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Reviewed, UniProtKB/Swiss-Prot Q12262 (MCM16_YEAST)

Last modified November 24, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Central kinetochore subunit MCM16
Alternative name(s):
    Minichromosome maintenance protein 16
Gene names
Name: MCM16
Ordered Locus Names: YPR046W
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length181 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation. Ref.2

Subunit structure

Heterotrimer. CTF3, MCM16 and MCM22 form the CTF3 kinetochore subcomplex. The CTF3 subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CTF19. Ref.4

Subcellular location

Nucleus. Kinetochore. Note: Associated with kinetochores. Ref.4 Ref.5

Miscellaneous

Present with 279 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
   Biological processCell cycle
Cell division
Meiosis
Mitosis
   Cellular componentKinetochore
Nucleus
   DomainCoiled coil
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcell division

Inferred from electronic annotation. Source: UniProtKB-KW

chromosome segregation Ref.4

Inferred from physical interaction. Source: SGD

meiosis

Inferred from electronic annotation. Source: UniProtKB-KW

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcondensed nuclear chromosome kinetochore Ref.4

Inferred from direct assay. Source: SGD

   Molecular functionprotein binding Ref.4

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 181181Central kinetochore subunit MCM16
PRO_0000096281

Regions

Coiled coil112 – 17160 Potential

Sequences

Sequence LengthMass (Da)Tools
Q12262-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 73E00DC745D54FF0

FASTA18121,138
        10         20         30         40         50         60 
MTNSSEKQWE RIQQLEKEHV EVYRELLITL DRLYLIRKHN HAVILSHTQQ RLLEIRHQLQ 

        70         80         90        100        110        120 
INLEKTALLI RLLEKPDNTN VLFTKLQNLL EESNSLDYEL LQSLGAQSSL HKQLIESRAE 

       130        140        150        160        170        180 
RDELMSKLIE LSSKFPKPTI PPDDSDTAGK QVEVEKENET IQELMIALQI HSGYTNISYT 


I 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The MCM16 gene of the yeast Saccharomyces cerevisiae is required for chromosome segregation."
Sanyal K., Ghosh S.K., Sinha P.
Mol. Gen. Genet. 260:242-250(1998) [PubMed: 9862478] [Abstract]
Cited for: FUNCTION.
[3]"Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
Cell 111:163-172(2002) [PubMed: 12408861] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
[4]"Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore."
Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P.
Genes Dev. 16:101-113(2002) [PubMed: 11782448] [Abstract]
Cited for: INTERACTION WITH CTF3; CTF19 AND MCM22, SUBCELLULAR LOCATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed: 14562095] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

Z49219 Genomic DNA. Translation: CAA89166.1.
Z71255 Genomic DNA. Translation: CAA94993.1.
PIRS54070.
RefSeqNP_015371.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1430N.
IntActQ12262. 25 interactions.
STRINGQ12262.

Genome annotation databases

EnsemblYPR046W; YPR046W; YPR046W; Saccharomyces cerevisiae. [Genome view]
GeneID856159.
KEGGsce:YPR046W.
NMPDRfig|4932.3.peg.6507.

Organism-specific databases

CYGDYPR046w.
SGDS000006250. MCM16.

Phylogenomic databases

HOGENOMQ12262.
OMACALEREH
OrthoDBEOG97WQ5C

Gene expression databases

ArrayExpressQ12262.
GenevestigatorQ12262.
GermOnlineYPR046W. Saccharomyces cerevisiae.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio981298.

Entry information

Entry nameMCM16_YEAST
AccessionPrimary (citable) accession number: Q12262
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 1, 1996
Last modified: November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents