Q12283 (FABD_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malonyl CoA-acyl carrier protein transacylase, mitochondrial
Short name=MCT
EC=2.3.1.39

Alternative name(s):
Malonyl-CoA:ACP transferase

Gene names

Name:	MCT1
Ordered Locus Names:	YOR221C
ORF Names:	YOR50-11

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in biosynthesis of fatty acids in mitochondria By similarity. Ref.4
Catalytic activity	Malonyl-CoA + [acyl-carrier-protein] = CoA + malonyl-[acyl-carrier-protein].
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Mitochondrion Ref.5.
Miscellaneous	Present with 1360 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the FabD family.
Sequence caution	The sequence CAA63184.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA99439.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from mutant phenotype Ref.4. Source: SGD fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway fatty acid metabolic process Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay PubMed 16823961. Source: SGD
Molecular_function	[acyl-carrier-protein] S-malonyltransferase activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Potential
	Chain	25 – 360	336	Malonyl CoA-acyl carrier protein transacylase, mitochondrial		PRO_0000257811
Sites
	Active site	105	1	By similarity
	Active site	235	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12283 [UniParc]. Last modified October 31, 2006. Version 2. Checksum: F27DC450F367F240	FASTA	360	40,707
	10 20 30 40 50 60 MKLLTFPGQG TSISISILKA IIRNKSREFQ TILSQNGKES NDLLQYIFQN PSSPGSIAVC 70 80 90 100 110 120 SNLFYQLYQI LSNPSDPQDQ APKNMTKIDS PDKKDNEQCY LLGHSLGELT CLSVNSLFSL 130 140 150 160 170 180 KDLFDIANFR NKLMVTSTEK YLVAHNINRS NKFEMWALSS PRATDLPQEV QKLLNSPNLL 190 200 210 220 230 240 SSSQNTISVA NANSVKQCVV TGLVDDLESL RTELNLRFPR LRITELTNPY NIPFHNSTVL 250 260 270 280 290 300 RPVQEPLYDY IWDILKKNGT HTLMELNHPI IANLDGNISY YIHHALDRFV KCSSRTVQFT 310 320 330 340 350 360 MCYDTINSGT PVEIDKSICF GPGNVIYNLI RRNCPQVDTI EYTSLATIDA YHKAAEENKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of a 33 kb fragment from the right arm of chromosome XV of the yeast Saccharomyces cerevisiae." Galisson F., Dujon B. Yeast 12:877-885(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Two genes of the putative mitochondrial fatty acid synthase in the genome of Saccharomyces cerevisiae." Schneider R., Brors B., Buerger F., Camrath S., Weiss H. Curr. Genet. 32:384-388(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92441 Genomic DNA. Translation: CAA63184.1. Sequence problems. Z75129 Genomic DNA. Translation: CAA99439.1. Sequence problems. BK006948 Genomic DNA. Translation: DAA10992.1.
PIR	S60948.
RefSeq	NP_014864.4. NM_001183640.3.
3D structure databases
ProteinModelPortal	Q12283.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4125N.
IntAct	Q12283. 5 interactions.
MINT	MINT-564545.
STRING	4932.YOR221C.
Proteomic databases
PaxDb	Q12283.
PeptideAtlas	Q12283.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR221C; YOR221C; YOR221C.
GeneID	854396.
KEGG	sce:YOR221C. sce:YOR223W.
Organism-specific databases
CYGD	YOR221c.
SGD	S000005747. MCT1.
Phylogenomic databases
eggNOG	COG0331.
HOGENOM	HOG000066003.
KO	K00645.
OMA	EMWALSS.
OrthoDB	EOG4HDX3N.
Enzyme and pathway databases
UniPathway	UPA00094.
Gene expression databases
Genevestigator	Q12283.
GermOnline	YOR221C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.366.10. 2 hits.
InterPro	IPR001227. Ac_transferase_dom. IPR016035. Acyl_Trfase/lysoPLipase. [Graphical view]
SUPFAM	SSF52151. Acyl_Trfase/lysoPlipase. 1 hit.
ProtoNet	Search...
Other
NextBio	976562.

Entry information

Entry name

FABD_YEAST

Accession

Primary (citable) accession number: Q12283
Secondary accession number(s): D6W2S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 31, 2006
Last modified:	April 3, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program