Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q12284

- ERV2_YEAST

UniProt

Q12284 - ERV2_YEAST

Protein

FAD-linked sulfhydryl oxidase ERV2

Gene

ERV2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation in the endoplasmic reticulum lumen in parallel to ERO1.2 PublicationsPROSITE-ProRule annotation

    Catalytic activityi

    2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

    Cofactori

    FAD.2 PublicationsPROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: SGD
    2. thiol oxidase activity Source: SGD

    GO - Biological processi

    1. oxidation-reduction process Source: SGD
    2. protein folding Source: GOC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34195-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    FAD-linked sulfhydryl oxidase ERV2 (EC:1.8.3.2)
    Gene namesi
    Name:ERV2
    Ordered Locus Names:YPR037C
    ORF Names:YP3085.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR037c.
    SGDiS000006241. ERV2.

    Subcellular locationi

    Endoplasmic reticulum membrane 2 Publications; Single-pass type III membrane protein 2 Publications; Lumenal side 2 Publications

    GO - Cellular componenti

    1. integral component of endoplasmic reticulum membrane Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi121 – 1211C → A: Loss of function. 1 Publication
    Mutagenesisi124 – 1241C → A: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 196196FAD-linked sulfhydryl oxidase ERV2PRO_0000001188Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi121 ↔ 124Redox-activePROSITE-ProRule annotation
    Disulfide bondi150 ↔ 1671 PublicationPROSITE-ProRule annotation
    Disulfide bondi176 ↔ 1781 PublicationPROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ12284.
    PaxDbiQ12284.
    PeptideAtlasiQ12284.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12284.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with the substrate protein PDI1, forming transient intermolecular disulfide bridges.2 Publications

    Protein-protein interaction databases

    BioGridi36216. 18 interactions.
    DIPiDIP-3906N.
    IntActiQ12284. 1 interaction.
    MINTiMINT-560963.
    STRINGi4932.YPR037C.

    Structurei

    Secondary structure

    1
    196
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi75 – 9420
    Helixi102 – 11817
    Helixi122 – 13413
    Helixi142 – 15918
    Helixi170 – 1734

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JR8X-ray1.50A/B71-187[»]
    1JRAX-ray2.00A/B/C/D71-187[»]
    ProteinModelPortaliQ12284.
    SMRiQ12284. Positions 74-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ12284.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 196161LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3523Helical; Signal-anchorSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini72 – 174103ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni78 – 869FAD-binding
    Regioni153 – 17422FAD-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5054.
    HOGENOMiHOG000180458.
    KOiK17891.
    OMAiGRASWKY.
    OrthoDBiEOG7WDNCT.

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    [Graphical view]
    PfamiPF04777. Evr1_Alr. 1 hit.
    [Graphical view]
    SUPFAMiSSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12284-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQIVKRSHA IRIVAALGII GLWMFFSSNE LSIATPGLIK AKSGIDEVQG    50
    AAAEKNDARL KEIEKQTIMP LMGDDKVKKE VGRASWKYFH TLLARFPDEP 100
    TPEEREKLHT FIGLYAELYP CGECSYHFVK LIEKYPVQTS SRTAAAMWGC 150
    HIHNKVNEYL KKDIYDCATI LEDYDCGCSD SDGKRVSLEK EAKQHG 196
    Length:196
    Mass (Da):22,141
    Last modified:November 1, 1997 - v1
    Checksum:i7FEE76B5F01D3D65
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71255 Genomic DNA. Translation: CAA94987.1.
    Z68111 Genomic DNA. Translation: CAA92143.1.
    BK006949 Genomic DNA. Translation: DAA11464.1.
    PIRiS61060.
    RefSeqiNP_015362.1. NM_001184134.1.

    Genome annotation databases

    EnsemblFungiiYPR037C; YPR037C; YPR037C.
    GeneIDi856152.
    KEGGisce:YPR037C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z71255 Genomic DNA. Translation: CAA94987.1 .
    Z68111 Genomic DNA. Translation: CAA92143.1 .
    BK006949 Genomic DNA. Translation: DAA11464.1 .
    PIRi S61060.
    RefSeqi NP_015362.1. NM_001184134.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JR8 X-ray 1.50 A/B 71-187 [» ]
    1JRA X-ray 2.00 A/B/C/D 71-187 [» ]
    ProteinModelPortali Q12284.
    SMRi Q12284. Positions 74-179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36216. 18 interactions.
    DIPi DIP-3906N.
    IntActi Q12284. 1 interaction.
    MINTi MINT-560963.
    STRINGi 4932.YPR037C.

    Proteomic databases

    MaxQBi Q12284.
    PaxDbi Q12284.
    PeptideAtlasi Q12284.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR037C ; YPR037C ; YPR037C .
    GeneIDi 856152.
    KEGGi sce:YPR037C.

    Organism-specific databases

    CYGDi YPR037c.
    SGDi S000006241. ERV2.

    Phylogenomic databases

    eggNOGi COG5054.
    HOGENOMi HOG000180458.
    KOi K17891.
    OMAi GRASWKY.
    OrthoDBi EOG7WDNCT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34195-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q12284.
    NextBioi 981280.

    Gene expression databases

    Genevestigatori Q12284.

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    [Graphical view ]
    Pfami PF04777. Evr1_Alr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Functional comparison of the yeast scERV1 and scERV2 genes."
      Stein G., Lisowsky T.
      Yeast 14:171-180(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Yeast ERV2p is the first microsomal FAD-linked sulfhydryl oxidase of the Erv1p/Alrp protein family."
      Gerber J., Muehlenhoff U., Hofhaus G., Lill R., Lisowsky T.
      J. Biol. Chem. 276:23486-23491(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, FAD-BINDING, SUBUNIT.
    5. "A flavoprotein oxidase defines a new endoplasmic reticulum pathway for biosynthetic disulphide bond formation."
      Sevier C.S., Cuozzo J.W., Vala A., Aaslund F., Kaiser C.A.
      Nat. Cell Biol. 3:874-882(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, FAD-BINDING, MUTAGENESIS OF CYS-121 AND CYS-124, SUBUNIT, INTERACTION WITH PDI1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p."
      Gross E., Sevier C.S., Vala A., Kaiser C.A., Fass D.
      Nat. Struct. Biol. 9:61-67(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 71-187, DISULFIDE BONDS.

    Entry informationi

    Entry nameiERV2_YEAST
    AccessioniPrimary (citable) accession number: Q12284
    Secondary accession number(s): D6W448
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 259 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3