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Q12272 (TPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA 2'-phosphotransferase

EC=2.7.1.160
Gene names
Name:TPT1
Ordered Locus Names:YOL102C
ORF Names:HRE230
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length230 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.

Catalytic activity

2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1'',2''-phosphate + nicotinamide + H2O.

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the KptA/TPT1 family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandNAD
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from direct assay Ref.4. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 22391451. Source: SGD

nucleus

Inferred from direct assay PubMed 22391451. Source: SGD

   Molecular_functiontRNA 2'-phosphotransferase activity

Inferred from direct assay Ref.4. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 230230tRNA 2'-phosphotransferase
PRO_0000157497

Sequences

Sequence LengthMass (Da)Tools
Q12272 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7BC2136E127F076F

FASTA23026,196
        10         20         30         40         50         60 
MRQVLQKDKR DVQLSKALSY LLRHTAVKEK LTIDSNGYTP LKELLSHNRL KTHKCTVDDI 

        70         80         90        100        110        120 
HRIVKENDKQ RFHIKTLGAD EEWICATQGH SIKSIQPSDE VLVPITEASQ LPQELIHGTN 

       130        140        150        160        170        180 
LQSVIKIIES GAISPMSRNH VHLSPGMLHA KGVISGMRSS SNVYIFIDCH SPLFFQTLKM 

       190        200        210        220        230 
FRSLNNVYLS SSIPVELIQK VVVKGNLKDE EKLDTLRRIL HERNIPLEKI 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
Vandenbol M., Durand P., Portetelle D., Hilger F.
Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae."
Culver G.M., McCraith S.M., Consaul S.A., Stanford D.R., Phizicky E.M.
J. Biol. Chem. 272:13203-13210(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast."
Spinelli S.L., Kierzek R., Turner D.H., Phizicky E.M.
J. Biol. Chem. 274:2637-2644(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z48149 Genomic DNA. Translation: CAA88160.1.
Z74844 Genomic DNA. Translation: CAA99116.1.
BK006948 Genomic DNA. Translation: DAA10682.1.
PIRS51897.
RefSeqNP_014539.1. NM_001183356.1.

3D structure databases

ProteinModelPortalQ12272.
SMRQ12272. Positions 12-203.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34301. 11 interactions.
DIPDIP-2571N.
IntActQ12272. 1 interaction.
MINTMINT-422925.
STRING4932.YOL102C.

Proteomic databases

MaxQBQ12272.
PaxDbQ12272.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOL102C; YOL102C; YOL102C.
GeneID854051.
KEGGsce:YOL102C.

Organism-specific databases

CYGDYOL102c.
SGDS000005462. TPT1.

Phylogenomic databases

eggNOGCOG1859.
GeneTreeENSGT00390000002731.
HOGENOMHOG000229875.
KOK10669.
OMAPPDELYH.
OrthoDBEOG7PVX1Z.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16153.
YEAST:G3O-33500-MONOMER.
BRENDA2.7.1.160. 984.

Gene expression databases

GenevestigatorQ12272.

Family and domain databases

InterProIPR002745. Ptrans_KptA/Tpt1.
[Graphical view]
PfamPF01885. PTS_2-RNA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio975635.
PROQ12272.

Entry information

Entry nameTPT1_YEAST
AccessionPrimary (citable) accession number: Q12272
Secondary accession number(s): D6W1W6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families