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Q12272

- TPT1_YEAST

UniProt

Q12272 - TPT1_YEAST

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Protein

tRNA 2'-phosphotransferase

Gene

TPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.

Catalytic activityi

2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1'',2''-phosphate + nicotinamide + H2O.

GO - Molecular functioni

  1. tRNA 2'-phosphotransferase activity Source: SGD

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16153.
YEAST:G3O-33500-MONOMER.
BRENDAi2.7.1.160. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 2'-phosphotransferase (EC:2.7.1.160)
Gene namesi
Name:TPT1
Ordered Locus Names:YOL102C
ORF Names:HRE230
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL102c.
SGDiS000005462. TPT1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230tRNA 2'-phosphotransferasePRO_0000157497Add
BLAST

Proteomic databases

MaxQBiQ12272.
PaxDbiQ12272.

Expressioni

Gene expression databases

GenevestigatoriQ12272.

Interactioni

Protein-protein interaction databases

BioGridi34301. 12 interactions.
DIPiDIP-2571N.
IntActiQ12272. 1 interaction.
MINTiMINT-422925.
STRINGi4932.YOL102C.

Structurei

3D structure databases

ProteinModelPortaliQ12272.
SMRiQ12272. Positions 12-203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KptA/TPT1 family.Curated

Phylogenomic databases

eggNOGiCOG1859.
GeneTreeiENSGT00390000002731.
HOGENOMiHOG000229875.
KOiK10669.
OMAiPPDELYH.
OrthoDBiEOG7PVX1Z.

Family and domain databases

InterProiIPR002745. Ptrans_KptA/Tpt1.
[Graphical view]
PfamiPF01885. PTS_2-RNA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12272 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRQVLQKDKR DVQLSKALSY LLRHTAVKEK LTIDSNGYTP LKELLSHNRL
60 70 80 90 100
KTHKCTVDDI HRIVKENDKQ RFHIKTLGAD EEWICATQGH SIKSIQPSDE
110 120 130 140 150
VLVPITEASQ LPQELIHGTN LQSVIKIIES GAISPMSRNH VHLSPGMLHA
160 170 180 190 200
KGVISGMRSS SNVYIFIDCH SPLFFQTLKM FRSLNNVYLS SSIPVELIQK
210 220 230
VVVKGNLKDE EKLDTLRRIL HERNIPLEKI
Length:230
Mass (Da):26,196
Last modified:November 1, 1996 - v1
Checksum:i7BC2136E127F076F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48149 Genomic DNA. Translation: CAA88160.1.
Z74844 Genomic DNA. Translation: CAA99116.1.
BK006948 Genomic DNA. Translation: DAA10682.1.
PIRiS51897.
RefSeqiNP_014539.1. NM_001183356.1.

Genome annotation databases

EnsemblFungiiYOL102C; YOL102C; YOL102C.
GeneIDi854051.
KEGGisce:YOL102C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48149 Genomic DNA. Translation: CAA88160.1 .
Z74844 Genomic DNA. Translation: CAA99116.1 .
BK006948 Genomic DNA. Translation: DAA10682.1 .
PIRi S51897.
RefSeqi NP_014539.1. NM_001183356.1.

3D structure databases

ProteinModelPortali Q12272.
SMRi Q12272. Positions 12-203.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34301. 12 interactions.
DIPi DIP-2571N.
IntActi Q12272. 1 interaction.
MINTi MINT-422925.
STRINGi 4932.YOL102C.

Proteomic databases

MaxQBi Q12272.
PaxDbi Q12272.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL102C ; YOL102C ; YOL102C .
GeneIDi 854051.
KEGGi sce:YOL102C.

Organism-specific databases

CYGDi YOL102c.
SGDi S000005462. TPT1.

Phylogenomic databases

eggNOGi COG1859.
GeneTreei ENSGT00390000002731.
HOGENOMi HOG000229875.
KOi K10669.
OMAi PPDELYH.
OrthoDBi EOG7PVX1Z.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16153.
YEAST:G3O-33500-MONOMER.
BRENDAi 2.7.1.160. 984.

Miscellaneous databases

NextBioi 975635.
PROi Q12272.

Gene expression databases

Genevestigatori Q12272.

Family and domain databases

InterProi IPR002745. Ptrans_KptA/Tpt1.
[Graphical view ]
Pfami PF01885. PTS_2-RNA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 44 kb DNA fragment of yeast chromosome XV including the Ty1-H3 retrotransposon, the suf1(+) frameshift suppressor gene for tRNA-Gly, the yeast transfer RNA-Thr-1a and a delta element."
    Vandenbol M., Durand P., Portetelle D., Hilger F.
    Yeast 11:1069-1075(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae."
    Culver G.M., McCraith S.M., Consaul S.A., Stanford D.R., Phizicky E.M.
    J. Biol. Chem. 272:13203-13210(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast."
    Spinelli S.L., Kierzek R., Turner D.H., Phizicky E.M.
    J. Biol. Chem. 274:2637-2644(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPT1_YEAST
AccessioniPrimary (citable) accession number: Q12272
Secondary accession number(s): D6W1W6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3