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Q12272

- TPT1_YEAST

UniProt

Q12272 - TPT1_YEAST

Protein

tRNA 2'-phosphotransferase

Gene

TPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.

    Catalytic activityi

    2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1'',2''-phosphate + nicotinamide + H2O.

    GO - Molecular functioni

    1. tRNA 2'-phosphotransferase activity Source: SGD

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: SGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16153.
    YEAST:G3O-33500-MONOMER.
    BRENDAi2.7.1.160. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA 2'-phosphotransferase (EC:2.7.1.160)
    Gene namesi
    Name:TPT1
    Ordered Locus Names:YOL102C
    ORF Names:HRE230
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOL102c.
    SGDiS000005462. TPT1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230tRNA 2'-phosphotransferasePRO_0000157497Add
    BLAST

    Proteomic databases

    MaxQBiQ12272.
    PaxDbiQ12272.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12272.

    Interactioni

    Protein-protein interaction databases

    BioGridi34301. 11 interactions.
    DIPiDIP-2571N.
    IntActiQ12272. 1 interaction.
    MINTiMINT-422925.
    STRINGi4932.YOL102C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12272.
    SMRiQ12272. Positions 12-203.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the KptA/TPT1 family.Curated

    Phylogenomic databases

    eggNOGiCOG1859.
    GeneTreeiENSGT00390000002731.
    HOGENOMiHOG000229875.
    KOiK10669.
    OMAiPPDELYH.
    OrthoDBiEOG7PVX1Z.

    Family and domain databases

    InterProiIPR002745. Ptrans_KptA/Tpt1.
    [Graphical view]
    PfamiPF01885. PTS_2-RNA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12272-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRQVLQKDKR DVQLSKALSY LLRHTAVKEK LTIDSNGYTP LKELLSHNRL    50
    KTHKCTVDDI HRIVKENDKQ RFHIKTLGAD EEWICATQGH SIKSIQPSDE 100
    VLVPITEASQ LPQELIHGTN LQSVIKIIES GAISPMSRNH VHLSPGMLHA 150
    KGVISGMRSS SNVYIFIDCH SPLFFQTLKM FRSLNNVYLS SSIPVELIQK 200
    VVVKGNLKDE EKLDTLRRIL HERNIPLEKI 230
    Length:230
    Mass (Da):26,196
    Last modified:November 1, 1996 - v1
    Checksum:i7BC2136E127F076F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48149 Genomic DNA. Translation: CAA88160.1.
    Z74844 Genomic DNA. Translation: CAA99116.1.
    BK006948 Genomic DNA. Translation: DAA10682.1.
    PIRiS51897.
    RefSeqiNP_014539.1. NM_001183356.1.

    Genome annotation databases

    EnsemblFungiiYOL102C; YOL102C; YOL102C.
    GeneIDi854051.
    KEGGisce:YOL102C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z48149 Genomic DNA. Translation: CAA88160.1 .
    Z74844 Genomic DNA. Translation: CAA99116.1 .
    BK006948 Genomic DNA. Translation: DAA10682.1 .
    PIRi S51897.
    RefSeqi NP_014539.1. NM_001183356.1.

    3D structure databases

    ProteinModelPortali Q12272.
    SMRi Q12272. Positions 12-203.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34301. 11 interactions.
    DIPi DIP-2571N.
    IntActi Q12272. 1 interaction.
    MINTi MINT-422925.
    STRINGi 4932.YOL102C.

    Proteomic databases

    MaxQBi Q12272.
    PaxDbi Q12272.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOL102C ; YOL102C ; YOL102C .
    GeneIDi 854051.
    KEGGi sce:YOL102C.

    Organism-specific databases

    CYGDi YOL102c.
    SGDi S000005462. TPT1.

    Phylogenomic databases

    eggNOGi COG1859.
    GeneTreei ENSGT00390000002731.
    HOGENOMi HOG000229875.
    KOi K10669.
    OMAi PPDELYH.
    OrthoDBi EOG7PVX1Z.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16153.
    YEAST:G3O-33500-MONOMER.
    BRENDAi 2.7.1.160. 984.

    Miscellaneous databases

    NextBioi 975635.
    PROi Q12272.

    Gene expression databases

    Genevestigatori Q12272.

    Family and domain databases

    InterProi IPR002745. Ptrans_KptA/Tpt1.
    [Graphical view ]
    Pfami PF01885. PTS_2-RNA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
      Vandenbol M., Durand P., Portetelle D., Hilger F.
      Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae."
      Culver G.M., McCraith S.M., Consaul S.A., Stanford D.R., Phizicky E.M.
      J. Biol. Chem. 272:13203-13210(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast."
      Spinelli S.L., Kierzek R., Turner D.H., Phizicky E.M.
      J. Biol. Chem. 274:2637-2644(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiTPT1_YEAST
    AccessioniPrimary (citable) accession number: Q12272
    Secondary accession number(s): D6W1W6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 672 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3