Reviewed, UniProtKB/Swiss-Prot Q12262 (MCM16_YEAST)

Names and origin

Protein names

Recommended name:
    Central kinetochore subunit MCM16
Alternative name(s):
    Minichromosome maintenance protein 16

Gene names

Name:	MCM16
Ordered Locus Names:	YPR046W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Ref.2
Subunit structure	Heterotrimer. CTF3, MCM16 and MCM22 form the CTF3 kinetochore subcomplex. The CTF3 subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CTF19. Ref.4
Subcellular location	Nucleus. Kinetochore. Note: Associated with kinetochores. Ref.4 Ref.5
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Kinetochore Nucleus
Domain	Coiled coil
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Ref.4 Inferred from physical interaction. Source: SGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	condensed nuclear chromosome kinetochore Ref.4 Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTF19	Q02732	1	EBI-31487,EBI-5199
CTF3	Q12748	1	EBI-31487,EBI-30457
FAR7	P43592	1	EBI-31487,EBI-22932
MCM22	P47167	4	EBI-31487,EBI-25691

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Central kinetochore subunit MCM16		PRO_0000096281
Regions
	Coiled coil	112 – 171	60	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12262-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 73E00DC745D54FF0	FASTA	181	21,138
	10 20 30 40 50 60 MTNSSEKQWE RIQQLEKEHV EVYRELLITL DRLYLIRKHN HAVILSHTQQ RLLEIRHQLQ 70 80 90 100 110 120 INLEKTALLI RLLEKPDNTN VLFTKLQNLL EESNSLDYEL LQSLGAQSSL HKQLIESRAE 130 140 150 160 170 180 RDELMSKLIE LSSKFPKPTI PPDDSDTAGK QVEVEKENET IQELMIALQI HSGYTNISYT I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The MCM16 gene of the yeast Saccharomyces cerevisiae is required for chromosome segregation." Sanyal K., Ghosh S.K., Sinha P. Mol. Gen. Genet. 260:242-250(1998) [PubMed: 9862478] [Abstract] Cited for: FUNCTION.
[3]	"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p." Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G. Cell 111:163-172(2002) [PubMed: 12408861] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
[4]	"Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore." Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P. Genes Dev. 16:101-113(2002) [PubMed: 11782448] [Abstract] Cited for: INTERACTION WITH CTF3; CTF19 AND MCM22, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49219 Genomic DNA. Translation: CAA89166.1. Z71255 Genomic DNA. Translation: CAA94993.1.
PIR	S54070.
RefSeq	NP_015371.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1430N.
IntAct	Q12262. 25 interactions.
STRING	Q12262.
Genome annotation databases
Ensembl	YPR046W; YPR046W; YPR046W; Saccharomyces cerevisiae. [Genome view]
GeneID	856159.
KEGG	sce:YPR046W.
NMPDR	fig|4932.3.peg.6507.
Organism-specific databases
CYGD	YPR046w.
SGD	S000006250. MCM16.
Phylogenomic databases
HOGENOM	Q12262.
OMA	CALEREH
OrthoDB	EOG97WQ5C
Gene expression databases
ArrayExpress	Q12262.
Genevestigator	Q12262.
GermOnline	YPR046W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	981298.

Entry information

Entry name

MCM16_YEAST

Accession

Primary (citable) accession number: Q12262

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 1, 1996
Last modified:	November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)