Q12262 (MCM16_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Central kinetochore subunit MCM16

Alternative name(s):
Minichromosome maintenance protein 16

Gene names

Name:	MCM16
Ordered Locus Names:	YPR046W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Ref.3
Subunit structure	Heterotrimer. CTF3, MCM16 and MCM22 form the CTF3 kinetochore subcomplex. The CTF3 subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CTF19. Ref.5
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Associated with kinetochores. Ref.5 Ref.6
Miscellaneous	Present with 279 molecules/cell in log phase SD medium. Ref.7

Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Domain	Coiled coil
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW establishment of mitotic sister chromatid cohesion Inferred from mutant phenotype. Source: SGD meiosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	condensed nuclear chromosome kinetochore Inferred from direct assay Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CTF19	Q02732	2	EBI-31487,EBI-5199
CTF3	Q12748	3	EBI-31487,EBI-30457
MCM22	P47167	7	EBI-31487,EBI-25691

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Central kinetochore subunit MCM16		PRO_0000096281
Regions
	Coiled coil	112 – 171	60	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q12262 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 73E00DC745D54FF0	FASTA	181	21,138
	10 20 30 40 50 60 MTNSSEKQWE RIQQLEKEHV EVYRELLITL DRLYLIRKHN HAVILSHTQQ RLLEIRHQLQ 70 80 90 100 110 120 INLEKTALLI RLLEKPDNTN VLFTKLQNLL EESNSLDYEL LQSLGAQSSL HKQLIESRAE 130 140 150 160 170 180 RDELMSKLIE LSSKFPKPTI PPDDSDTAGK QVEVEKENET IQELMIALQI HSGYTNISYT I

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The MCM16 gene of the yeast Saccharomyces cerevisiae is required for chromosome segregation." Sanyal K., Ghosh S.K., Sinha P. Mol. Gen. Genet. 260:242-250(1998) [PubMed: 9862478] [Abstract] Cited for: FUNCTION.
[4]	"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p." Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G. Cell 111:163-172(2002) [PubMed: 12408861] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
[5]	"Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore." Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P. Genes Dev. 16:101-113(2002) [PubMed: 11782448] [Abstract] Cited for: INTERACTION WITH CTF3; CTF19 AND MCM22, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49219 Genomic DNA. Translation: CAA89166.1. Z71255 Genomic DNA. Translation: CAA94993.1. BK006949 Genomic DNA. Translation: DAA11470.1.
PIR	S54070.
RefSeq	NP_015371.1. NM_001184143.1.
3D structure databases
ProteinModelPortal	Q12262.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1430N.
IntAct	Q12262. 20 interactions.
MINT	MINT-393701.
STRING	Q12262.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPR046W; YPR046W; YPR046W.
GeneID	856159.
KEGG	sce:YPR046W.
NMPDR	fig|4932.3.peg.6507.
Organism-specific databases
CYGD	YPR046w.
SGD	S000006250. MCM16.
Phylogenomic databases
eggNOG	fuNOG12885.
OMA	CALEREH.
OrthoDB	EOG4K9FP3.
Gene expression databases
ArrayExpress	Q12262.
Genevestigator	Q12262.
GermOnline	YPR046W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	981298.

Entry information

Entry name

MCM16_YEAST

Accession

Primary (citable) accession number: Q12262
Secondary accession number(s): D6W454

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program