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Protein

Polyphosphatidylinositol phosphatase INP53

Gene

INP53

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates a number of phosphatidylinositols (PIs) like phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), but also phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 4-phosphate (PtdIns4P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Controls the cellular levels and subcellular distribution of phosphatidylinositol 3-phosphate and phosphatidylinositol 4,5-bisphosphate. Plays an essential role in a TGN (trans Golgi network)-to-early endosome pathway. Involved in clathrin-mediated protein sorting at the TGN.12 Publications

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.

GO - Molecular functioni

  • phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity Source: SGD
  • phosphatidylinositol-3-phosphatase activity Source: SGD
  • phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Source: SGD
  • phosphatidylinositol-4-phosphate phosphatase activity Source: SGD

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • phosphatidylinositol dephosphorylation Source: SGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:YOR109W-MONOMER.
ReactomeiR-SCE-1660499. Synthesis of PIPs at the plasma membrane.
R-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
R-SCE-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SCE-194840. Rho GTPase cycle.
R-SCE-202424. Downstream TCR signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyphosphatidylinositol phosphatase INP53
Alternative name(s):
Suppressor of PMA1 protein 2
Synaptojanin-like protein 3
Including the following 2 domains:
SAC1-like phosphoinositide phosphatase (EC:3.1.3.-)
Phosphatidylinositol 4,5-bisphosphate 5-phosphatase (EC:3.1.3.36)
Gene namesi
Name:INP53
Synonyms:SJL3, SOP2
Ordered Locus Names:YOR109W
ORF Names:YOR3231w
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR109W.
SGDiS000005635. INP53.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • cytoplasm Source: SGD
  • membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi421C → A: Reduces hydrolysis of PtdIns(4)P; when associated with A-424 and A-427. 1 Publication1
Mutagenesisi424C → A: Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-427. 1 Publication1
Mutagenesisi427R → A: Reduces hydrolysis of PtdIns(4)P; when associated with A-421 and A-424. 1 Publication1
Mutagenesisi746D → A: Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-748. 1 Publication1
Mutagenesisi748N → A: Abolishes hydrolysis of PtdIns(4,5)P2; when associated with A-746. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002686811 – 1107Polyphosphatidylinositol phosphatase INP53Add BLAST1107

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei497PhosphoserineBy similarity1
Modified residuei986PhosphoserineCombined sources1
Modified residuei1035PhosphoserineCombined sources1
Modified residuei1105PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12271.
PRIDEiQ12271.

PTM databases

iPTMnetiQ12271.

Interactioni

Subunit structurei

Interacts with BSP1 and CHC1.2 Publications

Protein-protein interaction databases

BioGridi34505. 189 interactors.
DIPiDIP-2555N.
IntActiQ12271. 14 interactors.
MINTiMINT-4502837.

Structurei

3D structure databases

ProteinModelPortaliQ12271.
SMRiQ12271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini142 – 482SACPROSITE-ProRule annotationAdd BLAST341

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi957 – 989Pro-richAdd BLAST33

Domaini

The SAC1 domain is capable of hydrolyzing phosphatidylinositol 3-phosphate (PtdIns3P), phosphatidylinositol 4-phosphate (PtdIns4P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
The 5-phosphatase domain (residues 568 to 856) selectively removes the phosphate group at the 5' position of inositol of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2).
The C-terminal proline-rich domain is required for the function and associates with clathrin heavy chain CHC1.

Sequence similaritiesi

Belongs to the synaptojanin family.Curated
In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.Curated
Contains 1 SAC domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119075.
HOGENOMiHOG000179717.
InParanoidiQ12271.
KOiK20279.
OMAiIMGIAGN.
OrthoDBiEOG092C1610.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS50275. SAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIIFVSEEPE RRLAIVSNLY ALVLKPVGKK PSDKPLCAIE LLQKNDLKKY
60 70 80 90 100
GFKRLTSHEI FGVIGLIEVN GLLFVGAITG KSKVAQPCPG ETVNKIFAVD
110 120 130 140 150
FFCLNDNSWD FIEIDSSGYP VLPETASTEY QDALPKHPCY ELKKLLSNGS
160 170 180 190 200
FYYSSDFDLT STLQHRGYGQ HSLSTDTYEE EYMWNSFLMQ EMITYRDHLD
210 220 230 240 250
TNLKQILDDE GFLTTVIRGF AETFVSYVKK LKVALTIISK QSWKRAGTRF
260 270 280 290 300
NARGVDDEAN VANFVETEFI MYSSQYCYAF TQIRGSIPVF WEQGTSLINP
310 320 330 340 350
RVQITRSFEA TQPVFDKHIM KSVEKYGPVH VVNLLSTKSS EIELSKRYKE
360 370 380 390 400
HLTHSKKLNF NKDIFLTEFD FHKETSQEGF SGVRKLIPLI LDSLLSSGYY
410 420 430 440 450
SYDVREKKNI SEQHGIFRTN CLDCLDRTNL AQQIISLAAF RTFLEDFRLI
460 470 480 490 500
SSNSFIDDDD FVSKHNTLWA DHGDQISQIY TGTNALKSSF SRKGKMSLAG
510 520 530 540 550
ALSDATKSVS RIYINNFMDK EKQQNIDTLL GRLPYQKAVQ LYDPVNEYVS
560 570 580 590 600
TKLQSMSDKF TSTSNINLLI GSFNVNGATK KVDLSKWLFP IGEKFKPDIV
610 620 630 640 650
VLGLQEVIEL SAGSILNADY SKSSFWENLV GDCLNQYDDK YLLLRVEQMT
660 670 680 690 700
SLLILFFVKA DKAKYVKQVE GATKKTGFRG MAGNKGAVSI RFEYGATSFC
710 720 730 740 750
FVNSHLAAGA TNVEERRSDY ESIVRGITFT RTKMIPHHDS IFWLGDMNYR
760 770 780 790 800
INLPNEDVRR ELLNQEEGYI DKLLHFDQLT LGINSGSVFE GFKEPTLKFR
810 820 830 840 850
PTYKYDPGTG TYDSSEKERT PSWTDRIIYK GENLLPLSYS DAPIMISDHR
860 870 880 890 900
PVYAAYRAKI TFVDDKERLS LKKRLFTEYK QEHPEEPGSL ISDLLSLDLD
910 920 930 940 950
NKSTDGFKSS SESSLLDIDP IMAQPTASSV ASSSPVSSAS ASLQPVRTQN
960 970 980 990 1000
SSQSRTPIKK PVLRPPPPPA HKSVSAPAPS TSKEKSPTPQ TSTASLSSVT
1010 1020 1030 1040 1050
KNIQENKPLA QNRRIPPPGF SQNILTPKST SNLASPMSSK VDLYNSASES
1060 1070 1080 1090 1100
TRSAQDARQQ TPTAFAASRD VNGQPEALLG DENPIEPEEK AKLNHMTLDS

WQPLTPK
Length:1,107
Mass (Da):124,577
Last modified:November 1, 1996 - v1
Checksum:i1E024F15085261EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64029.1.
Z75017 Genomic DNA. Translation: CAA99307.1.
BK006948 Genomic DNA. Translation: DAA10884.1.
PIRiS61667.
RefSeqiNP_014752.3. NM_001183528.3.

Genome annotation databases

EnsemblFungiiYOR109W; YOR109W; YOR109W.
GeneIDi854276.
KEGGisce:YOR109W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94335 Genomic DNA. Translation: CAA64029.1.
Z75017 Genomic DNA. Translation: CAA99307.1.
BK006948 Genomic DNA. Translation: DAA10884.1.
PIRiS61667.
RefSeqiNP_014752.3. NM_001183528.3.

3D structure databases

ProteinModelPortaliQ12271.
SMRiQ12271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34505. 189 interactors.
DIPiDIP-2555N.
IntActiQ12271. 14 interactors.
MINTiMINT-4502837.

PTM databases

iPTMnetiQ12271.

Proteomic databases

MaxQBiQ12271.
PRIDEiQ12271.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR109W; YOR109W; YOR109W.
GeneIDi854276.
KEGGisce:YOR109W.

Organism-specific databases

EuPathDBiFungiDB:YOR109W.
SGDiS000005635. INP53.

Phylogenomic databases

GeneTreeiENSGT00760000119075.
HOGENOMiHOG000179717.
InParanoidiQ12271.
KOiK20279.
OMAiIMGIAGN.
OrthoDBiEOG092C1610.

Enzyme and pathway databases

BioCyciYEAST:YOR109W-MONOMER.
ReactomeiR-SCE-1660499. Synthesis of PIPs at the plasma membrane.
R-SCE-1660514. Synthesis of PIPs at the Golgi membrane.
R-SCE-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
R-SCE-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-SCE-194840. Rho GTPase cycle.
R-SCE-202424. Downstream TCR signaling.

Miscellaneous databases

PROiQ12271.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR000300. IPPc.
IPR002013. SAC_dom.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF02383. Syja_N. 1 hit.
[Graphical view]
SMARTiSM00128. IPPc. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
PROSITEiPS50275. SAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiINP53_YEAST
AccessioniPrimary (citable) accession number: Q12271
Secondary accession number(s): D6W2G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.