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Protein

26S proteasome regulatory subunit RPN5

Gene

RPN5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.1 Publication

Miscellaneous

Present with 5710 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein deneddylation Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-29544-MONOMER
ReactomeiR-SCE-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-SCE-174113 SCF-beta-TrCP mediated degradation of Emi1
R-SCE-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-SCE-382556 ABC-family proteins mediated transport
R-SCE-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-SCE-5668541 TNFR2 non-canonical NF-kB pathway
R-SCE-5687128 MAPK6/MAPK4 signaling
R-SCE-5689880 Ub-specific processing proteases
R-SCE-6798695 Neutrophil degranulation
R-SCE-68949 Orc1 removal from chromatin
R-SCE-69017 CDK-mediated phosphorylation and removal of Cdc6
R-SCE-69229 Ubiquitin-dependent degradation of Cyclin D1
R-SCE-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SCE-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-SCE-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit RPN5
Alternative name(s):
Proteasome non-ATPase subunit 5
Gene namesi
Name:RPN5
Synonyms:NAS5
Ordered Locus Names:YDL147W
ORF Names:D1572
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL147W
SGDiS000002306 RPN5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001738652 – 44526S proteasome regulatory subunit RPN5Add BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1

Post-translational modificationi

N-acetylated by NAT1.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ12250
PaxDbiQ12250
PRIDEiQ12250

PTM databases

iPTMnetiQ12250

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31914, 611 interactors
DIPiDIP-1578N
IntActiQ12250, 87 interactors
MINTiQ12250
STRINGi4932.YDL147W

Structurei

Secondary structure

1445
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi32 – 35Combined sources4
Helixi37 – 41Combined sources5
Helixi42 – 44Combined sources3
Helixi49 – 68Combined sources20
Turni69 – 71Combined sources3
Helixi72 – 84Combined sources13
Helixi94 – 106Combined sources13
Helixi111 – 125Combined sources15
Helixi135 – 147Combined sources13
Helixi153 – 160Combined sources8
Turni161 – 164Combined sources4
Helixi165 – 167Combined sources3
Turni168 – 170Combined sources3
Helixi173 – 190Combined sources18
Helixi194 – 201Combined sources8
Helixi205 – 209Combined sources5
Helixi211 – 231Combined sources21
Helixi235 – 245Combined sources11
Helixi248 – 252Combined sources5
Helixi254 – 270Combined sources17
Helixi275 – 286Combined sources12
Helixi288 – 291Combined sources4
Helixi295 – 304Combined sources10
Helixi311 – 324Combined sources14
Turni326 – 328Combined sources3
Beta strandi331 – 334Combined sources4
Helixi337 – 356Combined sources20
Beta strandi357 – 361Combined sources5
Helixi362 – 369Combined sources8
Helixi373 – 385Combined sources13
Beta strandi392 – 394Combined sources3
Turni395 – 398Combined sources4
Beta strandi399 – 401Combined sources3
Helixi408 – 439Combined sources32

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J47electron microscopy-P409-442[»]
3JCKelectron microscopy3.50B1-445[»]
3JCOelectron microscopy4.80P1-445[»]
3JCPelectron microscopy4.60P1-445[»]
4CR2electron microscopy7.70P1-445[»]
4CR3electron microscopy9.30P1-445[»]
4CR4electron microscopy8.80P1-445[»]
5A5Belectron microscopy9.50P1-445[»]
5MPBelectron microscopy7.80P1-445[»]
5MPCelectron microscopy7.70P1-445[»]
5MPDelectron microscopy4.10P1-445[»]
5MPEelectron microscopy4.50P1-445[»]
5WVIelectron microscopy6.30P1-445[»]
5WVKelectron microscopy4.20P1-445[»]
ProteinModelPortaliQ12250
SMRiQ12250
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini233 – 407PCIPROSITE-ProRule annotationAdd BLAST175

Sequence similaritiesi

Belongs to the proteasome subunit p55 family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000111110
HOGENOMiHOG000194265
InParanoidiQ12250
KOiK03035
OMAiKQAITKM
OrthoDBiEOG092C2UIQ

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000717 PCI_dom
IPR035297 PSMD12
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10855:SF1 PTHR10855:SF1, 1 hit
PfamiView protein in Pfam
PF01399 PCI, 1 hit
SMARTiView protein in SMART
SM00088 PINT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q12250-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRDAPIKAD KDYSQILKEE FPKIDSLAQN DCNSALDQLL VLEKKTRQAS
60 70 80 90 100
DLASSKEVLA KIVDLLASRN KWDDLNEQLT LLSKKHGQLK LSIQYMIQKV
110 120 130 140 150
MEYLKSSKSL DLNTRISVIE TIRVVTENKI FVEVERARVT KDLVEIKKEE
160 170 180 190 200
GKIDEAADIL CELQVETYGS MEMSEKIQFI LEQMELSILK GDYSQATVLS
210 220 230 240 250
RKILKKTFKN PKYESLKLEY YNLLVKISLH KREYLEVAQY LQEIYQTDAI
260 270 280 290 300
KSDEAKWKPV LSHIVYFLVL SPYGNLQNDL IHKIQNDNNL KKLESQESLV
310 320 330 340 350
KLFTTNELMR WPIVQKTYEP VLNEDDLAFG GEANKHHWED LQKRVIEHNL
360 370 380 390 400
RVISEYYSRI TLLRLNELLD LTESQTETYI SDLVNQGIIY AKVNRPAKIV
410 420 430 440
NFEKPKNSSQ LLNEWSHNVD ELLEHIETIG HLITKEEIMH GLQAK
Length:445
Mass (Da):51,768
Last modified:January 23, 2007 - v3
Checksum:iE5DC7F8E6AE27736
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X97751 Genomic DNA Translation: CAA66344.1
Z74195 Genomic DNA Translation: CAA98721.1
BK006938 Genomic DNA Translation: DAA11711.1
PIRiS67695
RefSeqiNP_010134.1, NM_001180207.1

Genome annotation databases

EnsemblFungiiYDL147W; YDL147W; YDL147W
GeneIDi851408
KEGGisce:YDL147W

Similar proteinsi

Entry informationi

Entry nameiRPN5_YEAST
AccessioniPrimary (citable) accession number: Q12250
Secondary accession number(s): D6VRK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health