Q12270 (RBD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhomboid protein 2
EC=3.4.21.-

Gene names

Name:	RBD2
Ordered Locus Names:	YPL246C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	262 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable serine protease By similarity.
Subunit structure	Interacts with SNX3. Ref.6
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Golgi apparatus › cis-Golgi network membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 7850 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase S54 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay Ref.4. Source: SGD Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VAM7	P32912	3	EBI-31471,EBI-20232

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 262	262	Rhomboid protein 2		PRO_0000206193
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Potential
	Topological domain	38 – 57	20	Lumenal Potential
	Transmembrane	58 – 78	21	Helical; Potential
	Topological domain	79 – 89	11	Cytoplasmic Potential
	Transmembrane	90 – 110	21	Helical; Potential
	Topological domain	111 – 112	2	Lumenal Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Topological domain	134 – 151	18	Cytoplasmic Potential
	Transmembrane	152 – 168	17	Helical; Potential
	Topological domain	169 – 174	6	Lumenal Potential
	Transmembrane	175 – 191	17	Helical; Potential
	Topological domain	192 – 262	71	Cytoplasmic Potential
Sites
	Active site	124	1	Nucleophile By similarity
	Active site	179	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q12270 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3A436E3FEAB7FD35	FASTA	262	29,500
	10 20 30 40 50 60 MNWKSYVFPG GHPPAALTTG LVVFLTAIYL LSFIFALRED LSLAPESLFK LQMSRLSLYP 70 80 90 100 110 120 LIHLSLPHLL FNVLAIWAPL NLFEETHGTV YTGVFLNLSA LFAGILYCLL GKLLYPEALV 130 140 150 160 170 180 AGASGWCFTL FAYYSFKESQ IRPRTRIFRT DYSIPTLYTP LVLLVAIAVV IPGSSFWGHF 190 200 210 220 230 240 FGLCVGYAIG YKESWFNKIT PPGWIITKIE KSLDGLIRLI PWGIKYYRDE DIDRTKDYEP 250 260 LMSTETPLPL HNDNSGTVLG TA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Mitochondrial membrane remodelling regulated by a conserved rhomboid protease." McQuibban G.A., Saurya S., Freeman M. Nature 423:537-541(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GENE NAME.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The phox homology (PX) domain protein interaction network in yeast." Vollert C.S., Uetz P. Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SNX3.
[7]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73602 Genomic DNA. Translation: CAA97967.1. Z67751 Genomic DNA. Translation: CAA91598.1. BK006949 Genomic DNA. Translation: DAA11191.1.
PIR	S61018.
RefSeq	NP_015078.1. NM_001184060.1.
3D structure databases
ProteinModelPortal	Q12270.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1734N.
IntAct	Q12270. 47 interactions.
MINT	MINT-408864.
STRING	4932.YPL246C.
Proteomic databases
PaxDb	Q12270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL246C; YPL246C; YPL246C.
GeneID	855830.
KEGG	sce:YPL246C.
Organism-specific databases
CYGD	YPL246c.
SGD	S000006167. RBD2.
Phylogenomic databases
eggNOG	COG0705.
HOGENOM	HOG000111157.
KO	K01362.
OMA	PHYVSVD.
OrthoDB	EOG45XC5M.
Gene expression databases
Genevestigator	Q12270.
GermOnline	YPL246C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022764. Peptidase_S54_rhomboid_dom. [Graphical view]
Pfam	PF01694. Rhomboid. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980389.

Entry information

Entry name

RBD2_YEAST

Accession

Primary (citable) accession number: Q12270
Secondary accession number(s): D6W3C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program