Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyamine transporter 4

Gene

TPO4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell membrane polyamine/proton antiporter, involved in the detoxification of excess polyamines in the cytoplasm. Recognizes spermidine, spermine and the antimalarial drug quinidine, but not quinine, chloroquine and mefloquine.3 Publications

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • spermidine transmembrane transporter activity Source: SGD
  • spermine transmembrane transporter activity Source: SGD

GO - Biological processi

  • spermidine transport Source: SGD
  • spermine transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33763-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.64. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine transporter 4
Gene namesi
Name:TPO4
Ordered Locus Names:YOR273C
ORF Names:O5440
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR273C.
SGDiS000005799. TPO4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99CytoplasmicSequence analysisAdd BLAST99
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121 – 128ExtracellularSequence analysis8
Transmembranei129 – 149HelicalSequence analysisAdd BLAST21
Topological domaini150 – 157CytoplasmicSequence analysis8
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 187ExtracellularSequence analysis9
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 215CytoplasmicSequence analysis7
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 246ExtracellularSequence analysis10
Transmembranei247 – 267HelicalSequence analysisAdd BLAST21
Topological domaini268 – 316CytoplasmicSequence analysisAdd BLAST49
Transmembranei317 – 337HelicalSequence analysisAdd BLAST21
Topological domaini338 – 355ExtracellularSequence analysisAdd BLAST18
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 423CytoplasmicSequence analysisAdd BLAST47
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Topological domaini445 – 456ExtracellularSequence analysisAdd BLAST12
Transmembranei457 – 477HelicalSequence analysisAdd BLAST21
Topological domaini478 – 486CytoplasmicSequence analysis9
Transmembranei487 – 509HelicalSequence analysisAdd BLAST23
Topological domaini510 – 518ExtracellularSequence analysis9
Transmembranei519 – 539HelicalSequence analysisAdd BLAST21
Topological domaini540 – 659CytoplasmicSequence analysisAdd BLAST120

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002627321 – 659Polyamine transporter 4Add BLAST659

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei589PhosphothreonineCombined sources1
Modified residuei606PhosphothreonineCombined sources1
Modified residuei608PhosphothreonineCombined sources1
Modified residuei633PhosphoserineCombined sources1
Modified residuei646PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ12256.
PRIDEiQ12256.

PTM databases

iPTMnetiQ12256.

Expressioni

Inductioni

By transcription factor HAA1 in response to acetaldehyde accumulation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi34662. 46 interactors.
DIPiDIP-4096N.
IntActiQ12256. 22 interactors.
MINTiMINT-549572.

Structurei

3D structure databases

ProteinModelPortaliQ12256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ12256.
OMAiCCAPETY.
OrthoDBiEOG092C2079.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q12256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSSLTKTES NSDPRTNIQQ VPKALDKNVT NSGNLDSTSS STGSITEDEK
60 70 80 90 100
RSEPNADSNN MTGGEPIDPR DLDWDGPDDP DNPHNWSSLK KWYTTMTSAF
110 120 130 140 150
LCLVVTMGSS LYVSSVPELV ERYHVSQTLA LAGLTFYLLG LSTVIGAPLS
160 170 180 190 200
EVFGRKPVYL FSLPVSMLFT MGVGLSNGHM RIILPLRFLS GVFASPALSV
210 220 230 240 250
GSGTILDIFD VDQVSVAMTY FVLSPFLGPV LSPIMAGFAT EAKGWRWSEW
260 270 280 290 300
IQLIAGGLIL PFIALMPETH KGIILRKRAK KRNIALKKFS REAQKEFLKT
310 320 330 340 350
TVTITILRPL KMLVVEPIVF VFSVYVAFIF AILFGFFEAY AVIYRGVYHM
360 370 380 390 400
SMGISGLPFI GIGVGLWIGA FFYLYIDRKY LFPKPPAGTQ PLTEKERTSK
410 420 430 440 450
RTTPYRGARD AETGELLPVV PEKFLIACKF GSVALPIGLF WQAWTARSDV
460 470 480 490 500
HWMAPVAAGV PFGFGLILIF FSVLMYFSTC YPPLTVASCL AANNLLRYVM
510 520 530 540 550
SSVFPLFTIQ MYTKMKIKWA STLFALVCVV MIPIPWVFEK WGSKLRHKSQ
560 570 580 590 600
FGYAAMEKEA ETEGGIDDVN AVDGELNLTR MTTLRTMETD PSTREKPGER
610 620 630 640 650
LSLRRTHTQP VPASFDREDG QHAQNRNEPI SNSLYSAIKD NEDGYSYTEM

ATDASARMV
Length:659
Mass (Da):73,223
Last modified:November 1, 1996 - v1
Checksum:i7F2BD0EEC5E408FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89633 Genomic DNA. Translation: CAA61779.1.
Z75181 Genomic DNA. Translation: CAA99498.1.
BK006948 Genomic DNA. Translation: DAA11039.1.
PIRiS67175.
RefSeqiNP_014916.1. NM_001183692.1.

Genome annotation databases

EnsemblFungiiYOR273C; YOR273C; YOR273C.
GeneIDi854447.
KEGGisce:YOR273C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89633 Genomic DNA. Translation: CAA61779.1.
Z75181 Genomic DNA. Translation: CAA99498.1.
BK006948 Genomic DNA. Translation: DAA11039.1.
PIRiS67175.
RefSeqiNP_014916.1. NM_001183692.1.

3D structure databases

ProteinModelPortaliQ12256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34662. 46 interactors.
DIPiDIP-4096N.
IntActiQ12256. 22 interactors.
MINTiMINT-549572.

Protein family/group databases

TCDBi2.A.1.2.64. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiQ12256.

Proteomic databases

MaxQBiQ12256.
PRIDEiQ12256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR273C; YOR273C; YOR273C.
GeneIDi854447.
KEGGisce:YOR273C.

Organism-specific databases

EuPathDBiFungiDB:YOR273C.
SGDiS000005799. TPO4.

Phylogenomic databases

GeneTreeiENSGT00620000088227.
HOGENOMiHOG000160689.
InParanoidiQ12256.
OMAiCCAPETY.
OrthoDBiEOG092C2079.

Enzyme and pathway databases

BioCyciYEAST:G3O-33763-MONOMER.

Miscellaneous databases

PROiQ12256.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPO4_YEAST
AccessioniPrimary (citable) accession number: Q12256
Secondary accession number(s): D6W2X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 21400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.