Q12248 (DAD1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DASH complex subunit DAD1

Alternative name(s):
DUO1 and DAM1-interacting protein 1
Outer kinetochore protein DAD1

Gene names

Name:	DAD1
Ordered Locus Names:	YDR016C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids. Ref.7 Ref.9 Ref.11
Subunit structure	The DASH complex is an approximately 210 kDa heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry of one copy of each subunit. DASH oligomerizes into a 50 nm ring composed of about 16 molecules that encircles the microtubule. Integrity of the complex and interactions with central kinetochore proteins are regulated by the spindle assembly checkpoint kinase IPL1. Ref.10
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › spindle. Chromosome › centromere › kinetochore. Note: Associates with the mitotic spindle and the kinetochore. Ref.5
Miscellaneous	Present with 799 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the DASH complex DAD1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Chromosome partition Mitosis
Cellular component	Centromere Chromosome Cytoplasm Cytoskeleton Kinetochore Microtubule Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic spindle organization in nucleus Inferred from physical interaction. Source: SGD regulation of microtubule polymerization or depolymerization Inferred from direct assay. Source: SGD
Cellular component	DASH complex Inferred from direct assay Ref.12Ref.7. Source: SGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW spindle Inferred from physical interaction. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct structural constituent of cytoskeleton Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAM1	P53267	4	EBI-35662,EBI-23268
DUO1	P53168	4	EBI-35662,EBI-23800

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 94	94	DASH complex subunit DAD1		PRO_0000127613
Amino acid modifications
	Modified residue	87	1	Phosphothreonine Ref.8
	Modified residue	89	1	Phosphoserine Ref.8
	Modified residue	91	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Q12248 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6D27737AC5A39D51	FASTA	94	10,516
	10 20 30 40 50 60 MMASTSNDEE KLISTTDKYF IEQRNIVLQE INETMNSILN GLNGLNISLE SSIAVGREFQ 70 80 90 SVSDLWKTLY DGLESLSDEA PIDEQPTLSQ SKTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The mitotic spindle is required for loading of the DASH complex onto the kinetochore." Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J. Genes Dev. 16:183-197(2002) [PubMed: 11799062] [Abstract] Cited for: COMPONENT OF DASH COMPLEX.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Formation of a dynamic kinetochore-microtubule interface through assembly of the Dam1 ring complex." Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., Drubin D.G., Nogales E., Barnes G. Mol. Cell 17:277-290(2005) [PubMed: 15664196] [Abstract] Cited for: FUNCTION.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-87; SER-89 AND SER-91, MASS SPECTROMETRY.
[9]	"The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends." Westermann S., Wang H.-W., Avila-Sakar A., Drubin D.G., Nogales E., Barnes G. Nature 440:565-569(2006) [PubMed: 16415853] [Abstract] Cited for: FUNCTION.
[10]	"Molecular architecture of a kinetochore-microtubule attachment site." Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D. Nat. Cell Biol. 8:581-585(2006) [PubMed: 16715078] [Abstract] Cited for: SUBUNIT.
[11]	"The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement." Asbury C.L., Gestaut D.R., Powers A.F., Franck A.D., Davis T.N. Proc. Natl. Acad. Sci. U.S.A. 103:9873-9878(2006) [PubMed: 16777964] [Abstract] Cited for: FUNCTION.
[12]	"The yeast DASH complex forms closed rings on microtubules." Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C. Nat. Struct. Mol. Biol. 12:138-143(2005) [PubMed: 15640796] [Abstract] Cited for: ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z74312 Genomic DNA. Translation: CAA98836.1. Z49770 Genomic DNA. Translation: CAA89841.1. AY557635 Genomic DNA. Translation: AAS55961.1. BK006938 Genomic DNA. Translation: DAA11862.1.
PIR	S54639.
RefSeq	NP_010299.1. NM_001180324.1.
3D structure databases
ProteinModelPortal	Q12248.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1296N.
IntAct	Q12248. 9 interactions.
MINT	MINT-409635.
STRING	Q12248.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR016C; YDR016C; YDR016C.
GeneID	851579.
KEGG	sce:YDR016C.
NMPDR	fig|4932.3.peg.1042.
Organism-specific databases
CYGD	YDR016c.
SGD	S000002423. DAD1.
Phylogenomic databases
eggNOG	fuNOG12986.
HOGENOM	HBG398797.
OMA	IGKEFDD.
OrthoDB	EOG4T4H58.
Gene expression databases
ArrayExpress	Q12248.
Genevestigator	Q12248.
GermOnline	YDR016C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013958. DASH_Dad1. [Graphical view]
KO	K11553.
Pfam	PF08649. DASH_Dad1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969040.

Entry information

Entry name

DAD1_YEAST

Accession

Primary (citable) accession number: Q12248
Secondary accession number(s): D6VS02

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	November 1, 1996
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program