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Q12270

- RBD2_YEAST

UniProt

Q12270 - RBD2_YEAST

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Protein

Rhomboid protein 2

Gene

RBD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Probable serine protease.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241NucleophileBy similarity
Active sitei179 – 1791By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciYEAST:G3O-34132-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhomboid protein 2 (EC:3.4.21.-)
Gene namesi
Name:RBD2
Ordered Locus Names:YPL246C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPL246c.
SGDiS000006167. RBD2.

Subcellular locationi

Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication. Golgi apparatuscis-Golgi network membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 262262Rhomboid protein 2PRO_0000206193Add
BLAST

Proteomic databases

MaxQBiQ12270.
PaxDbiQ12270.

Expressioni

Gene expression databases

GenevestigatoriQ12270.

Interactioni

Subunit structurei

Interacts with SNX3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VAM7P329123EBI-31471,EBI-20232

Protein-protein interaction databases

BioGridi35917. 79 interactions.
DIPiDIP-1734N.
IntActiQ12270. 47 interactions.
MINTiMINT-408864.
STRINGi4932.YPL246C.

Structurei

3D structure databases

ProteinModelPortaliQ12270.
SMRiQ12270. Positions 15-192.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini38 – 5720LumenalSequence AnalysisAdd
BLAST
Topological domaini79 – 8911CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini111 – 1122LumenalSequence Analysis
Topological domaini134 – 15118CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini169 – 1746LumenalSequence Analysis
Topological domaini192 – 26271CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei17 – 3721HelicalSequence AnalysisAdd
BLAST
Transmembranei58 – 7821HelicalSequence AnalysisAdd
BLAST
Transmembranei90 – 11021HelicalSequence AnalysisAdd
BLAST
Transmembranei113 – 13321HelicalSequence AnalysisAdd
BLAST
Transmembranei152 – 16817HelicalSequence AnalysisAdd
BLAST
Transmembranei175 – 19117HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0705.
HOGENOMiHOG000111157.
InParanoidiQ12270.
KOiK01362.
OMAiWEISTEH.
OrthoDBiEOG7Q8CZT.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12270-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNWKSYVFPG GHPPAALTTG LVVFLTAIYL LSFIFALRED LSLAPESLFK
60 70 80 90 100
LQMSRLSLYP LIHLSLPHLL FNVLAIWAPL NLFEETHGTV YTGVFLNLSA
110 120 130 140 150
LFAGILYCLL GKLLYPEALV AGASGWCFTL FAYYSFKESQ IRPRTRIFRT
160 170 180 190 200
DYSIPTLYTP LVLLVAIAVV IPGSSFWGHF FGLCVGYAIG YKESWFNKIT
210 220 230 240 250
PPGWIITKIE KSLDGLIRLI PWGIKYYRDE DIDRTKDYEP LMSTETPLPL
260
HNDNSGTVLG TA
Length:262
Mass (Da):29,500
Last modified:November 1, 1996 - v1
Checksum:i3A436E3FEAB7FD35
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73602 Genomic DNA. Translation: CAA97967.1.
Z67751 Genomic DNA. Translation: CAA91598.1.
BK006949 Genomic DNA. Translation: DAA11191.1.
PIRiS61018.
RefSeqiNP_015078.1. NM_001184060.1.

Genome annotation databases

EnsemblFungiiYPL246C; YPL246C; YPL246C.
GeneIDi855830.
KEGGisce:YPL246C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z73602 Genomic DNA. Translation: CAA97967.1 .
Z67751 Genomic DNA. Translation: CAA91598.1 .
BK006949 Genomic DNA. Translation: DAA11191.1 .
PIRi S61018.
RefSeqi NP_015078.1. NM_001184060.1.

3D structure databases

ProteinModelPortali Q12270.
SMRi Q12270. Positions 15-192.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35917. 79 interactions.
DIPi DIP-1734N.
IntActi Q12270. 47 interactions.
MINTi MINT-408864.
STRINGi 4932.YPL246C.

Proteomic databases

MaxQBi Q12270.
PaxDbi Q12270.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPL246C ; YPL246C ; YPL246C .
GeneIDi 855830.
KEGGi sce:YPL246C.

Organism-specific databases

CYGDi YPL246c.
SGDi S000006167. RBD2.

Phylogenomic databases

eggNOGi COG0705.
HOGENOMi HOG000111157.
InParanoidi Q12270.
KOi K01362.
OMAi WEISTEH.
OrthoDBi EOG7Q8CZT.

Enzyme and pathway databases

BioCyci YEAST:G3O-34132-MONOMER.

Miscellaneous databases

NextBioi 980389.

Gene expression databases

Genevestigatori Q12270.

Family and domain databases

Gene3Di 1.20.1540.10. 1 hit.
InterProi IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view ]
Pfami PF01694. Rhomboid. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Mitochondrial membrane remodelling regulated by a conserved rhomboid protease."
    McQuibban G.A., Saurya S., Freeman M.
    Nature 423:537-541(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The phox homology (PX) domain protein interaction network in yeast."
    Vollert C.S., Uetz P.
    Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SNX3.
  7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiRBD2_YEAST
AccessioniPrimary (citable) accession number: Q12270
Secondary accession number(s): D6W3C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7850 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3