SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q12270

- RBD2_YEAST

UniProt

Q12270 - RBD2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Rhomboid protein 2
Gene
RBD2, YPL246C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Probable serine protease By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241Nucleophile By similarity
Active sitei179 – 1791 By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciYEAST:G3O-34132-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhomboid protein 2 (EC:3.4.21.-)
Gene namesi
Name:RBD2
Ordered Locus Names:YPL246C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL246c.
SGDiS000006167. RBD2.

Subcellular locationi

Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatuscis-Golgi network membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei17 – 3721Helical; Reviewed prediction
Add
BLAST
Topological domaini38 – 5720Lumenal Reviewed prediction
Add
BLAST
Transmembranei58 – 7821Helical; Reviewed prediction
Add
BLAST
Topological domaini79 – 8911Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei90 – 11021Helical; Reviewed prediction
Add
BLAST
Topological domaini111 – 1122Lumenal Reviewed prediction
Transmembranei113 – 13321Helical; Reviewed prediction
Add
BLAST
Topological domaini134 – 15118Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei152 – 16817Helical; Reviewed prediction
Add
BLAST
Topological domaini169 – 1746Lumenal Reviewed prediction
Transmembranei175 – 19117Helical; Reviewed prediction
Add
BLAST
Topological domaini192 – 26271Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Rhomboid protein 2
PRO_0000206193Add
BLAST

Proteomic databases

MaxQBiQ12270.
PaxDbiQ12270.

Expressioni

Gene expression databases

GenevestigatoriQ12270.

Interactioni

Subunit structurei

Interacts with SNX3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VAM7P329123EBI-31471,EBI-20232

Protein-protein interaction databases

BioGridi35917. 79 interactions.
DIPiDIP-1734N.
IntActiQ12270. 47 interactions.
MINTiMINT-408864.
STRINGi4932.YPL246C.

Structurei

3D structure databases

ProteinModelPortaliQ12270.
SMRiQ12270. Positions 15-192.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0705.
HOGENOMiHOG000111157.
KOiK01362.
OMAiWEISTEH.
OrthoDBiEOG7Q8CZT.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12270-1 [UniParc]FASTAAdd to Basket

« Hide

MNWKSYVFPG GHPPAALTTG LVVFLTAIYL LSFIFALRED LSLAPESLFK    50
LQMSRLSLYP LIHLSLPHLL FNVLAIWAPL NLFEETHGTV YTGVFLNLSA 100
LFAGILYCLL GKLLYPEALV AGASGWCFTL FAYYSFKESQ IRPRTRIFRT 150
DYSIPTLYTP LVLLVAIAVV IPGSSFWGHF FGLCVGYAIG YKESWFNKIT 200
PPGWIITKIE KSLDGLIRLI PWGIKYYRDE DIDRTKDYEP LMSTETPLPL 250
HNDNSGTVLG TA 262
Length:262
Mass (Da):29,500
Last modified:November 1, 1996 - v1
Checksum:i3A436E3FEAB7FD35
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73602 Genomic DNA. Translation: CAA97967.1.
Z67751 Genomic DNA. Translation: CAA91598.1.
BK006949 Genomic DNA. Translation: DAA11191.1.
PIRiS61018.
RefSeqiNP_015078.1. NM_001184060.1.

Genome annotation databases

EnsemblFungiiYPL246C; YPL246C; YPL246C.
GeneIDi855830.
KEGGisce:YPL246C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73602 Genomic DNA. Translation: CAA97967.1 .
Z67751 Genomic DNA. Translation: CAA91598.1 .
BK006949 Genomic DNA. Translation: DAA11191.1 .
PIRi S61018.
RefSeqi NP_015078.1. NM_001184060.1.

3D structure databases

ProteinModelPortali Q12270.
SMRi Q12270. Positions 15-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35917. 79 interactions.
DIPi DIP-1734N.
IntActi Q12270. 47 interactions.
MINTi MINT-408864.
STRINGi 4932.YPL246C.

Proteomic databases

MaxQBi Q12270.
PaxDbi Q12270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL246C ; YPL246C ; YPL246C .
GeneIDi 855830.
KEGGi sce:YPL246C.

Organism-specific databases

CYGDi YPL246c.
SGDi S000006167. RBD2.

Phylogenomic databases

eggNOGi COG0705.
HOGENOMi HOG000111157.
KOi K01362.
OMAi WEISTEH.
OrthoDBi EOG7Q8CZT.

Enzyme and pathway databases

BioCyci YEAST:G3O-34132-MONOMER.

Miscellaneous databases

NextBioi 980389.

Gene expression databases

Genevestigatori Q12270.

Family and domain databases

Gene3Di 1.20.1540.10. 1 hit.
InterProi IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view ]
Pfami PF01694. Rhomboid. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Mitochondrial membrane remodelling regulated by a conserved rhomboid protease."
    McQuibban G.A., Saurya S., Freeman M.
    Nature 423:537-541(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The phox homology (PX) domain protein interaction network in yeast."
    Vollert C.S., Uetz P.
    Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX3.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiRBD2_YEAST
AccessioniPrimary (citable) accession number: Q12270
Secondary accession number(s): D6W3C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7850 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi