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Protein

Polyphosphatidylinositol phosphatase INP53

Gene

INP53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Dephosphorylates a number of phosphatidylinositols (PIs) like phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), but also phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 4-phosphate (PtdIns4P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Controls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate. Plays an essential role in a TGN (trans Golgi network)-to-early endosome pathway. Involved in clathrin-mediated protein sorting at the TGN.12 Publications

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

GO - Molecular functioni

  • phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity Source: SGD
  • phosphatidylinositol-3-phosphatase activity Source: SGD
  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: SGD
  • phosphatidylinositol-4-phosphate phosphatase activity Source: SGD

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • phosphatidylinositol dephosphorylation Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processEndocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:YOR109W-MONOMER
ReactomeiR-SCE-1660499 Synthesis of PIPs at the plasma membrane
R-SCE-1660514 Synthesis of PIPs at the Golgi membrane
R-SCE-1855183 Synthesis of IP2, IP, and Ins in the cytosol
R-SCE-1855204 Synthesis of IP3 and IP4 in the cytosol
R-SCE-194840 Rho GTPase cycle
R-SCE-202424 Downstream TCR signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphosphatidylinositol phosphatase INP53
Alternative name(s):
Suppressor of PMA1 protein 2
Synaptojanin-like protein 3
Including the following 2 domains:
SAC1-like phosphoinositide phosphatase (EC:3.1.3.-)
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase (EC:3.1.3.36)
Gene namesi
Name:INP53
Synonyms:SJL3, SOP2
Ordered Locus Names:YOR109W
ORF Names:YOR3231w
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR109W
SGDiS000005635 INP53

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi421C → A: Reduces hydrolysis of PtdIns(4)P; when associated with A-424 and A-427. 1 Publication1
Mutagenesisi424C → A: Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-427. 1 Publication1
Mutagenesisi427R → A: Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-424. 1 Publication1
Mutagenesisi746D → A: Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-748. 1 Publication1
Mutagenesisi748N → A: Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-746. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686811 – 1107Polyphosphatidylinositol phosphatase INP53Add BLAST1107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei497PhosphoserineBy similarity1
Modified residuei986PhosphoserineCombined sources1
Modified residuei1035PhosphoserineCombined sources1
Modified residuei1105PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12271
PaxDbiQ12271
PRIDEiQ12271

PTM databases

iPTMnetiQ12271

Interactioni

Subunit structurei

Interacts with BSP1 and CHC1.2 Publications

Protein-protein interaction databases

BioGridi34505, 303 interactors
DIPiDIP-2555N
IntActiQ12271, 18 interactors
MINTiQ12271
STRINGi4932.YOR109W

Structurei

3D structure databases

ProteinModelPortaliQ12271
SMRiQ12271
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 482SACPROSITE-ProRule annotationAdd BLAST341

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi957 – 989Pro-richAdd BLAST33

Domaini

The SAC1 domain is capable of hydrolyzing phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 4-phosphate (PtdIns4P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
The 5-phosphatase domain (residues 568 to 856) selectively removes the phosphate group at the 5' position of inositol of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).
The C-terminal proline-rich domain is required for the function and associates with clathrin heavy chain CHC1.

Sequence similaritiesi

Belongs to the synaptojanin family.Curated
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119075
HOGENOMiHOG000179717
InParanoidiQ12271
KOiK20279
OMAiMGIAGNK
OrthoDBiEOG092C1610

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
IPR000300 IPPc
IPR002013 SAC_dom
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
PF02383 Syja_N, 1 hit
SMARTiView protein in SMART
SM00128 IPPc, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit
PROSITEiView protein in PROSITE
PS50275 SAC, 1 hit

Sequencei

Sequence statusi: Complete.

Q12271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIFVSEEPE RRLAIVSNLY ALVLKPVGKK PSDKPLCAIE LLQKNDLKKY
60 70 80 90 100
GFKRLTSHEI FGVIGLIEVN GLLFVGAITG KSKVAQPCPG ETVNKIFAVD
110 120 130 140 150
FFCLNDNSWD FIEIDSSGYP VLPETASTEY QDALPKHPCY ELKKLLSNGS
160 170 180 190 200
FYYSSDFDLT STLQHRGYGQ HSLSTDTYEE EYMWNSFLMQ EMITYRDHLD
210 220 230 240 250
TNLKQILDDE GFLTTVIRGF AETFVSYVKK LKVALTIISK QSWKRAGTRF
260 270 280 290 300
NARGVDDEAN VANFVETEFI MYSSQYCYAF TQIRGSIPVF WEQGTSLINP
310 320 330 340 350
RVQITRSFEA TQPVFDKHIM KSVEKYGPVH VVNLLSTKSS EIELSKRYKE
360 370 380 390 400
HLTHSKKLNF NKDIFLTEFD FHKETSQEGF SGVRKLIPLI LDSLLSSGYY
410 420 430 440 450
SYDVREKKNI SEQHGIFRTN CLDCLDRTNL AQQIISLAAF RTFLEDFRLI
460 470 480 490 500
SSNSFIDDDD FVSKHNTLWA DHGDQISQIY TGTNALKSSF SRKGKMSLAG
510 520 530 540 550
ALSDATKSVS RIYINNFMDK EKQQNIDTLL GRLPYQKAVQ LYDPVNEYVS
560 570 580 590 600
TKLQSMSDKF TSTSNINLLI GSFNVNGATK KVDLSKWLFP IGEKFKPDIV
610 620 630 640 650
VLGLQEVIEL SAGSILNADY SKSSFWENLV GDCLNQYDDK YLLLRVEQMT
660 670 680 690 700
SLLILFFVKA DKAKYVKQVE GATKKTGFRG MAGNKGAVSI RFEYGATSFC
710 720 730 740 750
FVNSHLAAGA TNVEERRSDY ESIVRGITFT RTKMIPHHDS IFWLGDMNYR
760 770 780 790 800
INLPNEDVRR ELLNQEEGYI DKLLHFDQLT LGINSGSVFE GFKEPTLKFR
810 820 830 840 850
PTYKYDPGTG TYDSSEKERT PSWTDRIIYK GENLLPLSYS DAPIMISDHR
860 870 880 890 900
PVYAAYRAKI TFVDDKERLS LKKRLFTEYK QEHPEEPGSL ISDLLSLDLD
910 920 930 940 950
NKSTDGFKSS SESSLLDIDP IMAQPTASSV ASSSPVSSAS ASLQPVRTQN
960 970 980 990 1000
SSQSRTPIKK PVLRPPPPPA HKSVSAPAPS TSKEKSPTPQ TSTASLSSVT
1010 1020 1030 1040 1050
KNIQENKPLA QNRRIPPPGF SQNILTPKST SNLASPMSSK VDLYNSASES
1060 1070 1080 1090 1100
TRSAQDARQQ TPTAFAASRD VNGQPEALLG DENPIEPEEK AKLNHMTLDS

WQPLTPK
Length:1,107
Mass (Da):124,577
Last modified:November 1, 1996 - v1
Checksum:i1E024F15085261EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA Translation: CAA64029.1
Z75017 Genomic DNA Translation: CAA99307.1
BK006948 Genomic DNA Translation: DAA10884.1
PIRiS61667
RefSeqiNP_014752.3, NM_001183528.3

Genome annotation databases

EnsemblFungiiYOR109W; YOR109W; YOR109W
GeneIDi854276
KEGGisce:YOR109W

Similar proteinsi

Entry informationi

Entry nameiINP53_YEAST
AccessioniPrimary (citable) accession number: Q12271
Secondary accession number(s): D6W2G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: March 28, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health