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Q12250 (RPN5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
26S proteasome regulatory subunit RPN5
Alternative name(s):
Proteasome non-ATPase subunit 5
Gene names
Name:RPN5
Synonyms:NAS5
Ordered Locus Names:YDL147W
ORF Names:D1572
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length445 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Ref.5

Post-translational modification

N-acetylated by NAT1.

Miscellaneous

Present with 5710 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the proteasome subunit p55 family.

Contains 1 PCI domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 44544426S proteasome regulatory subunit RPN5
PRO_0000173865

Regions

Domain231 – 404174PCI

Amino acid modifications

Modified residue21N-acetylserine Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q12250 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: E5DC7F8E6AE27736

FASTA44551,768
        10         20         30         40         50         60 
MSRDAPIKAD KDYSQILKEE FPKIDSLAQN DCNSALDQLL VLEKKTRQAS DLASSKEVLA 

        70         80         90        100        110        120 
KIVDLLASRN KWDDLNEQLT LLSKKHGQLK LSIQYMIQKV MEYLKSSKSL DLNTRISVIE 

       130        140        150        160        170        180 
TIRVVTENKI FVEVERARVT KDLVEIKKEE GKIDEAADIL CELQVETYGS MEMSEKIQFI 

       190        200        210        220        230        240 
LEQMELSILK GDYSQATVLS RKILKKTFKN PKYESLKLEY YNLLVKISLH KREYLEVAQY 

       250        260        270        280        290        300 
LQEIYQTDAI KSDEAKWKPV LSHIVYFLVL SPYGNLQNDL IHKIQNDNNL KKLESQESLV 

       310        320        330        340        350        360 
KLFTTNELMR WPIVQKTYEP VLNEDDLAFG GEANKHHWED LQKRVIEHNL RVISEYYSRI 

       370        380        390        400        410        420 
TLLRLNELLD LTESQTETYI SDLVNQGIIY AKVNRPAKIV NFEKPKNSSQ LLNEWSHNVD 

       430        440 
ELLEHIETIG HLITKEEIMH GLQAK

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 23 kb region on the left arm of yeast chromosome IV."
Delaveau T.T.D., Blugeon C., Jacq C., Perea J.
Yeast 12:1587-1592(1996) [PubMed: 8972581] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed: 9169867] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"N-terminal modifications of the 19S regulatory particle subunits of the yeast proteasome."
Kimura Y., Saeki Y., Yokosawa H., Polevoda B., Sherman F., Hirano H.
Arch. Biochem. Biophys. 409:341-348(2003) [PubMed: 12504901] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-8, ACETYLATION AT SER-2.
[5]"cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome."
Saito A., Watanabe T.K., Shimada Y., Fujiwara T., Slaughter C.A., DeMartino G.N., Tanahashi N., Tanaka K.
Gene 203:241-250(1997) [PubMed: 9426256] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X97751 Genomic DNA. Translation: CAA66344.1.
Z74195 Genomic DNA. Translation: CAA98721.1.
BK006938 Genomic DNA. Translation: DAA11711.1.
PIRS67695.
RefSeqNP_010134.1. NM_001180207.1.

3D structure databases

ProteinModelPortalQ12250.
SMRQ12250. Positions 335-410.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-1578N.
IntActQ12250. 56 interactions.
MINTMINT-391026.
STRINGQ12250.

Proteomic databases

PeptideAtlasQ12250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDL147W; YDL147W; YDL147W.
GeneID851408.
KEGGsce:YDL147W.
NMPDRfig|4932.3.peg.871.

Organism-specific databases

SGDS000002306. RPN5.

Phylogenomic databases

eggNOGfuNOG04501.
GeneTreeEFGT00050000004538.
HOGENOMHBG749871.
OMAWDALNEN.
OrthoDBEOG41G6CN.

Gene expression databases

ArrayExpressQ12250.
GenevestigatorQ12250.
GermOnlineYDL147W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR000717. PCI_dom.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]
Gene3DG3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KOK03035.
PfamPF01399. PCI. 1 hit.
[Graphical view]
SMARTSM00088. PINT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968585.

Entry information

Entry nameRPN5_YEAST
AccessionPrimary (citable) accession number: Q12250
Secondary accession number(s): D6VRK1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents