Reviewed,
UniProtKB/Swiss-Prot Q12248 (DAD1_YEAST)
Last modified
January 19, 2010.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: DASH complex subunit DAD1 Alternative name(s): Outer kinetochore protein DAD1 DUO1 and DAM1-interacting protein 1 | ||||
| Gene names |
| ||||
| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||
| Taxonomic identifier | 4932 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 94 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. Ref.6 Ref.8 Ref.10 |
| Subunit structure | The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1. Ref.9 |
| Subcellular location | Nucleus. Cytoplasm › cytoskeleton › spindle. Kinetochore. Note: Associates with the mitotic spindle and the kinetochore. Ref.4 |
| Miscellaneous | Present with 799 molecules/cell in log phase SD medium. Ref.5 |
| Sequence similarities | Belongs to the DASH complex DAD1 family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| DAM1 | P53267 | 1 | EBI-35662,EBI-23268 | |
| DUO1 | P53168 | 2 | EBI-35662,EBI-23800 | |
| SPC34 | P36131 | 1 | EBI-35662,EBI-26401 |
Sequence annotation (Features)
Sequences
References
| « Hide 'large scale' references | |
| [1] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. Zaccaria P.Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [2] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [3] | "The mitotic spindle is required for loading of the DASH complex onto the kinetochore." Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J. Genes Dev. 16:183-197(2002) [PubMed: 11799062] [Abstract] Cited for: COMPONENT OF DASH COMPLEX. |
| [4] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [5] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [6] | "Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex." Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G. Mol. Cell 17:277-290(2005) [PubMed: 15664196] [Abstract] Cited for: FUNCTION. |
| [7] | "A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-87; SER-89 AND SER-91, MASS SPECTROMETRY. |
| [8] | "The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends." Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G. Nature 440:565-569(2006) [PubMed: 16415853] [Abstract] Cited for: FUNCTION. |
| [9] | "Molecular architecture of a kinetochore-microtubule attachment site." Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D. Nat. Cell Biol. 8:581-585(2006) [PubMed: 16715078] [Abstract] Cited for: SUBUNIT. |
| [10] | "The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement." Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N. Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed: 16777964] [Abstract] Cited for: FUNCTION. |
| [11] | "The yeast DASH complex forms closed rings on microtubules." Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C. Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed: 15640796] [Abstract] Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z74312 Genomic DNA. Translation: CAA98836.1. Z49770 Genomic DNA. Translation: CAA89841.1. AY557635 Genomic DNA. Translation: AAS55961.1. |
| PIR | S54639. |
| RefSeq | NP_010299.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-1296N. |
| IntAct | Q12248. 14 interactions. |
| STRING | Q12248. |
Genome annotation databases | |
| Ensembl | YDR016C; YDR016C; YDR016C; Saccharomyces cerevisiae. [Genome view] |
| GeneID | 851579. |
| KEGG | sce:YDR016C. |
| NMPDR | fig|4932.3.peg.1042. |
Organism-specific databases | |
| CYGD | YDR016c. |
| SGD | S000002423. DAD1. |
Phylogenomic databases | |
| eggNOG | fuNOG12986. |
| HOGENOM | HBG398797. |
| OMA | IGKEFDD. |
| OrthoDB | EOG9GJ15F. |
| PhylomeDB | Q12248. |
Gene expression databases | |
| ArrayExpress | Q12248. |
| Genevestigator | Q12248. |
| GermOnline | YDR016C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR013958. DASH_Dad1. [Graphical view] |
| Pfam | PF08649. DASH_Dad1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 969040. |
Entry information
| Entry name | DAD1_YEAST | ||||||||
| Accession | Primary (citable) accession number: Q12248 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |

Clusters with


