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Q12270 (RBD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Rhomboid protein 2

EC=3.4.21.-
Gene names
Name:RBD2
Ordered Locus Names:YPL246C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable serine protease By similarity.

Subunit structure

Interacts with SNX3. Ref.6

Subcellular location

Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatuscis-Golgi network membrane; Multi-pass membrane protein Ref.4.

Miscellaneous

Present with 7850 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase S54 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VAM7P329123EBI-31471,EBI-20232

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Rhomboid protein 2
PRO_0000206193

Regions

Topological domain1 – 1616Cytoplasmic Potential
Transmembrane17 – 3721Helical; Potential
Topological domain38 – 5720Lumenal Potential
Transmembrane58 – 7821Helical; Potential
Topological domain79 – 8911Cytoplasmic Potential
Transmembrane90 – 11021Helical; Potential
Topological domain111 – 1122Lumenal Potential
Transmembrane113 – 13321Helical; Potential
Topological domain134 – 15118Cytoplasmic Potential
Transmembrane152 – 16817Helical; Potential
Topological domain169 – 1746Lumenal Potential
Transmembrane175 – 19117Helical; Potential
Topological domain192 – 26271Cytoplasmic Potential

Sites

Active site1241Nucleophile By similarity
Active site1791 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q12270 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3A436E3FEAB7FD35

FASTA26229,500
        10         20         30         40         50         60 
MNWKSYVFPG GHPPAALTTG LVVFLTAIYL LSFIFALRED LSLAPESLFK LQMSRLSLYP 

        70         80         90        100        110        120 
LIHLSLPHLL FNVLAIWAPL NLFEETHGTV YTGVFLNLSA LFAGILYCLL GKLLYPEALV 

       130        140        150        160        170        180 
AGASGWCFTL FAYYSFKESQ IRPRTRIFRT DYSIPTLYTP LVLLVAIAVV IPGSSFWGHF 

       190        200        210        220        230        240 
FGLCVGYAIG YKESWFNKIT PPGWIITKIE KSLDGLIRLI PWGIKYYRDE DIDRTKDYEP 

       250        260 
LMSTETPLPL HNDNSGTVLG TA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Mitochondrial membrane remodelling regulated by a conserved rhomboid protease."
McQuibban G.A., Saurya S., Freeman M.
Nature 423:537-541(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The phox homology (PX) domain protein interaction network in yeast."
Vollert C.S., Uetz P.
Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SNX3.
[7]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z73602 Genomic DNA. Translation: CAA97967.1.
Z67751 Genomic DNA. Translation: CAA91598.1.
BK006949 Genomic DNA. Translation: DAA11191.1.
PIRS61018.
RefSeqNP_015078.1. NM_001184060.1.

3D structure databases

ProteinModelPortalQ12270.
SMRQ12270. Positions 15-192.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35917. 79 interactions.
DIPDIP-1734N.
IntActQ12270. 47 interactions.
MINTMINT-408864.
STRING4932.YPL246C.

Proteomic databases

MaxQBQ12270.
PaxDbQ12270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPL246C; YPL246C; YPL246C.
GeneID855830.
KEGGsce:YPL246C.

Organism-specific databases

CYGDYPL246c.
SGDS000006167. RBD2.

Phylogenomic databases

eggNOGCOG0705.
HOGENOMHOG000111157.
KOK01362.
OMAWEISTEH.
OrthoDBEOG7Q8CZT.

Enzyme and pathway databases

BioCycYEAST:G3O-34132-MONOMER.

Gene expression databases

GenevestigatorQ12270.

Family and domain databases

Gene3D1.20.1540.10. 1 hit.
InterProIPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PfamPF01694. Rhomboid. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio980389.

Entry information

Entry nameRBD2_YEAST
AccessionPrimary (citable) accession number: Q12270
Secondary accession number(s): D6W3C5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries