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Protein

tRNA 2'-phosphotransferase

Gene

TPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.

Miscellaneous

Present with 672 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP ribose 1'',2''-phosphate + nicotinamide + H2O.

GO - Molecular functioni

  • tRNA 2'-phosphotransferase activity Source: SGD

GO - Biological processi

  • tRNA splicing, via endonucleolytic cleavage and ligation Source: SGD

Keywordsi

Molecular functionTransferase
Biological processtRNA processing
LigandNAD

Enzyme and pathway databases

BioCyciMetaCyc:G3O-33500-MONOMER
YEAST:G3O-33500-MONOMER
BRENDAi2.7.1.160 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 2'-phosphotransferase (EC:2.7.1.160)
Gene namesi
Name:TPT1
Ordered Locus Names:YOL102C
ORF Names:HRE230
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL102C
SGDiS000005462 TPT1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001574971 – 230tRNA 2'-phosphotransferaseAdd BLAST230

Proteomic databases

MaxQBiQ12272
PaxDbiQ12272
PRIDEiQ12272

PTM databases

iPTMnetiQ12272

Interactioni

Protein-protein interaction databases

BioGridi34301, 213 interactors
DIPiDIP-2571N
IntActiQ12272, 6 interactors
MINTiQ12272
STRINGi4932.YOL102C

Structurei

3D structure databases

ProteinModelPortaliQ12272
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KptA/TPT1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000002731
HOGENOMiHOG000229875
KOiK10669
OMAiLVHGTFW
OrthoDBiEOG092C402H

Family and domain databases

InterProiView protein in InterPro
IPR002745 Ptrans_KptA/Tpt1
PANTHERiPTHR12684 PTHR12684, 1 hit
PfamiView protein in Pfam
PF01885 PTS_2-RNA, 1 hit

Sequencei

Sequence statusi: Complete.

Q12272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQVLQKDKR DVQLSKALSY LLRHTAVKEK LTIDSNGYTP LKELLSHNRL
60 70 80 90 100
KTHKCTVDDI HRIVKENDKQ RFHIKTLGAD EEWICATQGH SIKSIQPSDE
110 120 130 140 150
VLVPITEASQ LPQELIHGTN LQSVIKIIES GAISPMSRNH VHLSPGMLHA
160 170 180 190 200
KGVISGMRSS SNVYIFIDCH SPLFFQTLKM FRSLNNVYLS SSIPVELIQK
210 220 230
VVVKGNLKDE EKLDTLRRIL HERNIPLEKI
Length:230
Mass (Da):26,196
Last modified:November 1, 1996 - v1
Checksum:i7BC2136E127F076F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48149 Genomic DNA Translation: CAA88160.1
Z74844 Genomic DNA Translation: CAA99116.1
BK006948 Genomic DNA Translation: DAA10682.1
PIRiS51897
RefSeqiNP_014539.1, NM_001183356.1

Genome annotation databases

EnsemblFungiiYOL102C; YOL102C; YOL102C
GeneIDi854051
KEGGisce:YOL102C

Similar proteinsi

Entry informationi

Entry nameiTPT1_YEAST
AccessioniPrimary (citable) accession number: Q12272
Secondary accession number(s): D6W1W6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health