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Protein

Polyamine transporter 4

Gene

TPO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermidine, spermine and the antimalarial drug quinidine, but not quinine, chloroquine and mefloquine.3 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • spermidine transport Source: SGD
  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33763-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.64. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 4
Gene namesi
Name:TPO4
Ordered Locus Names:YOR273C
ORF Names:O5440
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR273C.
SGDiS000005799. TPO4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9999CytoplasmicSequence analysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence analysisAdd
BLAST
Topological domaini121 – 1288ExtracellularSequence analysis
Transmembranei129 – 14921HelicalSequence analysisAdd
BLAST
Topological domaini150 – 1578CytoplasmicSequence analysis
Transmembranei158 – 17821HelicalSequence analysisAdd
BLAST
Topological domaini179 – 1879ExtracellularSequence analysis
Transmembranei188 – 20821HelicalSequence analysisAdd
BLAST
Topological domaini209 – 2157CytoplasmicSequence analysis
Transmembranei216 – 23621HelicalSequence analysisAdd
BLAST
Topological domaini237 – 24610ExtracellularSequence analysis
Transmembranei247 – 26721HelicalSequence analysisAdd
BLAST
Topological domaini268 – 31649CytoplasmicSequence analysisAdd
BLAST
Transmembranei317 – 33721HelicalSequence analysisAdd
BLAST
Topological domaini338 – 35518ExtracellularSequence analysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST
Topological domaini377 – 42347CytoplasmicSequence analysisAdd
BLAST
Transmembranei424 – 44421HelicalSequence analysisAdd
BLAST
Topological domaini445 – 45612ExtracellularSequence analysisAdd
BLAST
Transmembranei457 – 47721HelicalSequence analysisAdd
BLAST
Topological domaini478 – 4869CytoplasmicSequence analysis
Transmembranei487 – 50923HelicalSequence analysisAdd
BLAST
Topological domaini510 – 5189ExtracellularSequence analysis
Transmembranei519 – 53921HelicalSequence analysisAdd
BLAST
Topological domaini540 – 659120CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 659659Polyamine transporter 4PRO_0000262732Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei589 – 5891PhosphothreonineCombined sources
Modified residuei606 – 6061PhosphothreonineCombined sources
Modified residuei608 – 6081PhosphothreonineCombined sources
Modified residuei633 – 6331PhosphoserineCombined sources
Modified residuei646 – 6461PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12256.

PTM databases

iPTMnetiQ12256.

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34662. 46 interactions.
DIPiDIP-4096N.
IntActiQ12256. 22 interactions.
MINTiMINT-549572.

Structurei

3D structure databases

ProteinModelPortaliQ12256.
SMRiQ12256. Positions 107-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ12256.
OMAiCCAPETY.
OrthoDBiEOG77M8XK.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK
60 70 80 90 100
RSEPNADSNN MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF
110 120 130 140 150
LCLVVTMGSS LYVSSVPELV ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS
160 170 180 190 200
EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM RIILPLRFLS GVFASPALSV
210 220 230 240 250
GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT EAKGWRWSEW
260 270 280 290 300
IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT
310 320 330 340 350
TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM
360 370 380 390 400
SMGISGLPFI GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK
410 420 430 440 450
RTTPYRGARD AETGELLPVV PEKFLIACKF GSVALPIGLF WQAWTARSDV
460 470 480 490 500
HWMAPVAAGV PFGFGLILIF FSVLMYFSTC YPPLTVASCL AANNLLRYVM
510 520 530 540 550
SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK WGSKLRHKSQ
560 570 580 590 600
FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER
610 620 630 640 650
LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM

ATDASARMV
Length:659
Mass (Da):73,223
Last modified:November 1, 1996 - v1
Checksum:i7F2BD0EEC5E408FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89633 Genomic DNA. Translation: CAA61779.1.
Z75181 Genomic DNA. Translation: CAA99498.1.
BK006948 Genomic DNA. Translation: DAA11039.1.
PIRiS67175.
RefSeqiNP_014916.1. NM_001183692.1.

Genome annotation databases

EnsemblFungiiYOR273C; YOR273C; YOR273C.
GeneIDi854447.
KEGGisce:YOR273C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89633 Genomic DNA. Translation: CAA61779.1.
Z75181 Genomic DNA. Translation: CAA99498.1.
BK006948 Genomic DNA. Translation: DAA11039.1.
PIRiS67175.
RefSeqiNP_014916.1. NM_001183692.1.

3D structure databases

ProteinModelPortaliQ12256.
SMRiQ12256. Positions 107-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34662. 46 interactions.
DIPiDIP-4096N.
IntActiQ12256. 22 interactions.
MINTiMINT-549572.

Protein family/group databases

TCDBi2.A.1.2.64. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ12256.

Proteomic databases

MaxQBiQ12256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR273C; YOR273C; YOR273C.
GeneIDi854447.
KEGGisce:YOR273C.

Organism-specific databases

EuPathDBiFungiDB:YOR273C.
SGDiS000005799. TPO4.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ12256.
OMAiCCAPETY.
OrthoDBiEOG77M8XK.

Enzyme and pathway databases

BioCyciYEAST:G3O-33763-MONOMER.

Miscellaneous databases

PROiQ12256.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence analysis of the VPH1-SNF2 region on chromosome XV of Saccharomyces cerevisiae."
    Cheret G., Bernardi A., Sor F.J.
    Yeast 12:1059-1064(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification of Saccharomyces cerevisiae genes conferring resistance to quinoline ring-containing antimalarial drugs."
    Delling U., Raymond M., Schurr E.
    Antimicrob. Agents Chemother. 42:1034-1041(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Multiple polyamine transport systems on the vacuolar membrane in yeast."
    Tomitori H., Kashiwagi K., Asakawa T., Kakinuma Y., Michael A.J., Igarashi K.
    Biochem. J. 353:681-688(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Localization and function of the yeast multidrug transporter Tpo1p."
    Albertsen M., Bellahn I., Kraemer R., Waffenschmidt S.
    J. Biol. Chem. 278:12820-12825(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively."
    Aranda A., del Olmo M.
    Appl. Environ. Microbiol. 70:1913-1922(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-606 AND THR-608, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-589, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-633 AND SER-646, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTPO4_YEAST
AccessioniPrimary (citable) accession number: Q12256
Secondary accession number(s): D6W2X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 21400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.