Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribose-phosphate pyrophosphokinase 5

Gene

PRS5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

5-phosphoribose 1-diphosphate synthase involved in nucleotide, histidine, and tryptophan biosynthesis. Active in heteromultimeric complexes with other 5-phosphoribose 1-diphosphate synthases (PRS2, PRS3, PRS4 and PRS5).3 Publications

Miscellaneous

Present with 3310 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.3 Publications

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase 2 (PRS2), Ribose-phosphate pyrophosphokinase 4 (PRS4), Ribose-phosphate pyrophosphokinase 1 (PRS1), Ribose-phosphate pyrophosphokinase 5 (PRS5), Ribose-phosphate pyrophosphokinase 3 (PRS3)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi242MagnesiumSequence analysis1
Metal bindingi244MagnesiumSequence analysis1
Metal bindingi253MagnesiumSequence analysis1
Metal bindingi257MagnesiumSequence analysis1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • magnesium ion binding Source: InterPro
  • ribose phosphate diphosphokinase activity Source: SGD

GO - Biological processi

  • 5-phosphoribose 1-diphosphate biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: SGD
  • nucleoside metabolic process Source: InterPro
  • nucleotide biosynthetic process Source: UniProtKB-KW
  • ribonucleoside monophosphate biosynthetic process Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOL061W-MONOMER
UniPathwayiUPA00087; UER00172

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinase 5 (EC:2.7.6.1)
Alternative name(s):
Phosphoribosyl pyrophosphate synthase 5
Gene namesi
Name:PRS5
Synonyms:PRPS5
Ordered Locus Names:YOL061W
ORF Names:O1213
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL061W
SGDiS000005422 PRS5

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410901 – 496Ribose-phosphate pyrophosphokinase 5Add BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei119PhosphotyrosineCombined sources1
Modified residuei120PhosphothreonineCombined sources1
Modified residuei123PhosphoserineCombined sources1
Modified residuei127PhosphothreonineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12265
PaxDbiQ12265
PRIDEiQ12265

PTM databases

iPTMnetiQ12265

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34340, 180 interactors
DIPiDIP-1532N
IntActiQ12265, 10 interactors
MINTiQ12265
STRINGi4932.YOL061W

Structurei

3D structure databases

ProteinModelPortaliQ12265
SMRiQ12265
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000076044
HOGENOMiHOG000210451
InParanoidiQ12265
KOiK00948
OMAiNDESTHV
OrthoDBiEOG092C2PL8

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR000842 PRib_PP_synth_CS
IPR029099 Pribosyltran_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005946 Rib-P_diPkinase
PfamiView protein in Pfam
PF14572 Pribosyl_synth, 1 hit
PF13793 Pribosyltran_N, 1 hit
SUPFAMiSSF53271 SSF53271, 4 hits
PROSITEiView protein in PROSITE
PS00114 PRPP_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q12265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMSNIVVFG GDSHPELVTK ICENLDIHPS KVELGKFSNG ETNIALRESV
60 70 80 90 100
REKDVYIIQS GCGQVNDTFM QLLILISACK SASASRVTAV MPYLCYSRQP
110 120 130 140 150
DIPYTAKGAP IISKPKENYT FESHPGTPVS SSLMTQRPGA ESSLKSLDSA
160 170 180 190 200
IRSTINLENP QPIRTPNSSA TANNNFDIKK TLSFSRIPMI PGGKLQNTSN
210 220 230 240 250
STDAGELFNA QNAGYKLWVV QAGTLIAHLL SAAGADHVIT MDLHDPQFPG
260 270 280 290 300
FFDIPVDNLY CKPIAQNYIQ HRIPDYQDAV IVSPDAGGAK RATAIADALE
310 320 330 340 350
LSFALIHKER RSQLLKGPPD ATLTSGGSLP VSPRPLVTTL VSSQNTTSSG
360 370 380 390 400
ATGVAALEMK KTTSTSSTSS QSSNSSKFVQ TTMLVGDVRN KVCIIVDDLV
410 420 430 440 450
DTSYTITRAA KLLKDQGSTK VYALITHGVF SGDALERIGQ SSIDKLIISN
460 470 480 490
TVPQDRTLQY LGKDRVDVID VSCIIGEAIR RIHNGESISM LFEHGW
Length:496
Mass (Da):53,505
Last modified:November 1, 1996 - v1
Checksum:i75C948D40D58256D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z74803 Genomic DNA Translation: CAA99070.1
X91067 Genomic DNA Translation: CAA62523.1
BK006948 Genomic DNA Translation: DAA10722.1
PIRiS61716
RefSeqiNP_014580.1, NM_001183316.1

Genome annotation databases

EnsemblFungiiYOL061W; YOL061W; YOL061W
GeneIDi854093
KEGGisce:YOL061W

Similar proteinsi

Entry informationi

Entry nameiKPR5_YEAST
AccessioniPrimary (citable) accession number: Q12265
Secondary accession number(s): D6W206
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health