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Protein

Polyamine transporter 4

Gene

TPO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermidine, spermine and the antimalarial drug quinidine, but not quinine, chloroquine and mefloquine.3 Publications

Miscellaneous

Present with 21400 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • spermidine transport Source: SGD
  • spermine transport Source: SGD

Keywordsi

Biological processAntiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33763-MONOMER

Protein family/group databases

TCDBi2.A.1.2.64 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 4
Gene namesi
Name:TPO4
Ordered Locus Names:YOR273C
ORF Names:O5440
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR273C
SGDiS000005799 TPO4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99CytoplasmicSequence analysisAdd BLAST99
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 128ExtracellularSequence analysis8
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 157CytoplasmicSequence analysis8
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 187ExtracellularSequence analysis9
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 215CytoplasmicSequence analysis7
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 246ExtracellularSequence analysis10
Transmembranei247 – 267HelicalSequence analysisAdd BLAST21
Topological domaini268 – 316CytoplasmicSequence analysisAdd BLAST49
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Topological domaini338 – 355ExtracellularSequence analysisAdd BLAST18
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 423CytoplasmicSequence analysisAdd BLAST47
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Topological domaini445 – 456ExtracellularSequence analysisAdd BLAST12
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Topological domaini478 – 486CytoplasmicSequence analysis9
Transmembranei487 – 509HelicalSequence analysisAdd BLAST23
Topological domaini510 – 518ExtracellularSequence analysis9
Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
Topological domaini540 – 659CytoplasmicSequence analysisAdd BLAST120

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627321 – 659Polyamine transporter 4Add BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei589PhosphothreonineCombined sources1
Modified residuei606PhosphothreonineCombined sources1
Modified residuei608PhosphothreonineCombined sources1
Modified residuei633PhosphoserineCombined sources1
Modified residuei646PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12256
PaxDbiQ12256
PRIDEiQ12256

PTM databases

iPTMnetiQ12256

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34662, 70 interactors
DIPiDIP-4096N
IntActiQ12256, 22 interactors
MINTiQ12256
STRINGi4932.YOR273C

Structurei

3D structure databases

ProteinModelPortaliQ12256
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227
HOGENOMiHOG000160689
InParanoidiQ12256
OMAiFPPIYVA
OrthoDBiEOG092C2079

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR005829 Sugar_transporter_CS
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
PROSITEiView protein in PROSITE
PS00216 SUGAR_TRANSPORT_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q12256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK
60 70 80 90 100
RSEPNADSNN MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF
110 120 130 140 150
LCLVVTMGSS LYVSSVPELV ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS
160 170 180 190 200
EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM RIILPLRFLS GVFASPALSV
210 220 230 240 250
GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT EAKGWRWSEW
260 270 280 290 300
IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT
310 320 330 340 350
TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM
360 370 380 390 400
SMGISGLPFI GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK
410 420 430 440 450
RTTPYRGARD AETGELLPVV PEKFLIACKF GSVALPIGLF WQAWTARSDV
460 470 480 490 500
HWMAPVAAGV PFGFGLILIF FSVLMYFSTC YPPLTVASCL AANNLLRYVM
510 520 530 540 550
SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK WGSKLRHKSQ
560 570 580 590 600
FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER
610 620 630 640 650
LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM

ATDASARMV
Length:659
Mass (Da):73,223
Last modified:November 1, 1996 - v1
Checksum:i7F2BD0EEC5E408FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89633 Genomic DNA Translation: CAA61779.1
Z75181 Genomic DNA Translation: CAA99498.1
BK006948 Genomic DNA Translation: DAA11039.1
PIRiS67175
RefSeqiNP_014916.1, NM_001183692.1

Genome annotation databases

EnsemblFungiiYOR273C; YOR273C; YOR273C
GeneIDi854447
KEGGisce:YOR273C

Similar proteinsi

Entry informationi

Entry nameiTPO4_YEAST
AccessioniPrimary (citable) accession number: Q12256
Secondary accession number(s): D6W2X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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