Q12280 (IQG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating-like protein IQG1

Alternative name(s):
Cytokinesis protein 1
IQGAP-related protein 1

Gene names

Name:	IQG1
Synonyms:	CYK1
Ordered Locus Names:	YPL242C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1495 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the assembly and the contraction of the actomyosin ring at the bud neck during cytokinesis. Seems to be involved in additional tasks during cell division like axial bud-site selection and targeted secretion by recruiting the spatial landmark BUD4, the septin CDC12 and the secretion landmark SEC3 to the bud neck. May be regulated by calcium ions. Ref.4 Ref.5 Ref.6 Ref.13
Subunit structure	Interacts with AFR1, AKR1, activated CDC42, calmodulin (CMD1), myosin MYO1 and its light chain MLC1, BUD4, INN1, SEC3 and TEM1. Ref.1 Ref.6 Ref.7 Ref.8 Ref.13
Subcellular location	Bud neck. Note: Forms a ring at the bud neck in a MLC1-dependent manner, which contracts at the end of cytokinesis. Ref.5 Ref.9 Ref.13
Domain	The calponin homology (CH) domain binds to actin filaments and is required for their recruitment to the bud neck. The IQ domains serve as interaction surface for the myosin light chain MLC1. The Ras-GAP domain is required for contraction of the actomyosin ring and is needed for the interaction with TEM1. It probably does not stimulate GTPase activity.
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. Ref.10
Sequence similarities	Contains 1 CH (calponin-homology) domain. Contains 7 IQ domains. Contains 1 Ras-GAP domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	Coiled coil Repeat
Ligand	Actin-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin filament organization Inferred from physical interaction Ref.6. Source: SGD cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cytokinesis Inferred from mutant phenotype Ref.5. Source: SGD response to osmotic stress Traceable author statement. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cellular bud neck contractile ring Traceable author statement. Source: SGD
Molecular function	Ras GTPase activator activity Inferred from electronic annotation. Source: InterPro actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BUD4	P47136	4	EBI-35351,EBI-3848
MLC1	P53141	7	EBI-35351,EBI-10988

