Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q12262 (MCM16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Central kinetochore subunit MCM16
Alternative name(s):
Minichromosome maintenance protein 16
Gene names
Name:MCM16
Ordered Locus Names:YPR046W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Ref.3

Subunit structure

Heterotrimer. CTF3, MCM16 and MCM22 form the CTF3 kinetochore subcomplex. The CTF3 subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CTF19. Ref.5

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Associated with kinetochores. Ref.5 Ref.6

Miscellaneous

Present with 279 molecules/cell in log phase SD medium. Ref.7

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Central kinetochore subunit MCM16
PRO_0000096281

Regions

Coiled coil112 – 17160 Potential

Sequences

Sequence LengthMass (Da)Tools
Q12262 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 73E00DC745D54FF0

FASTA18121,138
        10         20         30         40         50         60 
MTNSSEKQWE RIQQLEKEHV EVYRELLITL DRLYLIRKHN HAVILSHTQQ RLLEIRHQLQ 

        70         80         90        100        110        120 
INLEKTALLI RLLEKPDNTN VLFTKLQNLL EESNSLDYEL LQSLGAQSSL HKQLIESRAE 

       130        140        150        160        170        180 
RDELMSKLIE LSSKFPKPTI PPDDSDTAGK QVEVEKENET IQELMIALQI HSGYTNISYT 


I 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"The MCM16 gene of the yeast Saccharomyces cerevisiae is required for chromosome segregation."
Sanyal K., Ghosh S.K., Sinha P.
Mol. Gen. Genet. 260:242-250(1998) [PubMed: 9862478] [Abstract]
Cited for: FUNCTION.
[4]"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
Cell 111:163-172(2002) [PubMed: 12408861] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
[5]"Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore."
Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P.
Genes Dev. 16:101-113(2002) [PubMed: 11782448] [Abstract]
Cited for: INTERACTION WITH CTF3; CTF19 AND MCM22, SUBCELLULAR LOCATION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49219 Genomic DNA. Translation: CAA89166.1.
Z71255 Genomic DNA. Translation: CAA94993.1.
BK006949 Genomic DNA. Translation: DAA11470.1.
PIRS54070.
RefSeqNP_015371.1. NM_001184143.1.

3D structure databases

ProteinModelPortalQ12262.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1430N.
IntActQ12262. 20 interactions.
MINTMINT-393701.
STRINGQ12262.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR046W; YPR046W; YPR046W.
GeneID856159.
KEGGsce:YPR046W.
NMPDRfig|4932.3.peg.6507.

Organism-specific databases

CYGDYPR046w.
SGDS000006250. MCM16.

Phylogenomic databases

eggNOGfuNOG12885.
OMACALEREH.
OrthoDBEOG4K9FP3.

Gene expression databases

ArrayExpressQ12262.
GenevestigatorQ12262.
GermOnlineYPR046W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNetSearch...

Other

NextBio981298.

Entry information

Entry nameMCM16_YEAST
AccessionPrimary (citable) accession number: Q12262
Secondary accession number(s): D6W454
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1996
Last modified: December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD