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Q12270

- RBD2_YEAST

UniProt

Q12270 - RBD2_YEAST

Protein

Rhomboid protein 2

Gene

RBD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Probable serine protease.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei124 – 1241NucleophileBy similarity
    Active sitei179 – 1791By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34132-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rhomboid protein 2 (EC:3.4.21.-)
    Gene namesi
    Name:RBD2
    Ordered Locus Names:YPL246C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPL246c.
    SGDiS000006167. RBD2.

    Subcellular locationi

    Golgi apparatus membrane 1 Publication; Multi-pass membrane protein 1 Publication. Golgi apparatuscis-Golgi network membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 262262Rhomboid protein 2PRO_0000206193Add
    BLAST

    Proteomic databases

    MaxQBiQ12270.
    PaxDbiQ12270.

    Expressioni

    Gene expression databases

    GenevestigatoriQ12270.

    Interactioni

    Subunit structurei

    Interacts with SNX3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VAM7P329123EBI-31471,EBI-20232

    Protein-protein interaction databases

    BioGridi35917. 79 interactions.
    DIPiDIP-1734N.
    IntActiQ12270. 47 interactions.
    MINTiMINT-408864.
    STRINGi4932.YPL246C.

    Structurei

    3D structure databases

    ProteinModelPortaliQ12270.
    SMRiQ12270. Positions 15-192.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1616CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini38 – 5720LumenalSequence AnalysisAdd
    BLAST
    Topological domaini79 – 8911CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini111 – 1122LumenalSequence Analysis
    Topological domaini134 – 15118CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini169 – 1746LumenalSequence Analysis
    Topological domaini192 – 26271CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei58 – 7821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei90 – 11021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei152 – 16817HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19117HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S54 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0705.
    HOGENOMiHOG000111157.
    KOiK01362.
    OMAiWEISTEH.
    OrthoDBiEOG7Q8CZT.

    Family and domain databases

    Gene3Di1.20.1540.10. 1 hit.
    InterProiIPR022764. Peptidase_S54_rhomboid_dom.
    [Graphical view]
    PfamiPF01694. Rhomboid. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q12270-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNWKSYVFPG GHPPAALTTG LVVFLTAIYL LSFIFALRED LSLAPESLFK    50
    LQMSRLSLYP LIHLSLPHLL FNVLAIWAPL NLFEETHGTV YTGVFLNLSA 100
    LFAGILYCLL GKLLYPEALV AGASGWCFTL FAYYSFKESQ IRPRTRIFRT 150
    DYSIPTLYTP LVLLVAIAVV IPGSSFWGHF FGLCVGYAIG YKESWFNKIT 200
    PPGWIITKIE KSLDGLIRLI PWGIKYYRDE DIDRTKDYEP LMSTETPLPL 250
    HNDNSGTVLG TA 262
    Length:262
    Mass (Da):29,500
    Last modified:November 1, 1996 - v1
    Checksum:i3A436E3FEAB7FD35
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73602 Genomic DNA. Translation: CAA97967.1.
    Z67751 Genomic DNA. Translation: CAA91598.1.
    BK006949 Genomic DNA. Translation: DAA11191.1.
    PIRiS61018.
    RefSeqiNP_015078.1. NM_001184060.1.

    Genome annotation databases

    EnsemblFungiiYPL246C; YPL246C; YPL246C.
    GeneIDi855830.
    KEGGisce:YPL246C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73602 Genomic DNA. Translation: CAA97967.1 .
    Z67751 Genomic DNA. Translation: CAA91598.1 .
    BK006949 Genomic DNA. Translation: DAA11191.1 .
    PIRi S61018.
    RefSeqi NP_015078.1. NM_001184060.1.

    3D structure databases

    ProteinModelPortali Q12270.
    SMRi Q12270. Positions 15-192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35917. 79 interactions.
    DIPi DIP-1734N.
    IntActi Q12270. 47 interactions.
    MINTi MINT-408864.
    STRINGi 4932.YPL246C.

    Proteomic databases

    MaxQBi Q12270.
    PaxDbi Q12270.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL246C ; YPL246C ; YPL246C .
    GeneIDi 855830.
    KEGGi sce:YPL246C.

    Organism-specific databases

    CYGDi YPL246c.
    SGDi S000006167. RBD2.

    Phylogenomic databases

    eggNOGi COG0705.
    HOGENOMi HOG000111157.
    KOi K01362.
    OMAi WEISTEH.
    OrthoDBi EOG7Q8CZT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34132-MONOMER.

    Miscellaneous databases

    NextBioi 980389.

    Gene expression databases

    Genevestigatori Q12270.

    Family and domain databases

    Gene3Di 1.20.1540.10. 1 hit.
    InterProi IPR022764. Peptidase_S54_rhomboid_dom.
    [Graphical view ]
    Pfami PF01694. Rhomboid. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Mitochondrial membrane remodelling regulated by a conserved rhomboid protease."
      McQuibban G.A., Saurya S., Freeman M.
      Nature 423:537-541(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The phox homology (PX) domain protein interaction network in yeast."
      Vollert C.S., Uetz P.
      Mol. Cell. Proteomics 3:1053-1064(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX3.
    7. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiRBD2_YEAST
    AccessioniPrimary (citable) accession number: Q12270
    Secondary accession number(s): D6W3C5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7850 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3