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1495	1495	Ras GTPase-activating-like protein IQG1		PRO_0000056652
Regions
	Domain	108 – 217	110	CH
	Domain	447 – 467	21	IQ 1
	Domain	538 – 567	30	IQ 2
	Domain	568 – 597	30	IQ 3
	Domain	599 – 628	30	IQ 4
	Domain	629 – 658	30	IQ 5
	Domain	687 – 716	30	IQ 6
	Domain	717 – 746	30	IQ 7
	Domain	860 – 1071	212	Ras-GAP
	Coiled coil	759 – 798	40	Potential
	Compositional bias	37 – 76	40	Ser-rich
Amino acid modifications
	Modified residue	354	1	Phosphoserine Ref.11 Ref.12
	Modified residue	365	1	Phosphoserine Ref.12
Experimental info
	Mutagenesis	457	1	L → P in IQG1-1; causes a defect in cytokinesis at 37 degrees Celsius. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	Q12280 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C0C7582808154DCC	FASTA	1,495	172,830
	10 20 30 40 50 60 MTAYSGSPSK PGNNNSYLNR YVENLGTNVT PPLRPQSSSK INSSLNIASP SHLKTKTSAS 70 80 90 100 110 120 NSSATILSKK VESSVSKLKP SLPNKLVGKY TVDLSNYSKI ELRYYEFLCR VSEVKIWIEA 130 140 150 160 170 180 VIEEALPSEI ELCVGDSLRN GVFLAKLTQR INPDLTTVIF PAGDKLQFKH TQNINAFFGL 190 200 210 220 230 240 VEHVGVPDSF RFELQDLYNK KNIPQVFETL HILISMINKK WPGKTPALTN VSGQISFTKE 250 260 270 280 290 300 EIAACKKAWP RIRDFKSLGT NINTAPASPE EPKEKRSGLI KDFNKFERPN IPVEEILITP 310 320 330 340 350 360 RKNITDANCS DFSNTPSPYN EAPKMSNLDV VVEKRKFTPI EPSLLGPTPS LEYSPIKNKS 370 380 390 400 410 420 LSYYSPTISK YLTYDTEFYT RRSRAREEDL NYYQTFKYSP SHYSPMRRER MTEEQFLEKV 430 440 450 460 470 480 VQLQNICRGV NTRFNLYIQK RLLNLFEQDI LRFQACLRGN KFRVLSSMYL PIRRAKIDVP 490 500 510 520 530 540 HVEAIQSRIK GSRIRYKYDK LKFTLSRFSC TVELLQAYCR SKLLKTTVNT KLNDIEISHY 550 560 570 580 590 600 PLTKLQSYMR ASYVRKKVMS LNTKLNDERE SIMKFSAIIR GNVVRCSEDA ILSAVHDVHK 610 620 630 640 650 660 ENISKLQSLI RGIFTRSCLA SIIYSLGKEN CNIIQLSACI RGNAVRHKVQ SLFAPENNLS 670 680 690 700 710 720 ETVHDLQGLV RGILVRYTLD LVDDIVEYNN LALFQAFSRG ALVRESLDQK SSFYKRNVRS 730 740 750 760 770 780 VIMIQSWIRK SLQRSAYLEL LDCPNPSLWA VKKFVHLLNG TATIEEVQNQ LESCQASLDS 790 800 810 820 830 840 ENMKKERLLK SIRQQLNING VLDKFGLLKD KDHELGISDS TIPKSKYQKY EKLFYMLQVD 850 860 870 880 890 900 PSYWKLLYLK EPEFVAKNVY MTFGTVNQRM NDRERSYFTR FVCEMLQNAI NEAPSIESFL 910 920 930 940 950 960 DNRSQFWQTI LQDFLRRESP EFFSIIVPVL DYLSDPVVDF ESDPYKIYQE IHGFSSPQHC 970 980 990 1000 1010 1020 SPVDDASTKN KFIDNLRCLW HAIEMVAEIY TRKVHTIPVE IRYLCTKIFC YAADKNIEEI 1030 1040 1050 1060 1070 1080 DSLRAISSIL VNVFVSEYLV NREYYGYKDS NVQKNNQKID ILMKSLATVF EIKNFDGFLD 1090 1100 1110 1120 1130 1140 PLNQYANEIK PHIKDVLYNV LVDPEYEQEG DRLIYLDMVS PSPKLELLTE KVLEISGKFE 1150 1160 1170 1180 1190 1200 EYLNEFPEAD ILHDILEKNL DNSSFPRSGR VTLELDASAY RFLVSDDKMR KIYDQVKRAF 1210 1220 1230 1240 1250 1260 VYMMQIEDVD TNLYDLSIST ILPQDEPNFA NFLEQNPKIR DDPMIQKLKP LKYFTLKNVT 1270 1280 1290 1300 1310 1320 LKKIHELEST GTFCSSDNKL QNFLNDIANT IKNPNYAIDY VTQEIYITKE TLTKISEMNH 1330 1340 1350 1360 1370 1380 SLDIELSRLK KHVDHTIKDF QKAKDFSPVH KSKFGNFKNA VKKVQGRERS ELQGMKFKWN 1390 1400 1410 1420 1430 1440 TKQLYERGVL KTIRGEKLAE LTVKVFGSSG PKFPDIIFKI STSDGSRFGI QMIDKRKGPD 1450 1460 1470 1480 1490 KRYSDDVDSF SFKDLIKTQV EPKIETWKLF HSNVVVNNSQ LLHLIVSFFY KRNAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton." Osman M.A., Cerione R.A. J. Cell Biol. 142:443-455(1998) [PubMed: 9679143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH AFR1; AKR1 AND CDC42. Strain: CUY30.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"An IQGAP-related protein controls actin-ring formation and cytokinesis in yeast." Epp J.A., Chant J. Curr. Biol. 7:921-929(1997) [PubMed: 9382845] [Abstract] Cited for: FUNCTION.
[5]	"Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis." Lippincott J., Li R. J. Cell Biol. 140:355-366(1998) [PubMed: 9442111] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"The multiple roles of Cyk1p in the assembly and function of the actomyosin ring in budding yeast." Shannon K.B., Li R. Mol. Biol. Cell 10:283-296(1999) [PubMed: 9950677] [Abstract] Cited for: FUNCTION, INTERACTION WITH CMD1 AND TEM1.
[7]	"Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis." Boyne J.R., Yosuf H.M., Bieganowski P., Brenner C., Price C. J. Cell Sci. 113:4533-4543(2000) [PubMed: 11082046] [Abstract] Cited for: INTERACTION WITH MLC1 AND MYO1, MUTAGENESIS OF LEU-457.
[8]	"Iqg1p links spatial and secretion landmarks to polarity and cytokinesis." Osman M.A., Konopka J.B., Cerione R.A. J. Cell Biol. 159:601-611(2002) [PubMed: 12446742] [Abstract] Cited for: INTERACTION WITH BUD4 AND SEC3.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-354 AND SER-365, MASS SPECTROMETRY.
[13]	"Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast." Sanchez-Diaz A., Marchesi V., Murray S., Jones R., Pereira G., Edmondson R., Allen T., Labib K. Nat. Cell Biol. 10:395-406(2008) [PubMed: 18344988] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH INN1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019644 Genomic DNA. Translation: AAB70827.1. Z73598 Genomic DNA. Translation: CAA97963.1. Z67751 Genomic DNA. Translation: CAA91603.1. BK006949 Genomic DNA. Translation: DAA11195.1.
PIR	S61023.
RefSeq	NP_015082.1. NM_001184056.1.
3D structure databases
ProteinModelPortal	Q12280.
SMR	Q12280. Positions 99-258, 1369-1494.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1144N.
IntAct	Q12280. 12 interactions.
MINT	MINT-1578204.
STRING	Q12280.
Proteomic databases
PeptideAtlas	Q12280.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL242C; YPL242C; YPL242C.
GeneID	855834.
KEGG	sce:YPL242C.
NMPDR	fig|4932.3.peg.6209.
Organism-specific databases
CYGD	YPL242c.
SGD	S000006163. IQG1.
Phylogenomic databases
eggNOG	fuNOG04232.
GeneTree	EFGT00050000002498.
HOGENOM	HBG204004.
OMA	PSLWAVK.
OrthoDB	EOG4XH378.
Gene expression databases
ArrayExpress	Q12280.
Genevestigator	Q12280.
GermOnline	YPL242C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001715. CH-domain. IPR000048. IQ_motif_EF-hand-BS. IPR001936. RasGAP. IPR000593. RasGAP_C. IPR008936. Rho_GTPase_activation_prot. [Graphical view]
Gene3D	G3DSA:1.10.418.10. Calponin-homology. 1 hit. G3DSA:1.10.506.10. RasGAP. 1 hit.
KO	K05767.
Pfam	PF00307. CH. 1 hit. PF00612. IQ. 1 hit. PF00616. RasGAP. 1 hit. PF03836. RasGAP_C. 1 hit. [Graphical view]
SMART	SM00033. CH. 1 hit. SM00015. IQ. 3 hits. [Graphical view]
SUPFAM	SSF47576. Calponin-homology. 1 hit. SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50021. CH. 1 hit. PS50096. IQ. False negative. PS00509. RAS_GTPASE_ACTIV_1. False negative. PS50018. RAS_GTPASE_ACTIV_2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	980401.

Entry information

Entry name

IQG1_YEAST

Accession

Primary (citable) accession number: Q12280
Secondary accession number(s): D6W3C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 23, 2004
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program