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Protein

FAD-linked sulfhydryl oxidase ERV2

Gene

ERV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1.PROSITE-ProRule annotation2 Publications

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADPROSITE-ProRule annotation2 Publications

GO - Molecular functioni

  • flavin adenine dinucleotide binding Source: GO_Central
  • flavin-linked sulfhydryl oxidase activity Source: GO_Central
  • protein disulfide isomerase activity Source: SGD
  • protein disulfide oxidoreductase activity Source: GO_Central
  • thiol oxidase activity Source: SGD

GO - Biological processi

  • oxidation-reduction process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciYEAST:G3O-34195-MONOMER.
BRENDAi1.8.3.2. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
FAD-linked sulfhydryl oxidase ERV2 (EC:1.8.3.2)
Gene namesi
Name:ERV2
Ordered Locus Names:YPR037C
ORF Names:YP3085.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR037C.
SGDiS000006241. ERV2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 35Helical; Signal-anchorSequence analysisAdd BLAST23
Topological domaini36 – 196LumenalSequence analysisAdd BLAST161

GO - Cellular componenti

  • integral component of endoplasmic reticulum membrane Source: SGD
  • mitochondrion Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi121C → A: Loss of function. 1 Publication1
Mutagenesisi124C → A: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000011881 – 196FAD-linked sulfhydryl oxidase ERV2Add BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi121 ↔ 124Redox-activePROSITE-ProRule annotation
Disulfide bondi150 ↔ 167PROSITE-ProRule annotation1 Publication
Disulfide bondi176 ↔ 178PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ12284.
PRIDEiQ12284.

PTM databases

iPTMnetiQ12284.

Interactioni

Subunit structurei

Homodimer. Interacts with the substrate protein PDI1, forming transient intermolecular disulfide bridges.2 Publications

Protein-protein interaction databases

BioGridi36216. 17 interactors.
DIPiDIP-3906N.
IntActiQ12284. 1 interactor.
MINTiMINT-560963.

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi75 – 94Combined sources20
Helixi102 – 118Combined sources17
Helixi122 – 134Combined sources13
Helixi142 – 159Combined sources18
Helixi170 – 173Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JR8X-ray1.50A/B71-187[»]
1JRAX-ray2.00A/B/C/D71-187[»]
ProteinModelPortaliQ12284.
SMRiQ12284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12284.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 174ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST103

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 86FAD-binding9
Regioni153 – 174FAD-bindingAdd BLAST22

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000180458.
InParanoidiQ12284.
KOiK17891.
OMAiGRASWKY.
OrthoDBiEOG092C3SAY.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQIVKRSHA IRIVAALGII GLWMFFSSNE LSIATPGLIK AKSGIDEVQG
60 70 80 90 100
AAAEKNDARL KEIEKQTIMP LMGDDKVKKE VGRASWKYFH TLLARFPDEP
110 120 130 140 150
TPEEREKLHT FIGLYAELYP CGECSYHFVK LIEKYPVQTS SRTAAAMWGC
160 170 180 190
HIHNKVNEYL KKDIYDCATI LEDYDCGCSD SDGKRVSLEK EAKQHG
Length:196
Mass (Da):22,141
Last modified:November 1, 1997 - v1
Checksum:i7FEE76B5F01D3D65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94987.1.
Z68111 Genomic DNA. Translation: CAA92143.1.
BK006949 Genomic DNA. Translation: DAA11464.1.
PIRiS61060.
RefSeqiNP_015362.1. NM_001184134.1.

Genome annotation databases

EnsemblFungiiYPR037C; YPR037C; YPR037C.
GeneIDi856152.
KEGGisce:YPR037C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z71255 Genomic DNA. Translation: CAA94987.1.
Z68111 Genomic DNA. Translation: CAA92143.1.
BK006949 Genomic DNA. Translation: DAA11464.1.
PIRiS61060.
RefSeqiNP_015362.1. NM_001184134.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JR8X-ray1.50A/B71-187[»]
1JRAX-ray2.00A/B/C/D71-187[»]
ProteinModelPortaliQ12284.
SMRiQ12284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36216. 17 interactors.
DIPiDIP-3906N.
IntActiQ12284. 1 interactor.
MINTiMINT-560963.

PTM databases

iPTMnetiQ12284.

Proteomic databases

MaxQBiQ12284.
PRIDEiQ12284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR037C; YPR037C; YPR037C.
GeneIDi856152.
KEGGisce:YPR037C.

Organism-specific databases

EuPathDBiFungiDB:YPR037C.
SGDiS000006241. ERV2.

Phylogenomic databases

HOGENOMiHOG000180458.
InParanoidiQ12284.
KOiK17891.
OMAiGRASWKY.
OrthoDBiEOG092C3SAY.

Enzyme and pathway databases

BioCyciYEAST:G3O-34195-MONOMER.
BRENDAi1.8.3.2. 984.

Miscellaneous databases

EvolutionaryTraceiQ12284.
PROiQ12284.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
[Graphical view]
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERV2_YEAST
AccessioniPrimary (citable) accession number: Q12284
Secondary accession number(s): D6W448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.