Q12242 (RUP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UBA domain-containing protein RUP1

Gene names

Name:	RUP1
Ordered Locus Names:	YOR138C
ORF Names:	YOR3332c

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	671 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Modulates the activity of the RSP5 HECT ubiquitin-protein ligase through its mediation of the interaction between RSP5 and the deubiquitinase UBP2. Involved in regulation of cell wall homeostasis. Ref.7 Ref.8
Subunit structure	Forms a ternary complex with RSP5 and UBP2.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 5930 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 UBA domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from mutant phenotype Ref.7. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
UBP2	Q01476	3	EBI-38794,EBI-19826

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 671	671	UBA domain-containing protein RUP1		PRO_0000269644
Regions
	Domain	1 – 41	41	UBA
	Coiled coil	432 – 501	70	Potential
	Compositional bias	354 – 399	46	Glu-rich
Amino acid modifications
	Modified residue	56	1	Phosphoserine Ref.9
	Modified residue	126	1	Phosphoserine Ref.9
	Modified residue	129	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	Q12242 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 69E950C4537597FB	FASTA	671	75,361
	10 20 30 40 50 60 MMDNQAVKSL LEMGIPHEVA VDALQRTGGN LEAAVNFIFS NELPEQAEMG EENDGSQPRI 70 80 90 100 110 120 SENKIVAGTK PCDVPNNGDQ DIDMPDVSGV DVDYDDDEDI TDERSGSNST SGCRVTAQNY 130 140 150 160 170 180 DRYSISETSI PPPSYSIVQH NEFKSNVGDP TVVLPLPLNS LIESYFGLFA LLTAVYFPHV 190 200 210 220 230 240 FLKPDFKDLN YRADWFKGSS FTEPKYRLAY CEAEDGSTTS EIVLASGPNE GLQPHLLWQL 250 260 270 280 290 300 QKLISVVNTR KCERAFVSAK VFTSSLEPQL RSKLADSEHL YEVLPAFIKS LAVDLEMCPG 310 320 330 340 350 360 IRDRETRSLF ISSALHTPNK NEPPMETFLS LFHFLPEEYD SNLYKMFNVL LYPEEEEEEE 370 380 390 400 410 420 DVIRGGEQEE ARYVEPENTL KEVAPVLTIL FNELETNTES VSLPNGVDIP LEFYPQLYTK 430 440 450 460 470 480 QCKDQLIRHI ISKRKQARTR SRCLLQEINE LKSYQGKNIS TILESTLAYL QTIPDDANNE 490 500 510 520 530 540 AAKQIASLKD TLNSARAAKM EEYKDLASKL HGEWNLSHPE THIINTAKQL GLIENPYILT 550 560 570 580 590 600 MAALSPYSYF IRSRNGAWSW IQSNTLGTEF KVKKCSSPSV VQEAIKHGTK YASETPLMFI 610 620 630 640 650 660 YCEEGKIPTE EVVAEALKSN SGCLKFAEDD QNSLKTLRSQ FFDGMGDPEQ ATNNINNGND 670 NDNDDDIDSD N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing and analysis of 130 kb from yeast chromosome XV." Voss H., Benes V., Andrade M.A., Valencia A., Rechmann S., Teodoru C., Schwager C., Paces V., Sander C., Ansorge W. Yeast 13:655-672(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme." Kee Y., Lyon N., Huibregtse J.M. EMBO J. 24:2414-2424(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH RSP5 AND UBP2.
[8]	"The Ubp2 deubiquitinating enzyme modulates Rsp5-dependent K63-linked polyubiquitin conjugates in Saccharomyces cerevisiae." Kee Y., Munoz W., Lyon N., Huibregtse J.M. J. Biol. Chem. 281:36724-36731(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-126 AND SER-129, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94335 Genomic DNA. Translation: CAA64056.1. Z75046 Genomic DNA. Translation: CAA99337.1. AY692617 Genomic DNA. Translation: AAT92636.1. BK006948 Genomic DNA. Translation: DAA10911.1.
PIR	S61693.
RefSeq	NP_014781.1. NM_001183557.1.
3D structure databases
ProteinModelPortal	Q12242.
SMR	Q12242. Positions 3-41.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1986N.
IntAct	Q12242. 6 interactions.
MINT	MINT-410462.
STRING	4932.YOR138C.
Proteomic databases
PaxDb	Q12242.
PeptideAtlas	Q12242.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR138C; YOR138C; YOR138C.
GeneID	854306.
KEGG	sce:YOR138C.
Organism-specific databases
CYGD	YOR138c.
SGD	S000005664. RUP1.
Phylogenomic databases
eggNOG	NOG85652.
HOGENOM	HOG000154228.
OMA	HFLPEEY.
OrthoDB	EOG4WWVTB.
Gene expression databases
Genevestigator	Q12242.
Family and domain databases
InterPro	IPR024871. Rup1. IPR009060. UBA-like. IPR000449. UBA/transl_elong_EF1B_N. IPR015940. UBA/transl_elong_EF1B_N_euk. [Graphical view]
PANTHER	PTHR10175. PTHR10175. 1 hit.
Pfam	PF00627. UBA. 1 hit. [Graphical view]
SMART	SM00165. UBA. 1 hit. [Graphical view]
SUPFAM	SSF46934. UBA_like. 1 hit.
PROSITE	PS50030. UBA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976319.

Entry information

Entry name

RUP1_YEAST

Accession

Primary (citable) accession number: Q12242
Secondary accession number(s): D6W2J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program