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Protein

Vacuolar v-SNARE NYV1

Gene

NYV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Vacuolar v-SNARE required for docking. Only involved in homotypic vacuole fusion. Required for Ca2+ efflux from the vacuolar lumen, a required signal for subsequent membrane fusion events, by inhibiting vacuolar Ca2+-ATPase PMC1 and promoting Ca2+ release when forming trans-SNARE assemblies during the docking step.4 Publications

Miscellaneous

Present with 1885 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

GO - Biological processi

  • exocytosis Source: GO_Central
  • vacuole fusion, non-autophagic Source: SGD
  • vesicle fusion Source: SGD
  • vesicle-mediated transport Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-32243-MONOMER

Protein family/group databases

TCDBi1.F.1.1.2 the synaptosomal vesicle fusion pore (svf-pore) family

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar v-SNARE NYV1
Short name:
R-SNARE NYV1
Alternative name(s):
New v-SNARE 1
Synaptobrevin NYV1
Gene namesi
Name:NYV1
Synonyms:MAM2
Ordered Locus Names:YLR093C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR093C
SGDiS000004083 NYV1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 231CytoplasmicSequence analysisAdd BLAST231
Transmembranei232 – 252Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini253VacuolarSequence analysis1

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067801 – 253Vacuolar v-SNARE NYV1Add BLAST253

Proteomic databases

MaxQBiQ12255
PaxDbiQ12255
PRIDEiQ12255
TopDownProteomicsiQ12255

PTM databases

iPTMnetiQ12255

Interactioni

Subunit structurei

Present in a pentameric cis-SNARE complex composed of the v-SNAREs NYV1, VTI1 and YKT6, and the t-SNAREs VAM3 and VAM7 on vacuolar membranes. Interacts in trans with the cognate t-SNARE VAM3 during the docking step of homotypic vacuolar fusion. Interacts with the vacuolar transporter chaperone (VTC) complex and the vacuolar Ca2+-ATPase PMC1.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • SNAP receptor activity Source: SGD
  • SNARE binding Source: GO_Central

Protein-protein interaction databases

BioGridi31366, 80 interactors
DIPiDIP-2248N
IntActiQ12255, 16 interactors
MINTiQ12255
STRINGi4932.YLR093C

Structurei

Secondary structure

1253
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 16Combined sources10
Beta strandi20 – 22Combined sources3
Beta strandi26 – 30Combined sources5
Helixi43 – 52Combined sources10
Helixi55 – 57Combined sources3
Beta strandi65 – 68Combined sources4
Beta strandi74 – 81Combined sources8
Beta strandi90 – 97Combined sources8
Helixi104 – 112Combined sources9
Helixi124 – 141Combined sources18
Turni142 – 144Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FZ0NMR-A1-149[»]
ProteinModelPortaliQ12255
SMRiQ12255
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ12255

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini167 – 227v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000113898
InParanoidiQ12255
KOiK08564
OMAiLIDGFDC
OrthoDBiEOG092C4MCV

Family and domain databases

Gene3Di3.30.450.230, 1 hit
InterProiView protein in InterPro
IPR038426 Nyv1_N
IPR001388 Synaptobrevin
IPR019005 Vacuolar_R-SNAR_Nyv1_N_dom
PfamiView protein in Pfam
PF09426 Nyv1_N, 1 hit
PF00957 Synaptobrevin, 1 hit
PRINTSiPR00219 SYNAPTOBREVN
PROSITEiView protein in PROSITE
PS50892 V_SNARE, 1 hit

Sequencei

Sequence statusi: Complete.

Q12255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRFNVSYVE VIKNGETISS CFQPFQKNEN YGTITSANEQ ITPVIFHNLI
60 70 80 90 100
MDMVLPKVVP IKGNKVTKMS MNLIDGFDCF YSTDDHDPKT VYVCFTLVDM
110 120 130 140 150
PKILPIRILS GLQEYESNAT NELLSSHVGQ ILDSFHEELV EYRNQTLNSS
160 170 180 190 200
GNGQSSNGNG QNTISDIGDA TEDQIKDVIQ IMNDNIDKFL ERQERVSLLV
210 220 230 240 250
DKTSQLNSSS NKFRRKAVNI KEIMWWQKVK NITLLTFTII LFVSAAFMFF

YLW
Length:253
Mass (Da):28,964
Last modified:October 17, 2006 - v2
Checksum:i494B587CF0F85997
GO

Sequence cautioni

The sequence AAB67537 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAT92805 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA97654 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73265 Genomic DNA Translation: CAA97654.1 Sequence problems.
U53876 Genomic DNA Translation: AAB67537.1 Sequence problems.
AY692786 Genomic DNA Translation: AAT92805.1 Sequence problems.
BK006945 Genomic DNA Translation: DAA09409.1
PIRiS64927
RefSeqiNP_013194.2, NM_001181980.1

Genome annotation databases

EnsemblFungiiYLR093C; YLR093C; YLR093C
GeneIDi850782
KEGGisce:YLR093C

Similar proteinsi

Entry informationi

Entry nameiNYV1_YEAST
AccessioniPrimary (citable) accession number: Q12255
Secondary accession number(s): D6VY93
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 17, 2006
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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