000011881 – 196FAD-linked sulfhydryl oxidase ERV2Add BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi121 ↔ 124Redox-activePROSITE-ProRule annotation
Disulfide bondi150 ↔ 167PROSITE-ProRule annotation1 Publication
Disulfide bondi176 ↔ 178PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ12284
PaxDbiQ12284
PRIDEiQ12284

PTM databases

iPTMnetiQ12284

Interactioni

Subunit structurei

Homodimer. Interacts with the substrate protein PDI1, forming transient intermolecular disulfide bridges.2 Publications

Protein-protein interaction databases

BioGridi36216, 50 interactors
DIPiDIP-3906N
IntActiQ12284, 1 interactor
MINTiQ12284
STRINGi4932.YPR037C

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi75 – 94Combined sources20
Helixi102 – 118Combined sources17
Helixi122 – 134Combined sources13
Helixi142 – 159Combined sources18
Helixi170 – 173Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JR8X-ray1.50A/B71-187[»]
1JRAX-ray2.00A/B/C/D71-187[»]
ProteinModelPortaliQ12284
SMRiQ12284
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12284

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 174ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 86FAD-binding9
Regioni153 – 174FAD-bindingAdd BLAST22

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000180458
InParanoidiQ12284
KOiK17891
OMAiRASWKYF
OrthoDBiEOG092C3SAY

Family and domain databases

Gene3Di1.20.120.310, 1 hit
InterProiView protein in InterPro
IPR036774 ERV/ALR_sulphydryl_oxid_sf
IPR017905 ERV/ALR_sulphydryl_oxidase
PfamiView protein in Pfam
PF04777 Evr1_Alr, 1 hit
SUPFAMiSSF69000 SSF69000, 1 hit
PROSITEiView protein in PROSITE
PS51324 ERV_ALR, 1 hit

Sequencei

Sequence statusi: Complete.

Q12284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQIVKRSHA IRIVAALGII GLWMFFSSNE LSIATPGLIK AKSGIDEVQG
60 70 80 90 100
AAAEKNDARL KEIEKQTIMP LMGDDKVKKE VGRASWKYFH TLLARFPDEP
110 120 130 140 150
TPEEREKLHT FIGLYAELYP CGECSYHFVK LIEKYPVQTS SRTAAAMWGC
160 170 180 190
HIHNKVNEYL KKDIYDCATI LEDYDCGCSD SDGKRVSLEK EAKQHG
Length:196
Mass (Da):22,141
Last modified:November 1, 1997 - v1
Checksum:i7FEE76B5F01D3D65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA Translation: CAA94987.1
Z68111 Genomic DNA Translation: CAA92143.1
BK006949 Genomic DNA Translation: DAA11464.1
PIRiS61060
RefSeqiNP_015362.1, NM_001184134.1

Genome annotation databases

EnsemblFungiiYPR037C; YPR037C; YPR037C
GeneIDi856152
KEGGisce:YPR037C

Similar proteinsi

Entry informationi

Entry nameiERV2_YEAST
AccessioniPrimary (citable) accession number: Q12284
Secondary accession number(s): D6W448
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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